FLJ00138

Product ID	ORK06825
Accession No	AK074067
Clone name	sh03216
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5595 bp) Predicted protein sequence (309 aa)
Source	Human spleen
Rouge ID	mFLJ00138 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 309 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84893	7.9e-120	100.0	FLJ00138 protei...	Homo sapiens
Q5VZ18	1.1e-119	100.0	SH2 domain-cont...	Homo sapiens
AAI43766	2.3e-119	99.7	Src homology 2 ...	Homo sapiens
XP_524890	4.7e-119	99.4	hypothetical pr...	Pan troglodytes
XP_001929477	1.9e-109	91.1	similar to Src ...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000980	209	291	PD000093	SH2 motif
FPrintScan	IPR000980	209	223	PR00401	SH2 motif
	IPR000980	228	238	PR00401	SH2 motif
	IPR000980	274	288	PR00401	SH2 motif
HMMPfam	IPR000980	209	285	PF00017	SH2 motif
HMMSmart	IPR000980	207	291	SM00252	SH2 motif
ProfileScan	IPR000980	209	304	PS50001	SH2 motif

Primer_f
Primer_r
PCR conditions	test