MYLK4 ORF clone pFN21AE0656

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0656

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Clone info.

Species

human

Clone Name

pFN21AE0656 Sequence (with vector) Restriction Map

Product ID

FHC11219

Flexi Clone PID

FXC11219

ORF Sequence

DNA sequence (1164 bp)
Protein sequence (388 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528897]

Gene

KIEE0656

Symbol

MYLK4

Alias : SgK085

Description

myosin light chain kinase family, member 4

Original Clone ID

cs02156

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1164 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 388 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34330.1 2.7e-86 100.0 MYLK4 CCDS76861.1 4.8e-47 63.1 MYLK3 CCDS10723.2 6.5e-47 63.1 MYLK3 CCDS13191.1 3.1e-39 60.5 MYLK2 CCDS43141.1 1e-29 49.2 MYLK The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 108 361 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 106 361 SM00220 Serine/threonine-protein kinase domain IPR020635 106 334 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 106 361 PS50011 Protein kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006721397 478 612147 63.1 86.1 NP_001295230 478 612147 63.1 86.1 NP_872299 819 612147 63.1 86.1 XP_016861959 713 600922,613780 49.2 80.2 XP_016861960 714 600922,613780 49.2 80.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0656 Download

>KIEE0656  1164 bp
ATGTTAAAAGTGAAGAGGCTGGAAGAATTCAACACGTGTTATAACAGCAACCAGCTGGAG
AAAATGGCCTTTTTTCAGTGCAGGGAAGAGGTGGAGAAAGTGAAGTGTTTTCTGGAAAAA
AATTCTGGGGACCAGGATTCAAGATCTGGACATAATGAGGCGAAGGAGGTGTGGTCAAAC
GCCGACCTGACGGAAAGGATGCCCGTCAAAAGCAAAAGGACATCAGCCCTCGCAGTTGAC
ATCCCGGCTCCTCCGGCCCCATTTGATCATCGTATTGTGACAGCCAAGCAAGGAGCGGTC
AACAGCTTCTATACTGTGAGCAAGACAGAAATCCTAGGAGGAGGGCGTTTCGGCCAGGTT
CACAAGTGTGAGGAGACGGCCACAGGTCTGAAGCTGGCAGCCAAAATCATCAAGACCAGA
GGCATGAAGGACAAGGAGGAGGTGAAGAACGAGATCAGCGTCATGAACCAGCTGGACCAC
GCGAACCTCATCCAGCTGTACGATGCCTTCGAGTCTAAGAACGACATTGTCCTGGTCATG
GAGTATGTGGATGGTGGGGAGCTGTTTGACCGCATCATCGATGAGAGCTACAATTTGACG
GAGCTTGATACCATCCTGTTCATGAAGCAGATATGTGAGGGGATAAGGCACATGCATCAG
ATGTACATTCTCCACTTGGACCTGAAGCCTGAGAATATCCTGTGTGTGAATCGGGATGCT
AAGCAAATAAAAATTATTGATTTTGGATTGGCCAGAAGATACAAACCCAGAGAGAAGCTG
AAGGTGAACTTTGGAACCCCAGAATTTCTCGCCCCTGAAGTTGTGAACTATGATTTTGTT
TCATTTCCCACTGACATGTGGAGTGTGGGGGTCATCGCCTATATGCTACTTAGCGGTTTG
TCGCCTTTCCTGGGTGACAATGATGCTGAGACGCTGAACAACATCCTGGCCTGCAGGTGG
GACTTAGAGGATGAAGAATTTCAGGACATCTCGGAGGAGGCCAAGGAGTTCATCTCTAAG
CTTCTGATTAAGGAGAAGAGTTGGCGAATAAGTGCAAGCGAAGCTCTCAAGCACCCCTGG
TTGTCAGACCACAAGCTCCACTCCAGACTCAATGCCCAGAAGAAGAAGAATCGTGGCTCT
GATGCCCAGGACTTTGTGACCAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE0656 Download

>KIEE0656  388 aa
MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSGHNEAKEVWSN
ADLTERMPVKSKRTSALAVDIPAPPAPFDHRIVTAKQGAVNSFYTVSKTEILGGGRFGQV
HKCEETATGLKLAAKIIKTRGMKDKEEVKNEISVMNQLDHANLIQLYDAFESKNDIVLVM
EYVDGGELFDRIIDESYNLTELDTILFMKQICEGIRHMHQMYILHLDLKPENILCVNRDA
KQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFPTDMWSVGVIAYMLLSGL
SPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISASEALKHPW
LSDHKLHSRLNAQKKKNRGSDAQDFVTK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0656 Download

>pFN21AE0656  5869 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTAAAAGTGAAGAGGCTGGAAGAATTCAACAC
GTGTTATAACAGCAACCAGCTGGAGAAAATGGCCTTTTTTCAGTGCAGGGAAGAGGTGGA
GAAAGTGAAGTGTTTTCTGGAAAAAAATTCTGGGGACCAGGATTCAAGATCTGGACATAA
TGAGGCGAAGGAGGTGTGGTCAAACGCCGACCTGACGGAAAGGATGCCCGTCAAAAGCAA
AAGGACATCAGCCCTCGCAGTTGACATCCCGGCTCCTCCGGCCCCATTTGATCATCGTAT
TGTGACAGCCAAGCAAGGAGCGGTCAACAGCTTCTATACTGTGAGCAAGACAGAAATCCT
AGGAGGAGGGCGTTTCGGCCAGGTTCACAAGTGTGAGGAGACGGCCACAGGTCTGAAGCT
GGCAGCCAAAATCATCAAGACCAGAGGCATGAAGGACAAGGAGGAGGTGAAGAACGAGAT
CAGCGTCATGAACCAGCTGGACCACGCGAACCTCATCCAGCTGTACGATGCCTTCGAGTC
TAAGAACGACATTGTCCTGGTCATGGAGTATGTGGATGGTGGGGAGCTGTTTGACCGCAT
CATCGATGAGAGCTACAATTTGACGGAGCTTGATACCATCCTGTTCATGAAGCAGATATG
TGAGGGGATAAGGCACATGCATCAGATGTACATTCTCCACTTGGACCTGAAGCCTGAGAA
TATCCTGTGTGTGAATCGGGATGCTAAGCAAATAAAAATTATTGATTTTGGATTGGCCAG
AAGATACAAACCCAGAGAGAAGCTGAAGGTGAACTTTGGAACCCCAGAATTTCTCGCCCC
TGAAGTTGTGAACTATGATTTTGTTTCATTTCCCACTGACATGTGGAGTGTGGGGGTCAT
CGCCTATATGCTACTTAGCGGTTTGTCGCCTTTCCTGGGTGACAATGATGCTGAGACGCT
GAACAACATCCTGGCCTGCAGGTGGGACTTAGAGGATGAAGAATTTCAGGACATCTCGGA
GGAGGCCAAGGAGTTCATCTCTAAGCTTCTGATTAAGGAGAAGAGTTGGCGAATAAGTGC
AAGCGAAGCTCTCAAGCACCCCTGGTTGTCAGACCACAAGCTCCACTCCAGACTCAATGC
CCAGAAGAAGAAGAATCGTGGCTCTGATGCCCAGGACTTTGTGACCAAAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT