Gene/Protein Characteristic Table for KIAA0740
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Product ID ORK00122
Accession No AB018283
Description Rho-related BTB domain containing 1, transcript variant 1
Clone name hk03989s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4388 bp)
Predicted protein sequence (696 aa)
Flexi ORF Clone FXC00122
Source Human adult brain
Rouge ID mKIAA0740 by Kazusa Mouse cDNA Project
Note We replaced hk03989, former representative clones for KIAA0740 with hk03989s1. (2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 4388 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2040 bp
Genome contig ID gi89161187r_62199206
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
TATTTCACAAAAAGCCAATAAAATTGTTTAGCTAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAATGCCTCCAGTTTTGTTTTAAGGACAGACATCATATTGTGTGTGTGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 r 62299206 62373930 11 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 696 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_507810 0 99.4 Rho-related BTB...
Pan troglodytes
CAH91062 0 98.9 hypothetical pr...
Pongo abelii
XP_001094923 0 97.8 Rho-related BTB...
Macaca mulatta
XP_001503521 0 94.4 similar to Rho-...
Equus caballus
XP_546116 0 93.1 similar to Rho-...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB018260 2.1e-87 68.0 KIAA0717
AB020685 5.7e-14 24.1 KIAA0878
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001806 15 36 PR00449 Ras GTPase
IPR001806 71 93 PR00449 Ras GTPase
IPR001806 131 144 PR00449 Ras GTPase
IPR001806 185 207 PR00449 Ras GTPase
HMMPfam IPR013753 100 209 PF00071 Ras
IPR013069 260 457 PF00651 BTB/POZ
IPR013069 475 583 PF00651 BTB/POZ
HMMSmart IPR003577 12 210 SM00173 Ras small GTPase
IPR003579 15 210 SM00175 Ras small GTPase
IPR003578 17 210 SM00174 Ras small GTPase
IPR000210 266 457 SM00225 BTB/POZ-like
IPR000210 485 583 SM00225 BTB/POZ-like
HMMTigr IPR005225 12 188 TIGR00231 Small GTP-binding protein domain
ProfileScan IPR000210 266 427 PS50097 BTB/POZ-like
IPR000210 485 552 PS50097 BTB/POZ-like
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AGAAGTAGGCTAAGGCAGACC
Primer_r TCAGAACGAACTCACAATAGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 10
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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