NCEH1 ORF clone pFN21ASDA1363

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Halotag ORF Clone pFN21ASDA1363

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Clone info.

Species

human

Clone Name

pFN21ASDA1363 Sequence (with vector) Restriction Map

Product ID

FHC00815

Flexi Clone PID

FXC00815

ORF Sequence

DNA sequence (1224 bp)
Protein sequence (408 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384187]

Gene

KIAA1363

Symbol

NCEH1

Alias : AADACL1, NCEH

Description

neutral cholesterol ester hydrolase 1

Original Clone ID

fj02385

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1224 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 408 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33893.1 1.1e-185 99.8 NCEH1 CCDS54682.1 1.8e-183 97.6 NCEH1 CCDS54681.1 2.8e-123 100.0 NCEH1 CCDS33877.1 2.6e-74 43.2 AADAC CCDS3161.2 3e-63 38.7 AADACL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013094 109 262 PF07859 Alpha/beta hydrolase fold-3 IPR013094 321 381 PF07859 Alpha/beta hydrolase fold-3
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065843 440 613234 99.8 100.0 NP_001139748 448 613234 97.6 100.0 NP_001077 399 600338 43.2 99.8 XP_005247161 382 600338 42.3 99.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1363 Download

>KIAA1363  1224 bp
ATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCTGGCCACCTATTACGTCTAC
ATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGACGCCACTTTC
CGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTG
GCACTGAATTTTATCATTGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTG
AAGGTGACCGACACAGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAG
CCCGAAGAGCCACTGAAACGCAGCGTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCA
AGTGCAAAAATCAGGTATTATGATGAGCTGTGTACAGCAATGGCTGAGGAATTGAATGCT
GTCATTGTTTCCATTGAATACAGGCTAGTTCCAAAGGTTTATTTTCCTGAGCAAATTCAT
GATGTTGTACGGGCCACAAAGTATTTCCTGAAGCCAGAAGTCTTACAGAAGTATATGGTT
GATCCAGGCAGAATTTGCATTTCTGGTGACAGTGCTGGTGGAAATCTGGCTGCTGCCCTT
GGACAACAGTTTACTCAAGATGCCAGCCTAAAAAATAAGCTCAAACTACAAGCTTTAATT
TATCCAGTTCTTCAAGCTTTAGATTTTAACACACCATCTTATCAGCAAAATGTGAACACC
CCAATCCTGCCCCGCTATGTCATGGTGAAGTATTGGGTGGACTACTTCAAAGGCAACTAT
GACTTTGTGCAGGCAATGATCGTTAACAATCACACTTCACTTGATGTGGAAGAGGCTGCT
GCTGTCAGGGCCCGTCTAAACTGGACATCCCTCTTGCCTGCATCCTTCACAAAGAACTAC
AAGCCTGTTGTACAGACCACAGGCAATGCCAGGATTGTCCAGGAGCTTCCTCAGTTGCTG
GATGCCCGCTCCGCCCCACTCATTGCAGACCAGGCAGTGCTGCAGCTCCTCCCAAAGACC
TACATTCTGACGTGTGAGCATGATGTCCTCAGAGACGATGGCATCATGTATGCCAAGCGT
TTGGAGAGTGCCGGTGTGGAGGTGACCCTGGATCACTTTGAGGATGGCTTTCACGGATGT
ATGATTTTCACTAGCTGGCCCACCAACTTCTCAGTGGGAATCCGGACTAGGAATAGTTAC
ATCAAGTGGCTAGATCAAAACCTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1363 Download

>KIAA1363  408 aa
MRSSCVLLTALVALATYYVYIPLPGSVSDPWKLMLLDATFRGAQQVSNLIHYLGLSHHLL
ALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRSVVYIHGGGWALA
SAKIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFLKPEVLQKYMV
DPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNT
PILPRYVMVKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNY
KPVVQTTGNARIVQELPQLLDARSAPLIADQAVLQLLPKTYILTCEHDVLRDDGIMYAKR
LESAGVEVTLDHFEDGFHGCMIFTSWPTNFSVGIRTRNSYIKWLDQNL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1363 Download

>pFN21ASDA1363  5953 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGAGGTCGTCCTGTGT
CCTGCTCACCGCCCTGGTGGCGCTGGCCACCTATTACGTCTACATCCCGCTGCCTGGCTC
CGTGTCCGACCCCTGGAAGCTGATGCTGCTGGACGCCACTTTCCGGGGTGCACAGCAAGT
GAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTGGCACTGAATTTTATCAT
TGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTGAAGGTGACCGACACAGA
CTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAGCCCGAAGAGCCACTGAA
ACGCAGCGTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCAAGTGCAAAAATCAGGTA
TTATGATGAGCTGTGTACAGCAATGGCTGAGGAATTGAATGCTGTCATTGTTTCCATTGA
ATACAGGCTAGTTCCAAAGGTTTATTTTCCTGAGCAAATTCATGATGTTGTACGGGCCAC
AAAGTATTTCCTGAAGCCAGAAGTCTTACAGAAGTATATGGTTGATCCAGGCAGAATTTG
CATTTCTGGTGACAGTGCTGGTGGAAATCTGGCTGCTGCCCTTGGACAACAGTTTACTCA
AGATGCCAGCCTAAAAAATAAGCTCAAACTACAAGCTTTAATTTATCCAGTTCTTCAAGC
TTTAGATTTTAACACACCATCTTATCAGCAAAATGTGAACACCCCAATCCTGCCCCGCTA
TGTCATGGTGAAGTATTGGGTGGACTACTTCAAAGGCAACTATGACTTTGTGCAGGCAAT
GATCGTTAACAATCACACTTCACTTGATGTGGAAGAGGCTGCTGCTGTCAGGGCCCGTCT
AAACTGGACATCCCTCTTGCCTGCATCCTTCACAAAGAACTACAAGCCTGTTGTACAGAC
CACAGGCAATGCCAGGATTGTCCAGGAGCTTCCTCAGTTGCTGGATGCCCGCTCCGCCCC
ACTCATTGCAGACCAGGCAGTGCTGCAGCTCCTCCCAAAGACCTACATTCTGACGTGTGA
GCATGATGTCCTCAGAGACGATGGCATCATGTATGCCAAGCGTTTGGAGAGTGCCGGTGT
GGAGGTGACCCTGGATCACTTTGAGGATGGCTTTCACGGATGTATGATTTTCACTAGCTG
GCCCACCAACTTCTCAGTGGGAATCCGGACTAGGAATAGTTACATCAAGTGGCTAGATCA
AAACCTGTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT