Gene/Protein Characteristic Table for KIAA0132
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Product ID ORK00420
Accession No D50922
Description kelch-like ECH-associated protein 1, transcript variant 2
Clone name ha01449s1
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (2513 bp)
Predicted protein sequence (637 aa)
Flexi ORF Clone FXC00420
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0132 by Kazusa Mouse cDNA Project
Note We replaced ha01449, former representative clones for KIAA0132 with ha01449s1. (2008/12/19)
Features of the cloned cDNA sequence
Description

Length: 2513 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 526 bp
Genome contig ID gi42406306r_10357802
PolyA signal sequence
(AATAAA,-29)
+----*----+----*----+----*----+----
AAGGAAAATAAAGAACAGACTAACTAGTGTCTTTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACCCTGGCTCTGGGCTGGAGGCCTGAAACCGGGGGCCAGAAAGGGCTCTT
Features of the protein sequence
Description

Length: 637 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_512371 0 100.0 kelch-like ECH-...
Pan troglodytes
Q14145 0 100.0 Kelch-like ECH-...
Homo sapiens
BAG51647 0 99.8 unnamed protein...
Homo sapiens
BAG09615 0 99.8 kelch-like ECH-...
synthetic construct
Q5R774 0 99.5 Kelch-like ECH-...
Pongo abelii
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB040923 1.9e-72 34.6 KIAA1490
AB032955 6.4e-71 37.4 KIAA1129
AB051474 7.3e-70 34.0 KIAA1687
AB037799 5.4e-61 31.8 KIAA1378
AB018338 1.1e-57 35.7 KIAA0795
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR006651 576 589 PR00501 Kelch motif
IPR006651 593 607 PR00501 Kelch motif
HMMPfam IPR013069 80 192 PF00651 BTB/POZ
IPR011705 197 299 PF07707 BTB/Kelch-associated
IPR006652 328 372 PF01344 Kelch repeat
IPR011498 374 423 PF07646 Kelch
IPR006652 374 423 PF01344 Kelch repeat
IPR011498 425 470 PF07646 Kelch
IPR006652 425 470 PF01344 Kelch repeat
IPR011498 472 517 PF07646 Kelch
IPR006652 472 517 PF01344 Kelch repeat
IPR011498 519 564 PF07646 Kelch
IPR006652 519 564 PF01344 Kelch repeat
IPR011498 566 611 PF07646 Kelch
IPR006652 566 611 PF01344 Kelch repeat
HMMSmart IPR000210 90 192 SM00225 BTB
ProfileScan IPR000210 90 162 PS50097 BTB
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 19
Experimental conditions
Panel name Genebridge 4
Primer_f TTACGACCCAGATACAGACAC
Primer_r TTCTGCTGGTCAATCTGCTTC
PCR product length 114 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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