NPBWR1 ORF clone pFN21AE9511

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Halotag ORF Clone pFN21AE9511

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Clone info.

Species

human

Clone Name

pFN21AE9511 Sequence (with vector) Restriction Map

Product ID

FHC23268

ORF Sequence

DNA sequence (984 bp)
Protein sequence (328 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9511

Symbol

NPBWR1

Alias : GPR7

Description

neuropeptides B/W receptor 1

Original Clone ID

cp99511

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 984 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 328 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6151.1 2.9e-96 100.0 NPBWR1 CCDS13557.1 9e-57 63.7 NPBWR2 CCDS13944.1 2.7e-33 41.2 SSTR3 CCDS329.1 4.8e-33 40.0 OPRD1 CCDS9666.1 8.5e-32 37.1 SSTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 39 63 PR00237 GPCR IPR000276 72 93 PR00237 GPCR IPR009150 83 96 PR01855 Neuropeptide W receptor IPR000276 116 138 PR00237 GPCR IPR009150 139 151 PR01855 Neuropeptide W receptor IPR000276 154 175 PR00237 GPCR IPR009150 155 167 PR01855 Neuropeptide W receptor IPR009150 170 179 PR01855 Neuropeptide W receptor IPR009150 187 196 PR01855 Neuropeptide W receptor IPR009150 199 211 PR01855 Neuropeptide W receptor IPR000276 204 227 PR00237 GPCR IPR009150 229 242 PR01855 Neuropeptide W receptor IPR000276 249 273 PR00237 GPCR IPR009150 261 275 PR01855 Neuropeptide W receptor IPR000276 289 315 PR00237 GPCR HMMPfam IPR000276 54 307 PF00001 GPCR ProfileScan IPR017452 54 307 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 36 LAVAVPVVYAVICAVGLAGNSAV 58 PRIMARY 23 2 73 NLFILNLAIADELFTLVLPINIA 95 SECONDARY 23 3 119 NTFSSLYFLTVMSADRYLVVLAT 141 SECONDARY 23 4 153 SAARAVSLAVWGIVTLVVLPFAV 175 PRIMARY 23 5 207 TLVLGFAIPVSTICVLYTTLLCR 229 PRIMARY 23 6 249 VTFLVVAILAVCLLCWTPYHLST 271 PRIMARY 23 7 273 VALTTDLPQTPLVIAISYFITSL 295 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005276 328 600730 100.0 100.0 NP_005277 333 600731 63.7 97.6 NP_001265616 418 182453 41.2 97.3 XP_006724374 418 182453 41.2 97.3 NP_001042 418 182453 41.2 97.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9511 Download

>KIEE9511  984 bp
ATGGACAACGCCTCGTTCTCGGAGCCCTGGCCCGCCAACGCATCGGGCCCGGACCCGGCG
CTGAGCTGCTCCAACGCGTCGACTCTGGCGCCGCTGCCGGCGCCGCTGGCGGTGGCTGTA
CCAGTTGTCTACGCGGTGATCTGCGCCGTGGGTCTGGCGGGCAACTCCGCCGTGCTGTAC
GTGTTGCTGCGGGCGCCCCGCATGAAGACCGTCACCAACCTGTTCATCCTCAACCTGGCC
ATCGCCGACGAGCTCTTCACGCTGGTGCTGCCCATCAACATCGCCGACTTCCTGCTGCGG
CAGTGGCCCTTCGGGGAGCTCATGTGCAAGCTCATCGTGGCTATCGACCAGTACAACACC
TTCTCCAGCCTCTACTTCCTCACCGTCATGAGCGCCGACCGCTACCTGGTGGTGTTGGCC
ACTGCGGAGTCGCGCCGGGTGGCCGGCCGCACCTACAGCGCCGCGCGCGCGGTGAGCCTG
GCCGTGTGGGGGATCGTCACACTCGTCGTGCTGCCCTTCGCAGTCTTCGCCCGGCTAGAC
GACGAGCAGGGCCGGCGCCAGTGCGTGCTAGTCTTTCCGCAGCCCGAGGCCTTCTGGTGG
CGCGCGAGCCGCCTCTACACGCTCGTGCTGGGCTTCGCCATCCCCGTGTCCACCATCTGT
GTCCTCTATACCACCCTGCTGTGCCGGCTGCATGCCATGCGGCTGGACAGCCACGCCAAG
GCCCTGGAGCGCGCCAAGAAGCGGGTGACCTTCCTGGTGGTGGCAATCCTGGCGGTGTGC
CTCCTCTGCTGGACGCCCTACCACCTGAGCACCGTGGTGGCGCTCACCACCGACCTCCCG
CAGACGCCGCTGGTCATCGCTATCTCCTACTTCATCACCAGCCTGAGCTACGCCAACAGC
TGCCTCAACCCCTTCCTCTACGCCTTCCTGGACGCCAGCTTCCGCAGGAACCTCCGCCAG
CTGATAACTTGCCGCGCGGCAGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9511 Download

>KIEE9511  328 aa
MDNASFSEPWPANASGPDPALSCSNASTLAPLPAPLAVAVPVVYAVICAVGLAGNSAVLY
VLLRAPRMKTVTNLFILNLAIADELFTLVLPINIADFLLRQWPFGELMCKLIVAIDQYNT
FSSLYFLTVMSADRYLVVLATAESRRVAGRTYSAARAVSLAVWGIVTLVVLPFAVFARLD
DEQGRRQCVLVFPQPEAFWWRASRLYTLVLGFAIPVSTICVLYTTLLCRLHAMRLDSHAK
ALERAKKRVTFLVVAILAVCLLCWTPYHLSTVVALTTDLPQTPLVIAISYFITSLSYANS
CLNPFLYAFLDASFRRNLRQLITCRAAA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9511 Download

>pFN21AE9511  5689 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACAACGCCTCGTTCTCGGAGCCCTGGCCCGC
CAACGCATCGGGCCCGGACCCGGCGCTGAGCTGCTCCAACGCGTCGACTCTGGCGCCGCT
GCCGGCGCCGCTGGCGGTGGCTGTACCAGTTGTCTACGCGGTGATCTGCGCCGTGGGTCT
GGCGGGCAACTCCGCCGTGCTGTACGTGTTGCTGCGGGCGCCCCGCATGAAGACCGTCAC
CAACCTGTTCATCCTCAACCTGGCCATCGCCGACGAGCTCTTCACGCTGGTGCTGCCCAT
CAACATCGCCGACTTCCTGCTGCGGCAGTGGCCCTTCGGGGAGCTCATGTGCAAGCTCAT
CGTGGCTATCGACCAGTACAACACCTTCTCCAGCCTCTACTTCCTCACCGTCATGAGCGC
CGACCGCTACCTGGTGGTGTTGGCCACTGCGGAGTCGCGCCGGGTGGCCGGCCGCACCTA
CAGCGCCGCGCGCGCGGTGAGCCTGGCCGTGTGGGGGATCGTCACACTCGTCGTGCTGCC
CTTCGCAGTCTTCGCCCGGCTAGACGACGAGCAGGGCCGGCGCCAGTGCGTGCTAGTCTT
TCCGCAGCCCGAGGCCTTCTGGTGGCGCGCGAGCCGCCTCTACACGCTCGTGCTGGGCTT
CGCCATCCCCGTGTCCACCATCTGTGTCCTCTATACCACCCTGCTGTGCCGGCTGCATGC
CATGCGGCTGGACAGCCACGCCAAGGCCCTGGAGCGCGCCAAGAAGCGGGTGACCTTCCT
GGTGGTGGCAATCCTGGCGGTGTGCCTCCTCTGCTGGACGCCCTACCACCTGAGCACCGT
GGTGGCGCTCACCACCGACCTCCCGCAGACGCCGCTGGTCATCGCTATCTCCTACTTCAT
CACCAGCCTGAGCTACGCCAACAGCTGCCTCAACCCCTTCCTCTACGCCTTCCTGGACGC
CAGCTTCCGCAGGAACCTCCGCCAGCTGATAACTTGCCGCGCGGCAGCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT