GPR139 ORF clone pFN21AE9429

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Halotag ORF Clone pFN21AE9429

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Clone info.

Species

human

Clone Name

pFN21AE9429 Sequence (with vector) Restriction Map

Product ID

FHC28368

ORF Sequence

DNA sequence (1059 bp)
Protein sequence (353 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9429

Symbol

GPR139

Alias : GPRg1, PGR3

Description

G protein-coupled receptor 139

Original Clone ID

cp99429

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1059 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 353 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32398.1 2e-100 100.0 GPR139 CCDS11698.1 7e-38 45.7 GPR142 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 28 52 PR00237 GPCR IPR000276 62 83 PR00237 GPCR IPR000276 108 130 PR00237 GPCR IPR000276 226 250 PR00237 GPCR IPR000276 267 293 PR00237 GPCR HMMPfam IPR000276 47 285 PF00001 GPCR ProfileScan IPR017452 43 285 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 28 VPVVYYSLLLCLGLPANILTVII 50 PRIMARY 23 2 64 NYLLALAAADILVLFFIVFVDFL 86 PRIMARY 23 3 110 SSIHTSIWITVPLTIDRYIAVCH 132 SECONDARY 23 4 148 VIVSVYITCFLTSIPYYWWP 167 SECONDARY 20 5 182 VLIWIHCFTVYLVPCSIFFILNS 204 PRIMARY 23 6 231 FTITSIFATLWAPRIIMILYHLY 253 SECONDARY 23 7 266 MSDIANMLALLNTAINFFLYCFI 288 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001318006 374 609046 45.7 82.2 NP_001318005 374 609046 45.7 82.2 NP_861455 462 609046 45.7 82.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9429 Download

>KIEE9429  1059 bp
ATGGAGCACACGCACGCCCACCTCGCAGCCAACAGCTCGCTGTCTTGGTGGTCCCCCGGC
TCGGCCTGCGGCTTGGGTTTCGTGCCCGTGGTCTACTACAGCCTCTTGCTGTGCCTCGGT
TTACCAGCAAATATCTTGACAGTGATCATCCTCTCCCAGCTGGTGGCAAGAAGACAGAAG
TCCTCCTACAACTATCTCTTGGCACTCGCTGCTGCCGACATCTTGGTCCTCTTTTTCATA
GTGTTTGTGGACTTCCTGTTGGAAGATTTCATCTTGAACATGCAGATGCCTCAGGTCCCC
GACAAGATCATAGAAGTGCTGGAATTCTCATCCATCCACACCTCCATATGGATTACTGTA
CCGTTAACCATTGACAGGTATATCGCTGTCTGCCACCCGCTCAAGTACCACACGGTCTCA
TACCCAGCCCGCACCCGGAAAGTCATTGTAAGTGTTTACATCACCTGCTTCCTGACCAGC
ATCCCCTATTACTGGTGGCCCAACATCTGGACTGAAGACTACATCAGCACCTCTGTGCAT
CACGTCCTCATCTGGATCCACTGCTTCACCGTCTACCTGGTGCCCTGCTCCATCTTCTTC
ATCTTGAACTCAATCATTGTGTACAAGCTCAGGAGGAAGAGCAATTTTCGTCTCCGTGGC
TACTCCACGGGGAAGACCACCGCCATCTTGTTCACCATTACCTCCATCTTTGCCACACTT
TGGGCCCCCCGCATCATCATGATTCTTTACCACCTCTATGGGGCGCCCATCCAGAACCGC
TGGCTGGTACACATCATGTCCGACATTGCCAACATGCTAGCCCTTCTGAACACAGCCATC
AACTTCTTCCTCTACTGCTTCATCAGCAAGCGGTTCCGCACCATGGCAGCCGCCACGCTC
AAGGCTTTCTTCAAGTGCCAGAAGCAACCTGTACAGTTCTACACCAATCATAACTTTTCC
ATAACAAGTAGCCCCTGGATCTCGCCGGCAAACTCACACTGCATCAAGATGCTGGTGTAC
CAGTATGACAAAAATGGAAAACCTATAAAAGTATCCCCG

ORF Protein

Cloned ORF protein sequence for pFN21AE9429 Download

>KIEE9429  353 aa
MEHTHAHLAANSSLSWWSPGSACGLGFVPVVYYSLLLCLGLPANILTVIILSQLVARRQK
SSYNYLLALAAADILVLFFIVFVDFLLEDFILNMQMPQVPDKIIEVLEFSSIHTSIWITV
PLTIDRYIAVCHPLKYHTVSYPARTRKVIVSVYITCFLTSIPYYWWPNIWTEDYISTSVH
HVLIWIHCFTVYLVPCSIFFILNSIIVYKLRRKSNFRLRGYSTGKTTAILFTITSIFATL
WAPRIIMILYHLYGAPIQNRWLVHIMSDIANMLALLNTAINFFLYCFISKRFRTMAAATL
KAFFKCQKQPVQFYTNHNFSITSSPWISPANSHCIKMLVYQYDKNGKPIKVSP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9429 Download

>pFN21AE9429  5764 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCACACGCACGCCCACCTCGCAGCCAACAG
CTCGCTGTCTTGGTGGTCCCCCGGCTCGGCCTGCGGCTTGGGTTTCGTGCCCGTGGTCTA
CTACAGCCTCTTGCTGTGCCTCGGTTTACCAGCAAATATCTTGACAGTGATCATCCTCTC
CCAGCTGGTGGCAAGAAGACAGAAGTCCTCCTACAACTATCTCTTGGCACTCGCTGCTGC
CGACATCTTGGTCCTCTTTTTCATAGTGTTTGTGGACTTCCTGTTGGAAGATTTCATCTT
GAACATGCAGATGCCTCAGGTCCCCGACAAGATCATAGAAGTGCTGGAATTCTCATCCAT
CCACACCTCCATATGGATTACTGTACCGTTAACCATTGACAGGTATATCGCTGTCTGCCA
CCCGCTCAAGTACCACACGGTCTCATACCCAGCCCGCACCCGGAAAGTCATTGTAAGTGT
TTACATCACCTGCTTCCTGACCAGCATCCCCTATTACTGGTGGCCCAACATCTGGACTGA
AGACTACATCAGCACCTCTGTGCATCACGTCCTCATCTGGATCCACTGCTTCACCGTCTA
CCTGGTGCCCTGCTCCATCTTCTTCATCTTGAACTCAATCATTGTGTACAAGCTCAGGAG
GAAGAGCAATTTTCGTCTCCGTGGCTACTCCACGGGGAAGACCACCGCCATCTTGTTCAC
CATTACCTCCATCTTTGCCACACTTTGGGCCCCCCGCATCATCATGATTCTTTACCACCT
CTATGGGGCGCCCATCCAGAACCGCTGGCTGGTACACATCATGTCCGACATTGCCAACAT
GCTAGCCCTTCTGAACACAGCCATCAACTTCTTCCTCTACTGCTTCATCAGCAAGCGGTT
CCGCACCATGGCAGCCGCCACGCTCAAGGCTTTCTTCAAGTGCCAGAAGCAACCTGTACA
GTTCTACACCAATCATAACTTTTCCATAACAAGTAGCCCCTGGATCTCGCCGGCAAACTC
ACACTGCATCAAGATGCTGGTGTACCAGTATGACAAAAATGGAAAACCTATAAAAGTATC
CCCGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT