Gene/Protein Characteristic Table for KIAA0118
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Product ID ORK06560
Accession No D42087
Description RAB21, member RAS oncogene family
Clone name ha00793
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (1413 bp)
Predicted protein sequence (161 aa)
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0118 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 1413 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 927 bp
Genome contig ID gi89161190f_70350638
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TTATCTGCTTTTACAAGCGCAAGGTGCAAAAATAT
Flanking genome sequence
(116011 - 116060)
----+----*----+----*----+----*----+----*----+----*
ATACAATAGTCTCATTGATGACTGTAAAGTGAATTAACATTTGGTGATTA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 12 f 70449880 70466647 6 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 161 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001117520 2e-66 100.0 similar to Ras-...
Macaca mulatta
XP_522469 2e-66 100.0 similar to GTP-...
Pan troglodytes
Q9UL25 2e-66 100.0 Ras-related pro...
Homo sapiens
P55745 7.2e-66 98.8 Ras-related pro...
Canis lupus fam...
XP_001490458 1.4e-65 98.1 similar to RAB2...
Equus caballus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001806 59 72 PR00449 Ras GTPase
IPR001806 94 116 PR00449 Ras GTPase
HMMPfam IPR013753 1 118 PF00071 Ras
HMMSmart IPR003578 1 119 SM00174 Ras small GTPase
IPR003577 1 119 SM00173 Ras small GTPase
IPR003579 1 119 SM00175 Ras small GTPase
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 12
Experimental conditions
Panel name Genebridge 4
Primer_f TGATAGAAACAGCACAAGTGG
Primer_r TCCTGGCTAACCTGGTGAAAC
PCR product length 582 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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