STAP1 ORF clone pFN21AE0709

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Halotag ORF Clone pFN21AE0709

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Clone info.

Species

human

Clone Name

pFN21AE0709 Sequence (with vector) Restriction Map

Product ID

FHC11246

Flexi Clone PID

FXC11246

ORF Sequence

DNA sequence (885 bp)
Protein sequence (295 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528919]

Gene

KIEE0709

Symbol

STAP1

Alias : BRDG1, STAP-1

Description

signal transducing adaptor family member 1

Original Clone ID

cs02209

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 885 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 295 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3515.1 2e-122 100.0 STAP1 CCDS45926.1 2.3e-15 33.9 STAP2 CCDS12128.1 2.5e-15 33.9 STAP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000980 181 241 PF00017 SH2 motif HMMSmart IPR001849 26 123 SM00233 Pleckstrin homology domain IPR000980 175 262 SM00252 SH2 motif ProfileScan IPR001849 25 121 PS50003 Pleckstrin homology domain IPR000980 182 280 PS50001 SH2 motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036240 295 604298 100.0 100.0 NP_001304698 295 604298 100.0 100.0 NP_001013863 403 607881 33.9 91.5 XP_011526425 448 607881 33.9 91.5 NP_060190 449 607881 33.9 91.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0709 Download

>KIEE0709  885 bp
ATGATGGCTAAGAAGCCCCCAAAACCAGCCCCTCGCAGGATCTTCCAGGAAAGGTTAAAG
ATTACTGCTCTACCTTTGTACTTTGAAGGTTTTTTATTAATCAAGCGGTCAGGATACCGG
GAGTATGAGCATTACTGGACAGAGTTGAGAGGAACTACTCTTTTCTTTTATACCGACAAA
AAGAGTATAATATATGTTGACAAATTAGACATAGTAGACCTCACATGCCTTACTGAGCAG
AATTCAACTGAAAAGAACTGTGCGAAATTCACCCTTGTTTTGCCGAAAGAGGAAGTACAA
CTGAAGACAGAGAACACAGAAAGTGGGGAAGAATGGAGAGGCTTCATTCTTACAGTAACA
GAGCTGTCAGTTCCCCAAAACGTGTCACTCCTACCTGGGCAAGTAATTAAACTGCATGAA
GTCCTAGAGAGAGAAAAGAAAAGGAGGATTGAGACAGAGCAGAGTACGTCCGTGGAAAAA
GAGAAGGAACCAACTGAAGATTATGTGGATGTACTGAACCCTATGCCAGCATGTTTTTAT
ACAGTGTCCCGGAAAGAGGCAACTGAGATGCTCCAGAAGAACCCTTCTTTGGGAAATATG
ATCCTGAGGCCTGGTAGTGACAGTAGAAACTACTCCATCACTATTCGGCAGGAGATAGAC
ATTCCAAGAATCAAGCACTACAAAGTGATGAGCGTAGGACAAAACTACACTATTGAACTG
GAAAAACCTGTAACACTCCCAAACCTTTTCAGTGTCATTGATTATTTTGTGAAGGAGACT
CGAGGAAATTTAAGACCATTTATATGTTCAACTGATGAAAACACTGGTCAAGAACCCAGT
ATGGAAGGGAGAAGTGAAAAGTTGAAGAAAAATCCACACATTGCA

ORF Protein

Cloned ORF protein sequence for pFN21AE0709 Download

>KIEE0709  295 aa
MMAKKPPKPAPRRIFQERLKITALPLYFEGFLLIKRSGYREYEHYWTELRGTTLFFYTDK
KSIIYVDKLDIVDLTCLTEQNSTEKNCAKFTLVLPKEEVQLKTENTESGEEWRGFILTVT
ELSVPQNVSLLPGQVIKLHEVLEREKKRRIETEQSTSVEKEKEPTEDYVDVLNPMPACFY
TVSRKEATEMLQKNPSLGNMILRPGSDSRNYSITIRQEIDIPRIKHYKVMSVGQNYTIEL
EKPVTLPNLFSVIDYFVKETRGNLRPFICSTDENTGQEPSMEGRSEKLKKNPHIA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0709 Download

>pFN21AE0709  5590 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGGCTAAGAAGCCCCCAAAACCAGCCCCTCG
CAGGATCTTCCAGGAAAGGTTAAAGATTACTGCTCTACCTTTGTACTTTGAAGGTTTTTT
ATTAATCAAGCGGTCAGGATACCGGGAGTATGAGCATTACTGGACAGAGTTGAGAGGAAC
TACTCTTTTCTTTTATACCGACAAAAAGAGTATAATATATGTTGACAAATTAGACATAGT
AGACCTCACATGCCTTACTGAGCAGAATTCAACTGAAAAGAACTGTGCGAAATTCACCCT
TGTTTTGCCGAAAGAGGAAGTACAACTGAAGACAGAGAACACAGAAAGTGGGGAAGAATG
GAGAGGCTTCATTCTTACAGTAACAGAGCTGTCAGTTCCCCAAAACGTGTCACTCCTACC
TGGGCAAGTAATTAAACTGCATGAAGTCCTAGAGAGAGAAAAGAAAAGGAGGATTGAGAC
AGAGCAGAGTACGTCCGTGGAAAAAGAGAAGGAACCAACTGAAGATTATGTGGATGTACT
GAACCCTATGCCAGCATGTTTTTATACAGTGTCCCGGAAAGAGGCAACTGAGATGCTCCA
GAAGAACCCTTCTTTGGGAAATATGATCCTGAGGCCTGGTAGTGACAGTAGAAACTACTC
CATCACTATTCGGCAGGAGATAGACATTCCAAGAATCAAGCACTACAAAGTGATGAGCGT
AGGACAAAACTACACTATTGAACTGGAAAAACCTGTAACACTCCCAAACCTTTTCAGTGT
CATTGATTATTTTGTGAAGGAGACTCGAGGAAATTTAAGACCATTTATATGTTCAACTGA
TGAAAACACTGGTCAAGAACCCAGTATGGAAGGGAGAAGTGAAAAGTTGAAGAAAAATCC
ACACATTGCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT