MGAT2 ORF clone pFN21AE6433

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Halotag ORF Clone pFN21AE6433

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Clone info.

Species

human

Clone Name

pFN21AE6433 Sequence (with vector) Restriction Map

Product ID

FHC08286

ORF Sequence

DNA sequence (1341 bp)
Protein sequence (447 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6433

Symbol

MGAT2

Alias : CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2

Description

mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

Original Clone ID

oc96433

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1341 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 447 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9690.1 5.6e-209 100.0 MGAT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007754 86 440 PF05060 N-acetylglucosaminyltransferase II ProfileScan NULL 1 20 PS51257 NULL
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 10 VLILTLVVAACGFVLW 25 PRIMARY 16
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002399 447 212066,602616 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6433 Download

>KIEE6433  1341 bp
ATGAGGTTCCGCATCTACAAACGGAAGGTGCTAATCCTGACGCTCGTGGTGGCCGCCTGC
GGCTTCGTCCTCTGGAGCAGCAATGGGCGACAAAGGAAGAACGAGGCCCTCGCCCCACCG
TTGCTGGACGCCGAACCCGCGCGGGGTGCCGGCGGCCGCGGTGGGGACCACCCCTCTGTG
GCTGTGGGCATCCGCAGGGTCTCCAACGTGTCGGCGGCTTCCCTGGTCCCGGCGGTCCCC
CAGCCCGAGGCGGACAACCTGACGCTGCGGTACCGGTCCCTGGTGTACCAGCTGAACTTT
GATCAGACCCTGAGGAATGTAGATAAGGCTGGCACCTGGGCCCCCCGGGAGCTGGTGCTG
GTGGTCCAGGTGCATAACCGGCCCGAATACCTCAGACTGCTGCTGGACTCACTTCGAAAA
GCCCAGGGAATTGACAACGTCCTCGTCATCTTTAGCCATGACTTCTGGTCGACCGAGATC
AATCAGCTGATCGCCGGGGTGAATTTCTGTCCGGTTCTGCAGGTGTTCTTTCCTTTCAGC
ATTCAGTTGTACCCTAACGAGTTTCCAGGTAGTGACCCTAGAGATTGTCCCAGAGACCTG
CCGAAGAATGCCGCTTTGAAATTGGGGTGCATCAATGCTGAGTATCCCGACTCCTTCGGC
CATTATAGAGAGGCCAAATTCTCCCAGACCAAACATCACTGGTGGTGGAAGCTGCATTTT
GTGTGGGAAAGAGTGAAAATTCTTCGAGATTATGCTGGCCTTATACTTTTCCTAGAAGAG
GATCACTACTTAGCCCCAGACTTTTACCATGTCTTCAAAAAGATGTGGAAACTGAAGCAG
CAAGAGTGCCCTGAATGTGATGTTCTCTCCCTGGGGACCTATAGTGCCAGTCGCAGTTTC
TATGGCATGGCTGACAAGGTAGATGTGAAAACTTGGAAATCCACAGAGCACAATATGGGT
CTAGCCTTGACCCGGAATGCCTATCAGAAGCTGATCGAGTGCACAGACACTTTCTGTACT
TATGATGATTATAACTGGGACTGGACTCTTCAATACTTGACTGTATCTTGTCTTCCAAAA
TTCTGGAAAGTGCTGGTTCCTCAAATTCCTAGGATCTTTCATGCTGGAGACTGTGGTATG
CATCACAAGAAAACCTGTAGACCATCCACTCAGAGTGCCCAAATTGAGTCACTCTTAAAT
AATAACAAACAATACATGTTTCCAGAAACTCTAACTATCAGTGAAAAGTTTACTGTGGTA
GCCATTTCCCCACCTAGAAAAAATGGAGGGTGGGGAGATATTAGGGACCATGAACTCTGT
AAAAGTTATAGAAGACTGCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE6433 Download

>KIEE6433  447 aa
MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKNEALAPPLLDAEPARGAGGRGGDHPSV
AVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVL
VVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFS
IQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHF
VWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSF
YGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPK
FWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVV
AISPPRKNGGWGDIRDHELCKSYRRLQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6433 Download

>pFN21AE6433  6046 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGTTCCGCATCTACAAACGGAAGGTGCTAAT
CCTGACGCTCGTGGTGGCCGCCTGCGGCTTCGTCCTCTGGAGCAGCAATGGGCGACAAAG
GAAGAACGAGGCCCTCGCCCCACCGTTGCTGGACGCCGAACCCGCGCGGGGTGCCGGCGG
CCGCGGTGGGGACCACCCCTCTGTGGCTGTGGGCATCCGCAGGGTCTCCAACGTGTCGGC
GGCTTCCCTGGTCCCGGCGGTCCCCCAGCCCGAGGCGGACAACCTGACGCTGCGGTACCG
GTCCCTGGTGTACCAGCTGAACTTTGATCAGACCCTGAGGAATGTAGATAAGGCTGGCAC
CTGGGCCCCCCGGGAGCTGGTGCTGGTGGTCCAGGTGCATAACCGGCCCGAATACCTCAG
ACTGCTGCTGGACTCACTTCGAAAAGCCCAGGGAATTGACAACGTCCTCGTCATCTTTAG
CCATGACTTCTGGTCGACCGAGATCAATCAGCTGATCGCCGGGGTGAATTTCTGTCCGGT
TCTGCAGGTGTTCTTTCCTTTCAGCATTCAGTTGTACCCTAACGAGTTTCCAGGTAGTGA
CCCTAGAGATTGTCCCAGAGACCTGCCGAAGAATGCCGCTTTGAAATTGGGGTGCATCAA
TGCTGAGTATCCCGACTCCTTCGGCCATTATAGAGAGGCCAAATTCTCCCAGACCAAACA
TCACTGGTGGTGGAAGCTGCATTTTGTGTGGGAAAGAGTGAAAATTCTTCGAGATTATGC
TGGCCTTATACTTTTCCTAGAAGAGGATCACTACTTAGCCCCAGACTTTTACCATGTCTT
CAAAAAGATGTGGAAACTGAAGCAGCAAGAGTGCCCTGAATGTGATGTTCTCTCCCTGGG
GACCTATAGTGCCAGTCGCAGTTTCTATGGCATGGCTGACAAGGTAGATGTGAAAACTTG
GAAATCCACAGAGCACAATATGGGTCTAGCCTTGACCCGGAATGCCTATCAGAAGCTGAT
CGAGTGCACAGACACTTTCTGTACTTATGATGATTATAACTGGGACTGGACTCTTCAATA
CTTGACTGTATCTTGTCTTCCAAAATTCTGGAAAGTGCTGGTTCCTCAAATTCCTAGGAT
CTTTCATGCTGGAGACTGTGGTATGCATCACAAGAAAACCTGTAGACCATCCACTCAGAG
TGCCCAAATTGAGTCACTCTTAAATAATAACAAACAATACATGTTTCCAGAAACTCTAAC
TATCAGTGAAAAGTTTACTGTGGTAGCCATTTCCCCACCTAGAAAAAATGGAGGGTGGGG
AGATATTAGGGACCATGAACTCTGTAAAAGTTATAGAAGACTGCAGGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT