MRGPRX3 ORF clone pFN21AE9509

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9509

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Clone info.

Species

human

Clone Name

pFN21AE9509 Sequence (with vector) Restriction Map

Product ID

FHC24163

ORF Sequence

DNA sequence (966 bp)
Protein sequence (322 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9509

Symbol

MRGPRX3

Alias : GPCR, MRGX3, SNSR1, SNSR2

Description

MAS-related GPR, member X3

Original Clone ID

cp99509

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 966 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 322 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7830.1 2.8e-112 99.7 MRGPRX3 CCDS7846.1 2.7e-91 83.5 MRGPRX1 CCDS7831.1 4.8e-90 82.8 MRGPRX4 CCDS7847.1 9.6e-64 62.9 MRGPRX2 CCDS31625.1 8.1e-32 40.6 MRGPRD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 29 53 PR00237 GPCR IPR000276 61 82 PR00237 GPCR IPR000276 102 124 PR00237 GPCR IPR000276 138 159 PR00237 GPCR IPR000276 214 238 PR00237 GPCR IPR000276 254 280 PR00237 GPCR HMMPfam IPR000276 44 272 PF00001 GPCR ProfileScan IPR017452 44 272 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 30 FTGLTCIVSLVALTGNAVVLWLL 52 PRIMARY 23 2 62 SIYILNLVAADFLFLSGHIICSP 84 PRIMARY 23 3 95 SKILSPVMTFPYFIGLSMLSAIS 117 SECONDARY 23 4 142 MCVLLWALSLLRSILEWMFCDFL 164 PRIMARY 23 5 180 TIAWLVFLCVVLCGSSLVLLVRI 202 PRIMARY 23 6 213 RLYVTILLTVLVFLLCGLPFGIQ 235 PRIMARY 23 7 252 HVHLVSIFLSALNSSANPIIYFF 274 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011518184 322 607229 99.7 100.0 NP_473372 322 607229 99.7 100.0 NP_671732 322 607227 83.5 100.0 NP_473373 322 607230 82.8 99.4 NP_001290544 330 607228 62.9 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9509 Download

>KIEE9509  966 bp
ATGGATTCAACCATCCCAGTCTTGGGTACAGAACTGACACCAATCAACGGACGTGAGGAG
ACTCCTTGCTACAAGCAGACCCTGAGCTTCACGGGGCTGACGTGCATCGTTTCCCTTGTC
GCGCTGACAGGAAACGCGGTTGTGCTCTGGCTCCTGGGCTGCCGCATGCGCAGGAACGCT
GTCTCCATCTACATCCTCAACCTGGTCGCGGCCGACTTCCTCTTCCTTAGCGGCCACATT
ATATGTTCGCCGTTACGCCTCATCAATATCCGCCATCCCATCTCCAAAATCCTCAGTCCT
GTGATGACCTTTCCCTACTTTATAGGCCTAAGCATGCTGAGCGCCATCAGCACCGAGCGC
TGCCTGTCCATCCTGTGGCCCATCTGGTACCACTGCCGCCGCCCCAGATACCTGTCATCG
GTCATGTGTGTCCTGCTCTGGGCCCTGTCCCTGCTGCGGAGTATCCTGGAGTGGATGTTC
TGTGACTTCCTGTTTAGTGGTGCTGATTCTGTTTGGTGTGAAACGTCAGATTTCATTACA
ATCGCGTGGCTGGTTTTTTTATGTGTGGTTCTCTGTGGGTCCAGCCTGGTCCTGCTGGTC
AGGATTCTCTGTGGATCCCGGAAGATGCCGCTGACCAGGCTGTACGTGACCATCCTCCTC
ACAGTGCTGGTCTTCCTCCTCTGTGGCCTGCCCTTTGGCATTCAGTGGGCCCTGTTTTCC
AGGATCCACCTGGATTGGAAAGTCTTATTTTGTCATGTGCATCTAGTTTCCATTTTCCTG
TCCGCTCTTAACAGCAGTGCCAACCCCATCATTTACTTCTTCGTGGGCTCCTTTAGGCAG
CGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCAGAGGGCTCTGCAGGACACGCCTGAG
GTGGATGAAGGTGGAGGGTGGCTTCCTCAGGAAACCCTGGAGCTGTCGGGAAGCAGATTG
GAGCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE9509 Download

>KIEE9509  322 aa
MDSTIPVLGTELTPINGREETPCYKQTLSFTGLTCIVSLVALTGNAVVLWLLGCRMRRNA
VSIYILNLVAADFLFLSGHIICSPLRLINIRHPISKILSPVMTFPYFIGLSMLSAISTER
CLSILWPIWYHCRRPRYLSSVMCVLLWALSLLRSILEWMFCDFLFSGADSVWCETSDFIT
IAWLVFLCVVLCGSSLVLLVRILCGSRKMPLTRLYVTILLTVLVFLLCGLPFGIQWALFS
RIHLDWKVLFCHVHLVSIFLSALNSSANPIIYFFVGSFRQRQNRQNLKLVLQRALQDTPE
VDEGGGWLPQETLELSGSRLEQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9509 Download

>pFN21AE9509  5671 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATTCAACCATCCCAGTCTTGGGTACAGAACT
GACACCAATCAACGGACGTGAGGAGACTCCTTGCTACAAGCAGACCCTGAGCTTCACGGG
GCTGACGTGCATCGTTTCCCTTGTCGCGCTGACAGGAAACGCGGTTGTGCTCTGGCTCCT
GGGCTGCCGCATGCGCAGGAACGCTGTCTCCATCTACATCCTCAACCTGGTCGCGGCCGA
CTTCCTCTTCCTTAGCGGCCACATTATATGTTCGCCGTTACGCCTCATCAATATCCGCCA
TCCCATCTCCAAAATCCTCAGTCCTGTGATGACCTTTCCCTACTTTATAGGCCTAAGCAT
GCTGAGCGCCATCAGCACCGAGCGCTGCCTGTCCATCCTGTGGCCCATCTGGTACCACTG
CCGCCGCCCCAGATACCTGTCATCGGTCATGTGTGTCCTGCTCTGGGCCCTGTCCCTGCT
GCGGAGTATCCTGGAGTGGATGTTCTGTGACTTCCTGTTTAGTGGTGCTGATTCTGTTTG
GTGTGAAACGTCAGATTTCATTACAATCGCGTGGCTGGTTTTTTTATGTGTGGTTCTCTG
TGGGTCCAGCCTGGTCCTGCTGGTCAGGATTCTCTGTGGATCCCGGAAGATGCCGCTGAC
CAGGCTGTACGTGACCATCCTCCTCACAGTGCTGGTCTTCCTCCTCTGTGGCCTGCCCTT
TGGCATTCAGTGGGCCCTGTTTTCCAGGATCCACCTGGATTGGAAAGTCTTATTTTGTCA
TGTGCATCTAGTTTCCATTTTCCTGTCCGCTCTTAACAGCAGTGCCAACCCCATCATTTA
CTTCTTCGTGGGCTCCTTTAGGCAGCGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCA
GAGGGCTCTGCAGGACACGCCTGAGGTGGATGAAGGTGGAGGGTGGCTTCCTCAGGAAAC
CCTGGAGCTGTCGGGAAGCAGATTGGAGCAGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT