OTOP2 ORF clone pFN21AE0371

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0371

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Clone info.

Species

human

Clone Name

pFN21AE0371 Sequence (with vector) Restriction Map

Product ID

FHC06247

Flexi Clone PID

FXC06247

ORF Sequence

DNA sequence (1686 bp)
Protein sequence (562 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527196]

Gene

KIEE0371

Symbol

OTOP2

Description

otopetrin 2

Original Clone ID

oc01071

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1686 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 562 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11708.1 0 100.0 OTOP2 CCDS3372.1 1.5e-50 34.2 OTOP1 CCDS11709.1 6.7e-30 43.0 OTOP3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004878 102 219 PF03189 Otopetrin IPR004878 230 439 PF03189 Otopetrin IPR004878 484 548 PF03189 Otopetrin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 28 GRLLSVLLAVNVLLLACTLISGG 50 PRIMARY 23 2 58 YDTDVFALLTAMMLLATLWILFY 80 PRIMARY 23 3 101 IWLRGGLVLFGICTLIMDVFKTG 123 PRIMARY 23 4 134 AIKILHPLIQAVFVIIQTYFLWV 156 PRIMARY 23 5 165 HLDLTWCGLMFTLTTNLAIWMAA 187 SECONDARY 23 6 226 CLCSTAVCQIFQQGYFYLYPFNI 248 SECONDARY 23 7 291 AGPVLGLLLFVVGLAVFIIYEVQ 313 PRIMARY 23 8 325 LVIYYSFNIVCLGLTTLVSLSGS 347 SECONDARY 23 9 369 VALLMGAALGQYAISYYSIVAVV 391 SECONDARY 23 10 493 LKDISLFLLLCNVILWIMPAFGA 515 PRIMARY 23 11 526 DFYGYSLWAVIVNICLPFGIFYR 548 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_835454 562 607827 100.0 100.0 XP_011523781 562 607827 100.0 100.0 NP_819056 612 607806 34.2 97.7 XP_011523046 567 607828 43.0 95.4 NP_001258934 578 607828 43.0 95.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0371 Download

>KIEE0371  1686 bp
ATGTCCGAGGAGCTGGCCCAGGGCCCCAAGGAGAGCCCCCCGGCGCCGCGTGCGGGCCCC
AGGGAGGTGTGGAAGAAGGGTGGCCGCCTGCTGTCGGTGCTGCTGGCGGTGAACGTGCTG
CTCCTCGCCTGCACGCTCATCAGCGGCGGAGCCTTCAACAAGGTGGCCGTGTACGACACC
GACGTGTTCGCGCTGCTCACTGCGATGATGCTGCTGGCAACGCTCTGGATCCTCTTCTAC
CTCCTCCGAACCGTGCGCTGCCCCTGCGCGGTACCCTACCGGGACGCGCACGCTGGCCCC
ATCTGGCTCCGAGGTGGGCTGGTGCTGTTTGGAATCTGCACCCTCATCATGGATGTCTTC
AAGACCGGCTACTACTCCAGTTTCTTTGAGTGCCAGTCAGCCATCAAGATCCTGCACCCC
CTCATCCAGGCTGTGTTTGTCATCATCCAGACCTACTTTCTCTGGGTCTCTGCTAAAGAC
TGCGTTCACGTCCACCTGGATCTGACCTGGTGTGGTCTCATGTTCACACTCACCACCAAC
CTGGCCATCTGGATGGCGGCCGTGGTGGATGAATCTGTGCACCAATCCCACTCCTACAGC
AGTTCTCACAGCAACGCCAGCCACGCCCGTCTCATCTCTGACCAGCATGCAGACAACCCG
GTCGGAGGAGACTCCTGCCTCTGCAGCACGGCCGTCTGCCAGATCTTCCAGCAGGGGTAC
TTCTACCTATATCCCTTCAACATCGAGTACAGCCTCTTCGCCTCCACCATGCTGTATGTC
ATGTGGAAGAATGTGGGTAGATTCCTGGCCTCCACCCCTGGCCACAGCCACACCCCAACC
CCTGTCAGCCTCTTCCGGGAGACCTTTTTTGCTGGCCCGGTTCTGGGCCTGCTGCTCTTC
GTGGTGGGGCTGGCTGTCTTCATCATCTACGAGGTTCAAGTGAGCGGGGACGGGAGCCGC
ACCAGGCAGGCCCTGGTCATCTACTACAGCTTCAACATTGTCTGCTTGGGACTCACCACC
TTGGTCAGCCTGAGCGGCTCCATCATCTACCGTTTTGACCGCCGGGCCATGGACCACCAT
AAGAACCCCACGCGCACTCTGGACGTGGCCCTGCTGATGGGTGCCGCCCTGGGTCAGTAC
GCCATCTCTTACTACTCCATCGTGGCTGTGGTGGCGGGCACACCCCAGGACCTGCTGGCA
GGGCTCAACCTCACCCATGCACTGCTCATGATCGCCCAGCACACCTTCCAGAACATGTTT
ATCATCGAGAGCCTTCACCGAGGACCGCCCGGGGCTGAGCCTCACAGTACCCACCCCAAG
GAGCCCTGCCAAGACCTCACCTTCACCAACCTGGATGCCCTCCACACGTTGTCCGCCTGC
CCACCCAACCCCGGGCTGGTTAGCCCCAGCCCTTCAGACCAGCGGGAAGCAGTGGCCATC
GTCTCAACCCCCAGAAGCCAGTGGAGACGCCAGTGCCTAAAAGACATTTCTCTGTTTCTC
CTACTCTGCAATGTCATTCTGTGGATCATGCCTGCCTTCGGGGCCCGCCCTCATTTCAGC
AACACAGTGGAGGTGGATTTCTACGGCTACTCCCTCTGGGCGGTCATCGTCAACATCTGC
CTCCCTTTCGGCATCTTCTACCGCATGCACGCTGTGTCCAGCCTGCTGGAGGTCTACGTG
CTGTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0371 Download

>KIEE0371  562 aa
MSEELAQGPKESPPAPRAGPREVWKKGGRLLSVLLAVNVLLLACTLISGGAFNKVAVYDT
DVFALLTAMMLLATLWILFYLLRTVRCPCAVPYRDAHAGPIWLRGGLVLFGICTLIMDVF
KTGYYSSFFECQSAIKILHPLIQAVFVIIQTYFLWVSAKDCVHVHLDLTWCGLMFTLTTN
LAIWMAAVVDESVHQSHSYSSSHSNASHARLISDQHADNPVGGDSCLCSTAVCQIFQQGY
FYLYPFNIEYSLFASTMLYVMWKNVGRFLASTPGHSHTPTPVSLFRETFFAGPVLGLLLF
VVGLAVFIIYEVQVSGDGSRTRQALVIYYSFNIVCLGLTTLVSLSGSIIYRFDRRAMDHH
KNPTRTLDVALLMGAALGQYAISYYSIVAVVAGTPQDLLAGLNLTHALLMIAQHTFQNMF
IIESLHRGPPGAEPHSTHPKEPCQDLTFTNLDALHTLSACPPNPGLVSPSPSDQREAVAI
VSTPRSQWRRQCLKDISLFLLLCNVILWIMPAFGARPHFSNTVEVDFYGYSLWAVIVNIC
LPFGIFYRMHAVSSLLEVYVLS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0371 Download

>pFN21AE0371  6391 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCGAGGAGCTGGCCCAGGGCCCCAAGGAGAG
CCCCCCGGCGCCGCGTGCGGGCCCCAGGGAGGTGTGGAAGAAGGGTGGCCGCCTGCTGTC
GGTGCTGCTGGCGGTGAACGTGCTGCTCCTCGCCTGCACGCTCATCAGCGGCGGAGCCTT
CAACAAGGTGGCCGTGTACGACACCGACGTGTTCGCGCTGCTCACTGCGATGATGCTGCT
GGCAACGCTCTGGATCCTCTTCTACCTCCTCCGAACCGTGCGCTGCCCCTGCGCGGTACC
CTACCGGGACGCGCACGCTGGCCCCATCTGGCTCCGAGGTGGGCTGGTGCTGTTTGGAAT
CTGCACCCTCATCATGGATGTCTTCAAGACCGGCTACTACTCCAGTTTCTTTGAGTGCCA
GTCAGCCATCAAGATCCTGCACCCCCTCATCCAGGCTGTGTTTGTCATCATCCAGACCTA
CTTTCTCTGGGTCTCTGCTAAAGACTGCGTTCACGTCCACCTGGATCTGACCTGGTGTGG
TCTCATGTTCACACTCACCACCAACCTGGCCATCTGGATGGCGGCCGTGGTGGATGAATC
TGTGCACCAATCCCACTCCTACAGCAGTTCTCACAGCAACGCCAGCCACGCCCGTCTCAT
CTCTGACCAGCATGCAGACAACCCGGTCGGAGGAGACTCCTGCCTCTGCAGCACGGCCGT
CTGCCAGATCTTCCAGCAGGGGTACTTCTACCTATATCCCTTCAACATCGAGTACAGCCT
CTTCGCCTCCACCATGCTGTATGTCATGTGGAAGAATGTGGGTAGATTCCTGGCCTCCAC
CCCTGGCCACAGCCACACCCCAACCCCTGTCAGCCTCTTCCGGGAGACCTTTTTTGCTGG
CCCGGTTCTGGGCCTGCTGCTCTTCGTGGTGGGGCTGGCTGTCTTCATCATCTACGAGGT
TCAAGTGAGCGGGGACGGGAGCCGCACCAGGCAGGCCCTGGTCATCTACTACAGCTTCAA
CATTGTCTGCTTGGGACTCACCACCTTGGTCAGCCTGAGCGGCTCCATCATCTACCGTTT
TGACCGCCGGGCCATGGACCACCATAAGAACCCCACGCGCACTCTGGACGTGGCCCTGCT
GATGGGTGCCGCCCTGGGTCAGTACGCCATCTCTTACTACTCCATCGTGGCTGTGGTGGC
GGGCACACCCCAGGACCTGCTGGCAGGGCTCAACCTCACCCATGCACTGCTCATGATCGC
CCAGCACACCTTCCAGAACATGTTTATCATCGAGAGCCTTCACCGAGGACCGCCCGGGGC
TGAGCCTCACAGTACCCACCCCAAGGAGCCCTGCCAAGACCTCACCTTCACCAACCTGGA
TGCCCTCCACACGTTGTCCGCCTGCCCACCCAACCCCGGGCTGGTTAGCCCCAGCCCTTC
AGACCAGCGGGAAGCAGTGGCCATCGTCTCAACCCCCAGAAGCCAGTGGAGACGCCAGTG
CCTAAAAGACATTTCTCTGTTTCTCCTACTCTGCAATGTCATTCTGTGGATCATGCCTGC
CTTCGGGGCCCGCCCTCATTTCAGCAACACAGTGGAGGTGGATTTCTACGGCTACTCCCT
CTGGGCGGTCATCGTCAACATCTGCCTCCCTTTCGGCATCTTCTACCGCATGCACGCTGT
GTCCAGCCTGCTGGAGGTCTACGTGCTGTCCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT