GPR50 ORF clone pFN21AE9435

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9435

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Clone info.

Species

human

Clone Name

pFN21AE9435 Sequence (with vector) Restriction Map

Product ID

FHC30371

ORF Sequence

DNA sequence (1851 bp)
Protein sequence (617 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9435

Symbol

GPR50

Alias : H9, Mel1c

Description

G protein-coupled receptor 50

Original Clone ID

cp99435

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1851 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 617 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44012.1 2.9e-132 99.8 GPR50 CCDS3848.1 6.5e-33 51.5 MTNR1A CCDS8290.1 2.3e-31 48.3 MTNR1B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002280 2 20 PR01151 Melatonin-related receptor type 1X IPR002280 21 33 PR01151 Melatonin-related receptor type 1X IPR000276 30 54 PR00237 GPCR IPR000276 63 84 PR00237 GPCR IPR002280 87 100 PR01151 Melatonin-related receptor type 1X IPR000276 108 130 PR00237 GPCR IPR000025 133 148 PR00857 Melatonin receptor IPR000276 144 165 PR00237 GPCR IPR000025 163 178 PR00857 Melatonin receptor IPR000025 178 196 PR00857 Melatonin receptor IPR002280 179 190 PR01151 Melatonin-related receptor type 1X IPR000276 189 212 PR00237 GPCR IPR002280 214 233 PR01151 Melatonin-related receptor type 1X IPR000025 229 243 PR00857 Melatonin receptor IPR000276 235 259 PR00237 GPCR IPR000025 254 264 PR00857 Melatonin receptor IPR000025 268 280 PR00857 Melatonin receptor IPR000276 276 302 PR00237 GPCR IPR002280 306 318 PR01151 Melatonin-related receptor type 1X IPR002280 322 337 PR01151 Melatonin-related receptor type 1X IPR002280 338 356 PR01151 Melatonin-related receptor type 1X IPR002280 357 372 PR01151 Melatonin-related receptor type 1X IPR002280 410 433 PR01151 Melatonin-related receptor type 1X IPR002280 512 528 PR01151 Melatonin-related receptor type 1X HMMPfam IPR000276 45 294 PF00001 GPCR ProfileScan IPR017452 45 294 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 28 IIFMFCAMVITIVVDLIGNSMVI 50 PRIMARY 23 2 66 FVVSLSVADMLVAIYPYPLMLHA 88 SECONDARY 23 3 102 QMVGFITGLSVVGSIFNIVAIAI 124 PRIMARY 23 4 145 TCIYLVITWIMTVLAVLPNMYIG 167 PRIMARY 23 5 193 VCIHFVLPLLIVGFCYVRIWTKV 215 PRIMARY 23 6 238 TMFVIFLLFAVCWCPINVLTVLV 260 PRIMARY 23 7 274 WLYLAAYFIAYFNSCLNAVIYGL 296 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004215 617 300207 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9435 Download

>KIEE9435  1851 bp
ATGGGGCCCACCCTAGCGGTTCCCACCCCCTATGGCTGTATTGGCTGTAAGCTACCCCAG
CCAGAATACCCACCGGCTCTAATCATCTTTATGTTCTGCGCGATGGTTATCACCATCGTT
GTAGACCTAATCGGCAACTCCATGGTCATTTTGGCTGTGACGAAGAACAAGAAGCTCCGG
AATTCTGGCAACATCTTCGTGGTCAGTCTCTCTGTGGCCGATATGCTGGTGGCCATCTAC
CCATACCCTTTGATGCTGCATGCCATGTCCATTGGGGGCTGGGATCTGAGCCAGTTACAG
TGCCAGATGGTCGGGTTCATCACAGGGCTGAGTGTGGTCGGCTCCATCTTCAACATCGTG
GCAATCGCTATCAACCGTTACTGCTACATCTGCCACAGCCTCCAGTACGAACGGATCTTC
AGTGTGCGCAATACCTGCATCTACCTGGTCATCACCTGGATCATGACCGTCCTGGCTGTC
CTGCCCAACATGTACATTGGCACCATCGAGTACGATCCTCGCACCTACACCTGCATCTTC
AACTATCTGAACAACCCTGTCTTCACTGTTACCATCGTCTGCATCCACTTCGTCCTCCCT
CTCCTCATCGTGGGTTTCTGCTACGTGAGGATCTGGACCAAAGTGCTGGCGGCCCGTGAC
CCTGCAGGGCAGAATCCTGACAACCAACTTGCTGAGGTTCGCAATTTTCTAACCATGTTT
GTGATCTTCCTCCTCTTTGCAGTGTGCTGGTGCCCTATCAACGTGCTCACTGTCTTGGTG
GCTGTCAGTCCGAAGGAGATGGCAGGCAAGATCCCCAACTGGCTTTATCTTGCAGCCTAC
TTCATAGCCTACTTCAACAGCTGCCTCAACGCTGTGATCTACGGGCTCCTCAATGAGAAT
TTCCGAAGAGAATACTGGACCATCTTCCATGCTATGCGGCACCCTATCATATTCTTCTCT
GGCCTCATCAGTGATATTCGTGAGATGCAGGAGGCCCGTACCCTGGCCCGCGCCCGTGCC
CATGCTCGCGACCAAGCTCGTGAACAAGACCGTGCCCATGCCTGTCCTGCTGTGGAGGAA
ACCCCGATGAATGTCCGGAATGTTCCATTACCTGGTGATGCTGCAGCTGGCCACCCCGAC
CGTGCCTCTGGCCACCCTAAGCCCCATTCCAGATCCTCCTCTGCCTATCGCAAATCTGCC
TCTACCCACCACAAGTCTGTCTTTAGCCACTCCAAGGCTGCCTCTGGTCACCTCAAGCCT
GTCTCTGGCCACTCCAAGCCTGCCTCTGGTCACCCCAAGTCTGCCACTGTCTACCCTAAG
CCTGCCTCTGTCCATTTCAAGGCTGACTCTGTCCATTTCAAGGGTGACTCTGTCCATTTC
AAGCCTGACTCTGTTCATTTCAAGCCTGCTTCCAGCAACCCCAAGCCCATCACTGGCCAC
CATGTCTCTGCTGGCAGCCACTCCAAGTCTGCCTTCAGTGCTGCCACCAGCCACCCTAAA
CCCACCACTGGCCACATCAAGCCAGCTACCAGCCATGCTGAGCCCACCACTGCTGACTAT
CCCAAGCCTGCCACTACCAGCCACCCTAAGCCCACTGCTGCTGACAACCCTGAGCTCTCT
GCCTCCCATTGCCCCGAGATCCCTGCCATTGCCCACCCTGTGTCTGACGACAGTGACCTC
CCTGAGTCGGCCTCTAGCCCTGCCGCTGGGCCCACCAAGCCTGCTGCCAGCCAGCTGGAG
TCTGACACCATCGCTGACCTTCCTGACCCTACTGTAGTCACTACCAGTACCAATGATTAC
CATGATGTCGTGGTTGTTGATGTTGAAGATGATCCTGATGAAATGGCTGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE9435 Download

>KIEE9435  617 aa
MGPTLAVPTPYGCIGCKLPQPEYPPALIIFMFCAMVITIVVDLIGNSMVILAVTKNKKLR
NSGNIFVVSLSVADMLVAIYPYPLMLHAMSIGGWDLSQLQCQMVGFITGLSVVGSIFNIV
AIAINRYCYICHSLQYERIFSVRNTCIYLVITWIMTVLAVLPNMYIGTIEYDPRTYTCIF
NYLNNPVFTVTIVCIHFVLPLLIVGFCYVRIWTKVLAARDPAGQNPDNQLAEVRNFLTMF
VIFLLFAVCWCPINVLTVLVAVSPKEMAGKIPNWLYLAAYFIAYFNSCLNAVIYGLLNEN
FRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEE
TPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKP
VSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGH
HVSAGSHSKSAFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPTAADNPELS
ASHCPEIPAIAHPVSDDSDLPESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDY
HDVVVVDVEDDPDEMAV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9435 Download

>pFN21AE9435  6556 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCCCACCCTAGCGGTTCCCACCCCCTATGG
CTGTATTGGCTGTAAGCTACCCCAGCCAGAATACCCACCGGCTCTAATCATCTTTATGTT
CTGCGCGATGGTTATCACCATCGTTGTAGACCTAATCGGCAACTCCATGGTCATTTTGGC
TGTGACGAAGAACAAGAAGCTCCGGAATTCTGGCAACATCTTCGTGGTCAGTCTCTCTGT
GGCCGATATGCTGGTGGCCATCTACCCATACCCTTTGATGCTGCATGCCATGTCCATTGG
GGGCTGGGATCTGAGCCAGTTACAGTGCCAGATGGTCGGGTTCATCACAGGGCTGAGTGT
GGTCGGCTCCATCTTCAACATCGTGGCAATCGCTATCAACCGTTACTGCTACATCTGCCA
CAGCCTCCAGTACGAACGGATCTTCAGTGTGCGCAATACCTGCATCTACCTGGTCATCAC
CTGGATCATGACCGTCCTGGCTGTCCTGCCCAACATGTACATTGGCACCATCGAGTACGA
TCCTCGCACCTACACCTGCATCTTCAACTATCTGAACAACCCTGTCTTCACTGTTACCAT
CGTCTGCATCCACTTCGTCCTCCCTCTCCTCATCGTGGGTTTCTGCTACGTGAGGATCTG
GACCAAAGTGCTGGCGGCCCGTGACCCTGCAGGGCAGAATCCTGACAACCAACTTGCTGA
GGTTCGCAATTTTCTAACCATGTTTGTGATCTTCCTCCTCTTTGCAGTGTGCTGGTGCCC
TATCAACGTGCTCACTGTCTTGGTGGCTGTCAGTCCGAAGGAGATGGCAGGCAAGATCCC
CAACTGGCTTTATCTTGCAGCCTACTTCATAGCCTACTTCAACAGCTGCCTCAACGCTGT
GATCTACGGGCTCCTCAATGAGAATTTCCGAAGAGAATACTGGACCATCTTCCATGCTAT
GCGGCACCCTATCATATTCTTCTCTGGCCTCATCAGTGATATTCGTGAGATGCAGGAGGC
CCGTACCCTGGCCCGCGCCCGTGCCCATGCTCGCGACCAAGCTCGTGAACAAGACCGTGC
CCATGCCTGTCCTGCTGTGGAGGAAACCCCGATGAATGTCCGGAATGTTCCATTACCTGG
TGATGCTGCAGCTGGCCACCCCGACCGTGCCTCTGGCCACCCTAAGCCCCATTCCAGATC
CTCCTCTGCCTATCGCAAATCTGCCTCTACCCACCACAAGTCTGTCTTTAGCCACTCCAA
GGCTGCCTCTGGTCACCTCAAGCCTGTCTCTGGCCACTCCAAGCCTGCCTCTGGTCACCC
CAAGTCTGCCACTGTCTACCCTAAGCCTGCCTCTGTCCATTTCAAGGCTGACTCTGTCCA
TTTCAAGGGTGACTCTGTCCATTTCAAGCCTGACTCTGTTCATTTCAAGCCTGCTTCCAG
CAACCCCAAGCCCATCACTGGCCACCATGTCTCTGCTGGCAGCCACTCCAAGTCTGCCTT
CAGTGCTGCCACCAGCCACCCTAAACCCACCACTGGCCACATCAAGCCAGCTACCAGCCA
TGCTGAGCCCACCACTGCTGACTATCCCAAGCCTGCCACTACCAGCCACCCTAAGCCCAC
TGCTGCTGACAACCCTGAGCTCTCTGCCTCCCATTGCCCCGAGATCCCTGCCATTGCCCA
CCCTGTGTCTGACGACAGTGACCTCCCTGAGTCGGCCTCTAGCCCTGCCGCTGGGCCCAC
CAAGCCTGCTGCCAGCCAGCTGGAGTCTGACACCATCGCTGACCTTCCTGACCCTACTGT
AGTCACTACCAGTACCAATGATTACCATGATGTCGTGGTTGTTGATGTTGAAGATGATCC
TGATGAAATGGCTGTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT