EME1 ORF clone pFN21AE6758

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6758

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Clone info.

Species

human

Clone Name

pFN21AE6758 Sequence (with vector) Restriction Map

Product ID

FHC15815

ORF Sequence

DNA sequence (1749 bp)
Protein sequence (583 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6758

Symbol

EME1

Alias : MMS4L, SLX2A

Description

essential meiotic structure-specific endonuclease 1, transcript variant 1

Original Clone ID

oc96758

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1749 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 583 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS54141.1 4.4e-209 99.8 EME1 CCDS11565.1 1.2e-131 97.6 EME1 CCDS58404.1 3.1e-22 35.3 EME2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006166 254 528 PF02732 ERCC4 domain HMMSmart IPR006166 253 528 SM00891 ERCC4 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001159603 583 610885 99.8 100.0 NP_689676 570 610885 97.6 100.0 XP_016879725 541 610885 92.6 100.0 XP_005257138 413 610885 70.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6758 Download

>KIEE6758  1749 bp
ATGGCTCTAAAGAAGTCATCACCCTCACTGGATTCTGGTGATAGTGACTCTGAGGAGTTG
CCAACATTTGCCTTTCTGAAGAAGGAACCATCTTCAACAAAGAGGAGACAGCCTGAAAGG
GAAGAGAAGATTGTAGTGGTTGACATCTCAGATTGTGAAGCCTCCTGTCCTCCAGCACCA
GAGTTATTTTCACCACCTGTCCCAGACATAGCTGAAACTGTCACACAAACACAGCCAGTC
AGGTTGCTAAGCAGTGAAAGTGAAGATGAAGAAGAATTTATTCCTCTGGCTCAAAGGCTT
ACATGTAAGTTTCTGACCCACAAGCAACTGAGCCCTGAGGACTCTAGCTCCCCAGTTAAA
AGTGTTTTGGATCATCAAAATAATGAAGGTGCATCATGTGACTGGAAAAAGCCCTTTCCA
AAGATCCCTGAAGTTCCCCTCCATGATACCCCAGAGAGGAGTGCAGCAGATAACAAGGAC
CTGATCTTAGATCCATGCTGTCAGCTTCCAGCCTACCTGTCTACCTGCCCTGGCCAGAGC
AGCAGCTTGGCAGTAACCAAAACAAATTCTGACATCCTTCCACCCCAGAAGAAAACCAAG
CCGAGTCAGAAGGTCCAGGGAAGAGGCTCACACGGATGCCGGCAGCAGAGACAAGCAAGG
CAGAAGGAAAGCACCCTGAGAAGACAGGAAAGAAAGAATGCAGCACTGGTTACCAGGATG
AAAGCCCAGAGGCCAGAGGAATGCTTAAAACACATCATTGTAGTGCTGGATCCAGTGCTC
TTACAGATGGAAGGTGGGGGCCAGCTCCTAGGAGCACTGCAGACCATGGAGTGCCGCTGT
GTGATTGAGGCGCAGGCTGTGCCTTGCAGTGTCACTTGGAGGAGAAGGGCTGGGCCGTCT
GAGGACAGAGAGGACTGGGTGGAGGAGCCAACAGTACTGGTGTTGCTCCGGGCAGAGGCA
TTTGTGTCCATGATCGACAATGGAAAGCAGGGAAGCCTGGACAGCACTATGAAAGGGAAG
GAAACGCTTCAGGGCTTTGTAACTGACATCACAGCAAAGACAGCAGGGAAAGCTCTGTCA
CTGGTGATTGTGGATCAGGAGAAATGCTTCAGCCTGGAGCTGCTGTTCTTTGATTTCCTC
CCCTGCACCAGTGCTCAGAATCCTCCAAGAAGAGGGAAACAGGGAGCAAATAAACAGACC
AAGAAGCAGCAGCAGAGACAACCAGAGGCCAGCATAGGGTCCATGGTATCCAGGGTAGAC
GCTGAAGAGGCATTGGTGGATCTGCAGCTACACACAGAAGCCCAGGCTCAAATTGTGCAG
AGCTGGAAAGAGCTGGCCGACTTCACATGCGCATTCACAAAGGCTGTGGCTGAGGCGCCC
TTCAAGAAGCTCCGAGATGAAACTACCTTCTCCTTCTGTCTGGAGAGTGACTGGGCTGGA
GGGGTGAAGGTGGACCTTGCTGGCAGGGGACTCGCACTAGTCTGGAGGAGACAGATTCAG
CAGCTGAACCGAGTCAGCCTGGAAATGGCCAGTGCAGTTGTGAATGCCTATCCCTCCCCA
CAGCTCCTGGTACAGGCTTATCAGCAGTGTTTTTCGGATAAAGAACGCCAGAATTTGCTC
GCAGACATACAGGTGCGCCGTGGGGAAGGTGTGACATCCACTTCTCGCCGCATTGGACCA
GAACTATCCAGGCGTATCTACCTTCAGATGACCACTTTACAGCCACATCTCTCTTTAGAT
AGTGCTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6758 Download

>KIEE6758  583 aa
MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAP
ELFSPPVPDIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVK
SVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQS
SSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRM
KAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPS
EDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALS
LVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVD
AEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAG
GVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLL
ADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6758 Download

>pFN21AE6758  6454 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTCTAAAGAAGTCATCACCCTCACTGGATTC
TGGTGATAGTGACTCTGAGGAGTTGCCAACATTTGCCTTTCTGAAGAAGGAACCATCTTC
AACAAAGAGGAGACAGCCTGAAAGGGAAGAGAAGATTGTAGTGGTTGACATCTCAGATTG
TGAAGCCTCCTGTCCTCCAGCACCAGAGTTATTTTCACCACCTGTCCCAGACATAGCTGA
AACTGTCACACAAACACAGCCAGTCAGGTTGCTAAGCAGTGAAAGTGAAGATGAAGAAGA
ATTTATTCCTCTGGCTCAAAGGCTTACATGTAAGTTTCTGACCCACAAGCAACTGAGCCC
TGAGGACTCTAGCTCCCCAGTTAAAAGTGTTTTGGATCATCAAAATAATGAAGGTGCATC
ATGTGACTGGAAAAAGCCCTTTCCAAAGATCCCTGAAGTTCCCCTCCATGATACCCCAGA
GAGGAGTGCAGCAGATAACAAGGACCTGATCTTAGATCCATGCTGTCAGCTTCCAGCCTA
CCTGTCTACCTGCCCTGGCCAGAGCAGCAGCTTGGCAGTAACCAAAACAAATTCTGACAT
CCTTCCACCCCAGAAGAAAACCAAGCCGAGTCAGAAGGTCCAGGGAAGAGGCTCACACGG
ATGCCGGCAGCAGAGACAAGCAAGGCAGAAGGAAAGCACCCTGAGAAGACAGGAAAGAAA
GAATGCAGCACTGGTTACCAGGATGAAAGCCCAGAGGCCAGAGGAATGCTTAAAACACAT
CATTGTAGTGCTGGATCCAGTGCTCTTACAGATGGAAGGTGGGGGCCAGCTCCTAGGAGC
ACTGCAGACCATGGAGTGCCGCTGTGTGATTGAGGCGCAGGCTGTGCCTTGCAGTGTCAC
TTGGAGGAGAAGGGCTGGGCCGTCTGAGGACAGAGAGGACTGGGTGGAGGAGCCAACAGT
ACTGGTGTTGCTCCGGGCAGAGGCATTTGTGTCCATGATCGACAATGGAAAGCAGGGAAG
CCTGGACAGCACTATGAAAGGGAAGGAAACGCTTCAGGGCTTTGTAACTGACATCACAGC
AAAGACAGCAGGGAAAGCTCTGTCACTGGTGATTGTGGATCAGGAGAAATGCTTCAGCCT
GGAGCTGCTGTTCTTTGATTTCCTCCCCTGCACCAGTGCTCAGAATCCTCCAAGAAGAGG
GAAACAGGGAGCAAATAAACAGACCAAGAAGCAGCAGCAGAGACAACCAGAGGCCAGCAT
AGGGTCCATGGTATCCAGGGTAGACGCTGAAGAGGCATTGGTGGATCTGCAGCTACACAC
AGAAGCCCAGGCTCAAATTGTGCAGAGCTGGAAAGAGCTGGCCGACTTCACATGCGCATT
CACAAAGGCTGTGGCTGAGGCGCCCTTCAAGAAGCTCCGAGATGAAACTACCTTCTCCTT
CTGTCTGGAGAGTGACTGGGCTGGAGGGGTGAAGGTGGACCTTGCTGGCAGGGGACTCGC
ACTAGTCTGGAGGAGACAGATTCAGCAGCTGAACCGAGTCAGCCTGGAAATGGCCAGTGC
AGTTGTGAATGCCTATCCCTCCCCACAGCTCCTGGTACAGGCTTATCAGCAGTGTTTTTC
GGATAAAGAACGCCAGAATTTGCTCGCAGACATACAGGTGCGCCGTGGGGAAGGTGTGAC
ATCCACTTCTCGCCGCATTGGACCAGAACTATCCAGGCGTATCTACCTTCAGATGACCAC
TTTACAGCCACATCTCTCTTTAGATAGTGCTGACGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT