GCNT2 ORF clone pFN21AF0405

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AF0405

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Clone info.

Species

human

Clone Name

pFN21AF0405 Sequence (with vector) Restriction Map

Product ID

FHC01655

Flexi Clone PID

FXC01655

ORF Sequence

DNA sequence (1206 bp)
Protein sequence (402 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385263]

Gene

FLJ00405

Symbol

GCNT2

Alias : CCAT, CTRCT13, GCNT2C, GCNT5, IGNT, II, NACGT1, NAGCT1, ULG3, bA360O19.2, bA421M1.1

Description

glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group), transcript variant 1

Original Clone ID

sj08107

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1206 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 402 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34338.1 9.9e-193 100.0 GCNT2 CCDS4513.1 9.1e-142 73.1 GCNT2 CCDS4512.1 2.2e-139 73.9 GCNT2 CCDS10172.1 8.8e-65 43.7 GCNT3 CCDS4026.1 1.1e-63 43.7 GCNT4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003406 97 357 PF02485 Glycosyl transferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 WKHCLFSASLISALIFVFVYNT 26 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_663624 402 110800,116700,600429 100.0 100.0 XP_006715115 402 110800,116700,600429 100.0 100.0 XP_005249056 325 110800,116700,600429 100.0 80.8 NP_663630 402 110800,116700,600429 73.1 99.8 NP_001482 400 110800,116700,600429 73.9 98.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AF0405 Download

>FLJ00405  1206 bp
ATGATGGGCTCTTGGAAGCACTGTCTTTTTAGCGCGTCTCTTATCTCTGCCCTGATTTTT
GTATTTGTTTACAATACTGAGTTATGGGAGAATAAACGTTTTCTGAGGGCAGCTCTGTCC
AATGCTTCACTGTTAGCAGAAGCCTGTCATCAGATTTTTGAGGGGAAAGTTTTTTACCCA
ACAGAAAATGCATTGAAAACTACCCTTGATGAAGCTACCTGCTATGAGTACATGGTTCGA
AGCCACTATGTAACAGAAACACTCTCTGAAGAAGAGGCTGGGTTCCCTTTAGCTTACACA
GTGACCATCCACAAAGACTTCGGCACTTTTGAGAGGCTCTTCAGGGCGATTTATATGCCC
CAAAATGTCTACTGTGTGCACCTGGATCAGAAGGCGACGGATGCCTTTAAAGGTGCAGTG
AAACAGTTACTCAGCTGCTTCCCAAATGCTTTTCTGGCTTCCAAGAAGGAGTCGGTTGTC
TATGGGGGGATCTCCAGGCTCCAGGCTGACCTGAACTGCCTGGAAGACCTTGTGGCCTCT
GAAGTTCCCTGGAAGTATGTCATCAACACCTGCGGGCAAGACTTTCCCCTGAAAACCAAC
AGGGAAATAGTTCAGTATCTGAAGGGATTTAAAGGGAAAAATATCACCCCCGGAGTGCTG
CCTCCTGACCACGCTGTTGGACGGACTAAATACGTCCACCAAGAACTGTTAAACCACAAA
AATTCCTACGTGATTAAAACAACAAAATTAAAAACTCCTCCTCCTCATGACATGGTGATT
TACTTTGGCACGGCCTACGTGGCTCTCACAAGGGACTTTGCTAACTTCGTCCTCCAAGAC
CAGCTCGCACTTGACTTACTCTCCTGGTCCAAGGACACCTACAGCCCCGACGAACATTTC
TGGGTGACACTCAACAGGATTCCCGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACT
GGAAACCTCAGAGCTATAAAGTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGC
CACTATGTACATGGTATTTGTATCTATGGAAACGGAGACTTAAAGTGGCTGGTTAATTCA
CCAAGCCTGTTTGCTAACAAGTTTGAGCTTAATACCTACCCCCTTACTGTGGAATGCCTA
GAACTGAGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACAACCCAGCTGG
TATTTT

ORF Protein

Cloned ORF protein sequence for pFN21AF0405 Download

>FLJ00405  402 aa
MMGSWKHCLFSASLISALIFVFVYNTELWENKRFLRAALSNASLLAEACHQIFEGKVFYP
TENALKTTLDEATCYEYMVRSHYVTETLSEEEAGFPLAYTVTIHKDFGTFERLFRAIYMP
QNVYCVHLDQKATDAFKGAVKQLLSCFPNAFLASKKESVVYGGISRLQADLNCLEDLVAS
EVPWKYVINTCGQDFPLKTNREIVQYLKGFKGKNITPGVLPPDHAVGRTKYVHQELLNHK
NSYVIKTTKLKTPPPHDMVIYFGTAYVALTRDFANFVLQDQLALDLLSWSKDTYSPDEHF
WVTLNRIPGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNS
PSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AF0405 Download

>pFN21AF0405  5911 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGGGCTCTTGGAAGCACTGTCTTTTTAGCGC
GTCTCTTATCTCTGCCCTGATTTTTGTATTTGTTTACAATACTGAGTTATGGGAGAATAA
ACGTTTTCTGAGGGCAGCTCTGTCCAATGCTTCACTGTTAGCAGAAGCCTGTCATCAGAT
TTTTGAGGGGAAAGTTTTTTACCCAACAGAAAATGCATTGAAAACTACCCTTGATGAAGC
TACCTGCTATGAGTACATGGTTCGAAGCCACTATGTAACAGAAACACTCTCTGAAGAAGA
GGCTGGGTTCCCTTTAGCTTACACAGTGACCATCCACAAAGACTTCGGCACTTTTGAGAG
GCTCTTCAGGGCGATTTATATGCCCCAAAATGTCTACTGTGTGCACCTGGATCAGAAGGC
GACGGATGCCTTTAAAGGTGCAGTGAAACAGTTACTCAGCTGCTTCCCAAATGCTTTTCT
GGCTTCCAAGAAGGAGTCGGTTGTCTATGGGGGGATCTCCAGGCTCCAGGCTGACCTGAA
CTGCCTGGAAGACCTTGTGGCCTCTGAAGTTCCCTGGAAGTATGTCATCAACACCTGCGG
GCAAGACTTTCCCCTGAAAACCAACAGGGAAATAGTTCAGTATCTGAAGGGATTTAAAGG
GAAAAATATCACCCCCGGAGTGCTGCCTCCTGACCACGCTGTTGGACGGACTAAATACGT
CCACCAAGAACTGTTAAACCACAAAAATTCCTACGTGATTAAAACAACAAAATTAAAAAC
TCCTCCTCCTCATGACATGGTGATTTACTTTGGCACGGCCTACGTGGCTCTCACAAGGGA
CTTTGCTAACTTCGTCCTCCAAGACCAGCTCGCACTTGACTTACTCTCCTGGTCCAAGGA
CACCTACAGCCCCGACGAACATTTCTGGGTGACACTCAACAGGATTCCCGGTGTTCCTGG
CTCTATGCCAAATGCATCCTGGACTGGAAACCTCAGAGCTATAAAGTGGAGTGACATGGA
AGACAGACACGGAGGCTGCCACGGCCACTATGTACATGGTATTTGTATCTATGGAAACGG
AGACTTAAAGTGGCTGGTTAATTCACCAAGCCTGTTTGCTAACAAGTTTGAGCTTAATAC
CTACCCCCTTACTGTGGAATGCCTAGAACTGAGGCATCGCGAAAGAACCCTCAATCAGAG
TGAAACTGCGATACAACCCAGCTGGTATTTTGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT