FBXW8 ORF clone pFN21AE4158

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4158

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Clone info.

Species

human

Clone Name

pFN21AE4158 Sequence (with vector) Restriction Map

Product ID

FHC24900

ORF Sequence

DNA sequence (1596 bp)
Protein sequence (532 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4158

Symbol

FBXW8

Alias : FBW6, FBW8, FBX29, FBXO29, FBXW6

Description

F-box and WD repeat domain containing 8, transcript variant 2

Original Clone ID

cp94158

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1596 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 532 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44988.1 1.5e-186 99.8 FBXW8 CCDS9182.1 3.8e-172 94.5 FBXW8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001810 52 93 PF00646 F-box domain IPR019781 315 395 PF00400 WD40 repeat IPR019781 404 435 PF00400 WD40 repeat HMMSmart IPR001810 53 93 SM00256 F-box domain IPR001680 132 170 SM00320 WD40 repeat IPR001680 173 219 SM00320 WD40 repeat IPR001680 223 260 SM00320 WD40 repeat IPR001680 359 395 SM00320 WD40 repeat IPR001680 398 435 SM00320 WD40 repeat ProfileScan IPR001810 47 93 PS50181 F-box domain IPR017986 138 252 PS50294 WD40-repeat-containing domain IPR017986 362 444 PS50294 WD40-repeat-containing domain IPR019782 405 444 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036306 532 609073 99.8 100.0 NP_699179 598 609073 94.5 98.3 XP_016874665 599 609073 99.8 92.7 XP_016874664 517 609073 99.6 92.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4158 Download

>KIEE4158  1596 bp
ATGGACGACTACAGCCTGGATGAGTTCCGTCGGCGCTGGCAGGAGGAGCTGGCGCAGGCC
CAGGCGCCGAAGAAGCGGCGACGGCCCGAGGCTGCCGAGAGGCGGGCTCGGCGGCCGGAG
AATGAAATGAATGATGTGCCTTTCTTTGATATCCAACTGCCTTACGAATTGGCAATCAAT
ATATTTCAGTATCTGGACAGGAAAGAACTAGGAAGATGTGCACAGGTGAGCAAGACGTGG
AAGGTGATTGCAGAGGATGAGGTGCTGTGGTACAGGCTGTGCCAGCAGGAAGGGCACCTT
CCGGATAGCAGCATCTCTGACTATTCTTGCTGGAAGCTCATCTTCCAAGAGTGCCGAGCC
AAGGAACACATGTTACAAACCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGGAG
CATGTTCCTGACACAGTTTTGTGTGATGTGCATTCTCACGATGGTGTGGTCATTGCGGGA
TATACATCAGGGGATGTGAGAGTGTGGGACACCCGCACCTGGGACTACGTAGCCCCCTTC
CTGGAATCAGAGGACGAGGAGGATGAGCCTGGAATGCAGCCAAATGTCTCCTTTGTGAGG
ATAAACAGCTCGTTGGCAGTAGCAGCTTATGAGGATGGGTTTCTTAATATTTGGGATTTA
AGGACCGGAAAGTACCCTGTTCATCGTTTTGAGCACGATGCAAGAATACAGGCACTAGCC
CTCAGCCAGGACGATGCAACCGTGGCCACAGCTTCTGCTTTTGATGTCGTGATGTTATCC
CCCAATGAGGAGGGGTACTGGCAGATAGCTGCGGAATTTGAAGTTCCGAAACTGGTTCAG
TACCTTGAAATAGTTCCAGAAACCAGAAGGTACCCTGTGGCAGTAGCCGCTGCTGGAGAT
CTGATGTACCTGCTCAAAGCCGAAGACTCCGCCAGAACCCTCCTTTACGCCCACGGCCCG
CCTGTCACATGTCTAGACGTCTCGGCCAACCAAGTTGCTTTTGGTGTACAGGGTCTGGGA
TGGGTGTACGAAGGAAGCAAGATCCTGGTGTATAGCCTGGAAGCAGGACGCCGCCTCTTG
AAGCTGGGTAACGTTCTCCGTGACTTCACGTGTGTCAACCTCAGCGACAGCCCTCCCAAC
CTCATGGTCAGTGGCAACATGGACGGGAGGGTGAGGATCCACGACCTCCGCAGTGGTAAC
ATCGCCCTGTCGCTCTCCGCCCATCAGCTCAGGGTCTCTGCTGTGCAGATGGATGACTGG
AAGATCGTCAGTGGAGGCGAGGAAGGCCTGGTGTCCGTGTGGGATTATCGGATGAACCAG
AAGCTGTGGGAGGTGTATTCCGGGCACCCGGTGCAGCACATCTCATTCAGCAGCCACAGC
CTCATCACGGCCAACGTGCCTTACCAGACGGTAATGCGAAACGCCGACCTGGACAGCTTC
ACTACTCACAGGAGACACCGGGGGCTGATCCGCGCCTATGAGTTTGCGGTGGACCAGCTG
GCCTTCCAGAGCCCTCTCCCTGTCTGCCGTTCATCCTGTGACGCCATGGCCACTCACTAC
TACGACCTCGCACTGGCCTTTCCCTATAACCATGTT

ORF Protein

Cloned ORF protein sequence for pFN21AE4158 Download

>KIEE4158  532 aa
MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPENEMNDVPFFDIQLPYELAIN
IFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRA
KEHMLQTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPF
LESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEHDARIQALA
LSQDDATVATASAFDVVMLSPNEEGYWQIAAEFEVPKLVQYLEIVPETRRYPVAVAAAGD
LMYLLKAEDSARTLLYAHGPPVTCLDVSANQVAFGVQGLGWVYEGSKILVYSLEAGRRLL
KLGNVLRDFTCVNLSDSPPNLMVSGNMDGRVRIHDLRSGNIALSLSAHQLRVSAVQMDDW
KIVSGGEEGLVSVWDYRMNQKLWEVYSGHPVQHISFSSHSLITANVPYQTVMRNADLDSF
TTHRRHRGLIRAYEFAVDQLAFQSPLPVCRSSCDAMATHYYDLALAFPYNHV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4158 Download

>pFN21AE4158  6301 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGACTACAGCCTGGATGAGTTCCGTCGGCG
CTGGCAGGAGGAGCTGGCGCAGGCCCAGGCGCCGAAGAAGCGGCGACGGCCCGAGGCTGC
CGAGAGGCGGGCTCGGCGGCCGGAGAATGAAATGAATGATGTGCCTTTCTTTGATATCCA
ACTGCCTTACGAATTGGCAATCAATATATTTCAGTATCTGGACAGGAAAGAACTAGGAAG
ATGTGCACAGGTGAGCAAGACGTGGAAGGTGATTGCAGAGGATGAGGTGCTGTGGTACAG
GCTGTGCCAGCAGGAAGGGCACCTTCCGGATAGCAGCATCTCTGACTATTCTTGCTGGAA
GCTCATCTTCCAAGAGTGCCGAGCCAAGGAACACATGTTACAAACCAACTGGAAGAATCG
CAAAGGTGCCGTGAGCGAGCTGGAGCATGTTCCTGACACAGTTTTGTGTGATGTGCATTC
TCACGATGGTGTGGTCATTGCGGGATATACATCAGGGGATGTGAGAGTGTGGGACACCCG
CACCTGGGACTACGTAGCCCCCTTCCTGGAATCAGAGGACGAGGAGGATGAGCCTGGAAT
GCAGCCAAATGTCTCCTTTGTGAGGATAAACAGCTCGTTGGCAGTAGCAGCTTATGAGGA
TGGGTTTCTTAATATTTGGGATTTAAGGACCGGAAAGTACCCTGTTCATCGTTTTGAGCA
CGATGCAAGAATACAGGCACTAGCCCTCAGCCAGGACGATGCAACCGTGGCCACAGCTTC
TGCTTTTGATGTCGTGATGTTATCCCCCAATGAGGAGGGGTACTGGCAGATAGCTGCGGA
ATTTGAAGTTCCGAAACTGGTTCAGTACCTTGAAATAGTTCCAGAAACCAGAAGGTACCC
TGTGGCAGTAGCCGCTGCTGGAGATCTGATGTACCTGCTCAAAGCCGAAGACTCCGCCAG
AACCCTCCTTTACGCCCACGGCCCGCCTGTCACATGTCTAGACGTCTCGGCCAACCAAGT
TGCTTTTGGTGTACAGGGTCTGGGATGGGTGTACGAAGGAAGCAAGATCCTGGTGTATAG
CCTGGAAGCAGGACGCCGCCTCTTGAAGCTGGGTAACGTTCTCCGTGACTTCACGTGTGT
CAACCTCAGCGACAGCCCTCCCAACCTCATGGTCAGTGGCAACATGGACGGGAGGGTGAG
GATCCACGACCTCCGCAGTGGTAACATCGCCCTGTCGCTCTCCGCCCATCAGCTCAGGGT
CTCTGCTGTGCAGATGGATGACTGGAAGATCGTCAGTGGAGGCGAGGAAGGCCTGGTGTC
CGTGTGGGATTATCGGATGAACCAGAAGCTGTGGGAGGTGTATTCCGGGCACCCGGTGCA
GCACATCTCATTCAGCAGCCACAGCCTCATCACGGCCAACGTGCCTTACCAGACGGTAAT
GCGAAACGCCGACCTGGACAGCTTCACTACTCACAGGAGACACCGGGGGCTGATCCGCGC
CTATGAGTTTGCGGTGGACCAGCTGGCCTTCCAGAGCCCTCTCCCTGTCTGCCGTTCATC
CTGTGACGCCATGGCCACTCACTACTACGACCTCGCACTGGCCTTTCCCTATAACCATGT
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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