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SpecieshumanProduct IDFHC15884Cloning SiteSgfI-PmeIGenBank AccessionGeneKIEE2486SymbolCACNA1I
Alias : Cav3.3, ca(v)3.3Descriptioncalcium channel, voltage-dependent, T type, alpha 1I subunit, transcript variant 1Original Clone IDcp01627NoteWe obtained this clone using the E.coli strain JM109.
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 6669 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 2223 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS46710.1 0 100.0 CACNA1I CCDS46711.1 0 98.3 CACNA1I CCDS58568.1 1.5e-161 53.0 CACNA1G CCDS45733.1 7.3e-160 53.2 CACNA1G CCDS58571.1 7.2e-159 53.6 CACNA1G
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR005445 59 75 PR01629 Voltage-dependent calcium channel IPR005445 101 117 PR01629 Voltage-dependent calcium channel IPR005445 430 444 PR01629 Voltage-dependent calcium channel IPR005445 658 671 PR01629 Voltage-dependent calcium channel IPR002077 802 828 PR00167 Voltage-dependent calcium channel IPR002077 1361 1373 PR00167 Voltage-dependent calcium channel IPR005445 1442 1457 PR01629 Voltage-dependent calcium channel HMMPfam IPR005821 118 397 PF00520 Ion transport IPR005821 674 860 PF00520 Ion transport IPR005821 1208 1430 PF00520 Ion transport IPR005821 1521 1728 PF00520 Ion transport
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 80 WFECVSMLVILLNCVTLGMYQPC 102 PRIMARY 23 2 120 DFIFIFFAMEMVLKMVALGIFG 141 PRIMARY 22 3 203 LDTLPMLGNVLLLCFFVFFIFGI 225 PRIMARY 23 4 336 GAINFDNIGYAWIVIFQVITLEG 358 SECONDARY 23 5 377 YFILLIIVGSFFMINLCLVVIAT 399 PRIMARY 23 6 640 KYFNRGIMMAILVNTVSMGIEH 661 SECONDARY 22 7 673 EICNVVFTSMFALEMILKLAAFG 695 SECONDARY 23 8 702 NPYNIFDSIIVIISIWEIVGQAD 724 SECONDARY 23 9 765 FCMLLMLFIFIFSILGMHIFGCK 787 PRIMARY 23 10 803 NFDSLLWAIVTVFQILTQEDWNV 825 SECONDARY 23 11 841 YFVALMTFGNYVLFNLLVAILVE 863 PRIMARY 23 12 1165 TIIAHKLFDYVVLAFIFLNCITI 1187 SECONDARY 23 13 1247 LVFVSIIDIVVSLASAGGAKILG 1269 PRIMARY 23 14 1306 IGNIVLICCAFFIIFGILGVQLF 1328 PRIMARY 23 15 1367 GQALMSLFVLASKDGWVNIMYNG 1389 SECONDARY 23 16 1409 LYFISFLLIVSFFVLNMFVGVVV 1431 PRIMARY 23 17 1487 HYLDIFITFIICLNVVTMSLEH 1508 PRIMARY 22 18 1522 CNYMFTTVFVLEAVLKLVAFGL 1543 SECONDARY 22 19 1550 RWNQLDLAIVLLSVMGITLEEIE 1572 PRIMARY 23 20 1576 ALPINPTIIRIMRVLRIARVLKL 1598 SECONDARY 23 21 1622 LGLLFMLLFFIYAALGVELFGKL 1644 PRIMARY 23 22 1705 VSPLYFVSFVLTAQFVLINVVVA 1727 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_066919 2223 608230 100.0 100.0 NP_001003406 2188 608230 98.3 100.0 NP_001243253 2314 604065,616795 53.0 95.4 NP_938200 2266 604065,616795 53.2 95.4 NP_001243263 2194 604065,616795 53.6 95.4 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AE2486 Download>KIEE2486 6669 bp ATGGCTGAGAGCGCCTCCCCGCCCTCCTCATCTGCAGCAGCCCCAGCCGCTGAGCCAGGA GTCACCACGGAGCAGCCCGGACCCCGGAGCCCCCCATCTTCCCCGCCAGGCCTGGAGGAG CCTCTGGATGGAGCTGATCCTCATGTCCCACACCCAGACCTGGCGCCTATTGCCTTCTTC TGCCTGCGACAGACCACCAGCCCCCGGAACTGGTGCATCAAGATGGTGTGCAACCCGTGG TTTGAATGTGTCAGCATGCTGGTGATCCTGCTGAACTGCGTGACACTTGGCATGTACCAG CCGTGCGACGACATGGACTGCCTGTCCGACCGCTGCAAGATCCTGCAGGTCTTTGATGAC TTCATCTTTATCTTCTTTGCCATGGAGATGGTGCTCAAGATGGTGGCCCTGGGGATTTTT GGCAAGAAGTGCTACCTCGGGGACACATGGAACCGCCTGGATTTCTTCATCGTCATGGCA GGGATGGTCGAGTACTCCCTGGACCTTCAGAACATCAACCTGTCAGCCATCCGCACCGTG CGCGTCCTGAGGCCCCTCAAAGCCATCAACCGCGTGCCCAGTATGCGGATCCTGGTGAAC CTGCTCCTGGACACACTGCCCATGCTGGGGAATGTCCTGCTGCTCTGCTTCTTTGTCTTC TTCATCTTTGGCATCATAGGTGTGCAGCTCTGGGCGGGCCTGCTGCGTAACCGCTGCTTC CTGGAGGAGAACTTCACCATACAAGGGGATGTGGCCTTGCCCCCATACTACCAGCCGGAG GAGGATGATGAGATGCCCTTCATCTGCTCCCTGTCGGGCGACAATGGGATAATGGGCTGC CATGAGATCCCCCCGCTCAAGGAGCAGGGCCGTGAGTGCTGCCTGTCCAAGGACGACGTC TACGACTTTGGGGCGGGGCGCCAGGACCTCAATGCCAGCGGCCTCTGTGTCAACTGGAAC CGTTACTACAATGTGTGCCGTACGGGCAGCGCCAACCCCCACAAGGGTGCCATCAACTTT GACAACATCGGTTATGCTTGGATTGTCATCTTCCAGGTGATCACTCTGGAAGGCTGGGTG GAGATCATGTACTACGTGATGGATGCTCACTCCTTCTACAACTTCATCTACTTCATCCTG CTTATCATAGTGGGCTCCTTCTTCATGATCAACCTGTGCCTCGTTGTCATAGCGACCCAG TTCTCGGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAGCGGCAGCGCTACCTG TCCTCCAGCACGGTGGCCAGCTACGCCGAGCCTGGCGACTGCTACGAGGAGATCTTCCAG TATGTCTGCCACATCCTGCGCAAGGCCAAGCGCCGCGCCCTGGGCCTCTACCAGGCCCTG CAGAGCCGGCGCCAGGCCCTGGGCCCGGAGGCCCCGGCCCCCGCCAAACCTGGGCCCCAC GCCAAGGAGCCCCGGCACTACCATGGGAAGACTAAGGGTCAGGGAGATGAAGGGAGACAT CTCGGAAGCCGGCATTGCCAGACTTTGCATGGGCCTGCCTCCCCTGGAAATGATCACTCG GGAAGAGAGCTGTGCCCGCAACATAGCCCCCTGGATGCGACGCCCCACACCCTGGTGCAG CCCATCCCCGCCACGCTGGCTTCCGATCCCGCCAGCTGCCCTTGCTGCCAGCATGAGGAC GGCCGGCGGCCCTCGGGCCTGGGCAGCACCGACTCGGGCCAGGAGGGCTCGGGCTCCGGG AGCTCCGCTGGTGGCGAGGACGAGGCGGATGGGGACGGGGCCCGGAGCAGCGAGGACGGA GCCTCCTCAGAACTGGGGAAGGAGGAGGAGGAGGAGGAGCAGGCGGATGGGGCGGTCTGG CTGTGCGGGGATGTGTGGCGGGAGACGCGAGCCAAGCTGCGCGGCATCGTGGACAGCAAG TACTTCAACCGGGGCATCATGATGGCCATCCTGGTCAACACCGTCAGCATGGGCATCGAG CACCACGAGCAGCCGGAGGAGCTGACCAACATCCTGGAGATCTGCAATGTGGTCTTCACC AGCATGTTTGCCCTGGAGATGATCCTGAAGCTGGCTGCATTTGGGCTCTTCGACTACCTG CGTAACCCCTACAACATCTTCGACAGCATCATTGTCATCATCAGCATCTGGGAGATCGTG GGGCAGGCGGACGGTGGGCTGTCGGTGCTGCGGACCTTCCGGCTGCTGCGCGTGCTGAAA CTGGTGCGCTTCATGCCTGCCCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCATGGAC AACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCATCCTTGGG ATGCATATTTTTGGCTGCAAGTTCAGCCTCCGCACGGACACTGGAGACACGGTGCCCGAC AGGAAGAACTTCGACTCCCTGCTGTGGGCCATCGTCACTGTGTTCCAGATCCTCACCCAG GAGGACTGGAACGTCGTTCTCTACAATGGCATGGCCTCCACTTCTCCCTGGGCCTCCCTC TACTTTGTCGCCCTCATGACCTTCGGCAACTATGTGCTCTTCAACCTGCTGGTGGCCATC CTGGTGGAGGGCTTCCAGGCGGAGGGTGACGCCAATCGCTCCTACTCGGACGAGGACCAG AGCTCATCCAACATAGAAGAGTTTGATAAGCTCCAGGAAGGCCTGGACAGCAGCGGAGAT CCCAAGCTCTGCCCAATCCCCATGACCCCCAATGGGCACCTGGACCCCAGTCTCCCACTG GGTGGGCACCTAGGTCCTGCTGGGGCTGCGGGACCTGCCCCCCGACTCTCACTGCAGCCG GACCCCATGCTGGTGGCCCTGGGCTCCCGAAAGAGCAGCGTCATGTCTCTAGGGAGGATG AGCTATGACCAGCGCTCCCTGTCCAGCTCCCGGAGCTCCTACTACGGGCCATGGGGCCGC AGCGCGGCCTGGGCCAGCCGTCGCTCCAGCTGGAACAGCCTCAAGCACAAGCCGCCGTCG GCGGAGCATGAGTCCCTGCTCTCTGCGGAGCGCGGCGGCGGCGCCCGGGTCTGCGAGGTT GCCGCGGACGAGGGGCCGCCGCGGGCCGCACCCCTGCACACCCCACACGCCCACCACGTT CATCACGGGCCCCATCTGGCGCACCGCCACCGCCACCACCGCCGGACGCTGTCCCTCGAC AACAGGGACTCGGTGGACCTGGCCGAGCTGGTGCCCGCGGTGGGCGCCCACCCCCGGGCC GCCTGGAGGGCGGCAGGCCCGGCCCCCGGGCATGAGGACTGCAATGGCAGGATGCCCAGC ATCGCCAAAGACGTCTTCACCAAGATGGGCGACCGCGGGGATCGCGGGGAGGATGAGGAG GAAATCGACTACACCCTGTGCTTCCGCGTCCGCAAGATGATCGACGTCTATAAGCCCGAC TGGTGCGAGGTCCGCGAAGACTGGTCTGTCTACCTCTTCTCTCCCGAGAACAGGTTCCGG GTCCTGTGTCAGACCATTATTGCCCACAAACTCTTCGACTACGTCGTCCTGGCCTTCATC TTTCTCAACTGCATCACCATCGCCCTGGAGCGGCCTCAGATCGAGGCCGGCAGCACCGAA CGCATCTTTCTCACCGTGTCCAACTACATCTTCACGGCCATCTTCGTGGGCGAGATGACA TTGAAGGTAGTCTCGCTGGGCCTGTACTTCGGCGAGCAGGCGTACCTACGCAGCAGCTGG AACGTGCTGGATGGCTTTCTTGTCTTCGTGTCCATCATCGACATCGTGGTGTCCCTGGCC TCAGCCGGGGGAGCCAAGATCTTGGGGGTCCTCCGAGTCTTGCGGCTCCTGCGCACCCTA CGCCCCCTGCGTGTCATCAGCCGGGCGCCGGGCCTGAAGCTGGTGGTGGAGACACTCATC TCCTCCCTCAAGCCCATCGGCAACATCGTGCTCATCTGCTGTGCCTTCTTCATCATCTTT GGCATCCTGGGAGTGCAGCTCTTCAAGGGCAAGTTCTACCACTGTCTGGGCGTGGACACC CGCAACATCACCAACCGCTCGGACTGCATGGCCGCCAACTACCGCTGGGTCCATCACAAA TACAACTTCGACAACCTGGGCCAGGCTCTGATGTCCCTCTTTGTCCTGGCATCCAAGGAT GGTTGGGTGAACATCATGTACAATGGACTGGATGCTGTTGCTGTGGACCAGCAGCCTGTG ACCAACCACAACCCCTGGATGCTGCTGTACTTCATCTCCTTCCTGCTCATCGTCAGCTTC TTTGTGCTCAACATGTTTGTGGGTGTCGTGGTGGAGAACTTCCACAAGTGCCGGCAGCAC CAGGAGGCTGAAGAGGCACGGCGGCGTGAGGAGAAGCGGCTGCGGCGCCTGGAGAAGAAG CGCCGGAAGGCCCAGCGGCTGCCCTACTATGCCACCTATTGTCACACCCGGCTGCTCATC CACTCCATGTGCACCAGCCACTACCTGGACATCTTCATCACCTTCATCATCTGCCTCAAC GTGGTCACCATGTCCCTGGAGCACTACAATCAGCCCACGTCCCTGGAGACAGCCCTCAAG TACTGCAACTATATGTTCACCACTGTCTTTGTGCTGGAGGCTGTGCTGAAGCTGGTGGCA TTTGGTCTGAGGCGCTTCTTCAAGGACCGATGGAACCAGCTGGACCTGGCCATTGTGCTA CTGTCAGTCATGGGCATCACCCTGGAGGAGATCGAGATCAATGCGGCCCTGCCCATCAAT CCCACCATCATCCGCATCATGAGGGTTCTGCGCATTGCCCGAGTGCTGAAGCTGTTGAAG ATGGCCACAGGAATGCGGGCCCTGCTGGACACGGTGGTGCAAGCTTTGCCCCAGGTGGGC AACCTGGGCCTCCTCTTCATGCTGCTCTTCTTCATCTATGCTGCTCTCGGGGTGGAGCTC TTTGGGAAGCTGGTCTGCAACGACGAGAACCCGTGCGAGGGCATGAGCCGGCATGCCACC TTCGAGAACTTCGGCATGGCCTTCCTCACACTCTTCCAGGTCTCCACGGGTGACAACTGG AACGGGATCATGAAGGACACGCTGCGGGACTGCACCCACGACGAGCGCAGCTGCCTGAGC AGCCTGCAGTTTGTGTCGCCGCTGTACTTCGTGAGCTTCGTGCTCACCGCGCAGTTCGTG CTCATCAACGTGGTGGTGGCTGTGCTCATGAAGCACCTGGACGACAGCAACAAGGAGGCG CAGGAGGACGCCGAGATGGATGCCGAGCTCGAGCTGGAGATGGCCCATGGCCTGGGCCCT GGCCCGAGGCTGCCTACCGGCTCCCCGGGCGCCCCTGGCCGAGGGCCGGGAGGGGCGGGC GGCGGGGGCGACACCGAGGGCGGCTTGTGCCGGCGCTGCTACTCGCCTGCCCAGGAGAAC CTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCTGACCATC ATCGACAACCTGTCGGGCTCCATCTTCCACCACTACTCCTCGCCTGCCGGCTGCAAGAAG TGTCACCACGACAAGCAAGAGGTGCAGCTGGCTGAGACGGAGGCCTTCTCCCTGAACTCA GACAGGTCCTCGTCCATCCTGCTGGGTGACGACCTGAGTCTCGAGGACCCCACAGCCTGC CCACCTGGCCGCAAAGACAGCAAGGGTGAGCTGGACCCACCTGAGCCCATGCGTGTGGGA GACCTGGGCGAATGCTTCTTCCCCTTGTCCTCTACGGCCGTCTCGCCGGATCCAGAGAAC TTCCTGTGTGAGATGGAGGAGATCCCATTCAACCCTGTCCGGTCCTGGCTGAAACATGAC AGCAGTCAAGCACCCCCAAGTCCCTTCTCCCCGGATGCCTCCAGCCCTCTCCTGCCCATG CCAGCCGAGTTCTTCCACCCTGCAGTGTCTGCCAGCCAGAAAGGCCCAGAAAAGGGCACT GGCACTGGAACCCTCCCCAAGATTGCGCTGCAGGGCTCCTGGGCATCTCTGCGGTCACCA AGGGTCAACTGTACCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGACGCCAGC CCCAGCAGCTCCGCGGGCAGCCTGCAGACCACGCTCGAGGACAGCCTGACCCTGAGCGAC AGCCCCCGGCGTGCCCTGGGGCCGCCCGCGCCTGCTCCAGGACCCCGGGCCGGCCTGTCC CCCGCCGCTCGCCGCCGCCTGAGCCTGCGCGGCCGGGGCCTCTTCAGCCTGCGGGGGCTG CGGGCGCATCAGCGCAGCCACAGCAGCGGGGGCTCCACCAGCCCGGGCTGCACCCACCAC GACTCCATGGACCCCTCGGACGAGGAGGGCCGCGGTGGCGCGGGCGGCGGGGGCGCGGGC AGCGAGCACTCGGAGACCCTCAGCAGCCTCTCGCTCACCTCCCTCTTCTGCCCGCCGCCC CCGCCGCCAGCCCCCGGCCTCACGCCCGCCAGGAAGTTCAGCAGCACCAGCAGCCTGGCC GCCCCCGGCCGCCCCCACGCCGCCGCCCTGGCCCACGGCCTGGCCCGGAGCCCCTCGTGG GCCGCGGACCGCAGCAAGGACCCCCCCGGCCGGGCACCGCTGCCCATGGGCCTGGGCCCC TTGGCGCCCCCGCCGCAACCGCTCCCCGGAGAGCTGGAGCCGGGAGACGCCGCCAGCAAG AGGAAGAGA
Cloned ORF protein sequence for pFN21AE2486 Download>KIEE2486 2223 aa MAESASPPSSSAAAPAAEPGVTTEQPGPRSPPSSPPGLEEPLDGADPHVPHPDLAPIAFF CLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMDCLSDRCKILQVFDD FIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTV RVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCF LEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDV YDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWV EIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYL SSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQSRRQALGPEAPAPAKPGPH AKEPRHYHGKTKGQGDEGRHLGSRHCQTLHGPASPGNDHSGRELCPQHSPLDATPHTLVQ PIPATLASDPASCPCCQHEDGRRPSGLGSTDSGQEGSGSGSSAGGEDEADGDGARSSEDG ASSELGKEEEEEEQADGAVWLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIE HHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIV GQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILG MHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASL YFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNIEEFDKLQEGLDSSGD PKLCPIPMTPNGHLDPSLPLGGHLGPAGAAGPAPRLSLQPDPMLVALGSRKSSVMSLGRM SYDQRSLSSSRSSYYGPWGRSAAWASRRSSWNSLKHKPPSAEHESLLSAERGGGARVCEV AADEGPPRAAPLHTPHAHHVHHGPHLAHRHRHHRRTLSLDNRDSVDLAELVPAVGAHPRA AWRAAGPAPGHEDCNGRMPSIAKDVFTKMGDRGDRGEDEEEIDYTLCFRVRKMIDVYKPD WCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTE RIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLA SAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIF GILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKD GWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQH QEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCHTRLLIHSMCTSHYLDIFITFIICLN VVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVL LSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVG NLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNW NGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEA QEDAEMDAELELEMAHGLGPGPRLPTGSPGAPGRGPGGAGGGGDTEGGLCRRCYSPAQEN LWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCKKCHHDKQEVQLAETEAFSLNS DRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVGDLGECFFPLSSTAVSPDPEN FLCEMEEIPFNPVRSWLKHDSSQAPPSPFSPDASSPLLPMPAEFFHPAVSASQKGPEKGT GTGTLPKIALQGSWASLRSPRVNCTLLRQATGSDTSLDASPSSSAGSLQTTLEDSLTLSD SPRRALGPPAPAPGPRAGLSPAARRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTHH DSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCPPPPPPAPGLTPARKFSSTSSLA APGRPHAAALAHGLARSPSWAADRSKDPPGRAPLPMGLGPLAPPPQPLPGELEPGDAASK RKR
Nucleotide Sequence (with vector) for pFN21AE2486 Download>pFN21AE2486 11374 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGGCTGAGAGCGCCTCCCCGCCCTCCTCATCTGC AGCAGCCCCAGCCGCTGAGCCAGGAGTCACCACGGAGCAGCCCGGACCCCGGAGCCCCCC ATCTTCCCCGCCAGGCCTGGAGGAGCCTCTGGATGGAGCTGATCCTCATGTCCCACACCC AGACCTGGCGCCTATTGCCTTCTTCTGCCTGCGACAGACCACCAGCCCCCGGAACTGGTG CATCAAGATGGTGTGCAACCCGTGGTTTGAATGTGTCAGCATGCTGGTGATCCTGCTGAA CTGCGTGACACTTGGCATGTACCAGCCGTGCGACGACATGGACTGCCTGTCCGACCGCTG CAAGATCCTGCAGGTCTTTGATGACTTCATCTTTATCTTCTTTGCCATGGAGATGGTGCT CAAGATGGTGGCCCTGGGGATTTTTGGCAAGAAGTGCTACCTCGGGGACACATGGAACCG CCTGGATTTCTTCATCGTCATGGCAGGGATGGTCGAGTACTCCCTGGACCTTCAGAACAT CAACCTGTCAGCCATCCGCACCGTGCGCGTCCTGAGGCCCCTCAAAGCCATCAACCGCGT GCCCAGTATGCGGATCCTGGTGAACCTGCTCCTGGACACACTGCCCATGCTGGGGAATGT CCTGCTGCTCTGCTTCTTTGTCTTCTTCATCTTTGGCATCATAGGTGTGCAGCTCTGGGC GGGCCTGCTGCGTAACCGCTGCTTCCTGGAGGAGAACTTCACCATACAAGGGGATGTGGC CTTGCCCCCATACTACCAGCCGGAGGAGGATGATGAGATGCCCTTCATCTGCTCCCTGTC GGGCGACAATGGGATAATGGGCTGCCATGAGATCCCCCCGCTCAAGGAGCAGGGCCGTGA GTGCTGCCTGTCCAAGGACGACGTCTACGACTTTGGGGCGGGGCGCCAGGACCTCAATGC CAGCGGCCTCTGTGTCAACTGGAACCGTTACTACAATGTGTGCCGTACGGGCAGCGCCAA CCCCCACAAGGGTGCCATCAACTTTGACAACATCGGTTATGCTTGGATTGTCATCTTCCA GGTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTACGTGATGGATGCTCACTCCTT CTACAACTTCATCTACTTCATCCTGCTTATCATAGTGGGCTCCTTCTTCATGATCAACCT GTGCCTCGTTGTCATAGCGACCCAGTTCTCGGAGACCAAGCAACGGGAGCACCGGCTGAT GCTGGAGCAGCGGCAGCGCTACCTGTCCTCCAGCACGGTGGCCAGCTACGCCGAGCCTGG CGACTGCTACGAGGAGATCTTCCAGTATGTCTGCCACATCCTGCGCAAGGCCAAGCGCCG CGCCCTGGGCCTCTACCAGGCCCTGCAGAGCCGGCGCCAGGCCCTGGGCCCGGAGGCCCC GGCCCCCGCCAAACCTGGGCCCCACGCCAAGGAGCCCCGGCACTACCATGGGAAGACTAA GGGTCAGGGAGATGAAGGGAGACATCTCGGAAGCCGGCATTGCCAGACTTTGCATGGGCC TGCCTCCCCTGGAAATGATCACTCGGGAAGAGAGCTGTGCCCGCAACATAGCCCCCTGGA TGCGACGCCCCACACCCTGGTGCAGCCCATCCCCGCCACGCTGGCTTCCGATCCCGCCAG CTGCCCTTGCTGCCAGCATGAGGACGGCCGGCGGCCCTCGGGCCTGGGCAGCACCGACTC GGGCCAGGAGGGCTCGGGCTCCGGGAGCTCCGCTGGTGGCGAGGACGAGGCGGATGGGGA CGGGGCCCGGAGCAGCGAGGACGGAGCCTCCTCAGAACTGGGGAAGGAGGAGGAGGAGGA GGAGCAGGCGGATGGGGCGGTCTGGCTGTGCGGGGATGTGTGGCGGGAGACGCGAGCCAA GCTGCGCGGCATCGTGGACAGCAAGTACTTCAACCGGGGCATCATGATGGCCATCCTGGT CAACACCGTCAGCATGGGCATCGAGCACCACGAGCAGCCGGAGGAGCTGACCAACATCCT GGAGATCTGCAATGTGGTCTTCACCAGCATGTTTGCCCTGGAGATGATCCTGAAGCTGGC TGCATTTGGGCTCTTCGACTACCTGCGTAACCCCTACAACATCTTCGACAGCATCATTGT CATCATCAGCATCTGGGAGATCGTGGGGCAGGCGGACGGTGGGCTGTCGGTGCTGCGGAC CTTCCGGCTGCTGCGCGTGCTGAAACTGGTGCGCTTCATGCCTGCCCTGCGGCGCCAGCT CGTGGTGCTCATGAAGACCATGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTT CATCTTCATCTTCAGCATCCTTGGGATGCATATTTTTGGCTGCAAGTTCAGCCTCCGCAC GGACACTGGAGACACGGTGCCCGACAGGAAGAACTTCGACTCCCTGCTGTGGGCCATCGT CACTGTGTTCCAGATCCTCACCCAGGAGGACTGGAACGTCGTTCTCTACAATGGCATGGC CTCCACTTCTCCCTGGGCCTCCCTCTACTTTGTCGCCCTCATGACCTTCGGCAACTATGT GCTCTTCAACCTGCTGGTGGCCATCCTGGTGGAGGGCTTCCAGGCGGAGGGTGACGCCAA TCGCTCCTACTCGGACGAGGACCAGAGCTCATCCAACATAGAAGAGTTTGATAAGCTCCA GGAAGGCCTGGACAGCAGCGGAGATCCCAAGCTCTGCCCAATCCCCATGACCCCCAATGG GCACCTGGACCCCAGTCTCCCACTGGGTGGGCACCTAGGTCCTGCTGGGGCTGCGGGACC TGCCCCCCGACTCTCACTGCAGCCGGACCCCATGCTGGTGGCCCTGGGCTCCCGAAAGAG CAGCGTCATGTCTCTAGGGAGGATGAGCTATGACCAGCGCTCCCTGTCCAGCTCCCGGAG CTCCTACTACGGGCCATGGGGCCGCAGCGCGGCCTGGGCCAGCCGTCGCTCCAGCTGGAA CAGCCTCAAGCACAAGCCGCCGTCGGCGGAGCATGAGTCCCTGCTCTCTGCGGAGCGCGG CGGCGGCGCCCGGGTCTGCGAGGTTGCCGCGGACGAGGGGCCGCCGCGGGCCGCACCCCT GCACACCCCACACGCCCACCACGTTCATCACGGGCCCCATCTGGCGCACCGCCACCGCCA CCACCGCCGGACGCTGTCCCTCGACAACAGGGACTCGGTGGACCTGGCCGAGCTGGTGCC CGCGGTGGGCGCCCACCCCCGGGCCGCCTGGAGGGCGGCAGGCCCGGCCCCCGGGCATGA GGACTGCAATGGCAGGATGCCCAGCATCGCCAAAGACGTCTTCACCAAGATGGGCGACCG CGGGGATCGCGGGGAGGATGAGGAGGAAATCGACTACACCCTGTGCTTCCGCGTCCGCAA GATGATCGACGTCTATAAGCCCGACTGGTGCGAGGTCCGCGAAGACTGGTCTGTCTACCT CTTCTCTCCCGAGAACAGGTTCCGGGTCCTGTGTCAGACCATTATTGCCCACAAACTCTT CGACTACGTCGTCCTGGCCTTCATCTTTCTCAACTGCATCACCATCGCCCTGGAGCGGCC TCAGATCGAGGCCGGCAGCACCGAACGCATCTTTCTCACCGTGTCCAACTACATCTTCAC GGCCATCTTCGTGGGCGAGATGACATTGAAGGTAGTCTCGCTGGGCCTGTACTTCGGCGA GCAGGCGTACCTACGCAGCAGCTGGAACGTGCTGGATGGCTTTCTTGTCTTCGTGTCCAT CATCGACATCGTGGTGTCCCTGGCCTCAGCCGGGGGAGCCAAGATCTTGGGGGTCCTCCG AGTCTTGCGGCTCCTGCGCACCCTACGCCCCCTGCGTGTCATCAGCCGGGCGCCGGGCCT GAAGCTGGTGGTGGAGACACTCATCTCCTCCCTCAAGCCCATCGGCAACATCGTGCTCAT CTGCTGTGCCTTCTTCATCATCTTTGGCATCCTGGGAGTGCAGCTCTTCAAGGGCAAGTT CTACCACTGTCTGGGCGTGGACACCCGCAACATCACCAACCGCTCGGACTGCATGGCCGC CAACTACCGCTGGGTCCATCACAAATACAACTTCGACAACCTGGGCCAGGCTCTGATGTC CCTCTTTGTCCTGGCATCCAAGGATGGTTGGGTGAACATCATGTACAATGGACTGGATGC TGTTGCTGTGGACCAGCAGCCTGTGACCAACCACAACCCCTGGATGCTGCTGTACTTCAT CTCCTTCCTGCTCATCGTCAGCTTCTTTGTGCTCAACATGTTTGTGGGTGTCGTGGTGGA GAACTTCCACAAGTGCCGGCAGCACCAGGAGGCTGAAGAGGCACGGCGGCGTGAGGAGAA GCGGCTGCGGCGCCTGGAGAAGAAGCGCCGGAAGGCCCAGCGGCTGCCCTACTATGCCAC CTATTGTCACACCCGGCTGCTCATCCACTCCATGTGCACCAGCCACTACCTGGACATCTT CATCACCTTCATCATCTGCCTCAACGTGGTCACCATGTCCCTGGAGCACTACAATCAGCC CACGTCCCTGGAGACAGCCCTCAAGTACTGCAACTATATGTTCACCACTGTCTTTGTGCT GGAGGCTGTGCTGAAGCTGGTGGCATTTGGTCTGAGGCGCTTCTTCAAGGACCGATGGAA CCAGCTGGACCTGGCCATTGTGCTACTGTCAGTCATGGGCATCACCCTGGAGGAGATCGA GATCAATGCGGCCCTGCCCATCAATCCCACCATCATCCGCATCATGAGGGTTCTGCGCAT TGCCCGAGTGCTGAAGCTGTTGAAGATGGCCACAGGAATGCGGGCCCTGCTGGACACGGT GGTGCAAGCTTTGCCCCAGGTGGGCAACCTGGGCCTCCTCTTCATGCTGCTCTTCTTCAT CTATGCTGCTCTCGGGGTGGAGCTCTTTGGGAAGCTGGTCTGCAACGACGAGAACCCGTG CGAGGGCATGAGCCGGCATGCCACCTTCGAGAACTTCGGCATGGCCTTCCTCACACTCTT CCAGGTCTCCACGGGTGACAACTGGAACGGGATCATGAAGGACACGCTGCGGGACTGCAC CCACGACGAGCGCAGCTGCCTGAGCAGCCTGCAGTTTGTGTCGCCGCTGTACTTCGTGAG CTTCGTGCTCACCGCGCAGTTCGTGCTCATCAACGTGGTGGTGGCTGTGCTCATGAAGCA CCTGGACGACAGCAACAAGGAGGCGCAGGAGGACGCCGAGATGGATGCCGAGCTCGAGCT GGAGATGGCCCATGGCCTGGGCCCTGGCCCGAGGCTGCCTACCGGCTCCCCGGGCGCCCC TGGCCGAGGGCCGGGAGGGGCGGGCGGCGGGGGCGACACCGAGGGCGGCTTGTGCCGGCG CTGCTACTCGCCTGCCCAGGAGAACCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGA CTCCTTGGAGGGGGAGCTGACCATCATCGACAACCTGTCGGGCTCCATCTTCCACCACTA CTCCTCGCCTGCCGGCTGCAAGAAGTGTCACCACGACAAGCAAGAGGTGCAGCTGGCTGA GACGGAGGCCTTCTCCCTGAACTCAGACAGGTCCTCGTCCATCCTGCTGGGTGACGACCT GAGTCTCGAGGACCCCACAGCCTGCCCACCTGGCCGCAAAGACAGCAAGGGTGAGCTGGA CCCACCTGAGCCCATGCGTGTGGGAGACCTGGGCGAATGCTTCTTCCCCTTGTCCTCTAC GGCCGTCTCGCCGGATCCAGAGAACTTCCTGTGTGAGATGGAGGAGATCCCATTCAACCC TGTCCGGTCCTGGCTGAAACATGACAGCAGTCAAGCACCCCCAAGTCCCTTCTCCCCGGA TGCCTCCAGCCCTCTCCTGCCCATGCCAGCCGAGTTCTTCCACCCTGCAGTGTCTGCCAG CCAGAAAGGCCCAGAAAAGGGCACTGGCACTGGAACCCTCCCCAAGATTGCGCTGCAGGG CTCCTGGGCATCTCTGCGGTCACCAAGGGTCAACTGTACCCTCCTCCGGCAGGCCACCGG GAGCGACACGTCGCTGGACGCCAGCCCCAGCAGCTCCGCGGGCAGCCTGCAGACCACGCT CGAGGACAGCCTGACCCTGAGCGACAGCCCCCGGCGTGCCCTGGGGCCGCCCGCGCCTGC TCCAGGACCCCGGGCCGGCCTGTCCCCCGCCGCTCGCCGCCGCCTGAGCCTGCGCGGCCG GGGCCTCTTCAGCCTGCGGGGGCTGCGGGCGCATCAGCGCAGCCACAGCAGCGGGGGCTC CACCAGCCCGGGCTGCACCCACCACGACTCCATGGACCCCTCGGACGAGGAGGGCCGCGG TGGCGCGGGCGGCGGGGGCGCGGGCAGCGAGCACTCGGAGACCCTCAGCAGCCTCTCGCT CACCTCCCTCTTCTGCCCGCCGCCCCCGCCGCCAGCCCCCGGCCTCACGCCCGCCAGGAA GTTCAGCAGCACCAGCAGCCTGGCCGCCCCCGGCCGCCCCCACGCCGCCGCCCTGGCCCA CGGCCTGGCCCGGAGCCCCTCGTGGGCCGCGGACCGCAGCAAGGACCCCCCCGGCCGGGC ACCGCTGCCCATGGGCCTGGGCCCCTTGGCGCCCCCGCCGCAACCGCTCCCCGGAGAGCT GGAGCCGGGAGACGCCGCCAGCAAGAGGAAGAGAGTTTAAACGAATTCGGGCTCGGTACC CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT
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