HELZ2 ORF clone pFN21ASDA1769

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA1769

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Clone info.

Species

human

Clone Name

pFN21ASDA1769 Sequence (with vector) Restriction Map

Product ID

FHC01636

Flexi Clone PID

FXC01636

ORF Sequence

DNA sequence (6240 bp)
Protein sequence (2080 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384289]

Gene

KIAA1769

Symbol

HELZ2

Alias : PDIP-1, PRIC285

Description

helicase with zinc finger 2, transcriptional coactivator, transcript variant 2

Original Clone ID

af00171

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 6240 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 2080 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13527.1 0 99.8 HELZ2 CCDS33508.1 0 99.7 HELZ2 CCDS42374.1 1.8e-38 41.0 HELZ CCDS82192.1 1.8e-38 41.0 HELZ CCDS10214.1 8.2e-19 24.6 DIS3L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001900 765 1122 PF00773 Ribonuclease II/R HMMSmart IPR001900 765 1124 SM00955 Ribonuclease II/R
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_208384 2080 611265 99.8 100.0 NP_001032412 2649 611265 99.7 99.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1769 Download

>KIAA1769  6240 bp
ATGAGCTCCTCCCCCTCCCGCAGTGCCGCCGACATCTACATCCGGGAGTATTTCCACAGC
CACGTCAGCGGCGGCCACCCCGAGGCCACTCCTCTCCGTGTGATGTACACGGACCGGCCG
CTGAGCCAGACGGACCCAGTCACGCTGCAGTACTGTTGCCTGACCGACGACCGCCAGGCT
TTCCGCCCGCCCACACGGGCAGAGCTGGCGCGGCACCGCGTGGTGGTCACCACCACCTCC
CAGGCCCGTGAGCTCAGGGTGCCGGTCGGCTTCTTCTCCCACATTCTCATCGATGAGGCG
GCCCAGATGCTGGAGTGCGAGGCCCTCACCCCGCTGGCCTACGCCTCGCACGGCACCCGC
CTCGTGCTGGCGGGCGACCACATGCAGGTCACACCCCGGCTGTTCAGTGTGGCCAGGGCC
CGGGCGGCCGAGCACACGCTGCTGCACCGCCTCTTCCTGTGCTACCAGCAGGAGACTCAC
GAGGTGGCGCGGCAGAGCCGCCTGGTCTTCCACGAGAACTACCGCTGCACGGACGCCATT
GTCAGCTTCATCTCGCGGCACTTCTACGTGGCCAAGGGCAACCCCATCCACGCCAGGGGC
AAGGTTCCGCCCCACCCCCGGCACTACCCGCTCATGTTCTGCCACGTGGCGGGCAACCCA
GACCGGGACATGTCCATGGCGTCCTGGCTGAATCTGGCTGAGATTGCGCAGGTCGTCGAG
AAGGTGCAGGAGGCCTACAACACCTGGCCCAGCTGCTGGGGCGGCCGTGAGCAGAGGTGC
ATCTGTGTCGTTTCCCACGGTGCCCAGGTCAGTGCACTGAGGCAGGAGCTGAGGAGGCGG
GACCTAGGCCAGGTGTCTGTCGGCAGTTTTGAGATCCTGCCAGGGCGGCAGTTCCGGGTC
GTGGTGCTCAGCACTGTGCACACCTGCCAGAGCCTGCTCAGCCCTGGGGCACTGGCCCCT
GAGTTCTTCACCGACGCCCGCGTGCTCAACACCGTCCTGACCCGCGCCCAGTCCCAGCTG
GTGGTAGTGGGGGACGCCGTGGCCCTCTGCTCCTTCGGGGCCTGCGGCAAGCTCTGGGAG
AGCTTCATCCGTGAGTGCGTGGAGCGGCACAGTGTCTGCCCCGAGGGCCTGTCCATGGAG
CAGGTCGAGCAGGGTGTGGCGCAGAGACGGCGCTGGCCTCCCCGAGGCACACAGGCTGGG
GCAGCGGGGAACTGGGAGGCTGCCCCAGAGCCAGTAGGGGACCTGGCCGAGGAGCAGGCG
GCTGTGGTGACGGCCATGGTGAAGGCAGAGCCGGGAGATGAGGCTCTGAGCCCAGCATCC
CGTGACATCACGGCAACCACAGCGCAGACGGAGGCTGCGGCAGCACCAGCAGGAGACGCA
GTGAAGGAAGACGTGGTGCCCGGGGCCTGTGCGGCAGGAGCGGCTGCTGCAGCGGGCGTG
GAGTCCACGGAGGCTGAGGATGCAGAGGCTGACTTCTGGCCGTGGGACGGGGAGCTCAAC
GCTGACGACGCCATCCTACGGGAGCTTCTGGACGAGAGCCAGAAGGTGATGGTGACCGTC
GGGGAGGACGGGCTGCTGGACACCGTCGCCAGGCCCGAGTCCCTGCAGCAGGCCCGGCTG
TACGAGAACCTGCCCCCGGCTGCGCTACGGAAGCTGCTGCGCGCGGAGCCCGAGCGGTAC
CGCCACTGCTCTTTCGTGCCAGAGACCTTCGAGCGGGCGTCAGCCATCCCGCTGGACGAT
GCCTCCTCGGGCCCCATCCAGGTCAGGGGCCGCCTGGACTGTGGGATGGCCTTCGCCGGG
GATGAGGTGCTGGTGCAGCTCCTTTCGGGAGACAAGGCGCCCGAGGGGCGGCTTCGGGGC
CGCGTGCTGGGCGTGCTGAAGAGGAAGAGGCACGAGCTGGCGTTTGTGTGCCGCATGGAC
ACGTGGGACCCGCGCATCATGGTCCCCATCAATGGCTCCGTGACCAAGATCTTCGTGGCC
GAGCTGAAGGACCCATCGCAGGTCCCCATCTACAGCCTCCGGAAGGGCCGGCTGCAGCGT
GTGGGGCTTGAGAGGCTCACCGCCGAGGCCCGGCACAGCCGGCTCTTCTGGGTCCAAATT
GTCCTGTGGCGGCAAGGCTTCTACTACCCGCTGGGCATCGTCCGGGAGGTGCTGCCTGAG
GCCAGCACCTGGGAGCAGGGCCTCCGCATCCTCGGCCTGGAGTACAGCTTGAGGGTGCCC
CCGTCGGACCAGGCCACCATCACCAAGGTGCTGCAGAAATACCACACGGAGCTTGGCCGG
GTTGCCGGCCGCCGAGAGGACTGCCGCGCCTTCTTGACCTTCACTGTGGACCCCCAGGGC
GCCTGCAACCTCGATGATGCCCTCAGTGTCCGAGACCTGGGTCCCAGGTGCGAGGTGGCT
GTGCACATCACTGATGTGGCCAGCTTCGTGCCCAGGGACGGGGTGCTGGACGTGGAGGCG
CGAAGGCAGGGCGCTGCGTTCTATGCCCCCGGCAGGGAGCCAGTGCCCATGCTGCCGGCC
AGCCTCTGCCAGGACGTCCTCAGCCTCCTGCCTGGCCGGGACCGCCTGGCCATCTCCCTG
TTCCTCACCATGGAGAAGGCCAGTGGCCAGCTGAAGAGCCTGCGCTTTGCACCCTCCGTG
GTCCAGTCTGACCGCCAGCTGTCCTACGAGGAGGCGGAGGAGGTGATCAGGCAGCACCCG
GGTGCCGGCCGTGAGCTGCCGGCCCGCCTGGACTCCGTGGACGCCTGCGTCGTGGCCGCG
TGCTACTTCTCTCGGCTGCTGCGCCGGCACCGCCTGCGGTCCGACTGCTTCTATGAGCAG
CCGGACGAGGACGGCACCCTGGGCTTCCGCGCGGCCCACATCATGGTGAAGGAGTACATG
ATTCAGTTTAATAGGCTCGTGGCTGAGTTCCTGGTGGGCAGCGAGTGCACGCGGACGGTC
ACGCCTCTGCGGTGGCAGCCAGCACCCCGCAGCCAGCAGCTCAAGGCCCTGTGTGAGAAG
CATGGGGACCGGGTGCCCCTGTCACTGCACCTCGGCCACCACCTGCACGGCGGCGGGGGC
AGTCCCCCCGACACGCGGCTGCACCTCCTGGCCTCCCTCTGGAAGCAGGTCCAGTTTGCT
GCCCGCACCCAGGACTACGAACAGATGGTGGACTTGGTCACCACGGACGACATGCACCCA
TTCCTGGCTCCTGCAGGCCGCGACCTCCGCAAGGCCCTGGAGCGCTCGGCGTTCGGCCGC
TGCGCCCGGGGCCACCAGCAGCAGGGCGGCCACTACTCGCTGCAGGTGGACTGGTACACG
TGGGCCACCTCGCCCATCCGCAGGTACCTGGACGTGGTGTTGCAGCGGCAGATCCTGCTG
GCGCTGGGCCATGGGGGCTCTGCCTACTCTGCCAGGGACATCGATGGGCTCTGCCAGGCC
TTCAGCCTCCAGCACGCACTTGCCCAGAGCTATCAGCGGCGGGCGCGCAGCCTGCACCTG
GCCGTGCAGCTCAAGGCCCAGCCTCTGGACAAGCTGGGCTTCGTGGTGGACGTGGAGGCG
GGCTCCCGCTGCTTCCGGCTGCTCTTCCCCAGCAACCGGGAGACGCTGCCTGACCCCTGC
CCCGTCCCCTACGGCTCCCTGCAGCTGGCCGAGCACCCCCACGCCCTGGCAGGCCGGCCG
GGCCTGCGGCTCCTGTGGCGGCGCCGTGTCTACTCAGCGCAGGGATCCAGCCCGCCCCTG
CCACTGCCTGGCACTGTGCCGGACCCACACACCCTGGCCGTGGAGACGGCCCTGTGGAAG
CAGCTGCTGGAGCTGGTGGAGCTGCAGCGCTGGCCGGAGGCGGCTGCTCTCATCCAGGAG
AAGGGCGAGGCGTCCCAGCGGCGGGAGCTGGTGCAGGTGCAGCGGAGCCACTGTGGCCAT
TTCCTGGAGGTGGCCCGGGAGCTGGGCAGTGGGGACACCCTGCAGGTGCAGCTCGGCACC
AGCCTGCAGCACGGCTTCCTGGTACCGAGCCCTCAGCTCTGGACGGTGGCACCGGGCTTC
AGCCTCTGCCTGGAGCACGTGGAGCGGCCCGGAGACTGCTTCTCAGGCCGTGTGTACCGG
GCCCCGAGGGACCGGTACCGCGACGTGGATGAGTACGCCTGCGTGTGGGAACCATTCTGC
GCCCTGGAGTCGGCCACCGGCGCGGTTGCCGAGAATGACTCCGTCACACTTCAGCACCTG
AGTGTCTCCTGGGAGGCGTCACGGACGCCGCAGGGGCAGCTGCAGGGCGCCTTCCGCCTG
GAGGCCGCCTTCCTCGAGGAGAACTGTGCCGACATCAACTTCAGCTGCTGCTACCTCTGC
ATCCGGCTCGAGGGGCTGCTGGCTCCCACGGCCAGCCCACGCCCCGGGCCCAGCAGCCTC
GGCCCTGGCCTGAATGTTGACCCCGGCACGTATACCTGGGTGGCCCACGGGCAGACGGAG
GACTGGGACCAGGAGCGCCGGGCAGACCGGCAGGAGGCTCCCAGACGGGTGCACCTCTTC
GTCCACCACATGGGCATGGAGAAGGTTCCGGAAGAGGTGCTGAGGCCGGGCACCCTGTTC
ACCGTTGAGCTGCTGCCCAAGCAGCTTCCTGATCTCCGCAAGGAGGAAGCCGTGCGTGGA
CTAGAGGAGGCGTCCCCGCTGGTCACCAGCATCGCACTGGGCCGGCCTGTCCCGCAGCCC
CTCTGCAGAGTGATCCCCAGCAGGTTCCTGGAGCGGCAGACCTACAACATCCCCGGAGGC
CGCCACAAGCTGAACCCCAGCCAGAACGTGGCGGTCAGGGAGGCTCTGGAGAAGCCTTTC
ACGGTCATTCAGGGCCCACCAGGTACAGGGAAGACGATCGTGGGCCTCCACATCGTATTC
TGGTTTCATAAATCAAACCAGGAGCAGGTGCAGCCCGGAGGCCCCCCCCGTGGGGAGAAG
CGGCTGGGGGGTCCCTGCATCTTGTACTGCGGCCCCTCCAACAAGTCGGTGGATGTCCTG
GCAGGACTGCTCCTGAGAAGGATGGAGCTGAAGCCCCTCCGTGTGTACAGTGAGCAGGCT
GAGGCCAGCGAGTTCCCAGTGCCGCGTGTGGGCAGCAGGAAGCTGCTCAGGAAGAGCCCC
CGGGAGGGGAGGCCGAACCAGAGCCTCAGGAGCATCACCCTGCACCACCGGATCCGGCAG
GCCCCCAACCCTTACTCGTCGGAAATCAAGGCCTTTGACACCCGGCTGCAGAGAGGGGAG
CTCTTCTCCAGGGAGGACCTGGTCTGGTACAAGAAGGTCTTGTGGGAGGCTCGGAAGTTC
GAGCTGGACCGGCATGAGGTCATCCTCTGCACCTGCTCCTGTGCAGCCTCTGCCAGCCTC
AAAATCCTGGACGTGAGGCAGATCCTTGTTGACGAGGCAGGCATGGCCACGGAACCTGAA
ACCCTCATCCCCCTGGTGCAGTTCCCACAGGCCGAGAAGGTGGTTCTTCTCGGAGACCAC
AAGCAGCTGCGGCCTGTGGTCAAGAATGAGCGGCTGCAAAACCTGGGTCTGGACCGGTCT
CTGTTCGAGCGGTACCACGAGGACGCACATATGCTGGACACTCAGTACCGCATGCATGAG
GGCATCTGTGCCTTCCCCTCTGTGGCGTTCTACAAGAGCAAGCTGAAGACGTGGCAGGGC
CTGAGGAGGCCGCCCAGTGTCCTGGGCCACGCTGGCAAGGAGAGCTGTCCTGTCATCTTT
GGCCACGTGCAGGGCCACGAGCGGAGCCTGCTGGTGTCCACGGACGAAGGGAATGAGAAC
TCCAAGGCCAACCTGGAGGAGGTGGCTGAGGTGGTCCGTATCACCAAGCAGCTGACCCTG
GGGAGGACCGTAGAGCCCCAGGACATCGCCGTCCTCACGCCCTACAACGCGCAGGCCTCT
GAGATCAGCAAGGCCCTTCGGCGAGAGGGCATCGCCGGGGTGGCCGTGTCCTCCATCACC
AAGAGCCAGGGGAGCGAGTGGCGCTATGTGCTGGTGAGCACCGTCCGCACCTGTGCCAAG
AGCGACCTGGACCAGCGGCCCACCAAGAGCTGGCTCAAGAAGTTTCTGGGCTTCGTTGTG
GACCCCAACCAAGTGAACGTGGCTGTCACGCGGGCCCAGGAGGGGCTCTGCCTGATCGGA
GACCACCTCCTTCTGCGCTGCTGCCCCCTCTGGCGTAGCCTCCTGGACTTCTGCGAGGCT
CAGCAGACCCTCGTGCCTGCCGGCCAGGTGCGCGTCTGCAGGAGGCCAACTATGCCTTCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1769 Download

>KIAA1769  2080 aa
MSSSPSRSAADIYIREYFHSHVSGGHPEATPLRVMYTDRPLSQTDPVTLQYCCLTDDRQA
FRPPTRAELARHRVVVTTTSQARELRVPVGFFSHILIDEAAQMLECEALTPLAYASHGTR
LVLAGDHMQVTPRLFSVARARAAEHTLLHRLFLCYQQETHEVARQSRLVFHENYRCTDAI
VSFISRHFYVAKGNPIHARGKVPPHPRHYPLMFCHVAGNPDRDMSMASWLNLAEIAQVVE
KVQEAYNTWPSCWGGREQRCICVVSHGAQVSALRQELRRRDLGQVSVGSFEILPGRQFRV
VVLSTVHTCQSLLSPGALAPEFFTDARVLNTVLTRAQSQLVVVGDAVALCSFGACGKLWE
SFIRECVERHSVCPEGLSMEQVEQGVAQRRRWPPRGTQAGAAGNWEAAPEPVGDLAEEQA
AVVTAMVKAEPGDEALSPASRDITATTAQTEAAAAPAGDAVKEDVVPGACAAGAAAAAGV
ESTEAEDAEADFWPWDGELNADDAILRELLDESQKVMVTVGEDGLLDTVARPESLQQARL
YENLPPAALRKLLRAEPERYRHCSFVPETFERASAIPLDDASSGPIQVRGRLDCGMAFAG
DEVLVQLLSGDKAPEGRLRGRVLGVLKRKRHELAFVCRMDTWDPRIMVPINGSVTKIFVA
ELKDPSQVPIYSLRKGRLQRVGLERLTAEARHSRLFWVQIVLWRQGFYYPLGIVREVLPE
ASTWEQGLRILGLEYSLRVPPSDQATITKVLQKYHTELGRVAGRREDCRAFLTFTVDPQG
ACNLDDALSVRDLGPRCEVAVHITDVASFVPRDGVLDVEARRQGAAFYAPGREPVPMLPA
SLCQDVLSLLPGRDRLAISLFLTMEKASGQLKSLRFAPSVVQSDRQLSYEEAEEVIRQHP
GAGRELPARLDSVDACVVAACYFSRLLRRHRLRSDCFYEQPDEDGTLGFRAAHIMVKEYM
IQFNRLVAEFLVGSECTRTVTPLRWQPAPRSQQLKALCEKHGDRVPLSLHLGHHLHGGGG
SPPDTRLHLLASLWKQVQFAARTQDYEQMVDLVTTDDMHPFLAPAGRDLRKALERSAFGR
CARGHQQQGGHYSLQVDWYTWATSPIRRYLDVVLQRQILLALGHGGSAYSARDIDGLCQA
FSLQHALAQSYQRRARSLHLAVQLKAQPLDKLGFVVDVEAGSRCFRLLFPSNRETLPDPC
PVPYGSLQLAEHPHALAGRPGLRLLWRRRVYSAQGSSPPLPLPGTVPDPHTLAVETALWK
QLLELVELQRWPEAAALIQEKGEASQRRELVQVQRSHCGHFLEVARELGSGDTLQVQLGT
SLQHGFLVPSPQLWTVAPGFSLCLEHVERPGDCFSGRVYRAPRDRYRDVDEYACVWEPFC
ALESATGAVAENDSVTLQHLSVSWEASRTPQGQLQGAFRLEAAFLEENCADINFSCCYLC
IRLEGLLAPTASPRPGPSSLGPGLNVDPGTYTWVAHGQTEDWDQERRADRQEAPRRVHLF
VHHMGMEKVPEEVLRPGTLFTVELLPKQLPDLRKEEAVRGLEEASPLVTSIALGRPVPQP
LCRVIPSRFLERQTYNIPGGRHKLNPSQNVAVREALEKPFTVIQGPPGTGKTIVGLHIVF
WFHKSNQEQVQPGGPPRGEKRLGGPCILYCGPSNKSVDVLAGLLLRRMELKPLRVYSEQA
EASEFPVPRVGSRKLLRKSPREGRPNQSLRSITLHHRIRQAPNPYSSEIKAFDTRLQRGE
LFSREDLVWYKKVLWEARKFELDRHEVILCTCSCAASASLKILDVRQILVDEAGMATEPE
TLIPLVQFPQAEKVVLLGDHKQLRPVVKNERLQNLGLDRSLFERYHEDAHMLDTQYRMHE
GICAFPSVAFYKSKLKTWQGLRRPPSVLGHAGKESCPVIFGHVQGHERSLLVSTDEGNEN
SKANLEEVAEVVRITKQLTLGRTVEPQDIAVLTPYNAQASEISKALRREGIAGVAVSSIT
KSQGSEWRYVLVSTVRTCAKSDLDQRPTKSWLKKFLGFVVDPNQVNVAVTRAQEGLCLIG
DHLLLRCCPLWRSLLDFCEAQQTLVPAGQVRVCRRPTMPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1769 Download

>pFN21ASDA1769  10969 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGAGCTCCTCCCCCTC
CCGCAGTGCCGCCGACATCTACATCCGGGAGTATTTCCACAGCCACGTCAGCGGCGGCCA
CCCCGAGGCCACTCCTCTCCGTGTGATGTACACGGACCGGCCGCTGAGCCAGACGGACCC
AGTCACGCTGCAGTACTGTTGCCTGACCGACGACCGCCAGGCTTTCCGCCCGCCCACACG
GGCAGAGCTGGCGCGGCACCGCGTGGTGGTCACCACCACCTCCCAGGCCCGTGAGCTCAG
GGTGCCGGTCGGCTTCTTCTCCCACATTCTCATCGATGAGGCGGCCCAGATGCTGGAGTG
CGAGGCCCTCACCCCGCTGGCCTACGCCTCGCACGGCACCCGCCTCGTGCTGGCGGGCGA
CCACATGCAGGTCACACCCCGGCTGTTCAGTGTGGCCAGGGCCCGGGCGGCCGAGCACAC
GCTGCTGCACCGCCTCTTCCTGTGCTACCAGCAGGAGACTCACGAGGTGGCGCGGCAGAG
CCGCCTGGTCTTCCACGAGAACTACCGCTGCACGGACGCCATTGTCAGCTTCATCTCGCG
GCACTTCTACGTGGCCAAGGGCAACCCCATCCACGCCAGGGGCAAGGTTCCGCCCCACCC
CCGGCACTACCCGCTCATGTTCTGCCACGTGGCGGGCAACCCAGACCGGGACATGTCCAT
GGCGTCCTGGCTGAATCTGGCTGAGATTGCGCAGGTCGTCGAGAAGGTGCAGGAGGCCTA
CAACACCTGGCCCAGCTGCTGGGGCGGCCGTGAGCAGAGGTGCATCTGTGTCGTTTCCCA
CGGTGCCCAGGTCAGTGCACTGAGGCAGGAGCTGAGGAGGCGGGACCTAGGCCAGGTGTC
TGTCGGCAGTTTTGAGATCCTGCCAGGGCGGCAGTTCCGGGTCGTGGTGCTCAGCACTGT
GCACACCTGCCAGAGCCTGCTCAGCCCTGGGGCACTGGCCCCTGAGTTCTTCACCGACGC
CCGCGTGCTCAACACCGTCCTGACCCGCGCCCAGTCCCAGCTGGTGGTAGTGGGGGACGC
CGTGGCCCTCTGCTCCTTCGGGGCCTGCGGCAAGCTCTGGGAGAGCTTCATCCGTGAGTG
CGTGGAGCGGCACAGTGTCTGCCCCGAGGGCCTGTCCATGGAGCAGGTCGAGCAGGGTGT
GGCGCAGAGACGGCGCTGGCCTCCCCGAGGCACACAGGCTGGGGCAGCGGGGAACTGGGA
GGCTGCCCCAGAGCCAGTAGGGGACCTGGCCGAGGAGCAGGCGGCTGTGGTGACGGCCAT
GGTGAAGGCAGAGCCGGGAGATGAGGCTCTGAGCCCAGCATCCCGTGACATCACGGCAAC
CACAGCGCAGACGGAGGCTGCGGCAGCACCAGCAGGAGACGCAGTGAAGGAAGACGTGGT
GCCCGGGGCCTGTGCGGCAGGAGCGGCTGCTGCAGCGGGCGTGGAGTCCACGGAGGCTGA
GGATGCAGAGGCTGACTTCTGGCCGTGGGACGGGGAGCTCAACGCTGACGACGCCATCCT
ACGGGAGCTTCTGGACGAGAGCCAGAAGGTGATGGTGACCGTCGGGGAGGACGGGCTGCT
GGACACCGTCGCCAGGCCCGAGTCCCTGCAGCAGGCCCGGCTGTACGAGAACCTGCCCCC
GGCTGCGCTACGGAAGCTGCTGCGCGCGGAGCCCGAGCGGTACCGCCACTGCTCTTTCGT
GCCAGAGACCTTCGAGCGGGCGTCAGCCATCCCGCTGGACGATGCCTCCTCGGGCCCCAT
CCAGGTCAGGGGCCGCCTGGACTGTGGGATGGCCTTCGCCGGGGATGAGGTGCTGGTGCA
GCTCCTTTCGGGAGACAAGGCGCCCGAGGGGCGGCTTCGGGGCCGCGTGCTGGGCGTGCT
GAAGAGGAAGAGGCACGAGCTGGCGTTTGTGTGCCGCATGGACACGTGGGACCCGCGCAT
CATGGTCCCCATCAATGGCTCCGTGACCAAGATCTTCGTGGCCGAGCTGAAGGACCCATC
GCAGGTCCCCATCTACAGCCTCCGGAAGGGCCGGCTGCAGCGTGTGGGGCTTGAGAGGCT
CACCGCCGAGGCCCGGCACAGCCGGCTCTTCTGGGTCCAAATTGTCCTGTGGCGGCAAGG
CTTCTACTACCCGCTGGGCATCGTCCGGGAGGTGCTGCCTGAGGCCAGCACCTGGGAGCA
GGGCCTCCGCATCCTCGGCCTGGAGTACAGCTTGAGGGTGCCCCCGTCGGACCAGGCCAC
CATCACCAAGGTGCTGCAGAAATACCACACGGAGCTTGGCCGGGTTGCCGGCCGCCGAGA
GGACTGCCGCGCCTTCTTGACCTTCACTGTGGACCCCCAGGGCGCCTGCAACCTCGATGA
TGCCCTCAGTGTCCGAGACCTGGGTCCCAGGTGCGAGGTGGCTGTGCACATCACTGATGT
GGCCAGCTTCGTGCCCAGGGACGGGGTGCTGGACGTGGAGGCGCGAAGGCAGGGCGCTGC
GTTCTATGCCCCCGGCAGGGAGCCAGTGCCCATGCTGCCGGCCAGCCTCTGCCAGGACGT
CCTCAGCCTCCTGCCTGGCCGGGACCGCCTGGCCATCTCCCTGTTCCTCACCATGGAGAA
GGCCAGTGGCCAGCTGAAGAGCCTGCGCTTTGCACCCTCCGTGGTCCAGTCTGACCGCCA
GCTGTCCTACGAGGAGGCGGAGGAGGTGATCAGGCAGCACCCGGGTGCCGGCCGTGAGCT
GCCGGCCCGCCTGGACTCCGTGGACGCCTGCGTCGTGGCCGCGTGCTACTTCTCTCGGCT
GCTGCGCCGGCACCGCCTGCGGTCCGACTGCTTCTATGAGCAGCCGGACGAGGACGGCAC
CCTGGGCTTCCGCGCGGCCCACATCATGGTGAAGGAGTACATGATTCAGTTTAATAGGCT
CGTGGCTGAGTTCCTGGTGGGCAGCGAGTGCACGCGGACGGTCACGCCTCTGCGGTGGCA
GCCAGCACCCCGCAGCCAGCAGCTCAAGGCCCTGTGTGAGAAGCATGGGGACCGGGTGCC
CCTGTCACTGCACCTCGGCCACCACCTGCACGGCGGCGGGGGCAGTCCCCCCGACACGCG
GCTGCACCTCCTGGCCTCCCTCTGGAAGCAGGTCCAGTTTGCTGCCCGCACCCAGGACTA
CGAACAGATGGTGGACTTGGTCACCACGGACGACATGCACCCATTCCTGGCTCCTGCAGG
CCGCGACCTCCGCAAGGCCCTGGAGCGCTCGGCGTTCGGCCGCTGCGCCCGGGGCCACCA
GCAGCAGGGCGGCCACTACTCGCTGCAGGTGGACTGGTACACGTGGGCCACCTCGCCCAT
CCGCAGGTACCTGGACGTGGTGTTGCAGCGGCAGATCCTGCTGGCGCTGGGCCATGGGGG
CTCTGCCTACTCTGCCAGGGACATCGATGGGCTCTGCCAGGCCTTCAGCCTCCAGCACGC
ACTTGCCCAGAGCTATCAGCGGCGGGCGCGCAGCCTGCACCTGGCCGTGCAGCTCAAGGC
CCAGCCTCTGGACAAGCTGGGCTTCGTGGTGGACGTGGAGGCGGGCTCCCGCTGCTTCCG
GCTGCTCTTCCCCAGCAACCGGGAGACGCTGCCTGACCCCTGCCCCGTCCCCTACGGCTC
CCTGCAGCTGGCCGAGCACCCCCACGCCCTGGCAGGCCGGCCGGGCCTGCGGCTCCTGTG
GCGGCGCCGTGTCTACTCAGCGCAGGGATCCAGCCCGCCCCTGCCACTGCCTGGCACTGT
GCCGGACCCACACACCCTGGCCGTGGAGACGGCCCTGTGGAAGCAGCTGCTGGAGCTGGT
GGAGCTGCAGCGCTGGCCGGAGGCGGCTGCTCTCATCCAGGAGAAGGGCGAGGCGTCCCA
GCGGCGGGAGCTGGTGCAGGTGCAGCGGAGCCACTGTGGCCATTTCCTGGAGGTGGCCCG
GGAGCTGGGCAGTGGGGACACCCTGCAGGTGCAGCTCGGCACCAGCCTGCAGCACGGCTT
CCTGGTACCGAGCCCTCAGCTCTGGACGGTGGCACCGGGCTTCAGCCTCTGCCTGGAGCA
CGTGGAGCGGCCCGGAGACTGCTTCTCAGGCCGTGTGTACCGGGCCCCGAGGGACCGGTA
CCGCGACGTGGATGAGTACGCCTGCGTGTGGGAACCATTCTGCGCCCTGGAGTCGGCCAC
CGGCGCGGTTGCCGAGAATGACTCCGTCACACTTCAGCACCTGAGTGTCTCCTGGGAGGC
GTCACGGACGCCGCAGGGGCAGCTGCAGGGCGCCTTCCGCCTGGAGGCCGCCTTCCTCGA
GGAGAACTGTGCCGACATCAACTTCAGCTGCTGCTACCTCTGCATCCGGCTCGAGGGGCT
GCTGGCTCCCACGGCCAGCCCACGCCCCGGGCCCAGCAGCCTCGGCCCTGGCCTGAATGT
TGACCCCGGCACGTATACCTGGGTGGCCCACGGGCAGACGGAGGACTGGGACCAGGAGCG
CCGGGCAGACCGGCAGGAGGCTCCCAGACGGGTGCACCTCTTCGTCCACCACATGGGCAT
GGAGAAGGTTCCGGAAGAGGTGCTGAGGCCGGGCACCCTGTTCACCGTTGAGCTGCTGCC
CAAGCAGCTTCCTGATCTCCGCAAGGAGGAAGCCGTGCGTGGACTAGAGGAGGCGTCCCC
GCTGGTCACCAGCATCGCACTGGGCCGGCCTGTCCCGCAGCCCCTCTGCAGAGTGATCCC
CAGCAGGTTCCTGGAGCGGCAGACCTACAACATCCCCGGAGGCCGCCACAAGCTGAACCC
CAGCCAGAACGTGGCGGTCAGGGAGGCTCTGGAGAAGCCTTTCACGGTCATTCAGGGCCC
ACCAGGTACAGGGAAGACGATCGTGGGCCTCCACATCGTATTCTGGTTTCATAAATCAAA
CCAGGAGCAGGTGCAGCCCGGAGGCCCCCCCCGTGGGGAGAAGCGGCTGGGGGGTCCCTG
CATCTTGTACTGCGGCCCCTCCAACAAGTCGGTGGATGTCCTGGCAGGACTGCTCCTGAG
AAGGATGGAGCTGAAGCCCCTCCGTGTGTACAGTGAGCAGGCTGAGGCCAGCGAGTTCCC
AGTGCCGCGTGTGGGCAGCAGGAAGCTGCTCAGGAAGAGCCCCCGGGAGGGGAGGCCGAA
CCAGAGCCTCAGGAGCATCACCCTGCACCACCGGATCCGGCAGGCCCCCAACCCTTACTC
GTCGGAAATCAAGGCCTTTGACACCCGGCTGCAGAGAGGGGAGCTCTTCTCCAGGGAGGA
CCTGGTCTGGTACAAGAAGGTCTTGTGGGAGGCTCGGAAGTTCGAGCTGGACCGGCATGA
GGTCATCCTCTGCACCTGCTCCTGTGCAGCCTCTGCCAGCCTCAAAATCCTGGACGTGAG
GCAGATCCTTGTTGACGAGGCAGGCATGGCCACGGAACCTGAAACCCTCATCCCCCTGGT
GCAGTTCCCACAGGCCGAGAAGGTGGTTCTTCTCGGAGACCACAAGCAGCTGCGGCCTGT
GGTCAAGAATGAGCGGCTGCAAAACCTGGGTCTGGACCGGTCTCTGTTCGAGCGGTACCA
CGAGGACGCACATATGCTGGACACTCAGTACCGCATGCATGAGGGCATCTGTGCCTTCCC
CTCTGTGGCGTTCTACAAGAGCAAGCTGAAGACGTGGCAGGGCCTGAGGAGGCCGCCCAG
TGTCCTGGGCCACGCTGGCAAGGAGAGCTGTCCTGTCATCTTTGGCCACGTGCAGGGCCA
CGAGCGGAGCCTGCTGGTGTCCACGGACGAAGGGAATGAGAACTCCAAGGCCAACCTGGA
GGAGGTGGCTGAGGTGGTCCGTATCACCAAGCAGCTGACCCTGGGGAGGACCGTAGAGCC
CCAGGACATCGCCGTCCTCACGCCCTACAACGCGCAGGCCTCTGAGATCAGCAAGGCCCT
TCGGCGAGAGGGCATCGCCGGGGTGGCCGTGTCCTCCATCACCAAGAGCCAGGGGAGCGA
GTGGCGCTATGTGCTGGTGAGCACCGTCCGCACCTGTGCCAAGAGCGACCTGGACCAGCG
GCCCACCAAGAGCTGGCTCAAGAAGTTTCTGGGCTTCGTTGTGGACCCCAACCAAGTGAA
CGTGGCTGTCACGCGGGCCCAGGAGGGGCTCTGCCTGATCGGAGACCACCTCCTTCTGCG
CTGCTGCCCCCTCTGGCGTAGCCTCCTGGACTTCTGCGAGGCTCAGCAGACCCTCGTGCC
TGCCGGCCAGGTGCGCGTCTGCAGGAGGCCAACTATGCCTTCCTACGTAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT