RHOBTB1 ORF clone FHC00122

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00122

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Clone info.

Species

human

Clone Name

pFN21AA0740 Sequence (with vector) Restriction Map

Product ID

FHC00122

Flexi Clone PID

FXC00122

ORF Sequence

DNA sequence (2088 bp)
Protein sequence (696 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384519]

Gene

KIAA0740

Symbol

RHOBTB1

Description

Rho-related BTB domain containing 1, transcript variant 1

Original Clone ID

hk03989s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2088 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 696 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7261.1 0 100.0 RHOBTB1 CCDS6034.1 4.7e-94 68.0 RHOBTB2 CCDS55211.1 4.7e-94 68.0 RHOBTB2 CCDS55210.1 4.8e-94 68.0 RHOBTB2 CCDS2795.1 3.5e-15 39.0 RHOA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 15 36 PR00449 Ras GTPase IPR001806 71 93 PR00449 Ras GTPase IPR001806 131 144 PR00449 Ras GTPase IPR001806 185 207 PR00449 Ras GTPase HMMPfam IPR013753 16 207 PF00071 Ras IPR013069 404 455 PF00651 BTB/POZ IPR013069 477 581 PF00651 BTB/POZ HMMSmart IPR003577 12 210 SM00173 Ras small GTPase IPR003579 15 210 SM00175 Ras small GTPase IPR003578 17 210 SM00174 Small GTPase IPR000210 266 457 SM00225 BTB/POZ-like IPR000210 485 583 SM00225 BTB/POZ-like ProfileScan IPR003578 8 207 PS51420 Small GTPase IPR000210 266 427 PS50097 BTB/POZ-like IPR000210 485 552 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055651 696 607351 100.0 100.0 XP_016872490 696 607351 100.0 100.0 NP_001229288 696 607351 100.0 100.0 XP_016872489 696 607351 100.0 100.0 XP_011538726 696 607351 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0740 Download

>KIAA0740  2088 bp
ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTG
GGTGACAATGCCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTC
ACGCAGTATCAGCTGCTGGCCACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGC
GTGTGCCAGGAGGTCTTGGAGCGTTCTCGGGATGTTGTTGATGAAGTGAGTGTTTCTCTC
AGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGACGCTTTGCATATGGCAGGTCT
GATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCATGTGAAAAGC
ATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC
CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCA
AGGCCCATAAAGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAA
CTTGGCTTACCATACTATGAAACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTT
GACAATGCAATCCGAGCAGCGCTGATTTCCCGCAGGCACCTGCAATTCTGGAAATCCCAC
CTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCCTACCTCCAAAAGCCCCTCCA
CCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGCCTGTTTACTG
GACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA
CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGT
GAAGAATCCCCAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTC
CAGGGGCGGATATTGAGTGTCGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATT
CCTCAGGCCGACCAGTGGAAGTCTTCAAACAAGAGCCTGGTGGAGGCTCTGGGGCTGGAA
GCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGATGGAGTAAGGGGTTCATTGGC
ATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCATGACTGTGGTC
AGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG
GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTC
GAGATGTTCGATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAAC
CAGGAGATTACGAAAGCCTTTCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGC
AAGGGAACGTTCTCGGACGTGACATTTAAATTGGACGATGGAGCCATCAGTGCCCACAAG
CCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGTTCGGGGGGTCATTTGTGGAA
AGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCAAGCAGTATTG
GATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT
GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTT
CAGGAGTTGACCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTAC
TTGGAATTGGCTCAGTTTCACAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATC
TGCACCAACTACAACAGTGTATGCTCCAAGTTCCGTAAGGAAATCAAATCAAAATCTGCA
GACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTGTGTGGTACCTGAAGGAAGAA
GATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACTAAATAAGCAT
CGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AA0740 Download

>KIAA0740  696 aa
MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYR
VCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKS
MWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKE
LGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPP
PVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMEC
EESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSNKSLVEALGLE
AEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYT
GQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLS
KGTFSDVTFKLDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVL
DYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSY
LELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEE
DHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0740 Download

>pFN21AA0740  6793 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGCTGACATGGACTACGAAAGACCCAACGT
TGAAACTATCAAATGTGTGGTCGTGGGTGACAATGCCGTGGGGAAGACGCGCTTGATCTG
TGCCAGGGCGTGCAACACCACACTCACGCAGTATCAGCTGCTGGCCACCCACGTGCCAAC
AGTGTGGGCGATTGACCAGTACCGCGTGTGCCAGGAGGTCTTGGAGCGTTCTCGGGATGT
TGTTGATGAAGTGAGTGTTTCTCTCAGGCTTTGGGATACTTTTGGTGATCATCACAAAGA
CAGACGCTTTGCATATGGCAGGTCTGATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCC
CAATTCCCTAAATCATGTGAAAAGCATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCG
AACACCCGTTATCCTTGTTGGGTGCCAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGT
TAATCGAGCCAGGCGCCCGTTAGCAAGGCCCATAAAGAGAGGGGATATTTTGCCCCCAGA
AAAAGGCCGAGAGGTAGCAAAGGAACTTGGCTTACCATACTATGAAACAAGCGTGTTTGA
CCAGTTTGGTATCAAGGATGTGTTTGACAATGCAATCCGAGCAGCGCTGATTTCCCGCAG
GCACCTGCAATTCTGGAAATCCCACCTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACC
CTTCCTACCTCCAAAAGCCCCTCCACCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGG
GACAAATGAAGCTGCCTGTTTACTGGACAATCCTCTATGTGCCGATGTTCTGTTCATCCT
TCAGGACCAGGAACACATCTTTGCACATCGAATTTACCTCGCTACCTCTTCTTCCAAATT
TTATGATCTGTTTTTAATGGAATGTGAAGAATCCCCAAATGGGAGTGAAGGAGCCTGTGA
GAAAGAGAAGCAGAGCAGAGATTTCCAGGGGCGGATATTGAGTGTCGACCCAGAGGAAGA
AAGGGAGGAGGGCCCGCCTAGGATTCCTCAGGCCGACCAGTGGAAGTCTTCAAACAAGAG
CCTGGTGGAGGCTCTGGGGCTGGAAGCCGAGGGTGCAGTTCCTGAGACACAGACTTTGAC
CGGATGGAGTAAGGGGTTCATTGGCATGCACAGGGAAATGCAAGTCAACCCCATTTCAAA
GCGGATGGGGCCCATGACTGTGGTCAGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCG
GACCCTGCTCCAGTTTCTTTATACGGGACAACTGGATGAAAAGGAAAAGGATTTGGTGGG
CCTGGCTCAGATCGCAGAGGTCCTCGAGATGTTCGATTTGAGGATGATGGTGGAAAACAT
CATGAACAAGGAAGCCTTCATGAACCAGGAGATTACGAAAGCCTTTCACGTAAGGAAAGC
CAATCGGATAAAAGAGTGTCTCAGCAAGGGAACGTTCTCGGACGTGACATTTAAATTGGA
CGATGGAGCCATCAGTGCCCACAAGCCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGC
CATGTTCGGGGGGTCATTTGTGGAAAGTGCCAACAGTGAGGTGTATCTCCCGAACATAAA
CAAGATATCAATGCAAGCAGTATTGGATTATCTCTATACCAAGCAGTTGTCTCCTAACTT
GGATCTGGACCCGCTGGAATTAATTGCCTTGGCAAACAGATTTTGCCTGCCACACTTGGT
TGCACTTGCAGAACAGCATGCCGTTCAGGAGTTGACCAAAGCCGCCACGAGTGGCGTGGG
CATTGACGGAGAAGTGCTCTCTTACTTGGAATTGGCTCAGTTTCACAATGCCCACCAGTT
GGCCGCCTGGTGTTTGCACCACATCTGCACCAACTACAACAGTGTATGCTCCAAGTTCCG
TAAGGAAATCAAATCAAAATCTGCAGACAACCAGGAATACTTCGAGCGGCACCGCTGGCC
CCCTGTGTGGTACCTGAAGGAAGAAGATCACTACCAGCGTGTGAAAAGGGAACGAGAGAA
GGAAGATATTGCACTAAATAAGCATCGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATC
TCCAGCAGTGGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT