RAPGEF5 ORF clone pFN21ASDA0277

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA0277

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Clone info.

Species

human

Clone Name

pFN21ASDA0277 Sequence (with vector) Restriction Map

Product ID

FHC00490

Flexi Clone PID

FXC00490

ORF Sequence

DNA sequence (1740 bp)
Protein sequence (580 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383833]

Gene

KIAA0277

Symbol

RAPGEF5

Alias : GFR, MR-GEF, MRGEF, REPAC

Description

Rap guanine nucleotide exchange factor (GEF) 5

Original Clone ID

ha06833

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1740 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 580 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55093.1 3.5e-207 95.4 RAPGEF5 CCDS74604.1 3.9e-94 47.2 RAPGEF4 CCDS63061.1 4.1e-94 47.2 RAPGEF4 CCDS63060.1 4.2e-94 47.2 RAPGEF4 CCDS42776.1 4.2e-94 47.2 RAPGEF4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000651 72 173 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 344 525 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR000651 67 201 SM00229 Ras-like guanine nucleotide exchange factor IPR001895 341 580 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR000651 68 201 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 345 579 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011513954 554 609527 95.4 93.3 XP_005249973 730 609527 95.4 93.3 NP_036426 730 609527 95.4 93.3 XP_016868326 758 609527 95.4 93.3 XP_005249971 883 609527 95.4 93.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0277 Download

>KIAA0277  1740 bp
ATGGGCAGCTCCCGGCTGAGGGTCTTTGACCCTCATTTGGAGAGGAAAGATTCCGCCGCG
GCGCTCTCAGACCGAGAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATC
GACGAGGAGGATGAGGACGATGAATGGAGCAGCCGTTCGCAGTCTTCCACCGAGGATGAC
TCAGTGGACTCTCTGCTCTCTGACAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATT
TTGGAGCACCTTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACC
CTCCTGGATGACTTCCTTCTCACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAG
GCTCTGTTAAGGCACTATTCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCAGATGTT
CCGCGTAGGAAACGTAAAGTCTTGCATCTTGTTTCCCAGTGGATTGCTCTGTACAAAGAC
TGGTTACCTGAAGATGAACATTCAAAAATGTTTTTAAAGACCATATATAGGAATGTACTG
GATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAGATACTT
GGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAAGCCCTT
TTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACG
GAGGAAATTTTCTGCCACGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAA
GTTTCCAGTATAGCCCAAGAGATCCTAAAAGTCGTGGCAGAAAAGATCCAGTATGCAGAA
GAGGATCTGGCTCTGGTGGCCATCACATTCTCTGGGGAAAAGCATGAACTTCAGCCAAAT
GACTTAGTCATCTCCAAATCCCTCGAGGCATCTGGTCGAATATATGTCTACCGGAAAGAC
CTGGCGGACACTTTGAACCCATTTGCAGAAAATGAGGAATCACAGCAAAGGTCGATGAGG
ATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGGAGTCTA
TTCAATTCAATTCACGAGCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGTGGG
GAACACACTGCAAATCTCAGCCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTTTGGGTG
GCCACGGAGATTCTGCTCTGCAGCCAGCTGGGCAAGCGAGTGCAGCTGGTGAAAAAATTC
ATCAAAATTGCGGCTCACTGCAAAGCCCAGAGAAACCTGAATTCTTTCTTTGCCATTGTG
ATGGGTCTCAACACTGCTTCTGTCAGTCGACTGTCGCAGACCTGGGAGAAAATCCCTGGG
AAGTTTAAGAAACTTTTCTCTGAACTTGAAAGTTTAACAGATCCTTCCCTAAATCACAAA
GCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATCCCTTTCATGCCCTTATTG
CTTAAAGATGTAACATTTATTCATGAAGGAAATAAAACTTTTTTGGATAATCTTGTCAAT
TTTGAAAAGCTGCATATGATCGCAGACACTGTCCGAACCCTGAGACACTGCAGGACTAAC
CAGTTTGGTGACCTGTCTCCAAAAGAGCATCAAGAGTTAAAGTCCTATGTTAATCACCTG
TATGTCATTGACAGCCAGCAGGCTCTGTTTGAGCTCTCACACAGGATCGAGCCTCGGGTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0277 Download

>KIAA0277  580 aa
MGSSRLRVFDPHLERKDSAAALSDRELPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDD
SVDSLLSDRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQ
ALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVL
DDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTET
EEIFCHVYITEHSYVSVKAKVSSIAQEILKVVAEKIQYAEEDLALVAITFSGEKHELQPN
DLVISKSLEASGRIYVYRKDLADTLNPFAENEESQQRSMRILGMNTWDLALELMNFDWSL
FNSIHEQELIYFTFSRQGSGEHTANLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKF
IKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHK
AYRDAFKKMKPPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTN
QFGDLSPKEHQELKSYVNHLYVIDSQQALFELSHRIEPRV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0277 Download

>pFN21ASDA0277  6469 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGGCAGCTCCCGGCT
GAGGGTCTTTGACCCTCATTTGGAGAGGAAAGATTCCGCCGCGGCGCTCTCAGACCGAGA
GCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATCGACGAGGAGGATGAGGA
CGATGAATGGAGCAGCCGTTCGCAGTCTTCCACCGAGGATGACTCAGTGGACTCTCTGCT
CTCTGACAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATTTTGGAGCACCTTTTGAA
TGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACCCTCCTGGATGACTTCCT
TCTCACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAGGCTCTGTTAAGGCACTA
TTCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCAGATGTTCCGCGTAGGAAACGTAA
AGTCTTGCATCTTGTTTCCCAGTGGATTGCTCTGTACAAAGACTGGTTACCTGAAGATGA
ACATTCAAAAATGTTTTTAAAGACCATATATAGGAATGTACTGGATGATGTTTATGAATA
TCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAGATACTTGGAATGCACCGTCGTCA
CACTGTAGATGAATATTCACCACAAAAAAAGAATAAAGCCCTTTTCCACCAATTCAGTCT
TAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACGGAGGAAATTTTCTGCCA
CGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAAGTTTCCAGTATAGCCCA
AGAGATCCTAAAAGTCGTGGCAGAAAAGATCCAGTATGCAGAAGAGGATCTGGCTCTGGT
GGCCATCACATTCTCTGGGGAAAAGCATGAACTTCAGCCAAATGACTTAGTCATCTCCAA
ATCCCTCGAGGCATCTGGTCGAATATATGTCTACCGGAAAGACCTGGCGGACACTTTGAA
CCCATTTGCAGAAAATGAGGAATCACAGCAAAGGTCGATGAGGATTTTGGGAATGAACAC
TTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGGAGTCTATTCAATTCAATTCACGA
GCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGTGGGGAACACACTGCAAATCT
CAGCCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTTTGGGTGGCCACGGAGATTCTGCT
CTGCAGCCAGCTGGGCAAGCGAGTGCAGCTGGTGAAAAAATTCATCAAAATTGCGGCTCA
CTGCAAAGCCCAGAGAAACCTGAATTCTTTCTTTGCCATTGTGATGGGTCTCAACACTGC
TTCTGTCAGTCGACTGTCGCAGACCTGGGAGAAAATCCCTGGGAAGTTTAAGAAACTTTT
CTCTGAACTTGAAAGTTTAACAGATCCTTCCCTAAATCACAAAGCCTACAGAGATGCATT
CAAAAAGATGAAGCCACCAAAAATCCCTTTCATGCCCTTATTGCTTAAAGATGTAACATT
TATTCATGAAGGAAATAAAACTTTTTTGGATAATCTTGTCAATTTTGAAAAGCTGCATAT
GATCGCAGACACTGTCCGAACCCTGAGACACTGCAGGACTAACCAGTTTGGTGACCTGTC
TCCAAAAGAGCATCAAGAGTTAAAGTCCTATGTTAATCACCTGTATGTCATTGACAGCCA
GCAGGCTCTGTTTGAGCTCTCACACAGGATCGAGCCTCGGGTGTACGTAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT