GPR31 ORF clone pFN21AE9410

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Halotag ORF Clone pFN21AE9410

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Clone info.

Species

human

Clone Name

pFN21AE9410 Sequence (with vector) Restriction Map

Product ID

FHC22834

ORF Sequence

DNA sequence (957 bp)
Protein sequence (319 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9410

Symbol

GPR31

Alias : 12-HETER, HETER, HETER1

Description

G protein-coupled receptor 31

Original Clone ID

cp99410

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 957 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 319 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5299.1 5.3e-104 99.7 GPR31 CCDS53842.1 2.5e-29 36.7 HCAR3 CCDS9235.1 7e-29 36.1 HCAR2 CCDS9236.1 7.2e-26 34.4 HCAR1 CCDS1810.1 3.1e-24 33.7 OXER1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 16 40 PR00237 GPCR IPR000276 49 70 PR00237 GPCR IPR000276 94 116 PR00237 GPCR IPR000276 130 151 PR00237 GPCR IPR000276 178 201 PR00237 GPCR IPR000276 220 244 PR00237 GPCR IPR000276 264 290 PR00237 GPCR HMMPfam IPR000276 31 282 PF00001 GPCR ProfileScan IPR017452 31 282 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 14 ATAVGVLLGLECGLGLLGNAVAL 36 PRIMARY 23 2 52 YLLNLALADLLLAACLPFLAAFY 74 PRIMARY 23 3 89 ALRFLLDLSRSVGMAFLAAVALD 111 SECONDARY 23 4 133 GVSGLVWLLMVALTCPGLLISEA 155 PRIMARY 23 5 183 CLQFVLPFGLIVFCNAGIIRALQ 205 PRIMARY 23 6 223 LVTLVVVLFALCFLPCFLARVLM 245 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005290 319 602043 99.7 100.0 NP_006009 387 606039 36.7 95.9 NP_808219 363 609163 36.1 95.9 NP_115943 346 606923 34.4 91.2 NP_788086 377 600041 32.5 82.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9410 Download

>KIEE9410  957 bp
ATGCCATTCCCAAACTGCTCAGCCCCCAGCACTGTGGTGGCCACAGCTGTGGGTGTCTTG
CTGGGGCTGGAGTGTGGGCTGGGTCTGCTGGGCAACGCGGTGGCGCTGTGGACCTTCCTG
TTCCGGGTCAGGGTGTGGAAGCCGTACGCTGTCTACCTGCTCAACCTGGCCCTGGCTGAC
CTGCTGTTGGCTGCGTGCCTGCCTTTCCTGGCCGCCTTCTACCTGAGCCTCCAGGCTTGG
CATCTGGGCCGTGTGGGCTGCTGGGCCCTGCGCTTCCTGCTGGACCTCAGCCGCAGCGTG
GGGATGGCCTTCCTGGCCGCCGTGGCTTTGGACCGGTACCTCCGTGTGGTCCACCCTCGG
CTTAAGGTCAACCTGCTGTCTCCTCAGGCGGCCCTGGGGGTCTCGGGCCTCGTCTGGCTC
CTGATGGTCGCCCTCACCTGCCCGGGCTTGCTCATCTCTGAGGCCGCCCAGAACTCCACC
AGGTGCCACAGTTTCTACTCCAGGGCAGACGGCTCCTTCAGCATCATCTGGCAGGAAGCA
CTCTCCTGCCTTCAGTTTGTCCTCCCCTTTGGCCTCATCGTGTTCTGCAATGCAGGCATC
ATCAGGGCTCTCCAGAAAAGACTCCGGGAGCCTGAGAAACAGCCCAAGCTTCAGCGGGCC
CAGGCACTGGTCACCTTGGTGGTGGTGCTGTTTGCTCTGTGCTTTCTGCCCTGCTTCCTG
GCCAGAGTCCTGATGCACATCTTCCAGAATCTGGGGAGCTGCAGGGCCCTTTGTGCAGTG
GCTCATACCTCGGATGTCACGGGCAGCCTCACCTACCTGCACAGTGTGCTCAACCCCGTG
GTATACTGCTTCTCCAGCCCCACCTTCAGGAGCTCCTATCGGAGGGTCTTCCACACCCTC
CGAGGCAAAGGGCAGGCAGCAGAGCCCCCAGATTTCAACCCCAGAGACTCCTATTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9410 Download

>KIEE9410  319 aa
MPFPNCSAPSTVVATAVGVLLGLECGLGLLGNAVALWTFLFRVRVWKPYAVYLLNLALAD
LLLAACLPFLAAFYLSLQAWHLGRVGCWALRFLLDLSRSVGMAFLAAVALDRYLRVVHPR
LKVNLLSPQAALGVSGLVWLLMVALTCPGLLISEAAQNSTRCHSFYSRADGSFSIIWQEA
LSCLQFVLPFGLIVFCNAGIIRALQKRLREPEKQPKLQRAQALVTLVVVLFALCFLPCFL
ARVLMHIFQNLGSCRALCAVAHTSDVTGSLTYLHSVLNPVVYCFSSPTFRSSYRRVFHTL
RGKGQAAEPPDFNPRDSYS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9410 Download

>pFN21AE9410  5662 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCATTCCCAAACTGCTCAGCCCCCAGCACTGT
GGTGGCCACAGCTGTGGGTGTCTTGCTGGGGCTGGAGTGTGGGCTGGGTCTGCTGGGCAA
CGCGGTGGCGCTGTGGACCTTCCTGTTCCGGGTCAGGGTGTGGAAGCCGTACGCTGTCTA
CCTGCTCAACCTGGCCCTGGCTGACCTGCTGTTGGCTGCGTGCCTGCCTTTCCTGGCCGC
CTTCTACCTGAGCCTCCAGGCTTGGCATCTGGGCCGTGTGGGCTGCTGGGCCCTGCGCTT
CCTGCTGGACCTCAGCCGCAGCGTGGGGATGGCCTTCCTGGCCGCCGTGGCTTTGGACCG
GTACCTCCGTGTGGTCCACCCTCGGCTTAAGGTCAACCTGCTGTCTCCTCAGGCGGCCCT
GGGGGTCTCGGGCCTCGTCTGGCTCCTGATGGTCGCCCTCACCTGCCCGGGCTTGCTCAT
CTCTGAGGCCGCCCAGAACTCCACCAGGTGCCACAGTTTCTACTCCAGGGCAGACGGCTC
CTTCAGCATCATCTGGCAGGAAGCACTCTCCTGCCTTCAGTTTGTCCTCCCCTTTGGCCT
CATCGTGTTCTGCAATGCAGGCATCATCAGGGCTCTCCAGAAAAGACTCCGGGAGCCTGA
GAAACAGCCCAAGCTTCAGCGGGCCCAGGCACTGGTCACCTTGGTGGTGGTGCTGTTTGC
TCTGTGCTTTCTGCCCTGCTTCCTGGCCAGAGTCCTGATGCACATCTTCCAGAATCTGGG
GAGCTGCAGGGCCCTTTGTGCAGTGGCTCATACCTCGGATGTCACGGGCAGCCTCACCTA
CCTGCACAGTGTGCTCAACCCCGTGGTATACTGCTTCTCCAGCCCCACCTTCAGGAGCTC
CTATCGGAGGGTCTTCCACACCCTCCGAGGCAAAGGGCAGGCAGCAGAGCCCCCAGATTT
CAACCCCAGAGACTCCTATTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT