CYTH1 ORF clone pFN21AE1139

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Halotag ORF Clone pFN21AE1139

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Clone info.

Species

human

Clone Name

pFN21AE1139 Sequence (with vector) Restriction Map

Product ID

FHC28510

ORF Sequence

DNA sequence (1191 bp)
Protein sequence (397 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590939]

Gene

KIEE1139

Symbol

CYTH1

Alias : B2-1, CYTOHESIN-1, D17S811E, PSCD1, SEC7

Description

cytohesin 1, transcript variant 2

Original Clone ID

cs03012

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1191 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 397 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32754.1 2.4e-136 100.0 CYTH1 CCDS42392.2 1e-135 99.7 CYTH1 CCDS5346.1 1.5e-115 84.2 CYTH3 CCDS12722.1 2.4e-115 83.0 CYTH2 CCDS13946.1 7.8e-97 70.5 CYTH4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000904 62 244 PF01369 SEC7-like IPR001849 263 376 PF00169 Pleckstrin homology domain HMMSmart IPR000904 59 244 SM00222 SEC7-like IPR001849 261 378 SM00233 Pleckstrin homology domain ProfileScan IPR000904 55 242 PS50190 SEC7-like IPR001849 260 376 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_059430 397 182115 100.0 100.0 NP_004753 398 182115 99.7 100.0 XP_016880825 399 182115 99.2 99.7 XP_016880826 380 182115 99.2 94.7 XP_016880827 338 182115 100.0 85.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1139 Download

>KIEE1139  1191 bp
ATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGACAGCAGAGGAGCGTCAAGAACTG
GAGAACATCCGACGGAGAAAACAGGAGCTGCTGGCTGACATTCAGAGGCTGAAGGATGAG
ATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGGATCCACAGAGGAAAGGAAAAACATG
CAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAAATTTAATATGGACCCTAAAAAGGGG
ATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACACTTGTGAAGACATTGCCCAGTTC
TTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGGCGACTACCTAGGGGAGAGAGAT
GAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCTGCATGAGTTCACTGATCTTAAT
CTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCGGCTACCCGGAGAGGCCCAGAAG
ATCGACCGGATGATGGAGGCGTTTGCCCAGCGATATTGTCAGTGCAATAATGGCGTGTTC
CAGTCCACGGATACTTGTTACGTCCTCTCCTTTGCCATCATCATGTTGAACACCAGTCTG
CACAACCCCAATGTCAAAGATAAGCCCACTGTGGAGAGGTTCATTGCCATGAACCGAGGC
ATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCGGAATCTCTATGAGAGCATAAAA
AATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGACCTCACTCACACTTTCTTCAAT
CCAGACCGAGAAGGCTGGCTATTGAAACTCGGTGGCAGGGTAAAGACTTGGAAGAGACGC
TGGTTCATTCTGACTGACAACTGCCTTTACTACTTTGAGTATACCACGGATAAGGAGCCC
CGTGGAATCATCCCTTTAGAGAATCTGAGTATCCGGGAAGTGGAGGACTCCAAAAAACCA
AACTGCTTTGAGCTTTATATCCCCGACAATAAAGACCAAGTTATCAAGGCCTGCAAGACC
GAGGCTGACGGGCGGGTGGTGGAGGGGAACCACACTGTTTACCGGATCTCAGCTCCGACG
CCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAAAGCAGCCATCAGCAGGGACCCTTTC
TACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTCCTCCACGAAGCGACAC

ORF Protein

Cloned ORF protein sequence for pFN21AE1139 Download

>KIEE1139  397 aa
MEEDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKDEIAEVANEIENLGSTEERKNM
QRNKQVAMGRKKFNMDPKKGIQFLIENDLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERD
EFNIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNNGVF
QSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNRGINDGGDLPEELLRNLYESIK
NEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEP
RGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGNHTVYRISAPT
PEEKEEWIKCIKAAISRDPFYEMLAARKKKVSSTKRH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1139 Download

>pFN21AE1139  5896 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGAGGACGACAGCTACGTTCCCAGTGACCT
GACAGCAGAGGAGCGTCAAGAACTGGAGAACATCCGACGGAGAAAACAGGAGCTGCTGGC
TGACATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGAAATTGAAAACCTGGG
ATCCACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGGAAAAA
ATTTAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAA
CACTTGTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCAT
CGGCGACTACCTAGGGGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGA
GCTGCATGAGTTCACTGATCTTAATCTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTT
CCGGCTACCCGGAGAGGCCCAGAAGATCGACCGGATGATGGAGGCGTTTGCCCAGCGATA
TTGTCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTACGTCCTCTCCTTTGC
CATCATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACTGTGGA
GAGGTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCT
CCGGAATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAA
TGACCTCACTCACACTTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCGGTGG
CAGGGTAAAGACTTGGAAGAGACGCTGGTTCATTCTGACTGACAACTGCCTTTACTACTT
TGAGTATACCACGGATAAGGAGCCCCGTGGAATCATCCCTTTAGAGAATCTGAGTATCCG
GGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATATCCCCGACAATAAAGA
CCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAACCACAC
TGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAA
AGCAGCCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTC
CTCCACGAAGCGACACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT