COL9A2 ORF clone pFN21AE1179

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1179

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Clone info.

Species

human

Clone Name

pFN21AE1179 Sequence (with vector) Restriction Map

Product ID

FHC20248

ORF Sequence

DNA sequence (2067 bp)
Protein sequence (689 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590601]

Gene

KIEE1179

Symbol

COL9A2

Alias : DJ39G22.4, EDM2, MED, STL5

Description

collagen, type IX, alpha 2

Original Clone ID

cs03052

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2067 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 689 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS450.1 1.5e-112 99.9 COL9A2 CCDS47447.1 9.5e-58 55.4 COL9A1 CCDS4971.1 2.7e-57 56.1 COL9A1 CCDS13505.1 2.2e-50 50.8 COL9A3 CCDS6376.1 1.4e-40 45.4 COL22A1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR008160 26 81 PF01391 Collagen triple helix repeat IPR008160 60 115 PF01391 Collagen triple helix repeat IPR008160 116 162 PF01391 Collagen triple helix repeat IPR008160 179 235 PF01391 Collagen triple helix repeat IPR008160 460 517 PF01391 Collagen triple helix repeat IPR008160 550 608 PF01391 Collagen triple helix repeat IPR008160 612 662 PF01391 Collagen triple helix repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006710428 689 120260,600204,603932,614284 99.9 100.0 NP_001843 689 120260,600204,603932,614284 99.9 100.0 XP_016855821 693 120260,600204,603932,614284 99.3 100.0 XP_016855822 595 120260,600204,603932,614284 99.8 86.4 XP_011539017 599 120260,600204,603932,614284 99.2 86.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1179 Download

>KIEE1179  2067 bp
ATGGCCGCCGCTACGGCCTCCCCCCGCAGCCTCCTTGTTCTCCTCCAGGTGGTAGTGCTC
GCTCTGGCGCAGATTAGAGGTCCACCGGGAGAGCGGGGCCCCCCGGGTCCCCCGGGACCG
CCGGGAGTGCCTGGATCCGACGGCATCGACGGTGACAATGGGCCCCCTGGAAAAGCTGGC
CCTCCGGGACCCAAGGGCGAGCCTGGCAAAGCTGGGCCAGATGGGCCAGACGGGAAGCCC
GGGATTGATGGTTTAACTGGAGCCAAGGGGGAGCCTGGCCCCATGGGGATCCCTGGAGTC
AAGGGCCAGCCCGGGCTTCCTGGTCCTCCTGGCCTTCCGGGCCCTGGTTTTGCTGGACCT
CCTGGGCCTCCTGGACCTGTTGGCCTCCCTGGTGAGATTGGAATCCGAGGCCCCAAGGGG
GACCCTGGACCAGATGGACCATCGGGGCCCCCAGGACCCCCTGGGAAACCTGGTCGCCCG
GGAACCATCCAGGGTCTGGAAGGCAGTGCGGATTTCCTGTGTCCAACCAACTGTCCACCC
GGAATGAAAGGTCCCCCAGGGCTGCAGGGAGTGAAGGGGCATGCGGGCAAACGCGGGATT
CTGGGTGATCCTGGCCACCAGGGGAAGCCGGGTCCCAAGGGAGATGTGGGTGCCTCTGGA
GAGCAAGGCATCCCTGGACCACCGGGTCCCCAGGGCATCAGGGGCTACCCAGGCATGGCA
GGGCCCAAGGGAGAGACGGGCCCTCATGGATATAAAGGCATGGTGGGCGCTATCGGTGCC
ACTGGGCCACCGGGTGAGGAAGGTCCTAGGGGACCGCCAGGCCGAGCTGGGGAGAAGGGT
GACGAGGGCAGCCCAGGTATTCGTGGACCCCAGGGGATCACAGGCCCGAAAGGAGCAACG
GGCCCCCCAGGCATCAACGGCAAGGATGGGACCCCAGGCACGCCTGGCATGAAGGGCAGT
GCAGGACAGGCGGGACAGCCCGGAAGTCCAGGCCACCAGGGCGTAGCGGGTGTGCCAGGC
CAGCCTGGGACAAAAGGAGGCCCTGGAGACCAGGGTGAGCCGGGCCCGCAGGGCCTTCCT
GGATTCTCTGGTCCCCCTGGGAAAGAGGGAGAGCCAGGGCCTCGAGGAGAAATTGGTCCC
CAGGGCATCATGGGACAGAAGGGTGACCAAGGCGAGAGGGGTCCAGTGGGGCAACCAGGC
CCTCAGGGAAGGCAGGGCCCTAAGGGGGAGCAGGGCCCCCCCGGAATTCCAGGGCCCCAA
GGCTTGCCAGGCGTCAAAGGAGACAAGGGCTCCCCAGGGAAGACCGGGCCCCGCGGCAAA
GTGGGTGACCCAGGGGTGGCCGGCCTCCCCGGAGAGAAAGGCGAGAAGGGCGAGTCCGGC
GAGCCGGGGCCCAAGGGACAGCAAGGAGTACGTGGAGAACCCGGCTACCCTGGCCCCAGC
GGGGATGCGGGCGCCCCAGGGGTTCAGGGCTACCCTGGTCCCCCCGGCCCTCGAGGACTG
GCCGGGAACCGAGGCGTGCCAGGACAGCCCGGGAGACAGGGCGTGGAGGGCCGGGATGCC
ACTGACCAGCACATCGTGGATGTGGCGCTGAAGATGCTGCAAGAGCAACTGGCAGAGGTC
GCCGTGAGTGCCAAGCGGGAAGCCCTGGGTGCGGTGGGCATGATGGGTCCTCCAGGACCT
CCTGGGCCCCCTGGGTACCCAGGCAAGCAGGGCCCCCATGGGCACCCTGGCCCTCGGGGC
GTTCCTGGCATCGTGGGAGCCGTGGGTCAGATCGGCAACACGGGGCCCAAGGGAAAACGT
GGAGAGAAGGGTGATCCAGGAGAAGTGGGACGGGGGCACCCCGGGATGCCTGGGCCCCCA
GGGATCCCAGGACTCCCTGGCCGGCCTGGCCAGGCAATCAACGGCAAGGATGGAGATCGA
GGGTCCCCAGGGGCTCCAGGAGAGGCAGGTCGACCTGGCCTGCCAGGCCCCGTGGGGCTG
CCGGGCTTCTGTGAACCTGCCGCCTGCCTTGGAGCTTCGGCCTATGCCTCTGCCCGCCTT
ACAGAGCCTGGATCCATCAAGGGGCCT

ORF Protein

Cloned ORF protein sequence for pFN21AE1179 Download

>KIEE1179  689 aa
MAAATASPRSLLVLLQVVVLALAQIRGPPGERGPPGPPGPPGVPGSDGIDGDNGPPGKAG
PPGPKGEPGKAGPDGPDGKPGIDGLTGAKGEPGPMGIPGVKGQPGLPGPPGLPGPGFAGP
PGPPGPVGLPGEIGIRGPKGDPGPDGPSGPPGPPGKPGRPGTIQGLEGSADFLCPTNCPP
GMKGPPGLQGVKGHAGKRGILGDPGHQGKPGPKGDVGASGEQGIPGPPGPQGIRGYPGMA
GPKGETGPHGYKGMVGAIGATGPPGEEGPRGPPGRAGEKGDEGSPGIRGPQGITGPKGAT
GPPGINGKDGTPGTPGMKGSAGQAGQPGSPGHQGVAGVPGQPGTKGGPGDQGEPGPQGLP
GFSGPPGKEGEPGPRGEIGPQGIMGQKGDQGERGPVGQPGPQGRQGPKGEQGPPGIPGPQ
GLPGVKGDKGSPGKTGPRGKVGDPGVAGLPGEKGEKGESGEPGPKGQQGVRGEPGYPGPS
GDAGAPGVQGYPGPPGPRGLAGNRGVPGQPGRQGVEGRDATDQHIVDVALKMLQEQLAEV
AVSAKREALGAVGMMGPPGPPGPPGYPGKQGPHGHPGPRGVPGIVGAVGQIGNTGPKGKR
GEKGDPGEVGRGHPGMPGPPGIPGLPGRPGQAINGKDGDRGSPGAPGEAGRPGLPGPVGL
PGFCEPAACLGASAYASARLTEPGSIKGP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1179 Download

>pFN21AE1179  6772 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGCCGCTACGGCCTCCCCCCGCAGCCTCCT
TGTTCTCCTCCAGGTGGTAGTGCTCGCTCTGGCGCAGATTAGAGGTCCACCGGGAGAGCG
GGGCCCCCCGGGTCCCCCGGGACCGCCGGGAGTGCCTGGATCCGACGGCATCGACGGTGA
CAATGGGCCCCCTGGAAAAGCTGGCCCTCCGGGACCCAAGGGCGAGCCTGGCAAAGCTGG
GCCAGATGGGCCAGACGGGAAGCCCGGGATTGATGGTTTAACTGGAGCCAAGGGGGAGCC
TGGCCCCATGGGGATCCCTGGAGTCAAGGGCCAGCCCGGGCTTCCTGGTCCTCCTGGCCT
TCCGGGCCCTGGTTTTGCTGGACCTCCTGGGCCTCCTGGACCTGTTGGCCTCCCTGGTGA
GATTGGAATCCGAGGCCCCAAGGGGGACCCTGGACCAGATGGACCATCGGGGCCCCCAGG
ACCCCCTGGGAAACCTGGTCGCCCGGGAACCATCCAGGGTCTGGAAGGCAGTGCGGATTT
CCTGTGTCCAACCAACTGTCCACCCGGAATGAAAGGTCCCCCAGGGCTGCAGGGAGTGAA
GGGGCATGCGGGCAAACGCGGGATTCTGGGTGATCCTGGCCACCAGGGGAAGCCGGGTCC
CAAGGGAGATGTGGGTGCCTCTGGAGAGCAAGGCATCCCTGGACCACCGGGTCCCCAGGG
CATCAGGGGCTACCCAGGCATGGCAGGGCCCAAGGGAGAGACGGGCCCTCATGGATATAA
AGGCATGGTGGGCGCTATCGGTGCCACTGGGCCACCGGGTGAGGAAGGTCCTAGGGGACC
GCCAGGCCGAGCTGGGGAGAAGGGTGACGAGGGCAGCCCAGGTATTCGTGGACCCCAGGG
GATCACAGGCCCGAAAGGAGCAACGGGCCCCCCAGGCATCAACGGCAAGGATGGGACCCC
AGGCACGCCTGGCATGAAGGGCAGTGCAGGACAGGCGGGACAGCCCGGAAGTCCAGGCCA
CCAGGGCGTAGCGGGTGTGCCAGGCCAGCCTGGGACAAAAGGAGGCCCTGGAGACCAGGG
TGAGCCGGGCCCGCAGGGCCTTCCTGGATTCTCTGGTCCCCCTGGGAAAGAGGGAGAGCC
AGGGCCTCGAGGAGAAATTGGTCCCCAGGGCATCATGGGACAGAAGGGTGACCAAGGCGA
GAGGGGTCCAGTGGGGCAACCAGGCCCTCAGGGAAGGCAGGGCCCTAAGGGGGAGCAGGG
CCCCCCCGGAATTCCAGGGCCCCAAGGCTTGCCAGGCGTCAAAGGAGACAAGGGCTCCCC
AGGGAAGACCGGGCCCCGCGGCAAAGTGGGTGACCCAGGGGTGGCCGGCCTCCCCGGAGA
GAAAGGCGAGAAGGGCGAGTCCGGCGAGCCGGGGCCCAAGGGACAGCAAGGAGTACGTGG
AGAACCCGGCTACCCTGGCCCCAGCGGGGATGCGGGCGCCCCAGGGGTTCAGGGCTACCC
TGGTCCCCCCGGCCCTCGAGGACTGGCCGGGAACCGAGGCGTGCCAGGACAGCCCGGGAG
ACAGGGCGTGGAGGGCCGGGATGCCACTGACCAGCACATCGTGGATGTGGCGCTGAAGAT
GCTGCAAGAGCAACTGGCAGAGGTCGCCGTGAGTGCCAAGCGGGAAGCCCTGGGTGCGGT
GGGCATGATGGGTCCTCCAGGACCTCCTGGGCCCCCTGGGTACCCAGGCAAGCAGGGCCC
CCATGGGCACCCTGGCCCTCGGGGCGTTCCTGGCATCGTGGGAGCCGTGGGTCAGATCGG
CAACACGGGGCCCAAGGGAAAACGTGGAGAGAAGGGTGATCCAGGAGAAGTGGGACGGGG
GCACCCCGGGATGCCTGGGCCCCCAGGGATCCCAGGACTCCCTGGCCGGCCTGGCCAGGC
AATCAACGGCAAGGATGGAGATCGAGGGTCCCCAGGGGCTCCAGGAGAGGCAGGTCGACC
TGGCCTGCCAGGCCCCGTGGGGCTGCCGGGCTTCTGTGAACCTGCCGCCTGCCTTGGAGC
TTCGGCCTATGCCTCTGCCCGCCTTACAGAGCCTGGATCCATCAAGGGGCCTGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT