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Gene/Protein Characteristic Table for FLJ00036

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Product ID	ORK04009
Accession No	AK024446
Clone name	as00036
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4538 bp) Predicted protein sequence (706 aa)
Source	Human spleen

Length: 4538 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 706 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	612	638	PD000006	ABC transporter
HMMPfam	IPR001140	99	412	PF00664	ABC transporter
	IPR003439	487	669	PF00005	ABC transporter
HMMSmart	IPR003593	486	670	SM00382	AAA ATPase
ProfileScan	IPR001687	489	514	PS50101	ATP/GTP-binding site motif A (P-loop)
	IPR003439	596	666	PS50100	ABC transporter
ScanRegExp	IPR003439	596	610	PS00211	ABC transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	93	AVGQGLALAILFSLLLMQATRNA	115	PRIMARY	23
	2	142	PASMGLFSPQLLLFSPGNLYIPV	164	SECONDARY	23
	3	180	FYLTVYATIAGVNSLCTLLRAVL	202	PRIMARY	23
	4	263	NAAGLLGLLAVLGSGLPWLLLLL	285	PRIMARY	23
	5	368	RLQLMGAAVVSAIAGIALVQHQ	389	SECONDARY	22

RT-PCR-ELISA

Experimental conditions

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