KIAA0115

Product ID	ORK00413
Accession No	D29643
Description	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Clone name	ha00643
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (1668 bp) Predicted protein sequence (456 aa)
HaloTag ORF Clone	FHC00413
Flexi ORF Clone	FXC00413
Source	Myeloblast cell line (KG-1)

Length: 1668 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	191 bp
Genome contig ID	gi89161185r_20751268
PolyA signal sequence (TATAAA,-24)	+--------+--------+----*----+---- GATAATTTTTATATAAAAGAAGTTTTTCCACTTTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATTGCTAAAAGTGGCATTTTTCCTATGTGCAGTCACTCCTCTCATTTCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	20851268	20860587	11	99.6	Perfect prediction

Length: 456 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAA23670	5.8e-197	99.8	oligosaccharylt...	Homo sapiens
AAH02594	9.2e-197	99.8	Dolichyl-diphos...	Homo sapiens
AAP36629	9.2e-197	99.8	dolichyl-diphos...	synthetic construct
XP_001161661	3.8e-196	99.6	dolichyl-diphos...	Pan troglodytes
CAH73476	5.2e-196	99.6	dolichyl-diphos...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005013	26	455	PF03345	Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	TAARAWALFWLLLPLLGAVCASG	44	PRIMARY	23
SOSUI2	2	431	SAFPMMLGLFIFSIVFLHM	449	PRIMARY	19

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	GGGGGTTATTAGGATTGGTGG
Primer_r	ACCCCTTGGCTCTCAGTGTTG
PCR product length	103 bp
PCR conditions	95 °C15 sec66 °C120 sec30 cycles