WDR82 ORF clone pFN21AE1227

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Halotag ORF Clone pFN21AE1227

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Clone info.

Species

human

Clone Name

pFN21AE1227 Sequence (with vector) Restriction Map

Product ID

FHC21568

ORF Sequence

DNA sequence (939 bp)
Protein sequence (313 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590669]

Gene

KIEE1227

Symbol

WDR82

Alias : MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WDR82A

Description

WD repeat domain 82

Original Clone ID

cs03127

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 939 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 313 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2851.2 1.2e-102 100.0 WDR82 CCDS6981.1 3.4e-15 27.1 WDR5 CCDS3012.1 6.1e-15 26.0 WDR5B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 36 50 PR00320 G-protein beta WD-40 repeat IPR020472 122 136 PR00320 G-protein beta WD-40 repeat IPR020472 254 268 PR00320 G-protein beta WD-40 repeat HMMPfam IPR019781 15 49 PF00400 WD40 repeat IPR019781 99 135 PF00400 WD40 repeat IPR019781 246 267 PF00400 WD40 repeat IPR019781 280 302 PF00400 WD40 repeat HMMSmart IPR001680 10 49 SM00320 WD40 repeat IPR001680 96 135 SM00320 WD40 repeat IPR001680 138 175 SM00320 WD40 repeat IPR001680 180 222 SM00320 WD40 repeat IPR001680 225 267 SM00320 WD40 repeat IPR001680 270 308 SM00320 WD40 repeat ProfileScan IPR019782 17 58 PS50082 WD40 repeat 2 IPR017986 17 313 PS50294 WD40-repeat-containing domain IPR019782 103 138 PS50082 WD40 repeat 2 IPR019782 235 276 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_079498 313 611059 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1227 Download

>KIEE1227  939 bp
ATGAAGCTGACCGACAGCGTGTTGCGGAGCTTCCGCGTCGCTAAGGTGTTCCGCGAAAAC
TCGGACAAGATTAACTGCTTCGATTTCAGCCCCAACGGCGAGACGGTCATCTCGAGTAGC
GACGACGACTCCATCGTGCTCTATGACTGCCAGGAGGGCAAACCAAAGAGAACCCTGTAC
AGTAAGAAATATGGTGTGGACCTCATCAGATACACTCATGCAGCAAACACAGTTGTTTAC
AGCTCTAACAAAATAGACGATACTATTCGTTACTTGTCCTTGCATGACAACAAATACATC
AGATACTTTCCTGGACATAGCAAAAGGGTGGTGGCCTTGTCCATGTCACCTGTGGATGAC
ACTTTCATTTCTGGGTCTCTTGATAAGACCATTCGACTCTGGGATCTCCGGTCTCCTAAC
TGCCAGGGCCTCATGCATCTGCAGGGGAAGCCAGTTTGTTCTTTTGATCCAGAAGGGTTA
ATTTTCGCTGCAGGTGTCAACTCTGAAATGGTCAAGCTTTATGACCTTCGTTCTTTTGAT
AAGGGGCCATTTGCTACCTTTAAGATGCAGTATGATCGAACTTGTGAGTGGACAGGACTT
AAATTCAGCAATGATGGCAAGCTCATCCTCATTTCCACCAACGGCAGCTTCATTCGTCTG
ATTGATGCATTCAAAGGAGTGGTGATGCACACATTTGGGGGTTATGCCAACAGCAAAGCT
GTCACACTGGAGGCTTCATTTACTCCAGACTCTCAGTTTATTATGATTGGTTCAGAGGAT
GGCAAGATCCATGTCTGGAATGGAGAGAGCGGTATAAAAGTAGCTGTGTTGGATGGTAAA
CACACAGGCCCGATTACCTGTTTGCAATTCAACCCCAAGTTCATGACTTTTGCCAGTGCG
TGTTCCAACATGGCCTTTTGGTTGCCCACCATTGATGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1227 Download

>KIEE1227  313 aa
MKLTDSVLRSFRVAKVFRENSDKINCFDFSPNGETVISSSDDDSIVLYDCQEGKPKRTLY
SKKYGVDLIRYTHAANTVVYSSNKIDDTIRYLSLHDNKYIRYFPGHSKRVVALSMSPVDD
TFISGSLDKTIRLWDLRSPNCQGLMHLQGKPVCSFDPEGLIFAAGVNSEMVKLYDLRSFD
KGPFATFKMQYDRTCEWTGLKFSNDGKLILISTNGSFIRLIDAFKGVVMHTFGGYANSKA
VTLEASFTPDSQFIMIGSEDGKIHVWNGESGIKVAVLDGKHTGPITCLQFNPKFMTFASA
CSNMAFWLPTIDD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1227 Download

>pFN21AE1227  5644 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGCTGACCGACAGCGTGTTGCGGAGCTTCCG
CGTCGCTAAGGTGTTCCGCGAAAACTCGGACAAGATTAACTGCTTCGATTTCAGCCCCAA
CGGCGAGACGGTCATCTCGAGTAGCGACGACGACTCCATCGTGCTCTATGACTGCCAGGA
GGGCAAACCAAAGAGAACCCTGTACAGTAAGAAATATGGTGTGGACCTCATCAGATACAC
TCATGCAGCAAACACAGTTGTTTACAGCTCTAACAAAATAGACGATACTATTCGTTACTT
GTCCTTGCATGACAACAAATACATCAGATACTTTCCTGGACATAGCAAAAGGGTGGTGGC
CTTGTCCATGTCACCTGTGGATGACACTTTCATTTCTGGGTCTCTTGATAAGACCATTCG
ACTCTGGGATCTCCGGTCTCCTAACTGCCAGGGCCTCATGCATCTGCAGGGGAAGCCAGT
TTGTTCTTTTGATCCAGAAGGGTTAATTTTCGCTGCAGGTGTCAACTCTGAAATGGTCAA
GCTTTATGACCTTCGTTCTTTTGATAAGGGGCCATTTGCTACCTTTAAGATGCAGTATGA
TCGAACTTGTGAGTGGACAGGACTTAAATTCAGCAATGATGGCAAGCTCATCCTCATTTC
CACCAACGGCAGCTTCATTCGTCTGATTGATGCATTCAAAGGAGTGGTGATGCACACATT
TGGGGGTTATGCCAACAGCAAAGCTGTCACACTGGAGGCTTCATTTACTCCAGACTCTCA
GTTTATTATGATTGGTTCAGAGGATGGCAAGATCCATGTCTGGAATGGAGAGAGCGGTAT
AAAAGTAGCTGTGTTGGATGGTAAACACACAGGCCCGATTACCTGTTTGCAATTCAACCC
CAAGTTCATGACTTTTGCCAGTGCGTGTTCCAACATGGCCTTTTGGTTGCCCACCATTGA
TGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT