Gene/Protein Characteristic Table for KIAA1674
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Product ID ORK00885
Accession No AB051461
Description leucine rich repeat containing 27, transcript variant 1
Clone name fg03838
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6382 bp)
Predicted protein sequence (538 aa)
Flexi ORF Clone FXC00885
Source Human fetal brain
Rouge ID mKIAA1674 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6382 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 4684 bp
Genome contig ID gi89161187f_133895694
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CTTTGCAGATGAGCCACATAGCCTAGAAATATCTG
Flanking genome sequence
(147720 - 147769)
----+----*----+----*----+----*----+----*----+----*
AAAAAAGAAAAAGTTATGTCATGAATGCATAAAATATACAGACCATACAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 f 133995694 134043412 11 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 538 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9C0I9 5.3e-180 100.0 Leucine-rich re...
Homo sapiens
BAG53467 1.1e-179 99.8 unnamed protein...
Homo sapiens
XP_001144456 1.2e-170 95.8 leucine rich re...
Pan troglodytes
XP_001092022 1.3e-160 90.6 similar to leuc...
Macaca mulatta
CAI13019 1.1e-155 100.0 novel protein [...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001611 76 98 PF00560 Leucine-rich repeat
IPR001611 100 121 PF00560 Leucine-rich repeat
IPR001611 123 144 PF00560 Leucine-rich repeat
HMMSmart IPR003591 74 97 SM00369 Leucine-rich repeat
IPR003591 98 121 SM00369 Leucine-rich repeat
IPR003591 122 144 SM00369 Leucine-rich repeat
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CAAGGAGGATGAGCTGCTGTC
Primer_r AAGATGATGCCTCCAACACCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 10
Experimental conditions
Panel name GeneBridge 4
Primer_f CCACAATGCCACCTGACTGAG
Primer_r GTGGCCTTTGGTGTAACTCTC
PCR product length 216 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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