CHST8 ORF clone pFN21AE6341

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Halotag ORF Clone pFN21AE6341

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Clone info.

Species

human

Clone Name

pFN21AE6341 Sequence (with vector) Restriction Map

Product ID

FHC08550

ORF Sequence

DNA sequence (1272 bp)
Protein sequence (424 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6341

Symbol

CHST8

Alias : GALNAC4ST1, GalNAc4ST, PSS3

Description

carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8, transcript variant 3

Original Clone ID

oc96341

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1272 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 424 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12433.1 2.1e-140 100.0 CHST8 CCDS42422.1 5.6e-60 48.1 CHST9 CCDS55878.1 1.9e-31 41.8 CHST11 CCDS9099.1 1.9e-31 41.8 CHST11 CCDS3039.1 6.5e-26 38.4 CHST13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005331 183 417 PF03567 Sulfotransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 10 LACMFSSILLFGAAGLLLFISLQ 32 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016882632 424 610190,616265 100.0 100.0 NP_071912 424 610190,616265 100.0 100.0 XP_011525526 424 610190,616265 100.0 100.0 NP_001121368 424 610190,616265 100.0 100.0 XP_011525524 424 610190,616265 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6341 Download

>KIEE6341  1272 bp
ATGACCCTGCGACCTGGAACAATGCGGCTGGCCTGCATGTTCTCTTCCATCCTGCTGTTC
GGAGCTGCAGGCCTCCTCCTCTTCATCAGCCTGCAGGACCCTACGGAGCTCGCCCCCCAG
CAGGTGCCAGGAATAAAGTTCAACATCAGGCCAAGGCAGCCCCACCACGACCTCCCACCA
GGCGGCTCCCAGGATGGTGACTTGAAGGAACCCACAGAGAGGGTCACTCGGGACTTATCC
AGTGGGGCCCCGAGGGGCCGCAACCTGCCAGCGCCTGACCAGCCTCAACCCCCGCTGCAG
AGGGGAACCCGTCTGCGGCTCCGCCAGCGCCGTCGCCGTCTGCTCATCAAGAAAATGCCA
GCTGCGGCGACCATCCCGGCCAACAGCTCGGACGCGCCCTTCATCCGGCCGGGACCCGGG
ACGCTGGATGGCCGCTGGGTCAGCCTGCACCGGAGCCAGCAGGAGCGCAAGCGGGTGATG
CAGGAGGCCTGCGCCAAGTACCGGGCGAGCAGCAGCCGCCGGGCCGTCACGCCCCGCCAC
GTGTCCCGTATCTTCGTGGAGGACCGCCACCGCGTGCTCTACTGCGAGGTGCCCAAGGCC
GGCTGCTCCAATTGGAAGCGGGTGCTCATGGTGCTGGCCGGCCTGGCCTCGTCCACTGCC
GACATCCAGCACAACACCGTCCACTATGGCAGCGCTCTCAAGCGCCTGGACACCTTCGAC
CGCCAGGGTATCTTGCACCGTCTCAGCACCTACACCAAGATGCTCTTTGTCCGCGAGCCC
TTCGAGAGGCTGGTGTCCGCCTTCCGCGACAAGTTTGAGCACCCCAACAGCTACTATCAC
CCGGTCTTCGGCAAGGCCATCCTGGCCCGGTACCGCGCCAATGCCTCTCGGGAGGCCCTG
CGGACCGGCTCTGGGGTGCGTTTTCCCGAGTTCGTCCAGTACCTGCTGGACGTGCACCGG
CCCGTGGGGATGGACATTCACTGGGACCATGTCAGCCGGCTCTGCAGCCCCTGCCTCATC
GACTACGATTTCGTAGGCAAGTTCGAGAGCATGGAGGACGATGCCAACTTCTTCCTGAGC
CTCATCCGCGCGCCGCGGAACCTGACCTTCCCCCGGTTCAAGGACCGGCACTCGCAGGAG
GCGCGGACCACAGCGAGGATCGCCCACCAGTACTTCGCCCAACTCTCGGCCCTGCAAAGG
CAGCGCACCTACGACTTCTACTACATGGATTACCTGATGTTCAACTATTCCAAGCCCTTT
GCAGATCTGTAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6341 Download

>KIEE6341  424 aa
MTLRPGTMRLACMFSSILLFGAAGLLLFISLQDPTELAPQQVPGIKFNIRPRQPHHDLPP
GGSQDGDLKEPTERVTRDLSSGAPRGRNLPAPDQPQPPLQRGTRLRLRQRRRRLLIKKMP
AAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQERKRVMQEACAKYRASSSRRAVTPRH
VSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASSTADIQHNTVHYGSALKRLDTFD
RQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPNSYYHPVFGKAILARYRANASREAL
RTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRLCSPCLIDYDFVGKFESMEDDANFFLS
LIRAPRNLTFPRFKDRHSQEARTTARIAHQYFAQLSALQRQRTYDFYYMDYLMFNYSKPF
ADLY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6341 Download

>pFN21AE6341  5977 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCCTGCGACCTGGAACAATGCGGCTGGCCTG
CATGTTCTCTTCCATCCTGCTGTTCGGAGCTGCAGGCCTCCTCCTCTTCATCAGCCTGCA
GGACCCTACGGAGCTCGCCCCCCAGCAGGTGCCAGGAATAAAGTTCAACATCAGGCCAAG
GCAGCCCCACCACGACCTCCCACCAGGCGGCTCCCAGGATGGTGACTTGAAGGAACCCAC
AGAGAGGGTCACTCGGGACTTATCCAGTGGGGCCCCGAGGGGCCGCAACCTGCCAGCGCC
TGACCAGCCTCAACCCCCGCTGCAGAGGGGAACCCGTCTGCGGCTCCGCCAGCGCCGTCG
CCGTCTGCTCATCAAGAAAATGCCAGCTGCGGCGACCATCCCGGCCAACAGCTCGGACGC
GCCCTTCATCCGGCCGGGACCCGGGACGCTGGATGGCCGCTGGGTCAGCCTGCACCGGAG
CCAGCAGGAGCGCAAGCGGGTGATGCAGGAGGCCTGCGCCAAGTACCGGGCGAGCAGCAG
CCGCCGGGCCGTCACGCCCCGCCACGTGTCCCGTATCTTCGTGGAGGACCGCCACCGCGT
GCTCTACTGCGAGGTGCCCAAGGCCGGCTGCTCCAATTGGAAGCGGGTGCTCATGGTGCT
GGCCGGCCTGGCCTCGTCCACTGCCGACATCCAGCACAACACCGTCCACTATGGCAGCGC
TCTCAAGCGCCTGGACACCTTCGACCGCCAGGGTATCTTGCACCGTCTCAGCACCTACAC
CAAGATGCTCTTTGTCCGCGAGCCCTTCGAGAGGCTGGTGTCCGCCTTCCGCGACAAGTT
TGAGCACCCCAACAGCTACTATCACCCGGTCTTCGGCAAGGCCATCCTGGCCCGGTACCG
CGCCAATGCCTCTCGGGAGGCCCTGCGGACCGGCTCTGGGGTGCGTTTTCCCGAGTTCGT
CCAGTACCTGCTGGACGTGCACCGGCCCGTGGGGATGGACATTCACTGGGACCATGTCAG
CCGGCTCTGCAGCCCCTGCCTCATCGACTACGATTTCGTAGGCAAGTTCGAGAGCATGGA
GGACGATGCCAACTTCTTCCTGAGCCTCATCCGCGCGCCGCGGAACCTGACCTTCCCCCG
GTTCAAGGACCGGCACTCGCAGGAGGCGCGGACCACAGCGAGGATCGCCCACCAGTACTT
CGCCCAACTCTCGGCCCTGCAAAGGCAGCGCACCTACGACTTCTACTACATGGATTACCT
GATGTTCAACTATTCCAAGCCCTTTGCAGATCTGTACGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT