LIPK ORF clone pFN21AE0256

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0256

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Clone info.

Species

human

Clone Name

pFN21AE0256 Sequence (with vector) Restriction Map

Product ID

FHC10902

Flexi Clone PID

FXC10902

ORF Sequence

DNA sequence (1197 bp)
Protein sequence (399 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528790]

Gene

KIEE0256

Symbol

LIPK

Alias : LIPL2, bA186O14.2

Description

lipase, family member K

Original Clone ID

oc00956

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1197 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 399 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44455.1 3.8e-186 100.0 LIPK CCDS7389.1 5e-120 63.0 LIPF CCDS55718.1 5.1e-120 63.0 LIPF CCDS7401.1 1.9e-104 57.2 LIPA CCDS31240.1 2.7e-102 59.3 LIPJ The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006693 35 96 PF04083 AB-hydrolase-associated lipase region IPR000073 111 383 PF00561 Alpha/beta hydrolase fold-1
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MWQLLAAACWMLLLGSMY 18 PRIMARY 18 2 83 QHGLIASASNWICNLPNNSLAFL 105 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011538379 399 613922 100.0 100.0 NP_001073987 399 613922 100.0 100.0 XP_011538377 408 613922 100.0 100.0 XP_011538375 419 613922 100.0 100.0 XP_016872037 358 613922 92.4 81.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0256 Download

>KIEE0256  1197 bp
ATGTGGCAGCTTTTAGCAGCAGCATGCTGGATGCTTCTTCTTGGATCTATGTATGGTTAT
GACAAGAAAGGAAACAATGCAAACCCTGAAGCTAATATGAATATTAGCCAGATTATTTCT
TACTGGGGTTATCCTTATGAAGAGTATGATGTTACAACAAAAGATGGTTATATCCTTGGA
ATTTATAGGATTCCACATGGAAGAGGATGCCCAGGGAGGACAGCTCCAAAGCCTGCTGTG
TATTTGCAGCATGGCTTAATTGCATCTGCCAGTAACTGGATTTGCAACCTGCCCAACAAC
AGTTTGGCTTTCCTTCTGGCAGATAGTGGTTATGACGTGTGGTTGGGGAACAGCCGAGGA
AACACTTGGTCCAGAAAACACCTTAAATTGTCACCGAAATCACCGGAATACTGGGCCTTC
AGTTTGGATGAGATGGCTAAATATGACCTTCCAGCCACAATCAATTTTATCATAGAGAAA
ACTGGACAGAAGCGACTCTACTACGTGGGCCACTCACAAGGCACCACCATAGCTTTTATA
GCATTTTCTACAAACCCAGAACTGGCTAAAAAGATTAAGATATTTTTTGCACTGGCTCCA
GTTGTCACAGTTAAATACACCCAAAGTCCTATGAAAAAACTAACAACCCTTTCCAGGCGA
GTAGTTAAGGTGTTGTTTGGTGACAAAATGTTCCACCCTCATACATTGTTTGACCAATTC
ATTGCCACCAAAGTGTGCAATCGAAAGCTATTCCGTCGTATTTGCAGCAACTTCCTATTT
ACTCTGAGTGGATTTGATCCGCAAAACTTAAATATGAGTCGCTTGGATGTTTATTTGTCA
CACAATCCTGCGGGAACATCTGTTCAGAATATGCTGCACTGGGCTCAGGCTGTTAATTCT
GGTCAGCTCCAAGCTTTTGATTGGGGAAACTCTGATCAGAACATGATGCACTTCCATCAG
CTTACACCTCCTTTATACAACATTACTAAGATGGAAGTTCCAACAGCAATATGGAATGGT
GGACAGGACATTGTGGCTGATCCCAAGGATGTTGAAAATTTACTTCCTCAAATTGCTAAC
CTTATTTATTACAAGCTGATTCCACACTACAATCATGTGGATTTTTACCTTGGAGAGGAT
GCACCTCAGGAAATTTACCAAGACCTAATTATATTGATGGAAGAATATTTACAAAAT

ORF Protein

Cloned ORF protein sequence for pFN21AE0256 Download

>KIEE0256  399 aa
MWQLLAAACWMLLLGSMYGYDKKGNNANPEANMNISQIISYWGYPYEEYDVTTKDGYILG
IYRIPHGRGCPGRTAPKPAVYLQHGLIASASNWICNLPNNSLAFLLADSGYDVWLGNSRG
NTWSRKHLKLSPKSPEYWAFSLDEMAKYDLPATINFIIEKTGQKRLYYVGHSQGTTIAFI
AFSTNPELAKKIKIFFALAPVVTVKYTQSPMKKLTTLSRRVVKVLFGDKMFHPHTLFDQF
IATKVCNRKLFRRICSNFLFTLSGFDPQNLNMSRLDVYLSHNPAGTSVQNMLHWAQAVNS
GQLQAFDWGNSDQNMMHFHQLTPPLYNITKMEVPTAIWNGGQDIVADPKDVENLLPQIAN
LIYYKLIPHYNHVDFYLGEDAPQEIYQDLIILMEEYLQN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0256 Download

>pFN21AE0256  5902 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGGCAGCTTTTAGCAGCAGCATGCTGGATGCT
TCTTCTTGGATCTATGTATGGTTATGACAAGAAAGGAAACAATGCAAACCCTGAAGCTAA
TATGAATATTAGCCAGATTATTTCTTACTGGGGTTATCCTTATGAAGAGTATGATGTTAC
AACAAAAGATGGTTATATCCTTGGAATTTATAGGATTCCACATGGAAGAGGATGCCCAGG
GAGGACAGCTCCAAAGCCTGCTGTGTATTTGCAGCATGGCTTAATTGCATCTGCCAGTAA
CTGGATTTGCAACCTGCCCAACAACAGTTTGGCTTTCCTTCTGGCAGATAGTGGTTATGA
CGTGTGGTTGGGGAACAGCCGAGGAAACACTTGGTCCAGAAAACACCTTAAATTGTCACC
GAAATCACCGGAATACTGGGCCTTCAGTTTGGATGAGATGGCTAAATATGACCTTCCAGC
CACAATCAATTTTATCATAGAGAAAACTGGACAGAAGCGACTCTACTACGTGGGCCACTC
ACAAGGCACCACCATAGCTTTTATAGCATTTTCTACAAACCCAGAACTGGCTAAAAAGAT
TAAGATATTTTTTGCACTGGCTCCAGTTGTCACAGTTAAATACACCCAAAGTCCTATGAA
AAAACTAACAACCCTTTCCAGGCGAGTAGTTAAGGTGTTGTTTGGTGACAAAATGTTCCA
CCCTCATACATTGTTTGACCAATTCATTGCCACCAAAGTGTGCAATCGAAAGCTATTCCG
TCGTATTTGCAGCAACTTCCTATTTACTCTGAGTGGATTTGATCCGCAAAACTTAAATAT
GAGTCGCTTGGATGTTTATTTGTCACACAATCCTGCGGGAACATCTGTTCAGAATATGCT
GCACTGGGCTCAGGCTGTTAATTCTGGTCAGCTCCAAGCTTTTGATTGGGGAAACTCTGA
TCAGAACATGATGCACTTCCATCAGCTTACACCTCCTTTATACAACATTACTAAGATGGA
AGTTCCAACAGCAATATGGAATGGTGGACAGGACATTGTGGCTGATCCCAAGGATGTTGA
AAATTTACTTCCTCAAATTGCTAACCTTATTTATTACAAGCTGATTCCACACTACAATCA
TGTGGATTTTTACCTTGGAGAGGATGCACCTCAGGAAATTTACCAAGACCTAATTATATT
GATGGAAGAATATTTACAAAATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT