ELAVL3 ORF clone pFN21AE0620

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Halotag ORF Clone pFN21AE0620

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Clone info.

Species

human

Clone Name

pFN21AE0620 Sequence (with vector) Restriction Map

Product ID

FHC11205

Flexi Clone PID

FXC11205

ORF Sequence

DNA sequence (1080 bp)
Protein sequence (360 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB528883]

Gene

KIEE0620

Symbol

ELAVL3

Alias : HUC, HUCL, PLE21

Description

ELAV like neuron-specific RNA binding protein 3, transcript variant 2

Original Clone ID

cs02120

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1080 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 360 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45978.1 5.6e-106 100.0 ELAVL3 CCDS32912.1 7.7e-105 98.1 ELAVL3 CCDS44138.1 5.4e-87 82.2 ELAVL4 CCDS53315.1 5.5e-87 82.2 ELAVL4 CCDS44140.1 1e-86 81.5 ELAVL4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002343 39 54 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 96 111 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 112 129 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 130 145 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 145 157 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 186 203 PR00961 Paraneoplastic encephalomyelitis antigen HMMPfam IPR000504 41 111 PF00076 RNA recognition motif domain IPR000504 127 190 PF00076 RNA recognition motif domain IPR000504 279 348 PF00076 RNA recognition motif domain HMMSmart IPR003954 39 113 SM00361 RNA recognition motif domain IPR000504 40 113 SM00360 RNA recognition motif domain IPR000504 126 201 SM00360 RNA recognition motif domain IPR003954 278 351 SM00361 RNA recognition motif domain IPR000504 278 351 SM00360 RNA recognition motif domain HMMTigr IPR006548 36 359 TIGR01661 Splicing factor ELAV/HuD ProfileScan IPR000504 39 117 PS50102 RNA recognition motif domain IPR000504 125 205 PS50102 RNA recognition motif domain IPR000504 277 355 PS50102 RNA recognition motif domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115657 360 603458 100.0 100.0 NP_001411 367 603458 98.1 100.0 XP_011526080 366 603458 97.8 100.0 NP_001138248 366 168360 82.2 99.2 NP_001138249 369 168360 82.2 99.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0620 Download

>KIEE0620  1080 bp
ATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGCCCGGCCGGCCCG
GCCCTGCCCAACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGACAGCAAGACCAAC
CTCATCGTCAACTACCTGCCCCAGAACATGACCCAGGATGAGTTCAAGAGTCTCTTCGGC
AGCATTGGCGACATCGAGTCCTGCAAGTTGGTTCGGGACAAGATCACAGGGCAGAGCCTT
GGCTACGGGTTTGTGAACTATTCTGATCCCAATGATGCAGACAAAGCCATCAACACCCTC
AACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAGACCCAGTTCAGCA
TCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCATGAGCCAGAAAGAG
ATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCATCCTGGTGGACCAG
GTCACAGGTGTCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAGGATTGAGGCCGAA
GAGGCTATCAAAGGACTGAATGGGCAGAAGCCGCTGGGCGCAGCTGAGCCCATCACAGTC
AAGTTCGCGAACAACCCAAGTCAGAAGACGGGGCAGGCGCTGCTCACCCACCTCTACCAG
TCATCCGCCCGGCGCTACGCAGGCCCCCTACACCATCAGACCCAGCGTTTCCGGCTGGAC
AATTTGCTCAACATGGCCTACGGCGTCAAGAGGTTCTCGCCGATCGCCATCGATGGTATG
AGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGCGGCGGGCGCCGGCTGGTGCATCTTC
GTGTACAACCTGTCACCGGAGGCAGACGAGAGCGTGCTGTGGCAGCTGTTCGGGCCTTTT
GGGGCAGTCACCAACGTCAAGGTCATCCGTGATTTCACCACCAACAAGTGCAAGGGTTTC
GGCTTCGTGACCATGACCAACTATGACGAGGCGGCCATGGCCATCGCCAGCCTGAACGGC
TATCGCCTGGGCGAGCGCGTGCTGCAGGTCTCCTTCAAGACCAGCAAACAGCACAAGGCG

ORF Protein

Cloned ORF protein sequence for pFN21AE0620 Download

>KIEE0620  360 aa
MVTQILGAMESQVGGGPAGPALPNGPLLGTNGATDDSKTNLIVNYLPQNMTQDEFKSLFG
SIGDIESCKLVRDKITGQSLGYGFVNYSDPNDADKAINTLNGLKLQTKTIKVSYARPSSA
SIRDANLYVSGLPKTMSQKEMEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAE
EAIKGLNGQKPLGAAEPITVKFANNPSQKTGQALLTHLYQSSARRYAGPLHHQTQRFRLD
NLLNMAYGVKRFSPIAIDGMSGLAGVGLSGGAAGAGWCIFVYNLSPEADESVLWQLFGPF
GAVTNVKVIRDFTTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGERVLQVSFKTSKQHKA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0620 Download

>pFN21AE0620  5785 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTCACTCAGATACTGGGGGCCATGGAGTCTCA
GGTGGGGGGGGGCCCGGCCGGCCCGGCCCTGCCCAACGGGCCACTCCTTGGTACAAATGG
AGCCACTGACGACAGCAAGACCAACCTCATCGTCAACTACCTGCCCCAGAACATGACCCA
GGATGAGTTCAAGAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTGGTTCG
GGACAAGATCACAGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGATCCCAATGA
TGCAGACAAAGCCATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGT
GTCCTATGCCAGACCCAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCT
CCCCAAGACCATGAGCCAGAAAGAGATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCAT
CACGTCCCGCATCCTGGTGGACCAGGTCACAGGTGTCTCTCGGGGTGTGGGATTCATCCG
CTTTGACAAGAGGATTGAGGCCGAAGAGGCTATCAAAGGACTGAATGGGCAGAAGCCGCT
GGGCGCAGCTGAGCCCATCACAGTCAAGTTCGCGAACAACCCAAGTCAGAAGACGGGGCA
GGCGCTGCTCACCCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTACACCA
TCAGACCCAGCGTTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGGTT
CTCGCCGATCGCCATCGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGC
GGCGGGCGCCGGCTGGTGCATCTTCGTGTACAACCTGTCACCGGAGGCAGACGAGAGCGT
GCTGTGGCAGCTGTTCGGGCCTTTTGGGGCAGTCACCAACGTCAAGGTCATCCGTGATTT
CACCACCAACAAGTGCAAGGGTTTCGGCTTCGTGACCATGACCAACTATGACGAGGCGGC
CATGGCCATCGCCAGCCTGAACGGCTATCGCCTGGGCGAGCGCGTGCTGCAGGTCTCCTT
CAAGACCAGCAAACAGCACAAGGCGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT