KIAA0018

Product ID	ORK00004
Accession No	D13643
Description	24-dehydrocholesterol reductase
Clone name	hk07284
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4268 bp) Predicted protein sequence (553 aa)
HaloTag ORF Clone	FHC00004
Flexi ORF Clone	FXC00004
Source	Human adult brain
Rouge ID	mKIAA0018 by Kazusa Mouse cDNA Project
Note	We replaced ha00517, former representative clones for KIAA0018 with hk07284. (2001/10/06)

Length: 4268 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	2605 bp
Genome contig ID	gi89161185r_54987889
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TGCTTCTGTTTAAATAAAAGTGGCCTGGAAGCTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* CGTTTGTCGTTTCTTGCTTGGGGGACCAAAATGTGGAGTCAGATCTGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	55087889	55125493	9	99.2	Perfect prediction

Length: 553 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001108186	98.2	similar to 24-d...	Macaca mulatta
Q15392	100.0	24-dehydrochole...	Homo sapiens
AAP36155	100.0	24-dehydrochole...	synthetic construct
XP_001153873	99.8	24-dehydrochole...	Pan troglodytes
Q60HC5	99.4	24-dehydrochole...	Macaca fascicularis

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006094	151	240	PF01565	FAD linked oxidase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	40	PAVSLAVCALLFLLWVRLKGLEF	62	PRIMARY	23
SOSUI2	2	74	LFLLPLSLIFDIYYYVRAWVVFK	96	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TTGGCCAGAAGGATGAATAC
Primer_r	AGTGCCTGTGGCTGTAAGAG
PCR product length	156 bp
PCR conditions	95 °C15 sec58 °C60 sec30 cycles