MIA2 ORF clone pFN21AE0331

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0331

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Clone info.

Species

human

Clone Name

pFN21AE0331 Sequence (with vector) Restriction Map

Product ID

FHC10955

Flexi Clone PID

FXC10955

ORF Sequence

DNA sequence (1962 bp)
Protein sequence (654 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527370]

Gene

KIEE0331

Symbol

MIA2

Alias : CTAGE5, MEA6, MGEA, MGEA11, MGEA6, TALI

Description

melanoma inhibitory activity 2

Original Clone ID

oc01031

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1962 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 654 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9672.1 0 99.5 MIA2 CCDS41470.1 7.1e-15 25.8 MIA3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011511 44 98 PF07653 Variant SH3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0331 Download

>KIEE0331  1962 bp
ATGGCGAAATTTGGCGTTCACAGAATCCTTCTTCTGGCTATTTCTCTGACAAAGTGTCTG
GAGAGTACAAAACTGCTGGCAGACCTTAAAAAATGTGGTGACTTGGAATGTGAAGCTTTA
ATAAACAGAGTCTCAGCCATGAGAGATTATAGAGGACCTGACTGCCGATACCTGAACTTC
ACTAAGGGAGAAGAGATATCTGTTTATGTTAAACTTGCAGGAGAAAGGGAAGATTTGTGG
GCAGGAAGTAAAGGAAAGGAGTTTGGATATTTTCCCAGAGATGCAGTCCAGATTGAAGAG
GTGTTCATATCTGAGGAAATTCAGATGTCAACGAAAGAATCTGACTTTCTTTGTCTTCTT
GGAGTAAGTTACACATTTGACAATGAAGATAGTGAATTAAACGGTGACTATGGTGAAAAT
ATATATCCTTATGAAGAAGATAAAGATGAAAAATCTAGTATATATGAAAGTGATTTTCAG
ATAGAACCTGGATTTTATGCAACTTATGAAAGTACTTTGTTTGAAGACCAAGTTCCAGCA
TTAGAGGCTCCTGAAGATATCGGAAGTACCAGTGAATCAAAAGACTGGGAAGAAGTAGTT
GTTGAAAGTATGGAACAGGATCGTATTCCAGAAGTGCATGTCCCACCATCTTCAGCTGTG
TCTGGAGTCAAAGAATGGTTTGGATTGGGAGGAGAACAAGCTGAAGAGAAGGCTTTTGAA
TCAGTTATTGAACCTGTACAAGAAAGCTCATTTCGGAGTAGAAAAATAGCAGTGGAAGAT
GAGAATGACCTAGAGGAATTAAATAATGGTGAGCCTCAAACAGAACATCAGCAAGAATCT
GAATCAGAAATTGATTCAGTGCCAAGGACACAGTCTGAACTAGCATCTGAGTCAGAGCAC
ATTCCCAAACCTCAATCCACTGGTTGGTTTGGTGGAGGATTTACAAGTTATTCAGGTTTT
GGAGATGAGGATACAGGGCTTGAATTAATAGCTGAAGAAAGCAATCCACCACTACAAGAT
TTTCCCAATTCCATATCATCTGATAAAGAAGCCACAGTTCCATGTACAGAAATATTAACA
GAAAAAAAAGACACAATCACTAATGATAGCTTGAGTCTCAAGCCAAGTTGGTTTGATTTT
GGTTTTGCTATACTAGGCTTTGCATATGCCAAGGAAGATAAAATTATGTTAGATGACAGG
AAAAATGAAGAAGATGGTGGGGCAGATGAACATGAACATCCTCTAACAAGTGAATTAGAC
CCTGAAAAAGAACAAGAAATAGAAACGATAAAAATTATAGAAACAGAAGATCAAATAGAC
AAGAAACCAGTCTCAGAAAAAACAGACGAATCTGATACTATACCATATTTGAAAAAGTTC
TTGTATAATTTTGACAACCCTTGGAACTTCCAGAACATTCCAAAGGAAACAGAATTGCCA
TTTCCCAAACAGATACTGGATCAAAATAATGTAATTGAAAATGAAGAAACTGGAGAATTT
TCCATTGATAATTATCCCACAGATAATACAAAAGTTATGATATTCAAAAGTTCATACAGT
CTGTCAGATATGGTCTCTAACATAGAGTTACCTACGAGAATTCACGAAGAAGTATATTTT
GAACCCTCATCTTCTAAAGATAGTGATGAAAATTCGAAACCATCAGTAGACACCGAAGGG
CCTGCTCTGGTGGAGATAGACAGATCTGTGGAAAATACCCTGCTAAATAGTCAGATGGTT
TCAACTGATAACTCTTTGTCTTCTCAAAATTATATTTCTCAGAAAGAAGATGCTTCTGAG
TTTCAGATTCTGAAATACTTATTCCAAATTGATGTTTATGATTTCATGAATTCTGCATTT
TCACCAATTGTAATTCTTACAGAAAGGGTAAGTTTGCCTTTTAAACCTTTTGCAATAATT
TTACCTATTTTGCTAAATATAAGAGTGGCTGCAAAATATGTT

ORF Protein

Cloned ORF protein sequence for pFN21AE0331 Download

>KIEE0331  654 aa
MAKFGVHRILLLAISLTKCLESTKLLADLKKCGDLECEALINRVSAMRDYRGPDCRYLNF
TKGEEISVYVKLAGEREDLWAGSKGKEFGYFPRDAVQIEEVFISEEIQMSTKESDFLCLL
GVSYTFDNEDSELNGDYGENIYPYEEDKDEKSSIYESDFQIEPGFYATYESTLFEDQVPA
LEAPEDIGSTSESKDWEEVVVESMEQDRIPEVHVPPSSAVSGVKEWFGLGGEQAEEKAFE
SVIEPVQESSFRSRKIAVEDENDLEELNNGEPQTEHQQESESEIDSVPRTQSELASESEH
IPKPQSTGWFGGGFTSYSGFGDEDTGLELIAEESNPPLQDFPNSISSDKEATVPCTEILT
EKKDTITNDSLSLKPSWFDFGFAILGFAYAKEDKIMLDDRKNEEDGGADEHEHPLTSELD
PEKEQEIETIKIIETEDQIDKKPVSEKTDESDTIPYLKKFLYNFDNPWNFQNIPKETELP
FPKQILDQNNVIENEETGEFSIDNYPTDNTKVMIFKSSYSLSDMVSNIELPTRIHEEVYF
EPSSSKDSDENSKPSVDTEGPALVEIDRSVENTLLNSQMVSTDNSLSSQNYISQKEDASE
FQILKYLFQIDVYDFMNSAFSPIVILTERVSLPFKPFAIILPILLNIRVAAKYV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0331 Download

>pFN21AE0331  6667 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGAAATTTGGCGTTCACAGAATCCTTCTTCT
GGCTATTTCTCTGACAAAGTGTCTGGAGAGTACAAAACTGCTGGCAGACCTTAAAAAATG
TGGTGACTTGGAATGTGAAGCTTTAATAAACAGAGTCTCAGCCATGAGAGATTATAGAGG
ACCTGACTGCCGATACCTGAACTTCACTAAGGGAGAAGAGATATCTGTTTATGTTAAACT
TGCAGGAGAAAGGGAAGATTTGTGGGCAGGAAGTAAAGGAAAGGAGTTTGGATATTTTCC
CAGAGATGCAGTCCAGATTGAAGAGGTGTTCATATCTGAGGAAATTCAGATGTCAACGAA
AGAATCTGACTTTCTTTGTCTTCTTGGAGTAAGTTACACATTTGACAATGAAGATAGTGA
ATTAAACGGTGACTATGGTGAAAATATATATCCTTATGAAGAAGATAAAGATGAAAAATC
TAGTATATATGAAAGTGATTTTCAGATAGAACCTGGATTTTATGCAACTTATGAAAGTAC
TTTGTTTGAAGACCAAGTTCCAGCATTAGAGGCTCCTGAAGATATCGGAAGTACCAGTGA
ATCAAAAGACTGGGAAGAAGTAGTTGTTGAAAGTATGGAACAGGATCGTATTCCAGAAGT
GCATGTCCCACCATCTTCAGCTGTGTCTGGAGTCAAAGAATGGTTTGGATTGGGAGGAGA
ACAAGCTGAAGAGAAGGCTTTTGAATCAGTTATTGAACCTGTACAAGAAAGCTCATTTCG
GAGTAGAAAAATAGCAGTGGAAGATGAGAATGACCTAGAGGAATTAAATAATGGTGAGCC
TCAAACAGAACATCAGCAAGAATCTGAATCAGAAATTGATTCAGTGCCAAGGACACAGTC
TGAACTAGCATCTGAGTCAGAGCACATTCCCAAACCTCAATCCACTGGTTGGTTTGGTGG
AGGATTTACAAGTTATTCAGGTTTTGGAGATGAGGATACAGGGCTTGAATTAATAGCTGA
AGAAAGCAATCCACCACTACAAGATTTTCCCAATTCCATATCATCTGATAAAGAAGCCAC
AGTTCCATGTACAGAAATATTAACAGAAAAAAAAGACACAATCACTAATGATAGCTTGAG
TCTCAAGCCAAGTTGGTTTGATTTTGGTTTTGCTATACTAGGCTTTGCATATGCCAAGGA
AGATAAAATTATGTTAGATGACAGGAAAAATGAAGAAGATGGTGGGGCAGATGAACATGA
ACATCCTCTAACAAGTGAATTAGACCCTGAAAAAGAACAAGAAATAGAAACGATAAAAAT
TATAGAAACAGAAGATCAAATAGACAAGAAACCAGTCTCAGAAAAAACAGACGAATCTGA
TACTATACCATATTTGAAAAAGTTCTTGTATAATTTTGACAACCCTTGGAACTTCCAGAA
CATTCCAAAGGAAACAGAATTGCCATTTCCCAAACAGATACTGGATCAAAATAATGTAAT
TGAAAATGAAGAAACTGGAGAATTTTCCATTGATAATTATCCCACAGATAATACAAAAGT
TATGATATTCAAAAGTTCATACAGTCTGTCAGATATGGTCTCTAACATAGAGTTACCTAC
GAGAATTCACGAAGAAGTATATTTTGAACCCTCATCTTCTAAAGATAGTGATGAAAATTC
GAAACCATCAGTAGACACCGAAGGGCCTGCTCTGGTGGAGATAGACAGATCTGTGGAAAA
TACCCTGCTAAATAGTCAGATGGTTTCAACTGATAACTCTTTGTCTTCTCAAAATTATAT
TTCTCAGAAAGAAGATGCTTCTGAGTTTCAGATTCTGAAATACTTATTCCAAATTGATGT
TTATGATTTCATGAATTCTGCATTTTCACCAATTGTAATTCTTACAGAAAGGGTAAGTTT
GCCTTTTAAACCTTTTGCAATAATTTTACCTATTTTGCTAAATATAAGAGTGGCTGCAAA
ATATGTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT