TRMT2A ORF clone pFN21AE0342

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Halotag ORF Clone pFN21AE0342

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Clone info.

Species

human

Clone Name

pFN21AE0342 Sequence (with vector) Restriction Map

Product ID

FHC05472

Flexi Clone PID

FXC05472

ORF Sequence

DNA sequence (1875 bp)
Protein sequence (625 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527162]

Gene

KIEE0342

Symbol

TRMT2A

Alias : HTF9C

Description

tRNA methyltransferase 2 homolog A (S. cerevisiae), transcript variant 1

Original Clone ID

oc01042

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1875 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 625 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13774.1 0 100.0 TRMT2A CCDS58793.1 9.1e-199 100.0 TRMT2A CCDS82693.1 8.1e-164 97.2 TRMT2A CCDS14477.1 2.1e-52 37.3 TRMT2B CCDS55464.1 8e-46 42.9 TRMT2B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR010280 401 588 PF05958 (Uracil-5)-methyltransferase ProfileScan IPR000504 73 146 PS50102 RNA recognition motif domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_892029 625 611151 100.0 100.0 NP_073564 625 611151 100.0 100.0 NP_001244923 562 611151 100.0 87.8 NP_001317968 643 611151 97.2 100.0 XP_011528441 643 611151 97.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0342 Download

>KIEE0342  1875 bp
ATGAGTGAGAACCTCGACAACGAGGGCCCGAAGCCCATGGAGAGCTGTGGCCAGGAGAGC
AGCAGTGCCCTGAGCTGCCCTACCGTCTCGGTGCCCCCTGCAGCCCCGGCAGCCCTGGAG
GAGGTGGAGAAAGAGGGCGCTGGGGCGGCTACAGGGCCGGGGCCTCAGCCCGGGCTCTAC
AGCTACATCAGGGATGACTTGTTTACCTCTGAGATCTTTAAACTGGAGCTGCAGAACGTG
CCTCGCCACGCCAGCTTCAGCGACGTCCGGCGCTTCCTGGGCCGCTTTGGTCTGCAGCCC
CACAAAACCAAACTCTTTGGGCAACCACCCTGCGCCTTTGTGACATTCCGCAGCGCTGCA
GAGAGGGACAAGGCCCTGCGCGTTTTGCATGGTGCCCTCTGGAAAGGCCGCCCACTCAGT
GTGCGCCTGGCCCGGCCCAAGGCCGACCCCATGGCCAGGAGGAGGCGACAGGAGGGTGAG
AGTGAGCCACCAGTAACACGAGTGGCCGACGTGGTGACCCCTCTATGGACAGTGCCCTAT
GCTGAGCAGCTTGAGCGGAAGCAGCTGGAGTGCGAGCAGGTGCTGCAGAAACTTGCCAAG
GAAATCGGGAGCACCAACCGTGCCTTGCTGCCCTGGCTGCTCGAGCAGAGGCACAAGCAC
AACAAGGCCTGCTGCCCGCTGGAGGGGGTCAGGCCATCACCCCAGCAGACTGAGTATCGT
AATAAGTGTGAGTTTCTGGTTGGCGTCGGGGTGGATGGGGAGGATAACACCGTGGGCTGT
CGGCTCGGCAAGTACAAGGGCGGGACGTGTGCTGTGGCAGCCCCGTTTGACACCGTGCAC
ATCCCCGAAGCCACCAAGCAGGTGGTGAAGGCCTTCCAGGAGTTCATCCGGTCCACTCCA
TACTCGGCATACGACCCAGAGACGTACACAGGCCACTGGAAGCAGCTGACTGTGCGCACC
AGCCGCCGCCACCAGGCCATGGCCATTGCCTACTTCCACCCCCAGAAGCTGAGCCCTGAG
GAGCTGGCAGAGCTGAAGACCTCCCTAGCGCAGCACTTCACAGCAGGGCCAGGCAGGGCC
AGTGGAGTGACCTGCCTCTACTTCGTGGAGGAGGGACAGCGAAAGACTCCTAGCCAGGAG
GGCCTGCCCCTGGAGCATGTGGCTGGGGACCGGTGCATCCACGAGGACCTGCTAGGGCTG
ACCTTCCGGATCTCTCCACACGCCTTCTTCCAGGTGAACACACCCGCAGCCGAGGTGCTC
TACACAGTCATCCAGGACTGGGCCCAATTGGATGCGGGGAGCATGGTGCTGGACGTGTGC
TGTGGCACCGGCACCATTGGCCTGGCCCTGGCCCGGAAGGTAAAGAGGGTCATTGGGGTC
GAGCTATGCCCAGAGGCTGTGGAGGACGCCCGGGTGAACGCCCAGGACAATGAGTTGAGT
AATGTGGAGTTCCACTGCGGGAGGGCCGAGGACCTGGTGCCCACCCTGGTGAGCAGACTG
GCCTCCCAGCACCTCGTGGCCATCCTGGACCCACCCCGTGCTGGCTTGCATTCCAAGGTG
ATCCTGGCCATCCGGAGAGCTAAGAACCTCAGGCGGCTGCTGTACGTCTCATGCAACCCC
CGGGCAGCCATGGGCAACTTTGTGGACCTCTGCAGAGCCCCATCTAACCGGGTGAAGGGC
ATTCCCTTCCGGCCGGTCAAGGCTGTGGCAGTGGACCTGTTCCCGCAGACCCCGCACTGT
GAGATGCTCATCCTGTTTGAGAGGGTGGAGCACCCCAATGGCACAGGGGTCTTGGGGCCC
CACAGCCCTCCAGCTCAACCCACACCAGGACCCCCAGATAACACCCTACAAGAAACTGGG
ACCTTCCCCTCATCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0342 Download

>KIEE0342  625 aa
MSENLDNEGPKPMESCGQESSSALSCPTVSVPPAAPAALEEVEKEGAGAATGPGPQPGLY
SYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRSAA
ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQEGESEPPVTRVADVVTPLWTVPY
AEQLERKQLECEQVLQKLAKEIGSTNRALLPWLLEQRHKHNKACCPLEGVRPSPQQTEYR
NKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP
YSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRA
SGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVL
YTVIQDWAQLDAGSMVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELS
NVEFHCGRAEDLVPTLVSRLASQHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNP
RAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERVEHPNGTGVLGP
HSPPAQPTPGPPDNTLQETGTFPSS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0342 Download

>pFN21AE0342  6580 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGAGAACCTCGACAACGAGGGCCCGAAGCC
CATGGAGAGCTGTGGCCAGGAGAGCAGCAGTGCCCTGAGCTGCCCTACCGTCTCGGTGCC
CCCTGCAGCCCCGGCAGCCCTGGAGGAGGTGGAGAAAGAGGGCGCTGGGGCGGCTACAGG
GCCGGGGCCTCAGCCCGGGCTCTACAGCTACATCAGGGATGACTTGTTTACCTCTGAGAT
CTTTAAACTGGAGCTGCAGAACGTGCCTCGCCACGCCAGCTTCAGCGACGTCCGGCGCTT
CCTGGGCCGCTTTGGTCTGCAGCCCCACAAAACCAAACTCTTTGGGCAACCACCCTGCGC
CTTTGTGACATTCCGCAGCGCTGCAGAGAGGGACAAGGCCCTGCGCGTTTTGCATGGTGC
CCTCTGGAAAGGCCGCCCACTCAGTGTGCGCCTGGCCCGGCCCAAGGCCGACCCCATGGC
CAGGAGGAGGCGACAGGAGGGTGAGAGTGAGCCACCAGTAACACGAGTGGCCGACGTGGT
GACCCCTCTATGGACAGTGCCCTATGCTGAGCAGCTTGAGCGGAAGCAGCTGGAGTGCGA
GCAGGTGCTGCAGAAACTTGCCAAGGAAATCGGGAGCACCAACCGTGCCTTGCTGCCCTG
GCTGCTCGAGCAGAGGCACAAGCACAACAAGGCCTGCTGCCCGCTGGAGGGGGTCAGGCC
ATCACCCCAGCAGACTGAGTATCGTAATAAGTGTGAGTTTCTGGTTGGCGTCGGGGTGGA
TGGGGAGGATAACACCGTGGGCTGTCGGCTCGGCAAGTACAAGGGCGGGACGTGTGCTGT
GGCAGCCCCGTTTGACACCGTGCACATCCCCGAAGCCACCAAGCAGGTGGTGAAGGCCTT
CCAGGAGTTCATCCGGTCCACTCCATACTCGGCATACGACCCAGAGACGTACACAGGCCA
CTGGAAGCAGCTGACTGTGCGCACCAGCCGCCGCCACCAGGCCATGGCCATTGCCTACTT
CCACCCCCAGAAGCTGAGCCCTGAGGAGCTGGCAGAGCTGAAGACCTCCCTAGCGCAGCA
CTTCACAGCAGGGCCAGGCAGGGCCAGTGGAGTGACCTGCCTCTACTTCGTGGAGGAGGG
ACAGCGAAAGACTCCTAGCCAGGAGGGCCTGCCCCTGGAGCATGTGGCTGGGGACCGGTG
CATCCACGAGGACCTGCTAGGGCTGACCTTCCGGATCTCTCCACACGCCTTCTTCCAGGT
GAACACACCCGCAGCCGAGGTGCTCTACACAGTCATCCAGGACTGGGCCCAATTGGATGC
GGGGAGCATGGTGCTGGACGTGTGCTGTGGCACCGGCACCATTGGCCTGGCCCTGGCCCG
GAAGGTAAAGAGGGTCATTGGGGTCGAGCTATGCCCAGAGGCTGTGGAGGACGCCCGGGT
GAACGCCCAGGACAATGAGTTGAGTAATGTGGAGTTCCACTGCGGGAGGGCCGAGGACCT
GGTGCCCACCCTGGTGAGCAGACTGGCCTCCCAGCACCTCGTGGCCATCCTGGACCCACC
CCGTGCTGGCTTGCATTCCAAGGTGATCCTGGCCATCCGGAGAGCTAAGAACCTCAGGCG
GCTGCTGTACGTCTCATGCAACCCCCGGGCAGCCATGGGCAACTTTGTGGACCTCTGCAG
AGCCCCATCTAACCGGGTGAAGGGCATTCCCTTCCGGCCGGTCAAGGCTGTGGCAGTGGA
CCTGTTCCCGCAGACCCCGCACTGTGAGATGCTCATCCTGTTTGAGAGGGTGGAGCACCC
CAATGGCACAGGGGTCTTGGGGCCCCACAGCCCTCCAGCTCAACCCACACCAGGACCCCC
AGATAACACCCTACAAGAAACTGGGACCTTCCCCTCATCCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT