KIAA0091

Product ID	ORK00405
Accession No	D42053
Description	membrane-bound transcription factor peptidase, site 1
Clone name	ha01032
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4338 bp) Predicted protein sequence (1058 aa)
HaloTag ORF Clone	FHC00405
Flexi ORF Clone	FXC00405
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0091 by Kazusa Mouse cDNA Project

Length: 4338 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	683 bp
Genome contig ID	gi51511732r_82544872
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- ATTTTCAATAAATATGTATTACCCGTCTGAAGCTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAGATACTGTGTAACGGACTCTTTCCACTTGTCCCATTTCTCTTTGATG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	r	82644872	82708012	23	99.9	Perfect prediction

Length: 1058 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q14703	100.0	Membrane-bound ...	Homo sapiens
BAF84462	99.9	unnamed protein...	Homo sapiens
XP_001112150	99.7	membrane-bound ...	Macaca mulatta
CAH93329	99.3	hypothetical pr...	Pongo abelii
XP_850300	97.1	similar to Memb...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000209	215	234	PR00723	Peptidase S8 and S53
	IPR000209	251	264	PR00723	Peptidase S8 and S53
	IPR000209	417	433	PR00723	Peptidase S8 and S53
HMMPfam	IPR000209	198	470	PF00082	Peptidase S8 and S53
ScanRegExp	IPR000209	255	265	PS00137	Peptidase S8 and S53
ScanRegExp	IPR000209	418	428	PS00138	Peptidase S8 and S53

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	YSLVTMKLVNIWLLLLVVLLCG	23	PRIMARY	22
	2	52	EFSSTVVEYEYIVAFNGYFTAK	73	SECONDARY	22
	3	1006	QTIPVFAFLGAMVVLAFFVVQIN	1028	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	ACCACAACCTCCGCTATCCAC
Primer_r	GAAGATGACGAGCGAGAGGCC
PCR product length	287 (2.0k) bp
PCR conditions	95 °C15 sec66 °C120 sec30 cycles