ANAPC5 ORF clone pFN21AE3767

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3767

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Clone info.

Species

human

Clone Name

pFN21AE3767 Sequence (with vector) Restriction Map

Product ID

FHC11084

ORF Sequence

DNA sequence (2265 bp)
Protein sequence (755 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3767

Symbol

ANAPC5

Alias : APC5

Description

anaphase promoting complex subunit 5, transcript variant 1

Original Clone ID

cp93767

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2265 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 755 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9220.1 0 100.0 ANAPC5 CCDS81749.1 6.6e-145 98.3 ANAPC5 CCDS45000.1 1e-141 98.0 ANAPC5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMSmart IPR019734 301 334 SM00028 Tetratricopeptide repeat IPR019734 467 500 SM00028 Tetratricopeptide repeat IPR019734 541 574 SM00028 Tetratricopeptide repeat IPR019734 581 614 SM00028 Tetratricopeptide repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057321 755 606948 100.0 100.0 NP_001317418 742 606948 98.3 100.0 NP_001131031 643 606948 98.0 86.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3767 Download

>KIEE3767  2265 bp
ATGGCCAGCGTCCACGAGAGCCTCTACTTCAATCCCATGATGACCAATGGGGTTGTGCAC
GCCAATGTGTTCGGCATCAAGGACTGGGTGACGCCGTACAAGATCGCGGTGCTGGTGCTG
CTGAACGAGATGAGCCGCACAGGCGAGGGCGCCGTCAGCCTCATGGAGCGGCGGAGGCTC
AACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAACTTTACAAG
TTAATTGAAGAGTCTTGTCCACAGCTGGCAAATTCAGTGCAGATCAGAATCAAACTGATG
GCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGATTCTTTCTCT
GGAACTGAACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATC
TTGGCCTACAGTAAGCTTTCTTTCAGCCAAGTGTTCAAACTGTACACTGCCCTTCAGCAG
TACTTCCAGAATGGTGAGAAAAAGACAGTGGAGGATGCTGATATGGAACTGACCAGTAGA
GATGAGGGTGAAAGAAAAATGGAAAAAGAAGAACTTGATGTATCTGTAAGAGAAGAGGAG
GTATCTTGCAGTGGGCCTCTGTCCCAAAAACAAGCAGAATTTTTTCTTTCTCAACAGGCT
TCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAGCTTCCTTGCAGAAGGAATTA
AACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAGCTACTTAAAC
AACCTCCGTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGT
CTGATTCTTACCGGAGCCGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTG
AGATACGCCGCTCTGAATCTTGCCGCCCTGCACTGCCGCTTCGGTCACTATCAACAGGCA
GAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTC
CAGCACTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGATCCGATAGCTATGTTCTG
CTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTACCGTACCTCGCCTCCCTGGGA
ATACAGTCCCTTGTTCAACAGAGAGCTTTTGCTGGGAAGACGGCAAACAAGCTGATGGAT
GCCCTAAAGGACTCCGACCTCCTGCACTGGAAACACAGCCTGTCAGAGCTCATCGATATC
AGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGCAGCACCATGGCACTGCAA
CAGGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGCGTGCAGCAG
AACAACACAGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAG
GGCTGTTTTGCTGCAGCTTCTGAAGTGTTAAAGCACTTGAAGGAACGATTTCCGCCTAAT
AGTCAGCACGCCCAGTTATGGATGCTATGTGATCAAAAAATACAGTTTGACAGAGCAATG
AATGATGGCAAATATCATTTGGCTGATTCACTTGTTACAGGAATCACAGCTCTCAATAGC
ATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCAGAACCAAATGTCAGAGGCA
CATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAGAAACTGAAGAACACAGAAATGGTG
ATCAGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCCCCTACCATCGCG
CTGCCCATGCTCCTGCAGGCTCTGGCCCTCTCCAAGGAGTACCGGTTACAGTACTTGGCC
TCTGAAACAGTGCTGAACTTGGCTTTTGCGCAGCTCATTCTTGGAATCCCAGAACAGGCC
TTAAGTCTTCTCCACATGGCCATCGAGCCCATCTTGGCTGACGGGGCTATCCTGGACAAA
GGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGTGGCTTCAGCAGCTTCCTACGATCAG
CCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGAGAACCTCAATGAAGCCAAGAACTAT
TTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGACGTCGTTTACTTCCAGGCCAGACTC
TACCATACCCTGGGGAAGACCCAGGAGAGGAACCGGTGTGCGATGCTCTTCCGGCAGCTG
CATCAGGAGCTGCCCTCTCATGGGGTACCCTTGATAAACCATCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE3767 Download

>KIEE3767  755 aa
MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRL
NQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFS
GTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSR
DEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKEL
NNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSL
RYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVL
LEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDI
SIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNNTESFAVALCHLAELHAEQ
GCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITALNS
IEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIA
LPMLLQALALSKEYRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDK
GRAMFLVAKCQVASAASYDQPKKAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARL
YHTLGKTQERNRCAMLFRQLHQELPSHGVPLINHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3767 Download

>pFN21AE3767  6970 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAGCGTCCACGAGAGCCTCTACTTCAATCC
CATGATGACCAATGGGGTTGTGCACGCCAATGTGTTCGGCATCAAGGACTGGGTGACGCC
GTACAAGATCGCGGTGCTGGTGCTGCTGAACGAGATGAGCCGCACAGGCGAGGGCGCCGT
CAGCCTCATGGAGCGGCGGAGGCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGA
TATTACACTGTCAAAACTTTACAAGTTAATTGAAGAGTCTTGTCCACAGCTGGCAAATTC
AGTGCAGATCAGAATCAAACTGATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTT
TGATGACCTTTCAGATTCTTTCTCTGGAACTGAACCAGAGGTTCACAAAACAAGTGTAGT
AGGTTTGTTTCTGCGTCACATGATCTTGGCCTACAGTAAGCTTTCTTTCAGCCAAGTGTT
CAAACTGTACACTGCCCTTCAGCAGTACTTCCAGAATGGTGAGAAAAAGACAGTGGAGGA
TGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAATGGAAAAAGAAGAACT
TGATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAACAAGC
AGAATTTTTTCTTTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCAC
TCCAGCTTCCTTGCAGAAGGAATTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGA
AGCGCATTATCTCAGCTACTTAAACAACCTCCGTGTCCAAGATGTTTTCAGTTCAACACA
CAGTCTCCTCCATTATTTTGATCGTCTGATTCTTACCGGAGCCGAAAGCAAAAGTAATGG
GGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCGCTCTGAATCTTGCCGCCCTGCACTG
CCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCA
GGAGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTGGGGCA
GAAGAGATCCGATAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGG
GTTACCGTACCTCGCCTCCCTGGGAATACAGTCCCTTGTTCAACAGAGAGCTTTTGCTGG
GAAGACGGCAAACAAGCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAACA
CAGCCTGTCAGAGCTCATCGATATCAGCATCGCACAGAAAACGGCCATCTGGAGGCTGTA
TGGCCGCAGCACCATGGCACTGCAACAGGCCCAGATGTTGCTGAGCATGAACAGCCTGGA
GGCGGTGAATGCGGGCGTGCAGCAGAACAACACAGAGTCCTTTGCTGTCGCACTCTGCCA
CCTCGCAGAGCTACACGCGGAGCAGGGCTGTTTTGCTGCAGCTTCTGAAGTGTTAAAGCA
CTTGAAGGAACGATTTCCGCCTAATAGTCAGCACGCCCAGTTATGGATGCTATGTGATCA
AAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATATCATTTGGCTGATTCACTTGT
TACAGGAATCACAGCTCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACA
AGCTCAGAACCAAATGTCAGAGGCACATAAGCTTTTACAAAAATTGTTGGTTCATTGTCA
GAAACTGAAGAACACAGAAATGGTGATCAGTGTCCTACTGTCCGTGGCAGAGCTGTACTG
GCGATCTTCCTCCCCTACCATCGCGCTGCCCATGCTCCTGCAGGCTCTGGCCCTCTCCAA
GGAGTACCGGTTACAGTACTTGGCCTCTGAAACAGTGCTGAACTTGGCTTTTGCGCAGCT
CATTCTTGGAATCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGAGCCCATCTT
GGCTGACGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGT
GGCTTCAGCAGCTTCCTACGATCAGCCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGA
GAACCTCAATGAAGCCAAGAACTATTTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGA
CGTCGTTTACTTCCAGGCCAGACTCTACCATACCCTGGGGAAGACCCAGGAGAGGAACCG
GTGTGCGATGCTCTTCCGGCAGCTGCATCAGGAGCTGCCCTCTCATGGGGTACCCTTGAT
AAACCATCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT