##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AscI Asp718I AviII AvrII BamHI
BbeI BciVI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BssHII ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
FseI FspI HgaI Hin1I KasI
KpnI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SbfI SfiI SfoI SgrAI
SnaBI SphI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 3415 AACGTT AA^CGTT
AflIII 3961 ACATGT A^C[GA][CT]GT
AhdI 79 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 815 AGCGCT AGC^GCT
ApaI 341 GGGCCC GGGCC^C
AspEI 79 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4782 GCAGTGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4143 TGATCA T^GATCA
BlpI 482 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 482 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 3730 TGTACA T^GTACA
BspLU11I 3961 ACATGT A^CATGT
BsrBI 1688 GAGCGG CCGCTC(-3/-3)
BsrGI 3730 TGTACA T^GTACA
BssSI 2952 CACGAG CACGAG(-5/-1)
BstAPI 445 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 699 TTCGAA TT^CGAA
BstEII 689 GGTTACC G^GT[ACGT]ACC
BstPI 689 GGTTACC G^GT[ACGT]ACC
CelII 482 GCTGAGC GC^T[ACGT]AGC
Eam1105I 79 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 815 AGCGCT AGC^GCT
EcoO65I 689 GGTTACC G^GT[ACGT]ACC
EcoRI 1956 GAATTC G^AATTC
EcoRV 5393 GATATC GAT^ATC
EcoT22I 4640 ATGCAT ATGCA^T
FbaI 4143 TGATCA T^GATCA
HaeII 815 AGCGCT [GA]GCGC^[CT]
HpaI 2532 GTTAAC GTT^AAC
KspI 405 CCGCGG CCGC^GG
NaeI 378 GCCGGC GCC^GGC
NgoMIV 378 GCCGGC G^CCGGC
NsiI 4640 ATGCAT ATGCA^T
NspI 3961 ACATGT [GA]CATG^[CT]
NspV 699 TTCGAA TT^CGAA
PacI 5300 TTAATTAA TTAAT^TAA
Psp1406I 3415 AACGTT AA^CGTT
SacI 3972 GAGCTC GAGCT^C
SacII 405 CCGCGG CCGC^GG
SalI 663 GTCGAC G^TCGAC
SapI 532 GAAGAGC GCTCTTC(1/4)
ScaI 2413 AGTACT AGT^ACT
SfuI 699 TTCGAA TT^CGAA
SmaI 598 CCCGGG CCC^GGG
SspBI 3730 TGTACA T^GTACA
StuI 4209 AGGCCT AGG^CCT
SwaI 5477 ATTTAAAT ATTT^AAAT
XmaCI 598 CCCGGG C^CCGGG
XmaI 598 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 363 CGCG CG^CG
406 CGCG
AflII 2312 CTTAAG C^TTAAG
4421 CTTAAG
Alw44I 911 GTGCAC G^TGCAC
3504 GTGCAC
AocI 1511 CCTCAGG CC^T[ACGT]AGG
1791 CCTAAGG
ApaLI 911 GTGCAC G^TGCAC
3504 GTGCAC
AspI 1085 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3201 GACCCTGTC
BbrPI 1615 CACGTG CAC^GTG
2826 CACGTG
BfrI 2312 CTTAAG C^TTAAG
4421 CTTAAG
BglII 2186 AGATCT A^GATCT
2669 AGATCT
BseRI 503 GAGGAG GAGGAG(10/8)
3688 GAGGAG
BsmBI 29 GAGACG CGTCTC(1/5)
1083 GAGACG
BsmI 3979 GAATGC GAATGC(1/-1)
4673 GCATTC
BspHI 1454 TCATGA T^CATGA
2347 TCATGA
Bst1107I 2592 GTATAC GTA^TAC
4342 GTATAC
BstUI 363 CGCG CG^CG
406 CGCG
BstZ17I 2592 GTATAC GTA^TAC
4342 GTATAC
Bsu36I 1511 CCTCAGG CC^T[ACGT]AGG
1791 CCTAAGG
Eco81I 1511 CCTCAGG CC^T[ACGT]AGG
1791 CCTAAGG
HincII 663 GTCGAC GT[CT]^[GA]AC
2532 GTTAAC
HindII 663 GTCGAC GT[CT]^[GA]AC
2532 GTTAAC
HindIII 920 AAGCTT A^AGCTT
3482 AAGCTT
MvnI 363 CGCG CG^CG
406 CGCG
PflFI 1085 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3201 GACCCTGTC
PmaCI 1615 CACGTG CAC^GTG
2826 CACGTG
PmlI 1615 CACGTG CAC^GTG
2826 CACGTG
RcaI 1454 TCATGA T^CATGA
2347 TCATGA
Tth111I 1085 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3201 GACCCTGTC
XbaI 1553 TCTAGA T^CTAGA
3404 TCTAGA
XcmI 180 CCAAAGATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
279 CCAAGGACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SpeI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI