WDR45 ORF clone pFN21AE1095

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1095

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Clone info.

Species

human

Clone Name

pFN21AE1095 Sequence (with vector) Restriction Map

Product ID

FHC07866

Flexi Clone PID

FXC07866

ORF Sequence

DNA sequence (1080 bp)
Protein sequence (360 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587560]

Gene

KIEE1095

Symbol

WDR45

Alias : JM5, NBIA4, NBIA5, WDRX1, WIPI-4, WIPI4

Description

WD repeat domain 45, transcript variant 2

Original Clone ID

oc00674

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1080 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 360 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35250.1 4.4e-154 100.0 WDR45 CCDS14318.1 2.7e-153 99.7 WDR45 CCDS11815.2 1.7e-50 47.9 WDR45B CCDS82193.1 2.2e-17 35.7 WIPI1 CCDS11677.1 2.7e-17 30.3 WIPI1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 187 215 PF00400 WD40 repeat IPR019781 239 264 PF00400 WD40 repeat HMMSmart IPR001680 1 33 SM00320 WD40 repeat IPR001680 92 127 SM00320 WD40 repeat IPR001680 181 221 SM00320 WD40 repeat IPR001680 224 265 SM00320 WD40 repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001025067 360 300526,300894 100.0 100.0 NP_009006 361 300526,300894 99.7 100.0 XP_005256434 310 609226 43.3 97.2 NP_062559 344 609226 47.9 97.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1095 Download

>KIEE1095  1080 bp
ATGACTCAACAGCCACTTCGAGGAGTGACCAGCCTGCGTTTCAACCAAGACCAAAGCTGC
TTTTGCTGCGCCATGGAGACAGGTGTGCGCATCTACAACGTGGAGCCCTTGATGGAGAAG
GGGCATCTGGACCACGAGCAGGTGGGCAGCATGGGCTTGGTGGAGATGCTGCACCGCTCC
AACCTTCTGGCCTTGGTGGGCGGTGGTAGTAGTCCCAAGTTCTCAGAGATCTCAGTGCTG
ATCTGGGACGATGCCCGGGAGGGCAAGGACTCCAAGGAGAAGCTGGTGCTGGAGTTCACC
TTCACCAAGCCAGTGCTTTCTGTGCGCATGCGCCATGACAAGATCGTGATCGTGCTGAAG
AACCGCATCTATGTGTACTCCTTCCCCGACAATCCCCGAAAGCTGTTTGAGTTTGATACC
CGGGACAACCCCAAGGGGCTCTGTGACCTCTGCCCCAGCCTGGAGAAGCAACTGCTAGTG
TTCCCGGGACACAAGTGTGGGAGTCTGCAACTTGTGGACCTGGCGAGCACAAAGCCTGGC
ACCTCGTCTGCTCCATTCACGATCAATGCACATCAGAGTGACATAGCCTGTGTGTCTCTA
AACCAGCCAGGCACTGTAGTGGCCTCAGCCTCCCAGAAGGGTACCCTTATTCGCCTCTTT
GACACACAATCCAAGGAGAAACTGGTGGAGCTGCGCCGAGGCACTGACCCTGCCACCCTC
TACTGCATTAACTTCAGCCACGACTCCTCCTTCCTCTGCGCTTCCAGTGATAAGGGTACT
GTCCATATCTTTGCTCTCAAGGATACCCGCCTCAACCGCCGCTCCGCGCTGGCTCGCGTG
GGCAAGGTGGGGCCTATGATTGGGCAGTACGTGGACTCTCAGTGGAGCCTGGCGAGCTTC
ACTGTGCCTGCTGAGTCAGCTTGCATCTGCGCCTTCGGTCGCAATACTTCCAAGAACGTC
AACTCTGTCATTGCCATCTGCGTAGATGGGACCTTCCACAAATATGTCTTCACTCCTGAT
GGAAACTGCAACAGAGAGGCTTTCGACGTGTACCTTGACATCTGTGATGATGATGACTTT

ORF Protein

Cloned ORF protein sequence for pFN21AE1095 Download

>KIEE1095  360 aa
MTQQPLRGVTSLRFNQDQSCFCCAMETGVRIYNVEPLMEKGHLDHEQVGSMGLVEMLHRS
NLLALVGGGSSPKFSEISVLIWDDAREGKDSKEKLVLEFTFTKPVLSVRMRHDKIVIVLK
NRIYVYSFPDNPRKLFEFDTRDNPKGLCDLCPSLEKQLLVFPGHKCGSLQLVDLASTKPG
TSSAPFTINAHQSDIACVSLNQPGTVVASASQKGTLIRLFDTQSKEKLVELRRGTDPATL
YCINFSHDSSFLCASSDKGTVHIFALKDTRLNRRSALARVGKVGPMIGQYVDSQWSLASF
TVPAESACICAFGRNTSKNVNSVIAICVDGTFHKYVFTPDGNCNREAFDVYLDICDDDDF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1095 Download

>pFN21AE1095  5785 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACTCAACAGCCACTTCGAGGAGTGACCAGCCT
GCGTTTCAACCAAGACCAAAGCTGCTTTTGCTGCGCCATGGAGACAGGTGTGCGCATCTA
CAACGTGGAGCCCTTGATGGAGAAGGGGCATCTGGACCACGAGCAGGTGGGCAGCATGGG
CTTGGTGGAGATGCTGCACCGCTCCAACCTTCTGGCCTTGGTGGGCGGTGGTAGTAGTCC
CAAGTTCTCAGAGATCTCAGTGCTGATCTGGGACGATGCCCGGGAGGGCAAGGACTCCAA
GGAGAAGCTGGTGCTGGAGTTCACCTTCACCAAGCCAGTGCTTTCTGTGCGCATGCGCCA
TGACAAGATCGTGATCGTGCTGAAGAACCGCATCTATGTGTACTCCTTCCCCGACAATCC
CCGAAAGCTGTTTGAGTTTGATACCCGGGACAACCCCAAGGGGCTCTGTGACCTCTGCCC
CAGCCTGGAGAAGCAACTGCTAGTGTTCCCGGGACACAAGTGTGGGAGTCTGCAACTTGT
GGACCTGGCGAGCACAAAGCCTGGCACCTCGTCTGCTCCATTCACGATCAATGCACATCA
GAGTGACATAGCCTGTGTGTCTCTAAACCAGCCAGGCACTGTAGTGGCCTCAGCCTCCCA
GAAGGGTACCCTTATTCGCCTCTTTGACACACAATCCAAGGAGAAACTGGTGGAGCTGCG
CCGAGGCACTGACCCTGCCACCCTCTACTGCATTAACTTCAGCCACGACTCCTCCTTCCT
CTGCGCTTCCAGTGATAAGGGTACTGTCCATATCTTTGCTCTCAAGGATACCCGCCTCAA
CCGCCGCTCCGCGCTGGCTCGCGTGGGCAAGGTGGGGCCTATGATTGGGCAGTACGTGGA
CTCTCAGTGGAGCCTGGCGAGCTTCACTGTGCCTGCTGAGTCAGCTTGCATCTGCGCCTT
CGGTCGCAATACTTCCAAGAACGTCAACTCTGTCATTGCCATCTGCGTAGATGGGACCTT
CCACAAATATGTCTTCACTCCTGATGGAAACTGCAACAGAGAGGCTTTCGACGTGTACCT
TGACATCTGTGATGATGATGACTTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT