KDM4E ORF clone pFN21AE3537

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3537

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Clone info.

Species

human

Clone Name

pFN21AE3537 Sequence (with vector) Restriction Map

Product ID

FHC30500

ORF Sequence

DNA sequence (1518 bp)
Protein sequence (506 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3537

Symbol

KDM4E

Alias : JMJD2E, KDM4DL, KDM5E

Description

lysine (K)-specific demethylase 4E

Original Clone ID

cp93537

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1518 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 506 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44713.1 4e-193 99.8 KDM4E CCDS8302.1 8e-118 67.4 KDM4D CCDS12138.1 1e-95 63.3 KDM4B CCDS55286.1 2e-89 64.8 KDM4C CCDS55285.1 2e-89 64.8 KDM4C The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003349 16 50 PF02375 Transcription factor jumonji IPR013129 176 292 PF02373 Transcription factor jumonji HMMSmart IPR003349 14 56 SM00545 Transcription factor jumonji IPR003347 143 309 SM00558 Transcription factor jumonji/aspartyl beta-hydroxylase ProfileScan IPR003349 15 57 PS51183 Transcription factor jumonji IPR003347 143 309 PS51184 Transcription factor jumonji/aspartyl beta-hydroxylase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001155102 506 616581 99.8 100.0 NP_060509 523 609766 67.4 98.6 NP_055830 1096 609765 63.3 81.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3537 Download

>KIEE3537  1518 bp
ATGAAGTCTGTGCACTCCAGTCCCCAGAACACGAGTCATACCATCATGACGTTTTACCCA
ACCATGGAAGAATTTGCAGATTTCAACACATATGTTGCTTACATGGAGTCCCAAGGCGCA
CATCAAGCTGGCCTTGCCAAGGTAATTCCACCCAAGGAATGGAAAGCCAGACAGATGTAT
GATGATATCGAAGACATCTTAATAGCCACTCCCCTCCAGCAGGTGACCTCTGGGCAGGGA
GGTGTGTTTACTCAATACCATAAAAAGAAGAAAGCCATGAGGGTGGGGCAGTATCGCCGC
TTGGCAAACAGTAAAAAATATCAGACTCCGCCACACCGGAATTTTGCAGATTTGGAGCAA
CGATACTGGAAGAGCCACCCCGGTAATCCACCAATTTATGGTGCTGATATCAGCGGCTCC
TTATTTGAAGAAAGCACTAAACAATGGAACCTAGGACACCTGGGAACAATTCTGGACCTG
TTGGAGCAGGAATGTGGGGTTGTCATCGAGGGTGTCAACACACCCTACCTGTACTTTGGC
ATGTGGAAGACCACGTTTGCCTGGCACACAGAGGACATGGACCTTTACAGCATCAACTAC
CTGCACTTTGGGGAGCCCAAAACTTGGTACGTGGTGCCCCCAGAACATGGTCAGCACCTG
GAACGCCTGGCCAGGGAGCTCTTCCCAGACATTTCTCGGGGCTGTGAGGCCTTCCTGCGG
CACAAAGTGGCCCTCATCTCGCCTACAGTTCTCAAGGAAAATGGGATTCCCTTCAATTGC
ATGACTCAGGAGGCTGGGGAGTTCATGGTGACCTTTCCCTATGGCTACCATGCTGGCTTC
AATCACGGCTTCAACTGCGCAGAAGCCATTAATTTTGCCACTCCACGATGGATTGATTAT
GGCAAAATGGCCTCTCAGTGTAGCTGTGGGGAGTCGACAGTGACCTTTTCCATGGACCCC
TTTGTGCGCATTGTGCAACCCGAGAGTTATGAGCTCTGGAAACACAGGCAAGACTTGGCC
ATTGTGGAACACACAGAGCCCAGGGTTGCAGAAAGCCAAGAGCTGAGCAACTGGAGAGAT
GATATAGTACTTAGAAGAGCTGCTCTGGGCCTGAGGCTTCTCCCAAACCTCACAGCCCAG
TGTCCCACACAGCCTGTGTCCTCAGGGCACTGTTACAACCCAAAAGGCTGTGGCACTGAT
GCTGTGCCTGGATCCGCATTCCAAAGCTCTGCATATCATACCCAGACCCAGTCACTTACC
CTGGGGATGTCAGCCAGGGTTCTTCTCCCTTCCACTGGAAGCTGGGGTTCTGGTCGTGGT
CGTGGTCGTGGTCAAGGTCAAGGTCGAGGTTGCAGTCGTGGTCGTGGTCATGGTTGTTGT
ACTCGAGAACTGGGGACTGAGGAGCCAACTGTTCAGCCTGCATCCAAGAGGCGCCTTTTA
ATGGGTACAAGGAGTAGAGCTCAAGGCCACAGGCCTCAGCTCCCGCTTGCCAATGATTTG
ATGACAAATCTGTCCCTT

ORF Protein

Cloned ORF protein sequence for pFN21AE3537 Download

>KIEE3537  506 aa
MKSVHSSPQNTSHTIMTFYPTMEEFADFNTYVAYMESQGAHQAGLAKVIPPKEWKARQMY
DDIEDILIATPLQQVTSGQGGVFTQYHKKKKAMRVGQYRRLANSKKYQTPPHRNFADLEQ
RYWKSHPGNPPIYGADISGSLFEESTKQWNLGHLGTILDLLEQECGVVIEGVNTPYLYFG
MWKTTFAWHTEDMDLYSINYLHFGEPKTWYVVPPEHGQHLERLARELFPDISRGCEAFLR
HKVALISPTVLKENGIPFNCMTQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDY
GKMASQCSCGESTVTFSMDPFVRIVQPESYELWKHRQDLAIVEHTEPRVAESQELSNWRD
DIVLRRAALGLRLLPNLTAQCPTQPVSSGHCYNPKGCGTDAVPGSAFQSSAYHTQTQSLT
LGMSARVLLPSTGSWGSGRGRGRGQGQGRGCSRGRGHGCCTRELGTEEPTVQPASKRRLL
MGTRSRAQGHRPQLPLANDLMTNLSL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3537 Download

>pFN21AE3537  6223 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGTCTGTGCACTCCAGTCCCCAGAACACGAG
TCATACCATCATGACGTTTTACCCAACCATGGAAGAATTTGCAGATTTCAACACATATGT
TGCTTACATGGAGTCCCAAGGCGCACATCAAGCTGGCCTTGCCAAGGTAATTCCACCCAA
GGAATGGAAAGCCAGACAGATGTATGATGATATCGAAGACATCTTAATAGCCACTCCCCT
CCAGCAGGTGACCTCTGGGCAGGGAGGTGTGTTTACTCAATACCATAAAAAGAAGAAAGC
CATGAGGGTGGGGCAGTATCGCCGCTTGGCAAACAGTAAAAAATATCAGACTCCGCCACA
CCGGAATTTTGCAGATTTGGAGCAACGATACTGGAAGAGCCACCCCGGTAATCCACCAAT
TTATGGTGCTGATATCAGCGGCTCCTTATTTGAAGAAAGCACTAAACAATGGAACCTAGG
ACACCTGGGAACAATTCTGGACCTGTTGGAGCAGGAATGTGGGGTTGTCATCGAGGGTGT
CAACACACCCTACCTGTACTTTGGCATGTGGAAGACCACGTTTGCCTGGCACACAGAGGA
CATGGACCTTTACAGCATCAACTACCTGCACTTTGGGGAGCCCAAAACTTGGTACGTGGT
GCCCCCAGAACATGGTCAGCACCTGGAACGCCTGGCCAGGGAGCTCTTCCCAGACATTTC
TCGGGGCTGTGAGGCCTTCCTGCGGCACAAAGTGGCCCTCATCTCGCCTACAGTTCTCAA
GGAAAATGGGATTCCCTTCAATTGCATGACTCAGGAGGCTGGGGAGTTCATGGTGACCTT
TCCCTATGGCTACCATGCTGGCTTCAATCACGGCTTCAACTGCGCAGAAGCCATTAATTT
TGCCACTCCACGATGGATTGATTATGGCAAAATGGCCTCTCAGTGTAGCTGTGGGGAGTC
GACAGTGACCTTTTCCATGGACCCCTTTGTGCGCATTGTGCAACCCGAGAGTTATGAGCT
CTGGAAACACAGGCAAGACTTGGCCATTGTGGAACACACAGAGCCCAGGGTTGCAGAAAG
CCAAGAGCTGAGCAACTGGAGAGATGATATAGTACTTAGAAGAGCTGCTCTGGGCCTGAG
GCTTCTCCCAAACCTCACAGCCCAGTGTCCCACACAGCCTGTGTCCTCAGGGCACTGTTA
CAACCCAAAAGGCTGTGGCACTGATGCTGTGCCTGGATCCGCATTCCAAAGCTCTGCATA
TCATACCCAGACCCAGTCACTTACCCTGGGGATGTCAGCCAGGGTTCTTCTCCCTTCCAC
TGGAAGCTGGGGTTCTGGTCGTGGTCGTGGTCGTGGTCAAGGTCAAGGTCGAGGTTGCAG
TCGTGGTCGTGGTCATGGTTGTTGTACTCGAGAACTGGGGACTGAGGAGCCAACTGTTCA
GCCTGCATCCAAGAGGCGCCTTTTAATGGGTACAAGGAGTAGAGCTCAAGGCCACAGGCC
TCAGCTCCCGCTTGCCAATGATTTGATGACAAATCTGTCCCTTGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT