GPR174 ORF clone pFN21AE9407

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Halotag ORF Clone pFN21AE9407

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Clone info.

Species

human

Clone Name

pFN21AE9407 Sequence (with vector) Restriction Map

Product ID

FHC27433

Flexi Clone PID

FXC27433

ORF Sequence

DNA sequence (999 bp)
Protein sequence (333 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9407

Symbol

GPR174

Alias : FKSG79, GPCR17, LYPSR3

Description

G protein-coupled receptor 174

Original Clone ID

cp99407

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 999 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 333 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14443.1 4.2e-114 100.0 GPR174 CCDS14442.1 1.3e-50 49.7 P2RY10 CCDS14441.1 2.3e-29 34.2 LPAR4 CCDS9410.1 7.1e-28 31.0 LPAR6 CCDS14439.1 6.9e-26 33.4 CYSLTR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 21 45 PR00237 GPCR IPR000276 54 75 PR00237 GPCR IPR002286 68 79 PR01157 P2 purinoceptor IPR002286 91 108 PR01157 P2 purinoceptor IPR000276 98 120 PR00237 GPCR IPR002286 115 125 PR01157 P2 purinoceptor IPR000276 186 209 PR00237 GPCR IPR000276 228 252 PR00237 GPCR IPR002286 242 253 PR01157 P2 purinoceptor IPR000276 274 300 PR00237 GPCR HMMPfam IPR000276 36 292 PF00001 GPCR ProfileScan IPR017452 36 292 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 21 IYAVTYTVILVPGLIGNILALWV 43 PRIMARY 23 2 55 VIFMINLAIADLLQVLSLPLRIF 77 PRIMARY 23 3 100 VNMYASIYFLVCISVRRFWFLMY 122 SECONDARY 23 4 136 YISIAGWLIICLACVLFPLLRTS 158 PRIMARY 23 5 181 QSVVMMTIGELIGFVTPLLIVLY 203 PRIMARY 23 6 227 KALKMILTCAGVFLICFAPYH 247 PRIMARY 21 7 270 ILIFHSVALCLASLNSCLDPVIY 292 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115942 333 300903 100.0 100.0 NP_001311147 339 300529 49.7 94.6 NP_055314 339 300529 49.7 94.6 NP_938147 339 300529 49.7 94.6 NP_001311150 356 300529 49.7 94.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9407 Download

>KIEE9407  999 bp
ATGCCTGCTAATTACACGTGTACCAGGCCAGATGGAGACAATACAGATTTTCGATACTTT
ATTTATGCAGTGACATACACTGTCATTCTTGTGCCAGGTCTCATAGGGAATATATTAGCC
CTGTGGGTATTCTATGGTTATATGAAAGAAACAAAACGAGCTGTGATATTTATGATAAAC
TTAGCCATTGCTGACTTACTACAAGTTCTTTCCTTGCCACTGAGGATCTTCTACTACTTG
AATCATGACTGGCCATTTGGGCCTGGTCTCTGCATGTTCTGTTTCTACCTGAAGTATGTC
AACATGTATGCAAGCATCTACTTCTTGGTCTGCATCAGTGTGCGACGATTTTGGTTTCTC
ATGTACCCCTTTCGCTTCCATGACTGCAAACAGAAATATGACCTGTACATCAGCATTGCT
GGCTGGCTGATCATCTGCCTTGCCTGTGTACTCTTTCCACTCCTCAGAACCAGTGATGAT
ACCTCTGGCAATAGGACCAAATGCTTTGTGGATCTTCCTACCAGGAATGTCAACCTGGCC
CAGTCCGTTGTTATGATGACCATTGGCGAGTTGATTGGGTTTGTAACTCCGCTTCTGATT
GTCCTATATTGTACCTGGAAGACGGTTTTATCACTGCAAGATAAATATCCCATGGCCCAA
GATCTTGGAGAGAAACAGAAAGCCTTGAAGATGATTCTAACCTGTGCAGGGGTATTCCTA
ATTTGCTTTGCACCTTATCATTTCAGTTTTCCTTTAGATTTCCTGGTGAAGTCCAATGAA
ATTAAAAGCTGCCTAGCCAGAAGGGTGATTCTAATATTTCATTCTGTGGCATTGTGTCTT
GCTAGTCTGAATTCATGTCTTGACCCAGTCATATACTACTTTTCCACTAATGAGTTCCGA
AGACGGCTTTCAAGACAAGATTTGCATGACAGCATCCAACTCCATGCAAAATCCTTTGTG
AGTAACCATACAGCTTCCACCATGACACCTGAATTATGC

ORF Protein

Cloned ORF protein sequence for pFN21AE9407 Download

>KIEE9407  333 aa
MPANYTCTRPDGDNTDFRYFIYAVTYTVILVPGLIGNILALWVFYGYMKETKRAVIFMIN
LAIADLLQVLSLPLRIFYYLNHDWPFGPGLCMFCFYLKYVNMYASIYFLVCISVRRFWFL
MYPFRFHDCKQKYDLYISIAGWLIICLACVLFPLLRTSDDTSGNRTKCFVDLPTRNVNLA
QSVVMMTIGELIGFVTPLLIVLYCTWKTVLSLQDKYPMAQDLGEKQKALKMILTCAGVFL
ICFAPYHFSFPLDFLVKSNEIKSCLARRVILIFHSVALCLASLNSCLDPVIYYFSTNEFR
RRLSRQDLHDSIQLHAKSFVSNHTASTMTPELC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9407 Download

>pFN21AE9407  5704 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTGCTAATTACACGTGTACCAGGCCAGATGG
AGACAATACAGATTTTCGATACTTTATTTATGCAGTGACATACACTGTCATTCTTGTGCC
AGGTCTCATAGGGAATATATTAGCCCTGTGGGTATTCTATGGTTATATGAAAGAAACAAA
ACGAGCTGTGATATTTATGATAAACTTAGCCATTGCTGACTTACTACAAGTTCTTTCCTT
GCCACTGAGGATCTTCTACTACTTGAATCATGACTGGCCATTTGGGCCTGGTCTCTGCAT
GTTCTGTTTCTACCTGAAGTATGTCAACATGTATGCAAGCATCTACTTCTTGGTCTGCAT
CAGTGTGCGACGATTTTGGTTTCTCATGTACCCCTTTCGCTTCCATGACTGCAAACAGAA
ATATGACCTGTACATCAGCATTGCTGGCTGGCTGATCATCTGCCTTGCCTGTGTACTCTT
TCCACTCCTCAGAACCAGTGATGATACCTCTGGCAATAGGACCAAATGCTTTGTGGATCT
TCCTACCAGGAATGTCAACCTGGCCCAGTCCGTTGTTATGATGACCATTGGCGAGTTGAT
TGGGTTTGTAACTCCGCTTCTGATTGTCCTATATTGTACCTGGAAGACGGTTTTATCACT
GCAAGATAAATATCCCATGGCCCAAGATCTTGGAGAGAAACAGAAAGCCTTGAAGATGAT
TCTAACCTGTGCAGGGGTATTCCTAATTTGCTTTGCACCTTATCATTTCAGTTTTCCTTT
AGATTTCCTGGTGAAGTCCAATGAAATTAAAAGCTGCCTAGCCAGAAGGGTGATTCTAAT
ATTTCATTCTGTGGCATTGTGTCTTGCTAGTCTGAATTCATGTCTTGACCCAGTCATATA
CTACTTTTCCACTAATGAGTTCCGAAGACGGCTTTCAAGACAAGATTTGCATGACAGCAT
CCAACTCCATGCAAAATCCTTTGTGAGTAACCATACAGCTTCCACCATGACACCTGAATT
ATGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT