DAPP1 ORF clone pFN21AE0689

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Halotag ORF Clone pFN21AE0689

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Clone info.

Species

human

Clone Name

pFN21AE0689 Sequence (with vector) Restriction Map

Product ID

FHC11234

Flexi Clone PID

FXC11234

ORF Sequence

DNA sequence (840 bp)
Protein sequence (280 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528911]

Gene

KIEE0689

Symbol

DAPP1

Alias : BAM32

Description

dual adaptor of phosphotyrosine and 3-phosphoinositides, transcript variant 1

Original Clone ID

cs02189

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 840 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 280 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS47112.1 4.9e-95 100.0 DAPP1 CCDS77945.1 1.1e-87 100.0 DAPP1 CCDS47243.1 8.6e-11 28.7 RASA1 CCDS34200.1 1e-10 28.7 RASA1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 35 49 PR00401 SH2 motif IPR000980 55 65 PR00401 SH2 motif IPR000980 67 78 PR00401 SH2 motif IPR000980 79 89 PR00401 SH2 motif IPR000980 98 112 PR00401 SH2 motif HMMPfam IPR000980 35 109 PF00017 SH2 motif IPR001849 167 255 PF00169 Pleckstrin homology domain HMMSmart IPR000980 33 115 SM00252 SH2 motif IPR001849 165 261 SM00233 Pleckstrin homology domain ProfileScan IPR000980 35 129 PS50001 SH2 motif IPR001849 164 259 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055210 280 605768 100.0 100.0 XP_011530142 267 605768 99.2 92.9 NP_001293080 263 605768 100.0 92.1 XP_016863512 258 605768 99.6 82.5 XP_016863513 258 605768 99.6 82.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0689 Download

>KIEE0689  840 bp
ATGGGCAGAGCAGAACTTCTAGAAGGGAAGATGAGCACCCAGGATCCCTCAGATCTGTGG
AGCAGATCCGATGGAGAGGCTGAGCTGCTCCAGGACTTGGGGTGGTATCACGGCAACCTC
ACACGCCATGCTGCTGAAGCTCTTCTCCTCTCAAATGGATGTGACGGCAGCTACCTTCTG
AGGGACAGCAATGAGACCACCGGGCTGTACTCTCTCTCTGTGAGGGCCAAAGATTCTGTT
AAACACTTTCATGTTGAATATACTGGATATTCATTTAAATTTGGCTTTAATGAATTCTCA
TCTTTGAAGGATTTTGTCAAGCATTTTGCAAATCAGCCTTTGATTGGAAGCGAGACAGGC
ACTCTGATGGTTCTAAAACATCCCTACCCAAGAAAAGTGGAAGAACCCTCCATTTATGAA
TCTGTCCGGGTTCACACAGCAATGCAGACAGGAAGAACAGAAGATGACCTTGTGCCCACA
GCACCTTCTCTGGGCACCAAAGAAGGTTACCTCACCAAACAGGGAGGCCTGGTCAAGACC
TGGAAAACAAGATGGTTTACTCTGCACAGGAATGAACTGAAATACTTCAAAGACCAGATG
TCACCAGAACCAATTCGGATCCTAGACCTAACAGAATGTTCAGCTGTACAATTCGATTAT
TCACAAGAAAGGGTAAACTGTTTTTGTTTGGTATTTCCATTCAGGACATTTTATCTCTGT
GCAAAGACAGGAGTAGAAGCTGATGAGTGGATCAAGATATTACGCTGGAAATTGTCACAA
ATAAGAAAACAGCTCAACCAAGGGGAAGGCACGATCCGATCTCGGTCGTTCATCTTTAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE0689 Download

>KIEE0689  280 aa
MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLLSNGCDGSYLL
RDSNETTGLYSLSVRAKDSVKHFHVEYTGYSFKFGFNEFSSLKDFVKHFANQPLIGSETG
TLMVLKHPYPRKVEEPSIYESVRVHTAMQTGRTEDDLVPTAPSLGTKEGYLTKQGGLVKT
WKTRWFTLHRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLC
AKTGVEADEWIKILRWKLSQIRKQLNQGEGTIRSRSFIFK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0689 Download

>pFN21AE0689  5545 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCAGAGCAGAACTTCTAGAAGGGAAGATGAG
CACCCAGGATCCCTCAGATCTGTGGAGCAGATCCGATGGAGAGGCTGAGCTGCTCCAGGA
CTTGGGGTGGTATCACGGCAACCTCACACGCCATGCTGCTGAAGCTCTTCTCCTCTCAAA
TGGATGTGACGGCAGCTACCTTCTGAGGGACAGCAATGAGACCACCGGGCTGTACTCTCT
CTCTGTGAGGGCCAAAGATTCTGTTAAACACTTTCATGTTGAATATACTGGATATTCATT
TAAATTTGGCTTTAATGAATTCTCATCTTTGAAGGATTTTGTCAAGCATTTTGCAAATCA
GCCTTTGATTGGAAGCGAGACAGGCACTCTGATGGTTCTAAAACATCCCTACCCAAGAAA
AGTGGAAGAACCCTCCATTTATGAATCTGTCCGGGTTCACACAGCAATGCAGACAGGAAG
AACAGAAGATGACCTTGTGCCCACAGCACCTTCTCTGGGCACCAAAGAAGGTTACCTCAC
CAAACAGGGAGGCCTGGTCAAGACCTGGAAAACAAGATGGTTTACTCTGCACAGGAATGA
ACTGAAATACTTCAAAGACCAGATGTCACCAGAACCAATTCGGATCCTAGACCTAACAGA
ATGTTCAGCTGTACAATTCGATTATTCACAAGAAAGGGTAAACTGTTTTTGTTTGGTATT
TCCATTCAGGACATTTTATCTCTGTGCAAAGACAGGAGTAGAAGCTGATGAGTGGATCAA
GATATTACGCTGGAAATTGTCACAAATAAGAAAACAGCTCAACCAAGGGGAAGGCACGAT
CCGATCTCGGTCGTTCATCTTTAAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT