EDEM1 ORF clone pFN21ASDA0212

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Halotag ORF Clone pFN21ASDA0212

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Clone info.

Species

human

Clone Name

pFN21ASDA0212 Sequence (with vector) Restriction Map

Product ID

FHC00461

Flexi Clone PID

FXC00461

ORF Sequence

DNA sequence (1971 bp)
Protein sequence (657 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383802]

Gene

KIAA0212

Symbol

EDEM1

Alias : EDEM

Description

ER degradation enhancer, mannosidase alpha-like 1

Original Clone ID

ha02602

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1971 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 657 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33686.1 9e-180 100.0 EDEM1 CCDS1363.2 1.1e-28 44.8 EDEM3 CCDS46592.1 1.5e-14 41.2 EDEM2 CCDS13247.1 1.5e-14 40.8 EDEM2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001382 137 157 PR00747 Glycoside hydrolase IPR001382 183 197 PR00747 Glycoside hydrolase IPR001382 220 238 PR00747 Glycoside hydrolase IPR001382 274 293 PR00747 Glycoside hydrolase IPR001382 366 383 PR00747 Glycoside hydrolase IPR001382 434 450 PR00747 Glycoside hydrolase IPR001382 489 513 PR00747 Glycoside hydrolase IPR001382 548 568 PR00747 Glycoside hydrolase HMMPfam IPR001382 137 584 PF01532 Glycoside hydrolase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 VLGLVLLRLGLHGVLWLVFGLGP 29 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055489 657 607673 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0212 Download

>KIAA0212  1971 bp
ATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTA
TTGTGGCTCGTCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGC
TTCGGCTTCCAGCGTCTGAGGAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTG
GGCCGGCCTGGGGGGGTATCCGGGCCGTCGTGGCTGCAGCCGCCGGGGACCGGGGCAGCG
CAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGATGTGCGGCCCAGCCAACTGG
GGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCGCTACAGCGGC
GCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTT
GGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGC
CGCGGCCGTGGGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGG
AACTACTCATTGACTCTTGTTGATGCATTGGATACACTTGCAATAATGGGAAATTCATCC
GAGTTCCAGAAAGCAGTCAAGTTAGTGATCAACACAGTTTCATTTGACAAAGATTCCACC
GTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCCTCCTTTCTGCTCACAGAATA
ATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGATAATGAGTTG
TTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACA
GGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAG
ACATGCACAGCGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTG
GGGGACTCCACATTTGAGTGGGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGG
AGCAATGATACAGGATTACTAGGCAATGTCGTGAACATTCAGACGGGCCACTGGGTTGGA
AAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATGAATACCTCTTGAAATCTTAC
ATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCAGAGTATTCAG
AACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTAT
GTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCC
TTTTTCCCTGGACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCC
TTCTACTATGCCATATGGAAACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTG
CAGGCCCCTGACGTTCTCTTCTACCCACTGAGACCAGAGTTAGTGGAATCCACATATCTC
CTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGATATTCTGCAGAGT
CTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGTCATTGACAAG
TCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTG
CTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAG
GGACACATTGTATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCT
GAAGAGGGAGGGCAGGACCAAGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTA
AAAGTCATCAACTCCAGCTCCAACTGCAATCGTGTACCTGATGAGAGGAGGTACTCCCTG
CCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGGTTGGTTTGATT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0212 Download

>KIAA0212  657 aa
MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPV
GRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSG
AFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLG
NYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRI
ITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNE
TCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVG
KQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLY
VNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQL
QAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDK
STEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFS
EEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0212 Download

>pFN21ASDA0212  6700 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCAATGGCGAGCGCT
CGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTATTGTGGCTCGTCTTCGG
GCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGCTTCGGCTTCCAGCGTCT
GAGGAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTGGGCCGGCCTGGGGGGGT
ATCCGGGCCGTCGTGGCTGCAGCCGCCGGGGACCGGGGCAGCGCAGAGCCCGCGCAAGGC
TCCGCGGCGTCCTGGGCCGGGGATGTGCGGCCCAGCCAACTGGGGCTACGTGCTGGGCGG
CCGGGGCCGCGGCCCGGACGAGTACGAGAAGCGCTACAGCGGCGCCTTCCCTCCGCAGCT
GCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTTGGCTACGACAACTACAT
GGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGCCGCGGCCGTGGGCCCGA
CCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGGAACTACTCATTGACTCT
TGTTGATGCATTGGATACACTTGCAATAATGGGAAATTCATCCGAGTTCCAGAAAGCAGT
CAAGTTAGTGATCAACACAGTTTCATTTGACAAAGATTCCACCGTCCAAGTCTTTGAGGC
CACGATAAGGGTCCTGGGAAGCCTCCTTTCTGCTCACAGAATAATAACTGACTCCAAGCA
GCCCTTTGGTGACATGACAATTAAGGACTATGATAATGAGTTGTTATACATGGCCCATGA
CCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACAGGGATTCCATATCCTCG
GGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAGACATGCACAGCGGGAGC
CGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTGGGGGACTCCACATTTGA
GTGGGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGGAGCAATGATACAGGATT
ACTAGGCAATGTCGTGAACATTCAGACGGGCCACTGGGTTGGAAAGCAGAGTGGCCTGGG
TGCCGGGCTGGACTCCTTCTATGAATACCTCTTGAAATCTTACATTCTCTTTGGAGAAAA
AGAAGACCTAGAAATGTTTAATGCTGCATATCAGAGTATTCAGAACTACTTAAGAAGAGG
GCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTATGTCAACGTGAACATGTT
CAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCCTTTTTCCCTGGACTGCA
GGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCCTTCTACTATGCCATATG
GAAACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTGCAGGCCCCTGACGTTCT
CTTCTACCCACTGAGACCAGAGTTAGTGGAATCCACATATCTCCTCTACCAGGCAACCAA
GAATCCCTTCTACCTCCATGTAGGAATGGATATTCTGCAGAGTCTGGAAAAGTACACAAA
AGTCAAGTGTGGGTACGCCACGCTGCATCACGTCATTGACAAGTCCACAGAAGACCGGAT
GGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTGCTGTTTGATGAAGACAA
TCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAGGGACACATTGTATCTGT
GGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCTGAAGAGGGAGGGCAGGA
CCAAGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTAAAAGTCATCAACTCCAG
CTCCAACTGCAATCGTGTACCTGATGAGAGGAGGTACTCCCTGCCCTTAAAGAGCATCTA
CATGCGACAGATTGACCAGATGGTTGGTTTGATTTACGTAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT