PIKFYVE ORF clone pFN21AE3719

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3719

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Clone info.

Species

human

Clone Name

pFN21AE3719 Sequence (with vector) Restriction Map

Product ID

FHC21300

ORF Sequence

DNA sequence (6294 bp)
Protein sequence (2098 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3719

Symbol

PIKFYVE

Alias : CFD, FAB1, HEL37, PIP5K, PIP5K3, ZFYVE29

Description

phosphoinositide kinase, FYVE finger containing, transcript variant 2

Original Clone ID

cs93719

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 6294 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 2098 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2382.1 0 100.0 PIKFYVE CCDS54431.1 5.5e-184 100.0 PIKFYVE CCDS33368.1 7e-111 82.2 PIKFYVE CCDS30888.1 4.2e-12 25.5 CCT3 CCDS1140.2 4.5e-12 25.5 CCT3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000306 154 215 PF01363 Zinc finger IPR000591 368 437 PF00610 DEP domain IPR002423 608 863 PF00118 Chaperonin Cpn60/TCP-1 IPR002498 1853 2084 PF01504 Phosphatidylinositol-4-phosphate 5-kinase HMMSmart IPR000306 150 219 SM00064 Zinc finger IPR000591 365 440 SM00049 DEP domain IPR016034 1791 2085 SM00330 Phosphatidylinositol-4-phosphate 5-kinase ProfileScan IPR017455 158 218 PS50178 Zinc finger IPR000591 365 440 PS50186 DEP domain IPR002498 1758 2084 PS51455 Phosphatidylinositol-4-phosphate 5-kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055855 2098 121850,609414 100.0 100.0 XP_016859057 2092 121850,609414 99.7 100.0 XP_011509083 2104 121850,609414 99.1 100.0 XP_011509082 2109 121850,609414 99.5 100.0 XP_011509080 2110 121850,609414 99.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3719 Download

>KIEE3719  6294 bp
ATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAATGATTTGCCTCGATCT
CCTACTAGTCCTTCTCATCTCACACACTTTAAACCTTTGACTCCTGATCAAGATGAGCCC
CCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCTCTTTCGTTTTAACAAAGAGAGAGCA
GAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGGAAGTTGGACCAGCCCTCAGCTCCCT
TCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAAAGCAGCTTAATGAGGAACTC
CAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCTACCTTTGGAGGTCAT
GACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTAAAACGCCTCAAGGAA
ATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAATACTGGATGCCAGATAGCCAA
TGTAAAGAGTGCTATGACTGTAGTGAGAAATTTACAACCTTTAGGCGCAGACACCATTGC
CGACTATGTGGGCAGATTTTCTGCAGTCGTTGCTGTAATCAAGAAATCCCTGGAAAATTT
ATGGGCTATACAGGAGACCTCCGAGCTTGCACATATTGTAGAAAAATAGCCTTAAGTTAT
GCTCATTCCACAGACAGTAATTCTATTGGGGAAGACTTGAATGCTCTTTCAGATTCTGCT
TGCTCTGTGTCTGTGCTTGATCCAAGTGAACCCCGAACACCTGTTGGGAGTAGGAAAGCC
AGCCGTAACATATTTTTAGAGGATGATTTGGCCTGGCAAAGTTTGATTCATCCAGATTCC
TCAAATACTCCTCTTTCAACAAGACTTGTATCTGTGCAAGAGGATGCTGGGAAATCTCCT
GCTCGAAATAGATCAGCCAGCATTACTAACCTGTCACTGGATAGATCTGGTTCTCCTATG
GTACCTTCATATGAGACATCTGTCAGTCCCCAGGCTAACCGAACATATGTTAGGACAGAG
ACCACTGAGGATGAACGCAAAATTCTTCTGGACAGTGTGCAGTTAAAAGACCTGTGGAAA
AAAATCTGCCATCACAGCAGTGGAATGGAGTTTCAGGATCACCGCTACTGGTTGAGAACG
CATCCCAACTGCATTGTAGGAAAGGAATTAGTCAACTGGCTAATCCGAAATGGGCATATT
GCCACAAGGGCACAAGCTATAGCAATTGGACAAGCAATGGTTGATGGACGTTGGCTGGAT
TGTGTTAGTCATCACGACCAGCTTTTCAGAGATGAGTATGCGCTGTATAGACCACTGCAG
AGTACAGAATTTTCTGAGACGCCTTCTCCCGACAGTGACTCAGTGAACTCCGTGGAAGGA
CACTCTGAGCCATCCTGGTTTAAAGACATAAAGTTTGATGACAGTGACACAGAACAGATA
GCTGAAGAAGGTGACGATAATTTGGCTAATTCTGCCAGTCCTAGCAAGCGCACATCAGTC
AGCAGTTTCCAGTCCACAGTGGACAGTGACTCAGCCGCTTCTATCAGCCTGAACGTGGAG
CTGGACAACGTGAACTTCCATATCAAGAAGCCCTCCAAGTACCCACATGTGCCCCCTCAC
CCTGCTGACCAAAAAGAGTATTTGATTTCTGACACTGGAGGACAACAGCTCTCAATAAGT
GACGCTTTCATCAAAGAATCCTTATTTAATCGCCGAGTAGAGGAAAAATCCAAAGAGCTG
CCTTTCACACCTTTGGGCTGGCATCATAACAACCTGGAGCTCCTGAGGGAGGAGAATGGG
GAGAAACAAGCCATGGAGAGGTTGCTTTCAGCTAATCATAACCACATGATGGCACTACTC
CAGCAGTTGCTCCATAGTGACTCACTGTCATCATCTTGGAGGGACATCATCGTGTCATTG
GTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGTCAAGAACCAGGATGATGACATGGAT
ATCCGTCAGTTTGTCCACATCAAAAAAATCCCAGGTGGAAAGAAGTTTGATTCTGTGGTT
GTCAATGGCTTTGTTTGTACCAAGAACATTGCACATAAAAAGATGAGTTCTTGTATTAAA
AACCCCAAAATTCTTCTGTTGAAGTGTTCCATTGAGTATCTCTACAGAGAAGAAACTAAG
TTTACTTGCATTGATCCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTCCAG
CGAATAGTTGATGTTCGACCCACCTTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCC
CAGGACATGTTATTGGAACATGGCATTACTTTGGTCATTAATGTAAAGTCACAAGTTTTG
GAACGAATCAGTCGAATGACCCAAGGTGATTTAGTGATGTCAATGGACCAGCTGCTTACG
AAACCACACCTGGGCACTTGTCACAAATTTTATATGCAGATATTTCAGTTGCCTAATGAA
CAAACCAAGACACTGATGTTTTTTGAAGGTTGTCCACAGCACCTAGGCTGTACAATCAAG
CTAAGAGGAGGCTCTGATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATC
TGTGTTGCTTATCATTCTCAACTAGAAATATCCTTTCTCATGGATGAATTTGCTATGCCT
CCCACATTAATGCAAAACCCTTCATTCCATTCCCTGATTGAGGGACGAGGGCATGAGGGG
GCTGTCCAAGAGCAGTACGGTGGAGGTTCCATCCCCTGGGATCCTGACATCCCTCCTGAG
TCTCTGCCCTGTGATGATAGCAGTTTGCTGGAATTGAGGATTGTGTTTGAGAAGGGTGAG
CAGGAAAATAAAAATCTTCCGCAGGCTGTTGCCTCTGTGAAGCATCAAGAACATAGCACA
ACAGCTTGCCCGGCGGGTCTCCCTTGTGCTTTCTTTGCACCTGTACCGGAATCATTGTTG
CCACTCCCTGTGGATGACCAACAAGATGCTTTAGGCAGCGAGCAGCCAGAGACTTTGCAG
CAAACAGTTGTGCTGCAGGATCCCAAAAGCCAGATAAGAGCCTTTAGAGACCCTCTACAG
GATGACACTGGATTATATGTTACTGAGGAAGTCACCTCCTCTGAAGATAAACGAAAGACT
TATTCTTTGGCCTTTAAGCAGGAATTAAAAGATGTGATCCTCTGTATCTCCCCAGTAATC
ACATTCCGAGAACCCTTTCTTTTAACTGAAAAGGGGATGAGATGCTCTACCCGAGATTAT
TTTGCAGAGCAGGTTTACTGGTCTCCTCTCCTCAATAAAGAATTCAAAGAAATGGAGAAC
AGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGAAGTATT
CAGGCCAAGTCTATTCAAGTCTTACCCTCACATGAGCTAGTGAGCACTAGAATTGCTGAG
CATCTGGGCGATAGCCAGAGCTTGGGTAGAATGCTGGCCGATTATCGAGCCAGAGGAGGA
AGAATTCAGCCCAAAAATTCAGACCCTTTTGCTCATTCAAAGGATGCATCAAGTACTTCA
AGTGGCCAATCAGGAAGCAAAAATGAGGGTGATGAAGAGAGAGGGCTTATTCTGAGTGAT
GCTGTGTGGTCAACAAAGGTGGACTGTCTGAATCCCATTAATCACCAGAGACTTTGTGTG
CTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGCTCCTAGTGCCTGTGTCAGTCCTTGG
ATTGTAACAATGGAATTTTATGGAAAGAATGATCTTACATTAGGAATATTTTTAGAGAGA
TACTGTTTCAGGCCTTCTTATCAGTGTCCAAGCATGTTCTGTGATACCCCCATGGTACAT
CATATTCGGCGCTTTGTTCATGGCCAAGGCTGTGTGCAGATAATCCTGAAGGAGTTGGAT
TCTCCAGTACCTGGATATCAGCATACAATTCTTACATATTCCTGGTGTAGAATCTGCAAA
CAGGTAACACCAGTTGTTGCTCTTTCCAATGAGTCCTGGTCTATGTCATTTGCAAAATAC
CTTGAACTTAGGTTTTATGGGCACCAGTATACTCGCAGAGCCAACGCTGAGCCCTGTGGT
CACTCCATCCATCATGATTATCACCAGTATTTCTCCTATAACCAGATGGTGGCGTCTTTC
AGTTATTCTCCCATTCGGCTTCTTGAAGTATGTGTTCCACTCCCCAAAATATTCATTAAG
CGTCAGGCCCCATTAAAAGTGTCCCTTCTTCAGGATCTGAAGGACTTCTTTCAAAAAGTT
TCACAGGTATATGTTGCCATTGATGAAAGACTTGCATCTTTGAAAACTGATACATTTAGT
AAAACAAGAGAGGAAAAAATGGAAGATATTTTTGCACAGAAAGAGATGGAAGAAGGTGAG
TTCAAGAACTGGATTGAGAAGATGCAAGCAAGGCTCATGTCTTCCTCTGTAGATACCCCT
CAGCAACTGCAGTCGGTCTTTGAGTCACTCATTGCCAAGAAACAAAGTCTCTGTGAAGTG
CTGCAAGCTTGGAATAACAGGTTGCAGGACCTTTTCCAACAGGAAAAGGGTAGAAAGAGA
CCTTCAGTTCCTCCAAGTCCTGGAAGACTGAGACAAGGGGAAGAAAGCAAGATAAGTGCG
ATGGATGCATCTCCACGGAATATTTCTCCAGGACTTCAGAATGGAGAAAAAGAGGATCGC
TTCTTAACAACTTTGTCCAGCCAGAGCTCCACCAGTTCTACTCATCTCCAATTGCCTACG
CCACCTGAAGTCATGTCTGAACAGTCAGTGGGAGGGCCCCCTGAGCTAGATACAGCCAGC
AGTTCCGAAGATGTGTTTGATGGGCATTTGCTGGGATCCACAGACAGCCAAGTGAAGGAA
AAGTCAACCATGAAAGCCATCTTTGCAAATTTGCTTCCAGGAAATAGCTATAATCCTATT
CCATTTCCTTTTGATCCAGATAAACACTACTTAATGTATGAACATGAACGAGTGCCCATT
GCAGTCTGCGAGAAGGAACCCAGCTCCATCATTGCTTTTGCTCTCAGTTGTAAAGAATAC
CGAAATGCCTTAGAGGAATTGTCTAAAGCGACTCAGTGGAACAGTGCCGAAGAAGGGCTT
CCAACAAATAGTACTTCAGATAGCAGACCAAAGAGTAGCAGCCCTATCAGATTACCTGAA
ATGAGTGGAGGACAGACAAATCGTACAACAGAAACAGAACCACAACCAACCAAAAAGGCT
TCTGGAATGTTGTCCTTCTTCAGAGGGACAGCAGGGAAAAGCCCCGATCTCTCTTCCCAG
AAGAGAGAGACCTTACGTGGAGCAGATAGTGCTTACTACCAGGTTGGGCAGACGGGCAAG
GAGGGGACCGAGAATCAAGGCGTTGAGCCTCAAGATGAAGTAGATGGAGGAGATACACAA
AAGAAGCAACTCATAAATCCTCATGTGGAACTTCAATTTTCAGATGCTAATGCCAAGTTT
TACTGTCGGCTCTACTATGCGGGAGAGTTTCATAAGATGCGTGAAGTGATTCTGGACAGC
AGTGAAGAAGATTTCATTCGTTCCCTCTCCCACTCATCACCCTGGCAGGCCCGGGGAGGC
AAATCAGGAGCTGCCTTCTATGCAACTGAGGATGATAGATTTATTTTGAAGCAAATGCCT
CGTCTGGAAGTCCAGTCCTTCCTCGACTTTGCACCACATTACTTCAATTATATTACAAAT
GCTGTTCAACAAAAGAGGCCCACGGCGTTGGCCAAAATTCTTGGAGTTTACAGAATTGGT
TATAAGAACTCTCAGAACAACACTGAGAAGAAGTTAGATCTCCTTGTCATGGAAAATCTT
TTCTACGGGAGAAAGATGGCACAGGTTTTTGATTTGAAGGGCTCTCTTAGGAATCGGAAT
GTAAAAACTGACACTGGAAAAGAGAGTTGTGATGTGGTCCTGCTAGATGAAAATCTCCTA
AAGATGGTTCGAGACAACCCTCTATATATTCGTTCTCATTCCAAAGCTGTGCTGAGAACC
TCGATCCATAGTGACTCCCATTTCCTTTCTAGCCACCTCATTATAGATTATTCTTTGCTG
GTTGGGCGAGATGATACTAGCAATGAGCTAGTAGTTGGAATTATAGATTATATTCGAACA
TTTACATGGGACAAAAAGCTTGAGATGGTTGTGAAATCAACAGGAATTTTAGGTGGACAA
GGTAAAATGCCAACAGTGGTGTCTCCGGAGTTGTACAGGACTAGGTTTTGTGAGGCAATG
GACAAGTATTTCCTAATGGTACCAGACCACTGGACAGGCTTGGGTCTGAATTGC

ORF Protein

Cloned ORF protein sequence for pFN21AE3719 Download

>KIEE3719  2098 aa
MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERA
EGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGH
DPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHC
RLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSA
CSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSP
ARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWK
KICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLD
CVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQI
AEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPH
PADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENG
EKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMD
IRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETK
FTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVL
ERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIK
LRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEG
AVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHST
TACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQ
DDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDY
FAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAE
HLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSD
AVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLER
YCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICK
QVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASF
SYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFS
KTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEV
LQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDR
FLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKE
KSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEY
RNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKA
SGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQ
KKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGG
KSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIG
YKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLL
KMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRT
FTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3719 Download

>pFN21AE3719  10999 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCACAGATGATAAGACGTCCCCAACACTGGA
CTCTGCTAATGATTTGCCTCGATCTCCTACTAGTCCTTCTCATCTCACACACTTTAAACC
TTTGACTCCTGATCAAGATGAGCCCCCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCT
CTTTCGTTTTAACAAAGAGAGAGCAGAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGG
AAGTTGGACCAGCCCTCAGCTCCCTTCGAGGACACAGTCTGTTAGGTCACCCACACCTTA
TAAAAAGCAGCTTAATGAGGAACTCCAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAA
AGCAGAACCTACCTTTGGAGGTCATGACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAG
CACAGTATTAAAACGCCTCAAGGAAATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAA
ACAATACTGGATGCCAGATAGCCAATGTAAAGAGTGCTATGACTGTAGTGAGAAATTTAC
AACCTTTAGGCGCAGACACCATTGCCGACTATGTGGGCAGATTTTCTGCAGTCGTTGCTG
TAATCAAGAAATCCCTGGAAAATTTATGGGCTATACAGGAGACCTCCGAGCTTGCACATA
TTGTAGAAAAATAGCCTTAAGTTATGCTCATTCCACAGACAGTAATTCTATTGGGGAAGA
CTTGAATGCTCTTTCAGATTCTGCTTGCTCTGTGTCTGTGCTTGATCCAAGTGAACCCCG
AACACCTGTTGGGAGTAGGAAAGCCAGCCGTAACATATTTTTAGAGGATGATTTGGCCTG
GCAAAGTTTGATTCATCCAGATTCCTCAAATACTCCTCTTTCAACAAGACTTGTATCTGT
GCAAGAGGATGCTGGGAAATCTCCTGCTCGAAATAGATCAGCCAGCATTACTAACCTGTC
ACTGGATAGATCTGGTTCTCCTATGGTACCTTCATATGAGACATCTGTCAGTCCCCAGGC
TAACCGAACATATGTTAGGACAGAGACCACTGAGGATGAACGCAAAATTCTTCTGGACAG
TGTGCAGTTAAAAGACCTGTGGAAAAAAATCTGCCATCACAGCAGTGGAATGGAGTTTCA
GGATCACCGCTACTGGTTGAGAACGCATCCCAACTGCATTGTAGGAAAGGAATTAGTCAA
CTGGCTAATCCGAAATGGGCATATTGCCACAAGGGCACAAGCTATAGCAATTGGACAAGC
AATGGTTGATGGACGTTGGCTGGATTGTGTTAGTCATCACGACCAGCTTTTCAGAGATGA
GTATGCGCTGTATAGACCACTGCAGAGTACAGAATTTTCTGAGACGCCTTCTCCCGACAG
TGACTCAGTGAACTCCGTGGAAGGACACTCTGAGCCATCCTGGTTTAAAGACATAAAGTT
TGATGACAGTGACACAGAACAGATAGCTGAAGAAGGTGACGATAATTTGGCTAATTCTGC
CAGTCCTAGCAAGCGCACATCAGTCAGCAGTTTCCAGTCCACAGTGGACAGTGACTCAGC
CGCTTCTATCAGCCTGAACGTGGAGCTGGACAACGTGAACTTCCATATCAAGAAGCCCTC
CAAGTACCCACATGTGCCCCCTCACCCTGCTGACCAAAAAGAGTATTTGATTTCTGACAC
TGGAGGACAACAGCTCTCAATAAGTGACGCTTTCATCAAAGAATCCTTATTTAATCGCCG
AGTAGAGGAAAAATCCAAAGAGCTGCCTTTCACACCTTTGGGCTGGCATCATAACAACCT
GGAGCTCCTGAGGGAGGAGAATGGGGAGAAACAAGCCATGGAGAGGTTGCTTTCAGCTAA
TCATAACCACATGATGGCACTACTCCAGCAGTTGCTCCATAGTGACTCACTGTCATCATC
TTGGAGGGACATCATCGTGTCATTGGTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGT
CAAGAACCAGGATGATGACATGGATATCCGTCAGTTTGTCCACATCAAAAAAATCCCAGG
TGGAAAGAAGTTTGATTCTGTGGTTGTCAATGGCTTTGTTTGTACCAAGAACATTGCACA
TAAAAAGATGAGTTCTTGTATTAAAAACCCCAAAATTCTTCTGTTGAAGTGTTCCATTGA
GTATCTCTACAGAGAAGAAACTAAGTTTACTTGCATTGATCCTATTGTGCTTCAGGAAAG
GGAATTCTTGAAGAATTATGTCCAGCGAATAGTTGATGTTCGACCCACCTTGGTTCTTGT
TGAGAAAACAGTGTCTCGGATTGCCCAGGACATGTTATTGGAACATGGCATTACTTTGGT
CATTAATGTAAAGTCACAAGTTTTGGAACGAATCAGTCGAATGACCCAAGGTGATTTAGT
GATGTCAATGGACCAGCTGCTTACGAAACCACACCTGGGCACTTGTCACAAATTTTATAT
GCAGATATTTCAGTTGCCTAATGAACAAACCAAGACACTGATGTTTTTTGAAGGTTGTCC
ACAGCACCTAGGCTGTACAATCAAGCTAAGAGGAGGCTCTGATTATGAGCTGGCTCGAGT
TAAGGAGATCCTAATATTTATGATCTGTGTTGCTTATCATTCTCAACTAGAAATATCCTT
TCTCATGGATGAATTTGCTATGCCTCCCACATTAATGCAAAACCCTTCATTCCATTCCCT
GATTGAGGGACGAGGGCATGAGGGGGCTGTCCAAGAGCAGTACGGTGGAGGTTCCATCCC
CTGGGATCCTGACATCCCTCCTGAGTCTCTGCCCTGTGATGATAGCAGTTTGCTGGAATT
GAGGATTGTGTTTGAGAAGGGTGAGCAGGAAAATAAAAATCTTCCGCAGGCTGTTGCCTC
TGTGAAGCATCAAGAACATAGCACAACAGCTTGCCCGGCGGGTCTCCCTTGTGCTTTCTT
TGCACCTGTACCGGAATCATTGTTGCCACTCCCTGTGGATGACCAACAAGATGCTTTAGG
CAGCGAGCAGCCAGAGACTTTGCAGCAAACAGTTGTGCTGCAGGATCCCAAAAGCCAGAT
AAGAGCCTTTAGAGACCCTCTACAGGATGACACTGGATTATATGTTACTGAGGAAGTCAC
CTCCTCTGAAGATAAACGAAAGACTTATTCTTTGGCCTTTAAGCAGGAATTAAAAGATGT
GATCCTCTGTATCTCCCCAGTAATCACATTCCGAGAACCCTTTCTTTTAACTGAAAAGGG
GATGAGATGCTCTACCCGAGATTATTTTGCAGAGCAGGTTTACTGGTCTCCTCTCCTCAA
TAAAGAATTCAAAGAAATGGAGAACAGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGG
ACTTCAGGGCATGAATGGAAGTATTCAGGCCAAGTCTATTCAAGTCTTACCCTCACATGA
GCTAGTGAGCACTAGAATTGCTGAGCATCTGGGCGATAGCCAGAGCTTGGGTAGAATGCT
GGCCGATTATCGAGCCAGAGGAGGAAGAATTCAGCCCAAAAATTCAGACCCTTTTGCTCA
TTCAAAGGATGCATCAAGTACTTCAAGTGGCCAATCAGGAAGCAAAAATGAGGGTGATGA
AGAGAGAGGGCTTATTCTGAGTGATGCTGTGTGGTCAACAAAGGTGGACTGTCTGAATCC
CATTAATCACCAGAGACTTTGTGTGCTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGC
TCCTAGTGCCTGTGTCAGTCCTTGGATTGTAACAATGGAATTTTATGGAAAGAATGATCT
TACATTAGGAATATTTTTAGAGAGATACTGTTTCAGGCCTTCTTATCAGTGTCCAAGCAT
GTTCTGTGATACCCCCATGGTACATCATATTCGGCGCTTTGTTCATGGCCAAGGCTGTGT
GCAGATAATCCTGAAGGAGTTGGATTCTCCAGTACCTGGATATCAGCATACAATTCTTAC
ATATTCCTGGTGTAGAATCTGCAAACAGGTAACACCAGTTGTTGCTCTTTCCAATGAGTC
CTGGTCTATGTCATTTGCAAAATACCTTGAACTTAGGTTTTATGGGCACCAGTATACTCG
CAGAGCCAACGCTGAGCCCTGTGGTCACTCCATCCATCATGATTATCACCAGTATTTCTC
CTATAACCAGATGGTGGCGTCTTTCAGTTATTCTCCCATTCGGCTTCTTGAAGTATGTGT
TCCACTCCCCAAAATATTCATTAAGCGTCAGGCCCCATTAAAAGTGTCCCTTCTTCAGGA
TCTGAAGGACTTCTTTCAAAAAGTTTCACAGGTATATGTTGCCATTGATGAAAGACTTGC
ATCTTTGAAAACTGATACATTTAGTAAAACAAGAGAGGAAAAAATGGAAGATATTTTTGC
ACAGAAAGAGATGGAAGAAGGTGAGTTCAAGAACTGGATTGAGAAGATGCAAGCAAGGCT
CATGTCTTCCTCTGTAGATACCCCTCAGCAACTGCAGTCGGTCTTTGAGTCACTCATTGC
CAAGAAACAAAGTCTCTGTGAAGTGCTGCAAGCTTGGAATAACAGGTTGCAGGACCTTTT
CCAACAGGAAAAGGGTAGAAAGAGACCTTCAGTTCCTCCAAGTCCTGGAAGACTGAGACA
AGGGGAAGAAAGCAAGATAAGTGCGATGGATGCATCTCCACGGAATATTTCTCCAGGACT
TCAGAATGGAGAAAAAGAGGATCGCTTCTTAACAACTTTGTCCAGCCAGAGCTCCACCAG
TTCTACTCATCTCCAATTGCCTACGCCACCTGAAGTCATGTCTGAACAGTCAGTGGGAGG
GCCCCCTGAGCTAGATACAGCCAGCAGTTCCGAAGATGTGTTTGATGGGCATTTGCTGGG
ATCCACAGACAGCCAAGTGAAGGAAAAGTCAACCATGAAAGCCATCTTTGCAAATTTGCT
TCCAGGAAATAGCTATAATCCTATTCCATTTCCTTTTGATCCAGATAAACACTACTTAAT
GTATGAACATGAACGAGTGCCCATTGCAGTCTGCGAGAAGGAACCCAGCTCCATCATTGC
TTTTGCTCTCAGTTGTAAAGAATACCGAAATGCCTTAGAGGAATTGTCTAAAGCGACTCA
GTGGAACAGTGCCGAAGAAGGGCTTCCAACAAATAGTACTTCAGATAGCAGACCAAAGAG
TAGCAGCCCTATCAGATTACCTGAAATGAGTGGAGGACAGACAAATCGTACAACAGAAAC
AGAACCACAACCAACCAAAAAGGCTTCTGGAATGTTGTCCTTCTTCAGAGGGACAGCAGG
GAAAAGCCCCGATCTCTCTTCCCAGAAGAGAGAGACCTTACGTGGAGCAGATAGTGCTTA
CTACCAGGTTGGGCAGACGGGCAAGGAGGGGACCGAGAATCAAGGCGTTGAGCCTCAAGA
TGAAGTAGATGGAGGAGATACACAAAAGAAGCAACTCATAAATCCTCATGTGGAACTTCA
ATTTTCAGATGCTAATGCCAAGTTTTACTGTCGGCTCTACTATGCGGGAGAGTTTCATAA
GATGCGTGAAGTGATTCTGGACAGCAGTGAAGAAGATTTCATTCGTTCCCTCTCCCACTC
ATCACCCTGGCAGGCCCGGGGAGGCAAATCAGGAGCTGCCTTCTATGCAACTGAGGATGA
TAGATTTATTTTGAAGCAAATGCCTCGTCTGGAAGTCCAGTCCTTCCTCGACTTTGCACC
ACATTACTTCAATTATATTACAAATGCTGTTCAACAAAAGAGGCCCACGGCGTTGGCCAA
AATTCTTGGAGTTTACAGAATTGGTTATAAGAACTCTCAGAACAACACTGAGAAGAAGTT
AGATCTCCTTGTCATGGAAAATCTTTTCTACGGGAGAAAGATGGCACAGGTTTTTGATTT
GAAGGGCTCTCTTAGGAATCGGAATGTAAAAACTGACACTGGAAAAGAGAGTTGTGATGT
GGTCCTGCTAGATGAAAATCTCCTAAAGATGGTTCGAGACAACCCTCTATATATTCGTTC
TCATTCCAAAGCTGTGCTGAGAACCTCGATCCATAGTGACTCCCATTTCCTTTCTAGCCA
CCTCATTATAGATTATTCTTTGCTGGTTGGGCGAGATGATACTAGCAATGAGCTAGTAGT
TGGAATTATAGATTATATTCGAACATTTACATGGGACAAAAAGCTTGAGATGGTTGTGAA
ATCAACAGGAATTTTAGGTGGACAAGGTAAAATGCCAACAGTGGTGTCTCCGGAGTTGTA
CAGGACTAGGTTTTGTGAGGCAATGGACAAGTATTTCCTAATGGTACCAGACCACTGGAC
AGGCTTGGGTCTGAATTGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT