AMFR ORF clone pFN21AE3264

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3264

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Clone info.

Species

human

Clone Name

pFN21AE3264 Sequence (with vector) Restriction Map

Product ID

FHC25706

ORF Sequence

DNA sequence (1929 bp)
Protein sequence (643 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3264

Symbol

AMFR

Alias : GP78, RNF45

Description

autocrine motility factor receptor, E3 ubiquitin protein ligase

Original Clone ID

cp93264

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1929 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 643 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10758.1 1e-197 100.0 AMFR CCDS8097.1 9.4e-15 28.8 SYVN1 CCDS31605.1 9.4e-15 28.8 SYVN1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 341 378 PF00097 Zinc finger IPR003892 458 497 PF02845 Ubiquitin system component Cue HMMSmart IPR001841 341 378 SM00184 Zinc finger IPR003892 456 497 SM00546 Ubiquitin system component Cue ProfileScan IPR001841 341 379 PS50089 Zinc finger IPR003892 456 498 PS51140 Ubiquitin system component Cue
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 16 TYTGLSGLALLGTIISAYRALSQ 38 SECONDARY 23 2 84 VWVLVNTACCVLMLVAKLIQCIV 106 PRIMARY 23 3 121 KFWNFIFYKFIFIFGVLNVQTVE 143 PRIMARY 23 4 145 VVMWCLWFAGLVFLHLMVQLCK 166 PRIMARY 22 5 183 HGRVLSLLVAMLLSCCGLAAVCS 205 PRIMARY 23 6 219 MAAESLLVTVRTAHVILRYVIHL 241 SECONDARY 23 7 279 MLLFGNIWLSMASLVIFMQLRYL 301 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001135 643 603243 100.0 100.0 XP_005255947 548 603243 100.0 85.2 NP_001310440 548 603243 100.0 85.2 NP_001310441 675 603243 95.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3264 Download

>KIEE3264  1929 bp
ATGCCGCTGCTCTTCCTCGAGCGCTTCCCCTGGCCCAGCCTCCGCACCTACACGGGCCTC
AGCGGCCTGGCCCTGCTGGGCACCATCATCAGCGCCTACCGCGCGCTCAGCCAGCCCGAG
GCCGGCCCCGGCGAGCCGGACCAGCTAACGGCCTCGCTGCAGCCTGAGCCGCCGGCGCCC
GCCCGGCCGAGCGCCGGGGGACCCCGGGCCCGCGATGTGGCCCAGTACCTGCTCTCAGAC
AGCCTCTTCGTGTGGGTTCTAGTAAATACCGCTTGCTGTGTTTTGATGTTGGTGGCTAAG
CTCATCCAGTGTATTGTGTTTGGCCCTCTTCGAGTGAGTGAGAGACAGCATCTCAAAGAC
AAATTTTGGAATTTTATTTTCTACAAGTTCATTTTCATCTTTGGTGTGCTGAATGTCCAG
ACAGTGGAAGAGGTGGTCATGTGGTGCCTCTGGTTTGCCGGACTTGTCTTTCTGCACCTG
ATGGTTCAGCTCTGCAAGGATCGATTTGAATATCTTTCCTTCTCGCCCACCACGCCGATG
AGCAGCCACGGTCGAGTCCTGTCCCTGTTGGTTGCCATGCTGCTTTCCTGCTGTGGACTG
GCGGCCGTCTGCTCCATCACCGGCTACACCCACGGAATGCACACCTTGGCTTTCATGGCT
GCAGAGTCTCTTCTTGTGACAGTGAGGACTGCTCATGTGATTTTACGATACGTAATTCAC
CTCTGGGACCTCAACCACGAAGGGACGTGGGAAGGAAAGGGGACGTATGTCTATTACACA
GACTTTGTCATGGAGCTCACTCTCCTGTCCCTGGACCTCATGCACCATATTCACATGTTG
TTATTTGGCAACATCTGGTTATCCATGGCCAGCCTGGTCATCTTTATGCAGCTGCGTTAC
CTGTTTCATGAGGTGCAACGTCGAATTCGTCGGCACAAGAACTATCTACGTGTGGTTGGA
AACATGGAGGCCAGGTTTGCAGTTGCAACTCCAGAGGAGCTGGCTGTCAACAATGACGAC
TGTGCCATCTGTTGGGACTCCATGCAGGCTGCGCGGAAACTGCCCTGTGGACATCTTTTC
CACAACTCCTGTCTTCGTTCCTGGCTAGAACAAGACACCTCCTGTCCAACATGCAGAATG
TCTCTTAATATTGCCGACAATAATCGTGTCAGGGAAGAACATCAAGGAGAGAACTTGGAT
GAGAATTTGGTTCCTGTAGCAGCAGCCGAAGGGAGACCTCGCTTAAACCAACACAATCAC
TTCTTCCATTTCGATGGGTCTCGGATTGCGAGCTGGCTGCCGAGTTTTTCGGTTGAAGTG
ATGCACACCACCAACATTCTTGGCATTACGCAGGCCAGCAACTCCCAGCTCAATGCAATG
GCTCATCAGATTCAAGAGATGTTTCCCCAGGTTCCATACCATCTGGTACTGCAGGACCTC
CAGCTGACACGCTCAGTTGAAATAACAACAGACAATATTTTAGAAGGACGGATTCAAGTA
CCTTTTCCTACACAGCGGTCAGATAGCATCAGACCTGCATTGAACAGTCCTGTGGAAAGG
CCAAGCAGTGACCAGGAAGAGGGAGAAACTTCTGCTCAGACCGAGCGTGTGCCACTGGAC
CTCAGTCCTCGCCTGGAGGAGACGCTGGACTTCGGCGAGGTGGAAGTGGAGCCCAGTGAG
GTGGAAGACTTCGAGGCTCGTGGGAGCCGCTTCTCCAAGTCTGCTGATGAGAGACAGCGC
ATGCTGGTGCAGCGTAAGGACGAACTCCTCCAGCAAGCTCGCAAACGTTTCTTGAACAAA
AGTTCTGAAGATGATGCGGCCTCAGAGAGCTTCCTCCCCTCGGAAGGTGCGTCCTCTGAC
CCCGTGACCCTGCGTCGAAGGATGCTGGCTGCCGCCGCGGAACGGAGGCTTCAGAAGCAG
CAGACCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE3264 Download

>KIEE3264  643 aa
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAP
ARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKD
KFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIH
LWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRY
LFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNH
FFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDL
QLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLD
LSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNK
SSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3264 Download

>pFN21AE3264  6634 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGCTGCTCTTCCTCGAGCGCTTCCCCTGGCC
CAGCCTCCGCACCTACACGGGCCTCAGCGGCCTGGCCCTGCTGGGCACCATCATCAGCGC
CTACCGCGCGCTCAGCCAGCCCGAGGCCGGCCCCGGCGAGCCGGACCAGCTAACGGCCTC
GCTGCAGCCTGAGCCGCCGGCGCCCGCCCGGCCGAGCGCCGGGGGACCCCGGGCCCGCGA
TGTGGCCCAGTACCTGCTCTCAGACAGCCTCTTCGTGTGGGTTCTAGTAAATACCGCTTG
CTGTGTTTTGATGTTGGTGGCTAAGCTCATCCAGTGTATTGTGTTTGGCCCTCTTCGAGT
GAGTGAGAGACAGCATCTCAAAGACAAATTTTGGAATTTTATTTTCTACAAGTTCATTTT
CATCTTTGGTGTGCTGAATGTCCAGACAGTGGAAGAGGTGGTCATGTGGTGCCTCTGGTT
TGCCGGACTTGTCTTTCTGCACCTGATGGTTCAGCTCTGCAAGGATCGATTTGAATATCT
TTCCTTCTCGCCCACCACGCCGATGAGCAGCCACGGTCGAGTCCTGTCCCTGTTGGTTGC
CATGCTGCTTTCCTGCTGTGGACTGGCGGCCGTCTGCTCCATCACCGGCTACACCCACGG
AATGCACACCTTGGCTTTCATGGCTGCAGAGTCTCTTCTTGTGACAGTGAGGACTGCTCA
TGTGATTTTACGATACGTAATTCACCTCTGGGACCTCAACCACGAAGGGACGTGGGAAGG
AAAGGGGACGTATGTCTATTACACAGACTTTGTCATGGAGCTCACTCTCCTGTCCCTGGA
CCTCATGCACCATATTCACATGTTGTTATTTGGCAACATCTGGTTATCCATGGCCAGCCT
GGTCATCTTTATGCAGCTGCGTTACCTGTTTCATGAGGTGCAACGTCGAATTCGTCGGCA
CAAGAACTATCTACGTGTGGTTGGAAACATGGAGGCCAGGTTTGCAGTTGCAACTCCAGA
GGAGCTGGCTGTCAACAATGACGACTGTGCCATCTGTTGGGACTCCATGCAGGCTGCGCG
GAAACTGCCCTGTGGACATCTTTTCCACAACTCCTGTCTTCGTTCCTGGCTAGAACAAGA
CACCTCCTGTCCAACATGCAGAATGTCTCTTAATATTGCCGACAATAATCGTGTCAGGGA
AGAACATCAAGGAGAGAACTTGGATGAGAATTTGGTTCCTGTAGCAGCAGCCGAAGGGAG
ACCTCGCTTAAACCAACACAATCACTTCTTCCATTTCGATGGGTCTCGGATTGCGAGCTG
GCTGCCGAGTTTTTCGGTTGAAGTGATGCACACCACCAACATTCTTGGCATTACGCAGGC
CAGCAACTCCCAGCTCAATGCAATGGCTCATCAGATTCAAGAGATGTTTCCCCAGGTTCC
ATACCATCTGGTACTGCAGGACCTCCAGCTGACACGCTCAGTTGAAATAACAACAGACAA
TATTTTAGAAGGACGGATTCAAGTACCTTTTCCTACACAGCGGTCAGATAGCATCAGACC
TGCATTGAACAGTCCTGTGGAAAGGCCAAGCAGTGACCAGGAAGAGGGAGAAACTTCTGC
TCAGACCGAGCGTGTGCCACTGGACCTCAGTCCTCGCCTGGAGGAGACGCTGGACTTCGG
CGAGGTGGAAGTGGAGCCCAGTGAGGTGGAAGACTTCGAGGCTCGTGGGAGCCGCTTCTC
CAAGTCTGCTGATGAGAGACAGCGCATGCTGGTGCAGCGTAAGGACGAACTCCTCCAGCA
AGCTCGCAAACGTTTCTTGAACAAAAGTTCTGAAGATGATGCGGCCTCAGAGAGCTTCCT
CCCCTCGGAAGGTGCGTCCTCTGACCCCGTGACCCTGCGTCGAAGGATGCTGGCTGCCGC
CGCGGAACGGAGGCTTCAGAAGCAGCAGACCTCCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT