RUFY3 ORF clone pFN21ASDA0871

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Halotag ORF Clone pFN21ASDA0871

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Clone info.

Species

human

Clone Name

pFN21ASDA0871 Sequence (with vector) Restriction Map

Product ID

FHC00666

Flexi Clone PID

FXC00666

ORF Sequence

DNA sequence (1407 bp)
Protein sequence (469 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384025]

Gene

KIAA0871

Symbol

RUFY3

Alias : RIPX, SINGAR1, ZFYVE30

Description

RUN and FYVE domain containing 3, transcript variant 2

Original Clone ID

hk06991

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1407 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 469 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3547.1 6.3e-96 100.0 RUFY3 CCDS34001.1 8.5e-91 100.0 RUFY3 CCDS75138.1 2.9e-77 100.0 RUFY3 CCDS47068.1 8e-77 100.0 RUFY3 CCDS81470.1 2.7e-38 63.1 RUFY2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004012 103 225 PF02759 RUN HMMSmart IPR004012 163 225 SM00593 RUN ProfileScan IPR004012 95 227 PS50826 RUN
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055776 469 611194 100.0 100.0 NP_001032519 620 611194 100.0 94.9 NP_001332769 416 611194 100.0 87.6 NP_001278923 487 611194 96.3 100.0 XP_011530057 529 611194 100.0 87.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0871 Download

>KIAA0871  1407 bp
ATGTCTGCTCTGACGCCTCCGACCGATATGCCAACCCCCACCACTGACAAGATCACACAG
GCTGCCATGGAGACCATCTACCTTTGCAAATTCCGAGTGTCCATGGATGGAGAATGGCTC
TGCCTGCGAGAGCTGGATGACATCTCACTTACACCTGACCCAGAGCCTACCCATGAAGAT
CCTAATTATCTCATGGCTAATGAACGCATGAACCTCATGAACATGGCCAAGCTGAGTATC
AAGGGCTTGATTGAATCAGCTCTGAACCTGGGGAGGACTCTTGACTCTGACTATGCACCT
CTCCAGCAATTCTTTGTGGTGATGGAGCACTGTCTGAAACATGGCTTGAAAGCTAAAAAA
ACTTTTCTCGGACAAAATAAATCCTTCTGGGGGCCTCTAGAACTGGTAGAAAAGCTTGTT
CCAGAAGCCGCAGAGATAACAGCAAGTGTTAAAGATCTTCCAGGACTTAAGACACCAGTA
GGTAGAGGAAGAGCCTGGCTTCGTTTGGCATTAATGCAAAAGAAACTTTCAGAATATATG
AAAGCTTTGATCAATAAGAAAGAACTTCTCAGTGAATTCTACGAACCCAATGCCCTCATG
ATGGAAGAAGAAGGAGCCATAATTGCTGGTCTGTTGGTGGGTCTGAATGTCATTGATGCC
AATTTCTGTATGAAAGGAGAAGACTTGGACTCTCAGGTTGGAGTTATAGATTTTTCAATG
TATCTCAAGGACGGGAACAGCAGTAAAGGTACTGAAGGAGACGGTCAGATTACTGCAATT
CTGGACCAGAAGAACTATGTAGAAGAACTGAACAGACATTTGAATGCTACTGTAAACAAC
CTTCAGGCAAAAGTAGATGCATTAGAAAAATCCAACACTAAACTGACAGAGGAGCTTGCA
GTTGCAAACAACAGGATCATTACCTTACAAGAAGAAATGGAACGAGTTAAAGAGGAAAGT
TCCTACATACTGGAATCCAATCGGAAGGGTCCCAAGCAAGACAGAACTGCAGAAGGGCAA
GCACTAAGTGAAGCAAGAAAGCATTTAAAAGAAGAGACACAATTACGATTGGATGTTGAG
AAAGAACTGGAGATGCAGATCAGCATGAGGCAGGAGATGGAATTGGCTATGAAGATGCTG
GAGAAGGATGTCTGTGAGAAGCAGGATGCCCTGGTATCTCTTCGGCAGCAGCTGGATGAC
CTCAGAGCTCTCAAGCATGAACTTGCCTTTAAGCTGCAGAGTTCAGACTTAGGAGTAAAA
CAGAAAAGTGAACTAAACAGTCGCTTGGAAGAGAAGACTAATCAGATGGCTGCTACCATT
AAACAACTTGAACAAAGTGAAAAGGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCA
GCAAATAAACTGATCCCAAAACATCAT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0871 Download

>KIAA0871  469 aa
MSALTPPTDMPTPTTDKITQAAMETIYLCKFRVSMDGEWLCLRELDDISLTPDPEPTHED
PNYLMANERMNLMNMAKLSIKGLIESALNLGRTLDSDYAPLQQFFVVMEHCLKHGLKAKK
TFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYM
KALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSM
YLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELA
VANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVE
KELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVK
QKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0871 Download

>pFN21ASDA0871  6136 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGTCTGCTCTGACGCC
TCCGACCGATATGCCAACCCCCACCACTGACAAGATCACACAGGCTGCCATGGAGACCAT
CTACCTTTGCAAATTCCGAGTGTCCATGGATGGAGAATGGCTCTGCCTGCGAGAGCTGGA
TGACATCTCACTTACACCTGACCCAGAGCCTACCCATGAAGATCCTAATTATCTCATGGC
TAATGAACGCATGAACCTCATGAACATGGCCAAGCTGAGTATCAAGGGCTTGATTGAATC
AGCTCTGAACCTGGGGAGGACTCTTGACTCTGACTATGCACCTCTCCAGCAATTCTTTGT
GGTGATGGAGCACTGTCTGAAACATGGCTTGAAAGCTAAAAAAACTTTTCTCGGACAAAA
TAAATCCTTCTGGGGGCCTCTAGAACTGGTAGAAAAGCTTGTTCCAGAAGCCGCAGAGAT
AACAGCAAGTGTTAAAGATCTTCCAGGACTTAAGACACCAGTAGGTAGAGGAAGAGCCTG
GCTTCGTTTGGCATTAATGCAAAAGAAACTTTCAGAATATATGAAAGCTTTGATCAATAA
GAAAGAACTTCTCAGTGAATTCTACGAACCCAATGCCCTCATGATGGAAGAAGAAGGAGC
CATAATTGCTGGTCTGTTGGTGGGTCTGAATGTCATTGATGCCAATTTCTGTATGAAAGG
AGAAGACTTGGACTCTCAGGTTGGAGTTATAGATTTTTCAATGTATCTCAAGGACGGGAA
CAGCAGTAAAGGTACTGAAGGAGACGGTCAGATTACTGCAATTCTGGACCAGAAGAACTA
TGTAGAAGAACTGAACAGACATTTGAATGCTACTGTAAACAACCTTCAGGCAAAAGTAGA
TGCATTAGAAAAATCCAACACTAAACTGACAGAGGAGCTTGCAGTTGCAAACAACAGGAT
CATTACCTTACAAGAAGAAATGGAACGAGTTAAAGAGGAAAGTTCCTACATACTGGAATC
CAATCGGAAGGGTCCCAAGCAAGACAGAACTGCAGAAGGGCAAGCACTAAGTGAAGCAAG
AAAGCATTTAAAAGAAGAGACACAATTACGATTGGATGTTGAGAAAGAACTGGAGATGCA
GATCAGCATGAGGCAGGAGATGGAATTGGCTATGAAGATGCTGGAGAAGGATGTCTGTGA
GAAGCAGGATGCCCTGGTATCTCTTCGGCAGCAGCTGGATGACCTCAGAGCTCTCAAGCA
TGAACTTGCCTTTAAGCTGCAGAGTTCAGACTTAGGAGTAAAACAGAAAAGTGAACTAAA
CAGTCGCTTGGAAGAGAAGACTAATCAGATGGCTGCTACCATTAAACAACTTGAACAAAG
TGAAAAGGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCAGCAAATAAACTGATCCC
AAAACATCATTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT