|
Order Kazusa clone(s) from : |
| Product ID | ORK00104 |
|---|---|
| Accession No | AB011162 |
| Description | intraflagellar transport 140 |
| Clone name | hj02755 |
| Vector information | |
| cDNA sequence | DNA sequence (4962 bp) Predicted protein sequence (1468 aa) |
|
Flexi ORF Clone |
FXC00104
|
| Source | Human adult brain |
| Rouge ID |
mKIAA0590
by Kazusa Mouse cDNA Project
|
Length: 4962 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1468 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001680 | 99 | 137 | PF00400 | WD40 repeat |
| HMMSmart | IPR001680 | 61 | 95 | SM00320 | WD40 repeat |
| IPR001680 | 97 | 137 | SM00320 | WD40 repeat | |
| IPR001680 | 320 | 358 | SM00320 | WD40 repeat | |
| ProfileScan | IPR001680 | 63 | 146 | PS50294 | WD40 repeat |
| IPR001680 | 105 | 146 | PS50082 | WD40 repeat |
RT-PCR
|
|---|
Experimental conditions| Primer_f | CATGTCTGTGTTGGAATACGC |
|---|---|
| Primer_r | GAATACGATGGAAGGGTGAAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 16
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CATGTCTGTGTTGGAATACGC |
| Primer_r | GAATACGATGGAAGGGTGAAC |
| PCR product length | 178 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |