GPR142 ORF clone pFN21AE0589

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Halotag ORF Clone pFN21AE0589

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Clone info.

Species

human

Clone Name

pFN21AE0589 Sequence (with vector) Restriction Map

Product ID

FHC11189

Flexi Clone PID

FXC11189

ORF Sequence

DNA sequence (1386 bp)
Protein sequence (462 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528868]

Gene

KIEE0589

Symbol

GPR142

Alias : GPRg1b, PGR2

Description

G protein-coupled receptor 142

Original Clone ID

oc01289

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1386 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 462 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11698.1 3.9e-150 100.0 GPR142 CCDS32398.1 2.7e-43 46.1 GPR139 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 158 182 PR00237 GPCR IPR000276 192 213 PR00237 GPCR IPR000276 238 260 PR00237 GPCR IPR000276 355 379 PR00237 GPCR IPR000276 396 422 PR00237 GPCR HMMPfam IPR000276 180 410 PF00001 GPCR ProfileScan IPR017452 173 414 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 157 VIPVIYYSVLLGLGLPVSLLTAV 179 PRIMARY 23 2 197 LALTASDIIIQVVIVFAGFLLQG 219 PRIMARY 23 3 240 AANHASVWIAILLTVDRYTALCH 262 SECONDARY 23 4 278 AIAAVLSAALLTGIPFYWWLDMW 300 PRIMARY 23 5 317 HCLTVYFIPCGVFLVTNSAIIHR 339 PRIMARY 23 6 358 ILLGITTLFTLLWAPRVFVMLYH 380 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_861455 462 609046 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0589 Download

>KIEE0589  1386 bp
ATGAGTATTATGATGTTGCCCATGGAGCAAAAGATCCAGTGGGTCCCCACTTCCCTGCAG
GACATCACTGCTGTCCTGGGTACAGAAGCATATACTGAGGAAGACAAATCAATGGTGTCC
CATGCACAGAAAAGCCAGCATTCTTGTCTCAGCCATTCCAGGTGGCTGAGGTCTCCACAG
GTCACAGGGGGAAGCTGGGACCTCCGAATAAGGCCATCCAAGGACTCCAGCAGTTTCCGC
CAGGCTCAGTGTCTGCGTAAGGATCCTGGGGCAAACAACCACTTGGAGAGCCAAGGGGTG
AGAGGTACAGCTGGCGATGCTGACAGGGAGCTGCGGGGACCCTCAGAAAAAGCCACAGCT
GGCCAGCCACGAGTGACCCTGCTGCCCACGCCCCACGTCAGCGGGCTGAGCCAGGAGTTT
GAAAGCCACTGGCCAGAGATCGCAGAGAGGTCCCCGTGTGTGGCTGGCGTCATCCCTGTC
ATCTACTACAGTGTCCTGCTGGGCTTGGGGCTGCCTGTCAGCCTCCTGACCGCAGTGGCC
CTGGCGCGCCTTGCCACCAGGACCAGGAGGCCCTCCTACTACTACCTTCTGGCGCTCACA
GCCTCGGATATCATCATCCAGGTGGTCATCGTGTTCGCGGGCTTCCTCCTGCAGGGAGCA
GTGCTGGCCCGCCAGGTGCCCCAGGCTGTGGTGCGCACGGCCAACATCCTGGAGTTTGCT
GCCAACCACGCCTCAGTCTGGATCGCCATCCTGCTCACGGTTGACCGCTACACTGCCCTG
TGCCACCCCCTGCACCATCGGGCCGCCTCGTCCCCAGGCCGGACCCGCCGGGCCATTGCT
GCTGTCCTGAGTGCTGCCCTGTTGACCGGCATCCCCTTCTACTGGTGGCTGGACATGTGG
AGAGACACCGACTCACCCAGAACACTGGACGAGGTCCTCAAGTGGGCTCACTGTCTCACT
GTCTATTTCATCCCTTGTGGCGTGTTCCTGGTCACCAACTCGGCCATCATCCACCGGCTA
CGGAGGAGGGGCCGGAGTGGGCTGCAGCCCCGGGTGGGCAAGAGCACAGCCATCCTCCTG
GGCATCACCACACTGTTCACCCTCCTGTGGGCGCCCCGGGTCTTCGTCATGCTCTACCAC
ATGTACGTGGCCCCTGTCCACCGGGACTGGAGGGTCCACCTGGCCTTGGATGTGGCCAAT
ATGGTGGCCATGCTCCACACGGCAGCCAACTTCGGCCTCTACTGCTTTGTCAGCAAGACT
TTCCGGGCCACTGTCCGACAGGTCATCCACGATGCCTACCTGCCCTGCACTTTGGCATCA
CAGCCAGAGGGCATGGCGGCGAAGCCTGTGATGGAGCCTCCGGGACTCCCCACAGGGGCA
GAAGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE0589 Download

>KIEE0589  462 aa
MSIMMLPMEQKIQWVPTSLQDITAVLGTEAYTEEDKSMVSHAQKSQHSCLSHSRWLRSPQ
VTGGSWDLRIRPSKDSSSFRQAQCLRKDPGANNHLESQGVRGTAGDADRELRGPSEKATA
GQPRVTLLPTPHVSGLSQEFESHWPEIAERSPCVAGVIPVIYYSVLLGLGLPVSLLTAVA
LARLATRTRRPSYYYLLALTASDIIIQVVIVFAGFLLQGAVLARQVPQAVVRTANILEFA
ANHASVWIAILLTVDRYTALCHPLHHRAASSPGRTRRAIAAVLSAALLTGIPFYWWLDMW
RDTDSPRTLDEVLKWAHCLTVYFIPCGVFLVTNSAIIHRLRRRGRSGLQPRVGKSTAILL
GITTLFTLLWAPRVFVMLYHMYVAPVHRDWRVHLALDVANMVAMLHTAANFGLYCFVSKT
FRATVRQVIHDAYLPCTLASQPEGMAAKPVMEPPGLPTGAEV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0589 Download

>pFN21AE0589  6091 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTATTATGATGTTGCCCATGGAGCAAAAGAT
CCAGTGGGTCCCCACTTCCCTGCAGGACATCACTGCTGTCCTGGGTACAGAAGCATATAC
TGAGGAAGACAAATCAATGGTGTCCCATGCACAGAAAAGCCAGCATTCTTGTCTCAGCCA
TTCCAGGTGGCTGAGGTCTCCACAGGTCACAGGGGGAAGCTGGGACCTCCGAATAAGGCC
ATCCAAGGACTCCAGCAGTTTCCGCCAGGCTCAGTGTCTGCGTAAGGATCCTGGGGCAAA
CAACCACTTGGAGAGCCAAGGGGTGAGAGGTACAGCTGGCGATGCTGACAGGGAGCTGCG
GGGACCCTCAGAAAAAGCCACAGCTGGCCAGCCACGAGTGACCCTGCTGCCCACGCCCCA
CGTCAGCGGGCTGAGCCAGGAGTTTGAAAGCCACTGGCCAGAGATCGCAGAGAGGTCCCC
GTGTGTGGCTGGCGTCATCCCTGTCATCTACTACAGTGTCCTGCTGGGCTTGGGGCTGCC
TGTCAGCCTCCTGACCGCAGTGGCCCTGGCGCGCCTTGCCACCAGGACCAGGAGGCCCTC
CTACTACTACCTTCTGGCGCTCACAGCCTCGGATATCATCATCCAGGTGGTCATCGTGTT
CGCGGGCTTCCTCCTGCAGGGAGCAGTGCTGGCCCGCCAGGTGCCCCAGGCTGTGGTGCG
CACGGCCAACATCCTGGAGTTTGCTGCCAACCACGCCTCAGTCTGGATCGCCATCCTGCT
CACGGTTGACCGCTACACTGCCCTGTGCCACCCCCTGCACCATCGGGCCGCCTCGTCCCC
AGGCCGGACCCGCCGGGCCATTGCTGCTGTCCTGAGTGCTGCCCTGTTGACCGGCATCCC
CTTCTACTGGTGGCTGGACATGTGGAGAGACACCGACTCACCCAGAACACTGGACGAGGT
CCTCAAGTGGGCTCACTGTCTCACTGTCTATTTCATCCCTTGTGGCGTGTTCCTGGTCAC
CAACTCGGCCATCATCCACCGGCTACGGAGGAGGGGCCGGAGTGGGCTGCAGCCCCGGGT
GGGCAAGAGCACAGCCATCCTCCTGGGCATCACCACACTGTTCACCCTCCTGTGGGCGCC
CCGGGTCTTCGTCATGCTCTACCACATGTACGTGGCCCCTGTCCACCGGGACTGGAGGGT
CCACCTGGCCTTGGATGTGGCCAATATGGTGGCCATGCTCCACACGGCAGCCAACTTCGG
CCTCTACTGCTTTGTCAGCAAGACTTTCCGGGCCACTGTCCGACAGGTCATCCACGATGC
CTACCTGCCCTGCACTTTGGCATCACAGCCAGAGGGCATGGCGGCGAAGCCTGTGATGGA
GCCTCCGGGACTCCCCACAGGGGCAGAAGTGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT