LGI2 ORF clone pFN21ASDA1916

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Halotag ORF Clone pFN21ASDA1916

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Clone info.

Species

human

Clone Name

pFN21ASDA1916 Sequence (with vector) Restriction Map

Product ID

FHC00949

Flexi Clone PID

FXC00949

ORF Sequence

DNA sequence (1635 bp)
Protein sequence (545 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384324]

Gene

KIAA1916

Symbol

LGI2

Alias : LGIL2

Description

leucine-rich repeat LGI family, member 2

Original Clone ID

hg01117

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1635 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 545 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3431.1 2.1e-200 99.8 LGI2 CCDS7431.1 5.6e-115 56.1 LGI1 CCDS6025.1 5.3e-108 51.7 LGI3 CCDS81490.1 1.6e-92 50.2 LGI1 CCDS12444.1 6e-79 41.2 LGI4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001611 110 132 PF00560 Leucine-rich repeat IPR001611 135 156 PF00560 Leucine-rich repeat IPR005492 218 261 PF03736 Leucine-rich glioma-inactivated IPR005492 264 306 PF03736 Leucine-rich glioma-inactivated IPR005492 310 358 PF03736 Leucine-rich glioma-inactivated IPR005492 360 402 PF03736 Leucine-rich glioma-inactivated IPR005492 406 450 PF03736 Leucine-rich glioma-inactivated IPR005492 451 493 PF03736 Leucine-rich glioma-inactivated IPR005492 497 536 PF03736 Leucine-rich glioma-inactivated HMMSmart IPR003591 84 107 SM00369 Leucine-rich repeat IPR003591 108 131 SM00369 Leucine-rich repeat IPR003591 132 155 SM00369 Leucine-rich repeat IPR000483 167 216 SM00082 Cysteine-rich flanking region ProfileScan NULL 1 22 PS51257 NULL IPR009039 218 261 PS50912 EAR IPR009039 264 307 PS50912 EAR IPR009039 310 358 PS50912 EAR IPR009039 359 403 PS50912 EAR IPR009039 406 450 PS50912 EAR IPR009039 451 494 PS50912 EAR IPR009039 497 540 PS50912 EAR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 GCGALGLLLLLLGAACLIPRSAQ 29 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060646 545 608301 99.8 100.0 NP_005088 557 600512,604619 56.1 98.0 NP_644807 548 608302 51.7 99.1 NP_001295205 509 600512,604619 50.2 98.0 NP_644813 537 608303 41.2 98.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1916 Download

>KIAA1916  1635 bp
ATGGCGCTGCGGAGAGGCGGCTGCGGAGCGCTCGGGCTGCTGCTGCTGCTGCTGGGCGCC
GCGTGCCTGATACCGCGGAGCGCGCAGGTGAGGCGGCTGGCGCGCTGCCCCGCCACTTGC
AGCTGTACCAAGGAGTCTATCATCTGCGTGGGCTCTTCCTGGGTGCCCAGGATCGTGCCG
GGCGACATCAGCTCCCTGAGCCTGGTAAATGGGACGTTTTCAGAAATCAAGGACCGAATG
TTTTCCCATCTGCCTTCTCTGCAGCTGCTATTGCTGAATTCTAACTCATTCACGATCATC
CGGGATGATGCTTTTGCTGGACTTTTTCATCTTGAATACCTGTTCATTGAAGGGAACAAA
ATAGAAACCATTTCAAGAAATGCCTTTCGTGGCCTCCGTGACCTGACTCACCTTTCTTTG
GCCAATAACCACATAAAAGCACTACCAAGGGATGTCTTCAGTGATTTAGACTCTCTGATT
GAACTAGATTTGAGGGGTAATAAATTTGAATGTGACTGCAAAGCCAAGTGGCTATACCTG
TGGTTGAAGATGACAAATTCCACCGTTTCTGATGTGCTGTGTATTGGTCCACCAGAGTAT
CAGGAAAAGAAGCTAAATGACGTGACCAGCTTTGACTATGAATGCACAACTACAGATTTT
GTTGTTCATCAGACTTTACCCTACCAGTCGGTTTCAGTGGATACGTTCAACTCCAAGAAC
GATGTGTACGTGGCCATCGCGCAGCCCAGCATGGAGAACTGCATGGTGCTGGAGTGGGAC
CACATTGAAATGAATTTCCGGAGCTATGACAACATTACAGGTCAGTCCATCGTGGGCTGT
AAGGCCATTCTCATCGATGATCAGGTCTTTGTGGTGGTAGCCCAGCTCTTCGGTGGCTCT
CACATTTACAAATACGACGAGAGTTGGACCAAATTTGTCAAATTCCAAGACATAGAGGTC
TCTCGCATTTCCAAGCCCAATGACATCGAGCTGTTTCAGATCGACGACGAGACGTTCTTT
GTCATCGCAGACAGCTCAAAGGCTGGTCTGTCCACAGTTTATAAATGGAACAGCAAAGGA
TTCTATTCTTACCAGTCACTGCACGAGTGGTTCAGGGACACGGATGCGGAGTTTGTTGAT
ATCGATGGAAAATCGCATCTCATCCTGTCCAGCCGCTCCCAGGTCCCCATCATCCTCCAG
TGGAATAAAAGCTCTAAGAAGTTTGTCCCCCATGGTGACATCCCCAACATGGAGGACGTA
CTGGCTGTGAAGAGCTTCCGAATGCAAAATACCCTCTACCTTTCCCTTACCCGCTTCATC
GGGGACTCCCGGGTCATGAGGTGGAACAGTAAGCAGTTTGTGGAGATCCAAGCTCTTCCA
TCCCGGGGGGCCATGACCCTGCAGCCCTTTTCTTTTAAAGATAATCACTACCTGGCCCTG
GGGAGTGACTATACATTCTCTCAGATATACCAGTGGGATAAAGAGAAGCAGCTATTCAAA
AAGTTTAAGGAGATTTATGTGCAGGCGCCTCGTTCATTCACAGCTGTCTCCACCGACAGG
AGAGATTTCCTTTTTGCATCCAGTTTCAAAGGGAAAACAAAGATTTTTGAACATATAATT
GTTGACTTAAGTTTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1916 Download

>KIAA1916  545 aa
MALRRGGCGALGLLLLLLGAACLIPRSAQVRRLARCPATCSCTKESIICVGSSWVPRIVP
GDISSLSLVNGTFSEIKDRMFSHLPSLQLLLLNSNSFTIIRDDAFAGLFHLEYLFIEGNK
IETISRNAFRGLRDLTHLSLANNHIKALPRDVFSDLDSLIELDLRGNKFECDCKAKWLYL
WLKMTNSTVSDVLCIGPPEYQEKKLNDVTSFDYECTTTDFVVHQTLPYQSVSVDTFNSKN
DVYVAIAQPSMENCMVLEWDHIEMNFRSYDNITGQSIVGCKAILIDDQVFVVVAQLFGGS
HIYKYDESWTKFVKFQDIEVSRISKPNDIELFQIDDETFFVIADSSKAGLSTVYKWNSKG
FYSYQSLHEWFRDTDAEFVDIDGKSHLILSSRSQVPIILQWNKSSKKFVPHGDIPNMEDV
LAVKSFRMQNTLYLSLTRFIGDSRVMRWNSKQFVEIQALPSRGAMTLQPFSFKDNHYLAL
GSDYTFSQIYQWDKEKQLFKKFKEIYVQAPRSFTAVSTDRRDFLFASSFKGKTKIFEHII
VDLSL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1916 Download

>pFN21ASDA1916  6364 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGCTGCGGAGAGG
CGGCTGCGGAGCGCTCGGGCTGCTGCTGCTGCTGCTGGGCGCCGCGTGCCTGATACCGCG
GAGCGCGCAGGTGAGGCGGCTGGCGCGCTGCCCCGCCACTTGCAGCTGTACCAAGGAGTC
TATCATCTGCGTGGGCTCTTCCTGGGTGCCCAGGATCGTGCCGGGCGACATCAGCTCCCT
GAGCCTGGTAAATGGGACGTTTTCAGAAATCAAGGACCGAATGTTTTCCCATCTGCCTTC
TCTGCAGCTGCTATTGCTGAATTCTAACTCATTCACGATCATCCGGGATGATGCTTTTGC
TGGACTTTTTCATCTTGAATACCTGTTCATTGAAGGGAACAAAATAGAAACCATTTCAAG
AAATGCCTTTCGTGGCCTCCGTGACCTGACTCACCTTTCTTTGGCCAATAACCACATAAA
AGCACTACCAAGGGATGTCTTCAGTGATTTAGACTCTCTGATTGAACTAGATTTGAGGGG
TAATAAATTTGAATGTGACTGCAAAGCCAAGTGGCTATACCTGTGGTTGAAGATGACAAA
TTCCACCGTTTCTGATGTGCTGTGTATTGGTCCACCAGAGTATCAGGAAAAGAAGCTAAA
TGACGTGACCAGCTTTGACTATGAATGCACAACTACAGATTTTGTTGTTCATCAGACTTT
ACCCTACCAGTCGGTTTCAGTGGATACGTTCAACTCCAAGAACGATGTGTACGTGGCCAT
CGCGCAGCCCAGCATGGAGAACTGCATGGTGCTGGAGTGGGACCACATTGAAATGAATTT
CCGGAGCTATGACAACATTACAGGTCAGTCCATCGTGGGCTGTAAGGCCATTCTCATCGA
TGATCAGGTCTTTGTGGTGGTAGCCCAGCTCTTCGGTGGCTCTCACATTTACAAATACGA
CGAGAGTTGGACCAAATTTGTCAAATTCCAAGACATAGAGGTCTCTCGCATTTCCAAGCC
CAATGACATCGAGCTGTTTCAGATCGACGACGAGACGTTCTTTGTCATCGCAGACAGCTC
AAAGGCTGGTCTGTCCACAGTTTATAAATGGAACAGCAAAGGATTCTATTCTTACCAGTC
ACTGCACGAGTGGTTCAGGGACACGGATGCGGAGTTTGTTGATATCGATGGAAAATCGCA
TCTCATCCTGTCCAGCCGCTCCCAGGTCCCCATCATCCTCCAGTGGAATAAAAGCTCTAA
GAAGTTTGTCCCCCATGGTGACATCCCCAACATGGAGGACGTACTGGCTGTGAAGAGCTT
CCGAATGCAAAATACCCTCTACCTTTCCCTTACCCGCTTCATCGGGGACTCCCGGGTCAT
GAGGTGGAACAGTAAGCAGTTTGTGGAGATCCAAGCTCTTCCATCCCGGGGGGCCATGAC
CCTGCAGCCCTTTTCTTTTAAAGATAATCACTACCTGGCCCTGGGGAGTGACTATACATT
CTCTCAGATATACCAGTGGGATAAAGAGAAGCAGCTATTCAAAAAGTTTAAGGAGATTTA
TGTGCAGGCGCCTCGTTCATTCACAGCTGTCTCCACCGACAGGAGAGATTTCCTTTTTGC
ATCCAGTTTCAAAGGGAAAACAAAGATTTTTGAACATATAATTGTTGACTTAAGTTTGTA
CGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT