ENAH ORF clone pFN21AE2520

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Halotag ORF Clone pFN21AE2520

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Clone info.

Species

human

Clone Name

pFN21AE2520 Sequence (with vector) Restriction Map

Product ID

FHC27775

ORF Sequence

DNA sequence (1773 bp)
Protein sequence (591 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2520

Symbol

ENAH

Alias : ENA, MENA, NDPP1

Description

enabled homolog (Drosophila), transcript variant 1

Original Clone ID

cp01648

Note

We obtained this clone using the E.coli strain DH10B at 25 deg C.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1773 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 591 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31041.1 1.5e-70 100.0 ENAH CCDS31040.1 1.1e-61 96.4 ENAH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 314 326 PR01217 NULL NULL 332 353 PR01217 NULL NULL 355 371 PR01217 NULL HMMPfam IPR000697 4 107 PF00568 EVH1 NULL 382 411 PF11643 NULL IPR014885 551 590 PF08776 VASP tetramerisation HMMSmart IPR000697 1 108 SM00461 EVH1 ProfileScan IPR000697 1 111 PS50229 EVH1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001008493 591 609061 100.0 100.0 XP_005273244 605 609061 100.0 99.8 XP_016857237 853 609061 100.0 99.8 XP_016857245 578 609061 100.0 97.8 XP_016857246 578 609061 100.0 97.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2520 Download

>KIEE2520  1773 bp
ATGAGTGAACAGAGTATCTGTCAGGCAAGAGCTGCTGTGATGGTTTATGATGATGCCAAT
AAGAAGTGGGTGCCAGCTGGTGGCTCAACTGGATTCAGCAGAGTTCATATCTATCACCAT
ACAGGCAACAACACATTCAGAGTGGTGGGCAGGAAGATTCAGGACCATCAGGTCGTGATA
AACTGTGCCATTCCTAAAGGGTTGAAGTACAATCAAGCTACACAGACCTTCCACCAGTGG
CGAGATGCTAGACAGGTGTATGGTCTCAACTTTGGCAGCAAAGAGGATGCCAATGTCTTC
GCAAGTGCCATGATGCATGCCTTAGAAGTGTTAAATTCACAGGAAACAGGGCCAACATTG
CCTAGACAAAACTCACAACTACCTGCTCAAGTTCAAAATGGCCCATCCCAAGAAGAATTG
GAAATTCAAAGAAGACAACTACAAGAACAGCAACGGCAAAAGGAGCTGGAGCGGGAAAGG
CTGGAGCGAGAAAGAATGGAAAGAGAAAGGTTGGAGAGAGAGAGGTTAGAAAGGGAAAGG
CTGGAGAGGGAGCGACTGGAACAAGAACAGCTGGAGAGAGAGAGACAAGAACGGGAACGG
CAGGAACGCCTGGAGCGGCAGGAACGCCTGGAGCGGCAGGAACGCCTGGAGCGGCAGGAA
CGCCTGGATCGGGAGAGGCAAGAAAGACAAGAACGAGAGAGGCTGGAGAGACTGGAACGG
GAGAGGCAAGAAAGGGAGCGACAAGAGCAGTTAGAAAGGGAACAGCTGGAATGGGAGAGA
GAGCGCAGAATATCAAGTGCTGCTGCCCCTGCCTCTGTTGAGACTCCTCTAAACTCTGTG
CTGGGAGACTCTTCTGCTTCTGAGCCAGGCTTGCAGGCAGCCTCTCAGCCGGCCGAGACT
CCATCCCAACAGGGCATTGTCTTGGGACCACTTGCACCTCCACCTCCTCCACCACTCCCA
CCAGGGCCTGCACAGGCTTCAGTAGCCCTCCCTCCTCCCCCAGGGCCCCCTCCACCTCCT
CCACTCCCATCCACCGGGCCTCCACCGCCCCCTCCTCCCCCTCCTCTCCCTAATCAAGTA
CCCCCTCCTCCTCCACCACCTCCTGCCCCACCCCTCCCTGCATCTGGATTCTTTTTGGCA
TCCATGTCAGAAGACAATCGCCCTTTAACTGGACTTGCAGCTGCAATTGCCGGAGCAAAA
CTTAGGAAAGTGTCACGGATGGAGGATACCTCTTTCCCAAGTGGAGGGAATGCTATTGGT
GTGAACTCCGCCTCATCTAAAACAGATACAGGCCGTGGAAATGGACCCCTTCCTTTAGGG
GGTAGTGGTTTAATGGAAGAAATGAGTGCCCTGCTGGCCAGGAGGAGAAGAATTGCTGAA
AAGGGATCAACAATAGAAACAGAACAAAAAGAGGACAAAGGTGAAGATTCAGAGCCTGTA
ACTTCTAAGGCCTCTTCAACAAGTACACCTGAACCAACAAGAAAACCTTGGGAAAGAACA
AATACAATGAATGGCAGCAAGTCACCTGTTATCTCCAGACGGGATTCTCCAAGGAAAAAT
CAGATTGTTTTTGACAACAGGTCCTATGATTCATTACACAGACCAAAATCCACACCCTTA
TCACAGCCCAGTGCCAATGGAGTCCAGACGGAAGGACTTGACTATGACAGGCTGAAGCAG
GACATTTTAGATGAAATGAGAAAAGAATTAACAAAGCTAAAAGAAGAGCTCATTGATGCA
ATCAGGCAGGAACTGAGCAAGTCAAATACTGCA

ORF Protein

Cloned ORF protein sequence for pFN21AE2520 Download

>KIEE2520  591 aa
MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVI
NCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQETGPTL
PRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERERLERERMERERLERERLERER
LERERLEQEQLERERQERERQERLERQERLERQERLERQERLDRERQERQERERLERLER
ERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAET
PSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQV
PPPPPPPPAPPLPASGFFLASMSEDNRPLTGLAAAIAGAKLRKVSRMEDTSFPSGGNAIG
VNSASSKTDTGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGSTIETEQKEDKGEDSEPV
TSKASSTSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPL
SQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSNTA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2520 Download

>pFN21AE2520  6478 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGAACAGAGTATCTGTCAGGCAAGAGCTGC
TGTGATGGTTTATGATGATGCCAATAAGAAGTGGGTGCCAGCTGGTGGCTCAACTGGATT
CAGCAGAGTTCATATCTATCACCATACAGGCAACAACACATTCAGAGTGGTGGGCAGGAA
GATTCAGGACCATCAGGTCGTGATAAACTGTGCCATTCCTAAAGGGTTGAAGTACAATCA
AGCTACACAGACCTTCCACCAGTGGCGAGATGCTAGACAGGTGTATGGTCTCAACTTTGG
CAGCAAAGAGGATGCCAATGTCTTCGCAAGTGCCATGATGCATGCCTTAGAAGTGTTAAA
TTCACAGGAAACAGGGCCAACATTGCCTAGACAAAACTCACAACTACCTGCTCAAGTTCA
AAATGGCCCATCCCAAGAAGAATTGGAAATTCAAAGAAGACAACTACAAGAACAGCAACG
GCAAAAGGAGCTGGAGCGGGAAAGGCTGGAGCGAGAAAGAATGGAAAGAGAAAGGTTGGA
GAGAGAGAGGTTAGAAAGGGAAAGGCTGGAGAGGGAGCGACTGGAACAAGAACAGCTGGA
GAGAGAGAGACAAGAACGGGAACGGCAGGAACGCCTGGAGCGGCAGGAACGCCTGGAGCG
GCAGGAACGCCTGGAGCGGCAGGAACGCCTGGATCGGGAGAGGCAAGAAAGACAAGAACG
AGAGAGGCTGGAGAGACTGGAACGGGAGAGGCAAGAAAGGGAGCGACAAGAGCAGTTAGA
AAGGGAACAGCTGGAATGGGAGAGAGAGCGCAGAATATCAAGTGCTGCTGCCCCTGCCTC
TGTTGAGACTCCTCTAAACTCTGTGCTGGGAGACTCTTCTGCTTCTGAGCCAGGCTTGCA
GGCAGCCTCTCAGCCGGCCGAGACTCCATCCCAACAGGGCATTGTCTTGGGACCACTTGC
ACCTCCACCTCCTCCACCACTCCCACCAGGGCCTGCACAGGCTTCAGTAGCCCTCCCTCC
TCCCCCAGGGCCCCCTCCACCTCCTCCACTCCCATCCACCGGGCCTCCACCGCCCCCTCC
TCCCCCTCCTCTCCCTAATCAAGTACCCCCTCCTCCTCCACCACCTCCTGCCCCACCCCT
CCCTGCATCTGGATTCTTTTTGGCATCCATGTCAGAAGACAATCGCCCTTTAACTGGACT
TGCAGCTGCAATTGCCGGAGCAAAACTTAGGAAAGTGTCACGGATGGAGGATACCTCTTT
CCCAAGTGGAGGGAATGCTATTGGTGTGAACTCCGCCTCATCTAAAACAGATACAGGCCG
TGGAAATGGACCCCTTCCTTTAGGGGGTAGTGGTTTAATGGAAGAAATGAGTGCCCTGCT
GGCCAGGAGGAGAAGAATTGCTGAAAAGGGATCAACAATAGAAACAGAACAAAAAGAGGA
CAAAGGTGAAGATTCAGAGCCTGTAACTTCTAAGGCCTCTTCAACAAGTACACCTGAACC
AACAAGAAAACCTTGGGAAAGAACAAATACAATGAATGGCAGCAAGTCACCTGTTATCTC
CAGACGGGATTCTCCAAGGAAAAATCAGATTGTTTTTGACAACAGGTCCTATGATTCATT
ACACAGACCAAAATCCACACCCTTATCACAGCCCAGTGCCAATGGAGTCCAGACGGAAGG
ACTTGACTATGACAGGCTGAAGCAGGACATTTTAGATGAAATGAGAAAAGAATTAACAAA
GCTAAAAGAAGAGCTCATTGATGCAATCAGGCAGGAACTGAGCAAGTCAAATACTGCAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT