MRGPRG ORF clone pFN21AE0804

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Halotag ORF Clone pFN21AE0804

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Clone info.

Species

human

Clone Name

pFN21AE0804 Sequence (with vector) Restriction Map

Product ID

FHC11278

Flexi Clone PID

FXC11278

ORF Sequence

DNA sequence (867 bp)
Protein sequence (289 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529240]

Gene

KIEE0804

Symbol

MRGPRG

Alias : GPR169, MRGG

Description

MAS-related GPR, member G

Original Clone ID

cp01204

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 867 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 289 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44520.1 1.1e-114 100.0 MRGPRG CCDS41603.2 4.7e-30 38.8 MRGPRE CCDS7846.1 4.4e-27 38.5 MRGPRX1 CCDS7830.1 1.3e-24 36.2 MRGPRX3 CCDS7831.1 2.7e-24 37.4 MRGPRX4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 14 38 PR00237 GPCR IPR000276 46 67 PR00237 GPCR IPR000276 85 107 PR00237 GPCR IPR000276 232 258 PR00237 GPCR HMMPfam IPR000276 29 250 PF00001 GPCR ProfileScan IPR017452 29 250 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 15 VFYLTLIVGLGGPVGNGLVLWNL 37 PRIMARY 23 2 49 YLLHLAAADFLFLSCRVGFSVAQ 71 SECONDARY 23 3 79 TLYFVLTFLWFAVGLWLLAAFSV 101 PRIMARY 23 4 122 SAVLCALVWTPTLPAVPLPANAC 144 SECONDARY 23 5 165 TWFLVLARVAWTAGVVLFVWVTC 187 PRIMARY 23 6 196 LYGIVLGALLLLFFCGLPSVFYW 218 PRIMARY 23 7 222 PLLNFLLPVFSPLATLLACVNSS 244 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001157849 289 607234 100.0 100.0 NP_001034254 312 607232 38.8 96.5 NP_671732 322 607227 38.5 94.1 XP_011518184 322 607229 36.2 94.1 NP_473372 322 607229 36.2 94.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0804 Download

>KIEE0804  867 bp
ATGTTTGGGCTGTTCGGCCTCTGGAGAACCTTCGACAGTGTGGTCTTCTACCTGACGCTG
ATCGTGGGCCTCGGGGGACCGGTAGGTAACGGGCTGGTGCTCTGGAACCTCGGCTTCCGC
ATCAAGAAGGGCCCCTTCTCCATCTACCTGCTGCACCTGGCCGCCGCCGACTTCCTGTTC
CTCTCCTGCCGTGTGGGCTTCTCCGTGGCTCAGGCTGCCCTGGGCGCCCAGGACACACTC
TACTTCGTGCTCACCTTCCTGTGGTTCGCGGTGGGGCTCTGGCTGCTGGCGGCCTTCAGC
GTGGAGCGCTGCCTCTCCGACCTCTTCCCCGCCTGCTACCAGGGCTGCCGGCCCAGACAC
GCCTCGGCCGTCCTCTGCGCCCTGGTGTGGACCCCGACCCTGCCGGCCGTGCCGCTGCCC
GCCAACGCCTGCGGCCTGCTGCGCAACAGCGCGTGCCCCCTGGTCTGCCCGCGCTACCAC
GTGGCCAGCGTCACCTGGTTCCTGGTGCTGGCCCGCGTCGCCTGGACGGCTGGCGTGGTC
CTCTTTGTCTGGGTGACCTGCTGCTCCACTCGCCCGCGGCCCAGGCTCTACGGCATCGTC
CTGGGCGCGCTGCTCCTGCTCTTCTTCTGTGGCCTGCCCTCGGTCTTCTACTGGAGCCTG
CAGCCCCTGCTGAACTTCCTGCTGCCCGTGTTTTCCCCGCTGGCCACGCTGCTGGCCTGC
GTCAACAGCAGCTCCAAGCCCCTCATCTACTCGGGGTTGGGCCGACAGCCCGGGAAGCGG
GAGCCGCTGAGGTCGGTACTGCGGAGGGCCCTGGGGGAGGGCGCCGAGCTGGGTGCCAGG
GGACAGTCCCTGCCCATGGGTCTCCTA

ORF Protein

Cloned ORF protein sequence for pFN21AE0804 Download

>KIEE0804  289 aa
MFGLFGLWRTFDSVVFYLTLIVGLGGPVGNGLVLWNLGFRIKKGPFSIYLLHLAAADFLF
LSCRVGFSVAQAALGAQDTLYFVLTFLWFAVGLWLLAAFSVERCLSDLFPACYQGCRPRH
ASAVLCALVWTPTLPAVPLPANACGLLRNSACPLVCPRYHVASVTWFLVLARVAWTAGVV
LFVWVTCCSTRPRPRLYGIVLGALLLLFFCGLPSVFYWSLQPLLNFLLPVFSPLATLLAC
VNSSSKPLIYSGLGRQPGKREPLRSVLRRALGEGAELGARGQSLPMGLL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0804 Download

>pFN21AE0804  5572 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTTGGGCTGTTCGGCCTCTGGAGAACCTTCGA
CAGTGTGGTCTTCTACCTGACGCTGATCGTGGGCCTCGGGGGACCGGTAGGTAACGGGCT
GGTGCTCTGGAACCTCGGCTTCCGCATCAAGAAGGGCCCCTTCTCCATCTACCTGCTGCA
CCTGGCCGCCGCCGACTTCCTGTTCCTCTCCTGCCGTGTGGGCTTCTCCGTGGCTCAGGC
TGCCCTGGGCGCCCAGGACACACTCTACTTCGTGCTCACCTTCCTGTGGTTCGCGGTGGG
GCTCTGGCTGCTGGCGGCCTTCAGCGTGGAGCGCTGCCTCTCCGACCTCTTCCCCGCCTG
CTACCAGGGCTGCCGGCCCAGACACGCCTCGGCCGTCCTCTGCGCCCTGGTGTGGACCCC
GACCCTGCCGGCCGTGCCGCTGCCCGCCAACGCCTGCGGCCTGCTGCGCAACAGCGCGTG
CCCCCTGGTCTGCCCGCGCTACCACGTGGCCAGCGTCACCTGGTTCCTGGTGCTGGCCCG
CGTCGCCTGGACGGCTGGCGTGGTCCTCTTTGTCTGGGTGACCTGCTGCTCCACTCGCCC
GCGGCCCAGGCTCTACGGCATCGTCCTGGGCGCGCTGCTCCTGCTCTTCTTCTGTGGCCT
GCCCTCGGTCTTCTACTGGAGCCTGCAGCCCCTGCTGAACTTCCTGCTGCCCGTGTTTTC
CCCGCTGGCCACGCTGCTGGCCTGCGTCAACAGCAGCTCCAAGCCCCTCATCTACTCGGG
GTTGGGCCGACAGCCCGGGAAGCGGGAGCCGCTGAGGTCGGTACTGCGGAGGGCCCTGGG
GGAGGGCGCCGAGCTGGGTGCCAGGGGACAGTCCCTGCCCATGGGTCTCCTAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT