GPR32 ORF clone pFN21AE9411

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Halotag ORF Clone pFN21AE9411

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Clone info.

Species

human

Clone Name

pFN21AE9411 Sequence (with vector) Restriction Map

Product ID

FHC26758

ORF Sequence

DNA sequence (1068 bp)
Protein sequence (356 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9411

Symbol

GPR32

Alias : RVDR1

Description

G protein-coupled receptor 32

Original Clone ID

cp99411

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1068 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 356 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12801.1 1.7e-120 100.0 GPR32 CCDS12840.1 3.2e-33 40.5 FPR2 CCDS12841.1 2.8e-32 37.5 FPR3 CCDS12839.1 3.8e-29 35.0 FPR1 CCDS41829.1 1.6e-27 36.0 CMKLR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 45 69 PR00237 GPCR IPR000826 69 82 PR00526 Formyl/methionyl peptide receptor IPR000276 77 98 PR00237 GPCR IPR000826 89 105 PR00526 Formyl/methionyl peptide receptor IPR000826 115 132 PR00526 Formyl/methionyl peptide receptor IPR000276 121 143 PR00237 GPCR IPR000826 140 156 PR00526 Formyl/methionyl peptide receptor IPR000276 217 240 PR00237 GPCR IPR000826 239 256 PR00526 Formyl/methionyl peptide receptor IPR000276 254 278 PR00237 GPCR IPR000276 298 324 PR00237 GPCR IPR000826 320 339 PR00526 Formyl/methionyl peptide receptor HMMPfam IPR000276 60 316 PF00001 GPCR ProfileScan IPR017452 60 316 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 43 RPLTVVILSASIVVGVLGNGLVL 65 PRIMARY 23 2 77 STVCFFHLALADFMLSLSLPIAM 99 PRIMARY 23 3 130 CLLVFISVDRCISVLYPVWALNH 152 PRIMARY 23 4 155 VQRASWLAFGVWLLAAALCSAH 176 PRIMARY 22 5 209 VVEGHIIGTIGHFLLGFLGPLAI 231 PRIMARY 23 6 257 LLLVLVSAFFIFWSPFNVVLLVH 279 PRIMARY 23 7 295 LLILQASFALGCVNSSLNPFLYV 317 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001497 356 603195 100.0 100.0 NP_001005738 351 136538 40.5 84.6 XP_006723183 351 136538 40.5 84.6 XP_016882031 351 136538 40.5 84.6 NP_001453 351 136538 40.5 84.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9411 Download

>KIEE9411  1068 bp
ATGAATGGGGTCTCGGAGGGGACCAGAGGCTGCAGTGACAGGCAACCTGGGGTCCTGACA
CGTGATCGCTCTTGTTCCAGGAAGATGAACTCTTCCGGATGCCTGTCTGAGGAGGTGGGG
TCCCTCCGCCCACTGACTGTGGTTATCCTGTCTGCGTCCATTGTCGTCGGAGTGCTGGGC
AATGGGCTGGTGCTGTGGATGACTGTCTTCCGTATGGCACGCACGGTCTCCACCGTCTGC
TTCTTCCACCTGGCCCTTGCCGATTTCATGCTCTCACTGTCTCTGCCCATTGCCATGTAC
TATATTGTCTCCAGGCAGTGGCTCCTCGGAGAGTGGGCCTGCAAACTCTACATCACCTTT
GTGTTCCTCAGCTACTTTGCCAGTAACTGCCTCCTTGTCTTCATCTCTGTGGACCGTTGC
ATCTCTGTCCTCTACCCCGTCTGGGCCCTGAACCACCGCACTGTGCAGCGGGCGAGCTGG
CTGGCCTTTGGGGTGTGGCTCCTGGCCGCCGCCTTGTGCTCTGCGCACCTGAAATTCCGG
ACAACCAGAAAATGGAATGGCTGTACGCACTGCTACTTGGCGTTCAACTCTGACAATGAG
ACTGCCCAGATTTGGATTGAAGGGGTCGTGGAGGGACACATTATAGGGACCATTGGCCAC
TTCCTGCTGGGCTTCCTGGGGCCCTTAGCAATCATAGGCACCTGCGCCCACCTCATCCGG
GCCAAGCTCTTGCGGGAGGGCTGGGTCCATGCCAACCGGCCCAAGAGGCTGCTGCTGGTG
CTGGTGAGCGCTTTCTTTATCTTCTGGTCCCCGTTTAACGTGGTGCTGTTGGTCCATCTG
TGGCGACGGGTGATGCTCAAGGAAATCTACCACCCCCGGATGCTGCTCATCCTCCAGGCT
AGCTTTGCCTTGGGCTGTGTCAACAGCAGCCTCAACCCCTTCCTCTACGTCTTCGTTGGC
AGAGATTTCCAAGAAAAGTTTTTCCAGTCTTTGACTTCTGCCCTGGCGAGGGCGTTTGGA
GAGGAGGAGTTTCTGTCATCCTGTCCCCGTGGCAACGCCCCCCGGGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE9411 Download

>KIEE9411  356 aa
MNGVSEGTRGCSDRQPGVLTRDRSCSRKMNSSGCLSEEVGSLRPLTVVILSASIVVGVLG
NGLVLWMTVFRMARTVSTVCFFHLALADFMLSLSLPIAMYYIVSRQWLLGEWACKLYITF
VFLSYFASNCLLVFISVDRCISVLYPVWALNHRTVQRASWLAFGVWLLAAALCSAHLKFR
TTRKWNGCTHCYLAFNSDNETAQIWIEGVVEGHIIGTIGHFLLGFLGPLAIIGTCAHLIR
AKLLREGWVHANRPKRLLLVLVSAFFIFWSPFNVVLLVHLWRRVMLKEIYHPRMLLILQA
SFALGCVNSSLNPFLYVFVGRDFQEKFFQSLTSALARAFGEEEFLSSCPRGNAPRE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9411 Download

>pFN21AE9411  5773 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATGGGGTCTCGGAGGGGACCAGAGGCTGCAG
TGACAGGCAACCTGGGGTCCTGACACGTGATCGCTCTTGTTCCAGGAAGATGAACTCTTC
CGGATGCCTGTCTGAGGAGGTGGGGTCCCTCCGCCCACTGACTGTGGTTATCCTGTCTGC
GTCCATTGTCGTCGGAGTGCTGGGCAATGGGCTGGTGCTGTGGATGACTGTCTTCCGTAT
GGCACGCACGGTCTCCACCGTCTGCTTCTTCCACCTGGCCCTTGCCGATTTCATGCTCTC
ACTGTCTCTGCCCATTGCCATGTACTATATTGTCTCCAGGCAGTGGCTCCTCGGAGAGTG
GGCCTGCAAACTCTACATCACCTTTGTGTTCCTCAGCTACTTTGCCAGTAACTGCCTCCT
TGTCTTCATCTCTGTGGACCGTTGCATCTCTGTCCTCTACCCCGTCTGGGCCCTGAACCA
CCGCACTGTGCAGCGGGCGAGCTGGCTGGCCTTTGGGGTGTGGCTCCTGGCCGCCGCCTT
GTGCTCTGCGCACCTGAAATTCCGGACAACCAGAAAATGGAATGGCTGTACGCACTGCTA
CTTGGCGTTCAACTCTGACAATGAGACTGCCCAGATTTGGATTGAAGGGGTCGTGGAGGG
ACACATTATAGGGACCATTGGCCACTTCCTGCTGGGCTTCCTGGGGCCCTTAGCAATCAT
AGGCACCTGCGCCCACCTCATCCGGGCCAAGCTCTTGCGGGAGGGCTGGGTCCATGCCAA
CCGGCCCAAGAGGCTGCTGCTGGTGCTGGTGAGCGCTTTCTTTATCTTCTGGTCCCCGTT
TAACGTGGTGCTGTTGGTCCATCTGTGGCGACGGGTGATGCTCAAGGAAATCTACCACCC
CCGGATGCTGCTCATCCTCCAGGCTAGCTTTGCCTTGGGCTGTGTCAACAGCAGCCTCAA
CCCCTTCCTCTACGTCTTCGTTGGCAGAGATTTCCAAGAAAAGTTTTTCCAGTCTTTGAC
TTCTGCCCTGGCGAGGGCGTTTGGAGAGGAGGAGTTTCTGTCATCCTGTCCCCGTGGCAA
CGCCCCCCGGGAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT